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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for PI 3 Kinase p110 delta Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
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bioRxiv - Microbiology 2022Quote: ... The full Delta-Omicron recombinant spike was cloned into pcDNA6 (Invitrogen). Point mutations were introduced by overlap extension PCR ...
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bioRxiv - Cell Biology 2022Quote: ... Vinculin Rabbit mAb (# 700062; Invitrogen), p-H3 Rabbit mAb (S10 ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant GST-tagged kinase active human mTOR (Cat. PV4753, ThermoFisher) was used instead of the immunoprecitated mTORC1 ...
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bioRxiv - Microbiology 2022Quote: ... rabbit mAb (#4394); IKKβ (D30C6) rabbit mAb (#8943); phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) rabbit mAb (#2078; blocked with SuperBlock (Thermo Scientific)) ...
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bioRxiv - Microbiology 2020Quote: Recombinant human mAbs were expressed in Expi293 HEK cells (Life Technologies), which were maintained in suspension at 37°C and 8% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Recombinant mAbs were then produced in EXPi293F cells (Life Technologies, USA) by transfecting pairs of the IgG1 heavy and light chain expression plasmids ...
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bioRxiv - Immunology 2023Quote: Recombinant mAbs were transiently produced in FreeStyle 293F cells (ThermoFisher Scientific) following the protocol detailed by Vink et al (Vink ...
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bioRxiv - Microbiology 2021Quote: ... and anti-PGK (yeast 3-phosphoglycerate kinase, Invitrogen) antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Systems Biology 2021Quote: ... secondary mAB anti-goat HRP (rabbit, Invitrogen, 611620), secondary mAB anti-mouse (sheep ...
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bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
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bioRxiv - Microbiology 2022Quote: ... NF-kB2 p100/p52 (D7A9K) rabbit mAb (#37359); phospho-NF-κB2 p100 (Ser866/870) rabbit mAb (#4810; blocked with SuperBlock (Thermo Scientific)) ...
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bioRxiv - Immunology 2023Quote: ... delta-surface plates (Nunc) were pre-coated with 12 μg/ml polyclonal anti-hamster IgG (MP Biomedicals ...
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bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with recombinant murine IL-3 (Gibco, PMC0035) at 1 μg/mL (BaF3) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10ng/ml recombinant human IL-3 (Gibco). Cultured cells were infected with lentivirus encoding GFP/Scrambled control shRNA overnight and fresh media was added the following day ...
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bioRxiv - Cancer Biology 2024Quote: ... beads were incubated in kinase reaction buffer supplemented with 100 ng recombinant CAMK2D (Invitrogen, cat. PV3373), 100 µM recombinant calmodulin (Enzo ...
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bioRxiv - Cell Biology 2022Quote: ... Guide RNA primer sets A (KS40 5’-ACCGACCACAGAAACTGCGACTAG -3’ and KS41 5’-AACCTAGTCGCAGTTTCTGTGGTC-3’) and B (KS42 5’-CCGTCCCACTGGCACCGCTTTATG-3’ and KS43 5’-AAACATAAAGCGGTGCCAGTGGGA-3’) were phosphorylated with T4 polynucleotide kinase (ThermoFisher) at 37°C for 30 min and annealed by cooling down from 85°C to 25°C at 0.1°C/sec ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 5-μm frozen sections of uterine myometrium embedded in OCT compound were obtained using Leica CM1510 S cryostat and immunostained for phosphorylated myosin light chain kinase (1:200 rabbit anti-mouse phosphomyosin light chain kinase, Invitrogen) and imaged with the Zeiss Axioskop 40 microscope ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified cell supernatant containing recombinant mAb was passed over Protein A Agarose resin (Thermo Fisher Scientific). Protein A resin was extensively washed with 25 mM Phosphate pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... PI (Invitrogen) was added immediately before flow cytometric analysis on an Attune NxT Cytometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... PI (Invitrogen), CD19 PE-Cy7 (Biolegend) ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit recombinant ANTI-FLAG M2 antibody (Invitrogen 710662), guinea pig anti-bovine insulin (Linco ...
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bioRxiv - Bioengineering 2021Quote: ... with 3 μL of Syto 9 and Propidium iodide (PI) dyes (ThermoFisher Scientific, USA) 1000-fold diluted in PBS (MilliporeSigma ...
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bioRxiv - Biochemistry 2021Quote: ... After adding 3 μL of 10 U/mL PI-PLC (P6466, Thermo Fisher Scientific) diluted in buffer B ...
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bioRxiv - Microbiology 2021Quote: ... Delta-delta CQ was calculated using the 18S ribosomal RNA primer/probe set (4331182; Thermo Fisher). Virus RNA (vRNA ...
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bioRxiv - Developmental Biology 2024Quote: ... Gene expression was calculated and normalized to GAPDH level using delta-delta Ct method (Applied Biosystems). Full primer sequences were listed on Table S4 ...
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bioRxiv - Immunology 2023Quote: ... Delta S was synthesised by Invitrogen GeneArt (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Nunclon delta surface plates (Thermo Scientific) were coated with 100 µl of 5 µg/ml of M1 ...
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bioRxiv - Microbiology 2021Quote: ... were incubated with the MprF- or lysozyme-specific humanized IgG mAB and/or a GFP-specific rabbit IgG mAB (Invitrogen) for 30 min shaking at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng/mL recombinant mouse interleukin-3 (Gibco PMC0031).
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bioRxiv - Cell Biology 2021Quote: ... ISG15 recombinant rabbit monoclonal antibody (1□00) (Invitrogen, 703132), and collagen I (1□100 ...
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bioRxiv - Immunology 2020Quote: ... PI (Life Technologies) at 16.6μg/ml in staining buffer was added directly to samples in RNase A (final density of 106 cells in 650μl) ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was collected 6 days later and recombinant mAb was purified using protein A agarose (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Bioengineering 2023Quote: ... and Col1A1 (E8F4L) XP Rabbit mAb cell signaling (Catalog 72026, ThermoFisher Scientific) with Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... 12 Well Nunclon Delta Surface (Thermo Scientific), 96 Well Tissue Culture Plate (VWR 734-2327) ...
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bioRxiv - Neuroscience 2021Quote: ... Nunc Delta surface treated plates (Thermo Scientific) were coated as previously described (24) ...
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bioRxiv - Immunology 2021Quote: ... and Delta RBD with PE-streptavidin (Invitrogen). PBMCs were thawed at 37 °C and immediately washed with DMEM supplemented with 2% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and/or propidium Iodide (PI) 10 μg/mL (PI, P3566, Invitrogen) were then added ...
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bioRxiv - Genetics 2020Quote: ... 3-Phosphoglycerate kinase (Pgk1) protein was detected with anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) and HSP90 protein was detected with a mouse anti-HSP90 monoclonal antibody (1:2000 ...
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bioRxiv - Genetics 2020Quote: ... 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Genetics 2022Quote: ZsGreen and flag-tagged ADAR1-p110 mRNA were made using the Invitrogen MEGAscript T7 Transcription Kit (Fisher Scientific, Cat #: AM1334) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... extracellular signal-regulated kinase kinase (MEK, Ambion: Cat. no. s11167), Akt1 (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor 647-conjugated anti-β-tubulin (9F3) rabbit mAb (Thermo Fisher Scientific) to visualize cilia ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... AHR (1 μg/ml, Rabbit mAb JM34-10, Thermo Fisher Scientific MA5-32576), CYCLIN B1 (1:10,000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Propidium Iodide (PI, Invitrogen) was added to sample before sorting to gate viable cells.
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bioRxiv - Cancer Biology 2021Quote: ... PI (Thermo Fisher Scientific) was added to the cell suspension ...