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Citations for Thermo Fisher :
1 - 50 of 7420 citations for PCSK9 Human V474I G670E HEK293 His HA Avi since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Avi/His-tagged proteins were purified from transiently-transfected Expi293F cells (Gibco) by nickel affinity chromatography and SEC (Barnes et al. ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-mouse PCSK9 (Invitrogen), mouse anti-mouse vinculin (Santa Cruz) ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney (HEK293, Thermo Fisher Scientific) cells ...
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bioRxiv - Bioengineering 2021Quote: ... rat monoclonal anti-Pcsk9 (MA524144, Invitrogen), antibodies ...
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bioRxiv - Microbiology 2020Quote: ... and the Human embryonic kidney (HEK293) cells (Gibco) in Freestyle F17 expression medium (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: Human derived HEK293 cells (Invitrogen, Inchinnan, UK, R75007) stably transfected with cAMP GloSensor™ (pGloSensor-22F ...
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bioRxiv - Neuroscience 2023Quote: Grip TiteTM Human Embryonic Kidney (GT HEK293) (Invitrogen) cells were grown as previously described (Chałupnik et al ...
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bioRxiv - Neuroscience 2023Quote: Human Embryonic Kidney Grip TiteTM (HEK293-GT) cells (Invitrogen) was used to create a polyclonal cell line expressing GluA2(Q)i as previously described [49] ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Immunology 2023Quote: RAVV GPΔMuc fused with Strep II and Avi tags was expressed in HEK293S GNTI−/− cells (ATCC, Manassas, VA) using 293fectin transfection reagent and FreeStyle Media (Thermo Fisher Scientific, Waltham, MA). Supernatants were purified using Streptactin XT Resin purification (IBA Lifesciences ...
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bioRxiv - Immunology 2021Quote: Human embryonic kidney cells (HEK293) were maintained in IMDM (Gibco) supplemented with 10% FCS and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: Human Embryonic Kidney (HEK293) T-Rex Flp-In (ThermoFisher Scientific) cells were grown in DMEM supplemented with 10% FBS in an atmosphere of 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human embryonic kidney cell lines (HEK293) containing a Flp-In expression vector (HEK293 Flp-In) obtained from ThermoFisher Scientific were utilized in this study ...
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bioRxiv - Biophysics 2021Quote: ... Human embryonic kidney 293 cells (HEK293) were maintained in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) were grown in DMEM/F12 media (GIBCO) supplemented with HyClone cosmic calf serum (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: Human embryonic kidney (HEK293) cells were cultured in DMEM (Thermofisher Scientific) containing 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... Human Embryonic Kidney cells (HEK293-CD19+) were cultured in DMEM (Gibco), 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Synthetic Biology 2019Quote: ... An anti-6His antibody (6x-His Tag Polyclonal Antibody) and an anti-HA antibody (HA Tag Polyclonal Antibody) were purchased from Invitrogen. Western blot analysis was conducted as described previously 45.
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bioRxiv - Developmental Biology 2022Quote: ... pMT-yki-HA was generated by cloning Yki-RG and HA tag into pMT-V5-His vector (Invitrogen, Cat# V412020). The Y281A ...
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bioRxiv - Genetics 2020Quote: HEK293 and human fibroblast culture medium consisted of DMEM (Gibco; 11995-065) supplemented with 10% fetal bovine serum (Gibco 16000-036) ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney (HEK293; Invitrogen’s Flp-In-293 cell line cat #R75007) cells were grown and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Biochemistry 2022Quote: HEK293 (Human Embryonic Kidney 293-Flp-In T-Rex, Thermo Fisher Scientific) cells were cultured in DMEM (Dulbecco’s modified Eagle‘s medium ...
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bioRxiv - Systems Biology 2023Quote: Different human cell lines (HeLa, HEK293) were cultured in DMEM (Gibco, Invitrogen), supplemented with 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: HEK293 human kidney cells were cultured in D-MEM (Thermo Fisher Scientific), supplemented with 5% HyClone Cosmic Calf serum (Cytiva ...
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bioRxiv - Systems Biology 2023Quote: Different human cell lines (HeLa, HEK293) were cultured in DMEM (Gibco, Invitrogen), supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2020Quote: ... HA-tagged RON was cloned into the expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) by fusion PCR ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were transfected with pcTBK1-His or pcGSK3B-Flag plasmid using Lipofectamine 2000 (Invitrogen, Waltham, MA, US) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: Human embryonic kidney (HEK293) cells were maintained in Dulbecco’s modified eagle medium (Gibco) containing 10% fetal bovine serum (HyClone) ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney cells (HEK293) were cultured in Dulbecco’s modified Eagle’s media (Gibco) containing 10 % v/v fetal bovine serum (heat-inactivated ...
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bioRxiv - Biochemistry 2020Quote: ... Human 28S and 18S rRNAs were extracted from HEK293 cells with TRIzol (Invitrogen). Intact rRNAs were isolated by pipetting from a native agarose gel after running the rRNA into wells in the center of the gel ...
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bioRxiv - Physiology 2022Quote: ... HEK293 cells were transfected with human TRPA1 expressing plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK293, ATCC #CRL-1573) cells were cultured in DMEM (Gibco), supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2021Quote: HEK293/TLR4 and HEK293/null cells (both Invitrogen) were maintained in 4.5 g/L glucose DMEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 human embryonic kidney cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Invitrogen), containing 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney293 (HEK293) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Life Technologies) supplemented with 10% fetal bovine serum (Atlanta biologicals ...
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bioRxiv - Cell Biology 2021Quote: Human HEK293 suspension culture cells (Expi293F) were cultured in Expi293F expression media (Fisher Scientific) and maintained at 37°C in 8% CO2 in 125 mL spinner flasks on an orbital shaker rotating at 125 rpm ...
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bioRxiv - Biochemistry 2022Quote: The human embryonic kidney HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution at 37 °C under 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: Human HEK293 cells were cultured at 37 °C and 5% CO2 in DMEM (ThermoFisher Scientific), supplemented with 10% fetal bovine serum (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: Human embryonic kidney 293 (HEK293) cells were grown in high glucose DMEM (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... The vectors were then transfected into human embryonic kidney cells (HEK293) with Fectin-system (Invitrogen). Protein from culture supernatant was isolated as previously described [46 ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney fibroblasts (HEK293, RRID:CVCL_0045) were grown in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher) supplemented with 10% fetal calf serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... All human MARCH cDNAs were cloned into pCDNA3.1/myc-His A (Invitrogen) and were then subcloned into pBJ5 ...
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bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
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bioRxiv - Molecular Biology 2019Quote: ... Fluorescently labeled wild-type PCSK9 was prepared using the DyLight800 Antibody Labeling Kit (ThermoFisher) as per manufacturer’s instructions followed by gel filtration chromatography on a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 T-Rex (referred to as HEK293, cat.no. R71007, Invitrogen) and NIH3T3 cells were grown in DMEM Glutamax® (Thermo Fisher Scientific ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... HEK293S GnT1-/- or HEK293 cells (ThermoFisher Scientific, Cat. No. R78007) using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells (Invitrogen) were grown in DMEM (1g/L glucose ...
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bioRxiv - Synthetic Biology 2022Quote: HEK293 cells (ThermoFisher; further authenticated by assessing cell morphology and growth rate ...