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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Norovirus GII.4 VLP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Norovirus VLPs were labelled with an Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) dissolved in DMSO at a concentration of 10 µg/mL ...
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bioRxiv - Microbiology 2020Quote: ... VLP-WT and VLP-cvD were expressed using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: VLP and scFc-VLPs (4µM) were applied onto a glow-discharged carbon-supported copper TEM grid (TEM-CF200CU50, Thermo Fisher Scientific) and incubated for 60 s before gently removing the solution ...
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bioRxiv - Immunology 2023Quote: Proteins in VLP containing samples were separated by SDS-PAGE using 4-12% Bis-Tris Nu-PAGE gels (Invitrogen) and electro-transferred to a PVDF membrane using the Trans-Blot Turbo Transfer Pack (BioRad) ...
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bioRxiv - Evolutionary Biology 2019Quote: Proteins extracted from purified VLPs were using Laemmli buffer were stacked in the top of a SDS-PAGE gel (4-12% NuPAGE, Life Technologies), stained with Coomassie blue R-250 and in-gel digested using modified trypsin (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was extracted from the lysed VLPs using TriReagent (Ambion) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... VLPs were resuspended in Life cell imaging solution (Thermo Fisher) and stored at − 80°C ...
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bioRxiv - Microbiology 2020Quote: ... VLPs were incubated with AnnexinV-PE conjugate (AnV-PE) (Invitrogen) in AnV binding buffer for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The purified VLP fraction was treated with DNase I (Ambion) at 37 °C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Pelleted VLPs were resuspended in 1X NuPAGE LDS sample buffer (ThermoFisher). Cell lysates were collected by washing cells twice with PBS followed by lysis in CytoBuster ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted VLPs were resuspended in 1X NuPAGE LDS sample buffer (ThermoFisher). Cell lysates were collected by washing cells twice with PBS followed by lysis in CytoBuster (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were buffer exchanged into DPBS (Thermo Fisher Scientific Cat #: 14190-144) using a 100-kDa Amicon filter (Sigma ...
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bioRxiv - Immunology 2019Quote: Chimeric VLPs or monomeric recombinant proteins were coated onto Maxisorp microtiter plates (Nunc) at 1μg/ml in PBS and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Enriched VLPs were lysed with 10% SDS and 20mg/mL Proteinase K (Ambion) at 56C° for 20 minutes ...
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bioRxiv - Biophysics 2023Quote: 50 µl of AP205 VLP solution (5 µg/ml) was pipetted into an appropriate number of wells of ELISA plates (4 Nunc MaxiSorp flat bottom 96-well plates) and the VLP allowed to adsorb overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... For the overexpression of SERPINB2, pLENTI-GII-CMV-GFP-2A-Puro (abm, #434730610395) was transfected in fibroblasts using Lipofectamine 2000 (Invitrogen, #11668019).
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bioRxiv - Microbiology 2019Quote: ... VLPs were produced using the Bac-to-Bac Baculovirus Expression System (Invitrogen, California, USA) and purified through a cesium chloride gradient as previously described (63) ...
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bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... VLPs were incubated for 2 hours in the DNAse and RNAse cocktails (Invitrogen, Carlsbad, CA) with the addition of 14 µL of 20 mg/ml Proteinase K (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS) (Thermo Fisher Scientific #: 14190-144) using a 100-kDa Amicon filter (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were expressed with the Bac-to-Bac TOPO Expression system (Invitrogen, Catalog nos. A11101, A11100) according to instructions from the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Stained VLPs were image with Talos L120 transmission electron microscope (TEM, Thermo Fisher Scientific, former FEI) with 47000x magnification ...
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bioRxiv - Immunology 2023Quote: ... PCSK9 peptides were conjugated to Qß bacteriophage VLPs using succinimidyl 6-((ß-maleimidopropionamido)hexanoate) (SMPH; Thermo Fisher Scientific), as described previously60 ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: VLPs were produced in adherent HEK-293 [HEK-293](ATCC-CRL-1573) or the suspension Expi293 cells Expi293 (Gibco). HEK-293 cells were maintained in 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Immunology 2024Quote: ... The protein concentration of the purified VLPs was measured using a Pierce™ BCA Protein Assay kit (Thermo Fisher). VLPs were then flash frozen in liquid nitrogen and stored at −80 °C until use ...
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bioRxiv - Bioengineering 2023Quote: Concentrated EV or VLP total protein concentrations were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) These neutralization reagents were mixed with 4X loading dye ...
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bioRxiv - Microbiology 2024Quote: ... HEK293FT (293T cells optimized for VLP production, gift from N. Manel) and OMK cells were cultivated in media DMEM (Thermofisher) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: The ESP collector surface for VLP deposition was modified to integrate two 50 μL collection wells (Thermo Fisher Scientific AB0620). The wells were metallized with gold using a sputter deposition system (CVC SC-4000 RF Magnetron Sputtering System ...
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bioRxiv - Biophysics 2023Quote: ... the solution of stained VLP was buffer exchanged with pure PBS using Zeba™ Spin Desalting Columns (7,000 MWCO, ThermoFisher). We note that since the columns contained sodium azide ...
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bioRxiv - Immunology 2020Quote: ... Reactivity of the CIS43 mAb for the CIS43 VLPs was determined by measuring optical density at 450□nm (OD450) using an AccuSkan platereader (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Total prokaryotic cells and VLP abundance in 100 μL samples were determined using an Attune NxT Acoustic Focusing Flow Cytometer (ThermoFisher Scientific) by their signature in a plot of green fluorescence versus side scatter (78 ...
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bioRxiv - Immunology 2020Quote: SEC-purified HA-VLPs and HA-NPs were prepared on grids for cryo-ET using a Mark IV Vitrobot (ThermoFisher Scientific) operated at 21°C and 100% humidity ...
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bioRxiv - Immunology 2020Quote: ... The peptide was conjugated directly to surface lysines on Qβ bacteriophage VLPs using the bidirectional crosslinker succinimidyl 6-[(beta-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as previously described 47 ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... FP8-GGGC peptides were conjugated to Qβ-VLPs using the bifunctional cross-linker succinimidyl 6-[(β-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as described previously for the conjugation of amyloid-beta (Aβ ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing either dCas9-KRAB or a targeting sgRNA were generated by transfection of HEK293 cells with Lipofectamine 2000 (Thermo Fisher Scientific). Cells were cotransfected with the packaging plasmid pCMV-dR8.91 ...
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bioRxiv - Immunology 2021Quote: ... and was conjugated directly to surface lysines on Qβ bacteriophage VLPs using the bidirectional crosslinker succinimidyl 6-[(beta-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as previously described 10 ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Extracted nucleic acids from VLPs and two extraction blanks were used for cDNA synthesis using the SuperScript™ IV First-Strand Synthesis System (Invitrogen™) with random hexamers provided by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Both viral particles and VLP were labelled with Alexa Fluor®647 protein labelling kit according to manufacturer’s instructions (Molecular Probes, Eugene, OR). Modified-vaccinia Ankara virus (MVA ...
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bioRxiv - Microbiology 2023Quote: We mixed 2 μl of EEEV VLP at a concentration of 1.6 mg ml-1 in DPBS (Thermo Fisher Scientific Cat #: 14190-144) with 2 μl of VLDLRLBD-Fc in TBS supplemented with 2 mM CaCl2 at a concentration of 1.6 mg ml-1 ...
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bioRxiv - Neuroscience 2020Quote: ... Fluo-4 (Fluo 4 AM/Fluo 4 direct, Life Technologies) was used according to the manufacturer’s instructions and a previously published protocol (54) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... with 4% sucrose/4% formaldehyde (ThermoFisher Scientific) in 1X PBS ...
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bioRxiv - Genetics 2023Quote: ... 4 mM 4-acetamido-4’- maleimidylstilbene-2,2’-disulfonate (AMS) (Invitrogen, catalogue no. A485), and then keep in the dark at room temperature for 1.5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 4 μM Mag-Fluo-4 (ThermoFisher Scientific) and 1 μM F-127 (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Biochemistry 2021Quote: ... Succinimidyl 6-(4, 4’-azipentanamido)hexanoate (LC-SDA; Thermo Scientific) crosslinking was performed by adding the reagent to final 1mM concentration ...
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bioRxiv - Neuroscience 2022Quote: ... A glass coverslip (4×4 mm, #1.5 thickness; Thermo Scientific) was positioned over two-thirds of the craniotomy ...
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bioRxiv - Biophysics 2023Quote: ... 7-Diethylamino-3-(4’-Maleimidylphenyl)-4-Methylcoumarin (CPM) (Invitrogen. USA), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC ...