Labshake search
Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Mouse anti Ebola Virus envelope glycoprotein GP Zaire M922 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... plasmid encoding myc- and His-tagged full-length EBOV GP (Zaire ebolavirus isolate Ebola virus/H.sapiens-wt/SLE/2014/Makona-G3838) using Lipofectamine™ 3000 (Thermo Fisher Scientific Cat. Nr. L3000) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ...
-
bioRxiv - Biophysics 2023Quote: ... Ebola GP expression plasmid kindly provided by Jochen Bodem) using Lipofectamine™ 3000 (ThermoFisher) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: The Zaire EBOV GP gene was expressed using a pcDNA vector under control of the CMV promoter (Invitrogen, Carlsbad, CA) (54 ...
-
bioRxiv - Genetics 2022Quote: ... Tissues were incubated with mouse anti-P-glycoprotein C219 antibody at 3.6μg/mL (Glycoprotein Monoclonal Antibody C219, Cat. No MA1-26528, Invitrogen) overnight at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
-
bioRxiv - Cell Biology 2022Quote: ... and 2.16 μg of the vector expressing the VSV-G envelope glycoprotein (pMD2.G) were mixed with OptiMEM (Gibco) to a final volume of 400 μl ...
-
bioRxiv - Cancer Biology 2023Quote: ... along with psPAX2 and pMD2.G packaging and envelope virus using lipofecctamine 3000 (Invitrogen) in serum-free Opti-MEM media (Invitrogen) ...
-
Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... Cleaved GP was detected with the primary mouse RBS-specific anti-GP antibody MR72 followed by secondary anti-mouse antibody-Alexa 555 fluorophore conjugate (Thermo Scientific). To investigate transport of IgGs bound to VSV particles into NPC1+ endosomal compartments ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were immunostained with an anti-EBOV GP mouse antibody (IBT, MD, USA) and Alexa Fluor 488 anti-mouse secondary (Thermo Fisher, MA, USA). Nuclei were counterstained with Hoechst 33342 ...
-
bioRxiv - Neuroscience 2022Quote: ... secondary donkey anti-GP AF 594 (1:500; Invitrogen) antibody was applied to neurons for 1.5 h at 20°C.
-
bioRxiv - Cell Biology 2021Quote: ... and 4 μg of plasmid VSV-G encoding G glycoprotein of vesicular stomatitis virus(VSVG) using Lipofectamine 3000 Reagent (Invitrogen). 12 h later ...
-
bioRxiv - Cancer Biology 2022Quote: Glycoproteins were isolated using a glycoprotein isolation kit (89804; Thermo Fisher Scientific) as per the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Goat anti-GP IgG Alexa Fluor 488 (Molecular Probes A11073), goat anti-guinea pig IgG Alexa Fluor 647 (Molecular Probes A-21450) ...
-
bioRxiv - Microbiology 2020Quote: SADS-CoV spike glycoprotein (virus strain GDS04; GenBank No.: ASK51717.1) gene was synthesized with codons optimized and inserted into pFastBac vector (Life Technologies Inc.). The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068 ...
-
bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A Virus M1 (1:1,000; clone: GA2B; Cat#: MA1-80736; Invitrogen), mouse anti-Influenza A virus M2 (1:1,000 ...
-
bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A virus M2 (1:1,000; clone: 14C2; Cat#: MA1-082; Invitrogen), mouse anti-GAPDH (1:1,000 ...
-
bioRxiv - Biochemistry 2022Quote: ... was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... pEco envelope was used to produce virus to infect murine T cells) using lipofectamine 2000 (Cat. No. 11668019, Invitrogen, Carlsbad, California, USA) on a 100□mm2 poly-D-lysine–coated plate (Corning ...
-
bioRxiv - Immunology 2022Quote: ... glycoproteins were digested with trypsin (ThermoFisher) overnight at 37°C ...
-
bioRxiv - Biochemistry 2020Quote: ... and Pierce Glycoprotein Staining kit (ThermoFisher) were used for protein and glycan staining ...
-
bioRxiv - Biochemistry 2021Quote: ... while the other was stained for glycoproteins using the Pro-Q Emerald 300 glycoprotein staining kit (Invitrogen) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... A glycoprotein ladder (Candy Cane ladder; Invitrogen) served as positive control ...
-
bioRxiv - Microbiology 2024Quote: ... along with a CandyCane glycoprotein ladder (Thermofisher). We additionally loaded the same two B ...
-
bioRxiv - Microbiology 2020Quote: ... rVSV-BOMV GP and THL-cleaved GPs were instead resolved in Bolt 4–12% gradient gels (ThermoFisher), with EBOV GPs for comparison ...
-
bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
-
bioRxiv - Bioengineering 2022Quote: ... P-gp (Thermo Fisher Scientific; p170(F4)) ...
-
bioRxiv - Systems Biology 2020Quote: ... and a second part was stained and analysed for glycoproteins using the Pro-Q 488 Emerald Glycoprotein staining kit (P33375, Thermo/Invitrogen), according to the manufacturers protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... secondary donkey anti-GP AF 594 antibody was applied to coverslips at 1:500 (Invitrogen) for 1.5 h at 20°C.
-
bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
-
bioRxiv - Developmental Biology 2021Quote: ... and GP-647 (Thermo Fisher Scientific #A-21450).
-
bioRxiv - Cell Biology 2020Quote: ... and GP-647 (Thermo Fisher Scientific #A-21450).
-
bioRxiv - Immunology 2021Quote: ... The lentiviral envelope vector pLP/VSVG (Invitrogen) and the packaging vector psPAX2 (Trono lab ...
-
bioRxiv - Molecular Biology 2020Quote: ... and envelope plasmid in OptiMEM (Life Technologies) for 5 to 6 hours (PEI:total pDNA μg ratio 2:1) ...
-
bioRxiv - Developmental Biology 2022Quote: ... or with Pierce™ Glycoprotein Staining Kit (Thermo Scientific) after separation.
-
bioRxiv - Biochemistry 2019Quote: ... pcDNA3.1P-gp was constructed by the insertion of cDNA encoding human P-gp in pcDNA3.1 (Thermo Fisher Scientific, Austin, TX, USA). To engineer P-gp–Venus ...
-
bioRxiv - Microbiology 2021Quote: ... that co-localises with IBDV virus factories (16) and a goat anti-mouse secondary antibody conjugated to Alexa-Fluor 488 (Invitrogen). Wells were scored as positive or negative based on the presence of infected cells ...
-
bioRxiv - Microbiology 2021Quote: ... The attachment of HIV-1 virus produced in the presence of TMEM123 was also separately tested by pre-incubating the virus with mouse monoclonal anti-TMEM123 antibody (297617) (ThermoFisher) (25ug/ml ...
-
bioRxiv - Immunology 2022Quote: ... and Plat-GP cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
-
bioRxiv - Plant Biology 2022Quote: ... pastoris-produced LysM protein solution was tested using a protein polyacrylamide gel followed by CBB and glycoprotein staining with the Pierce Glycoprotein Staining Kit (Thermo Fisher Scientific, California, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2019Quote: Antibodies and antibody Fabs were produced as described previously.57 BG505 N332 SOSIP gp140 envelopes were expressed as previously described with minor modifications.58 Envelope production was performed with Freestyle293 cells (Invitrogen). On the day of transfection ...
-
bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
-
bioRxiv - Immunology 2020Quote: ... Pro-Q™ Emerald 300 glycoprotein gel stain kit (Invitrogen) was used to detect the presence of sugar in Bl-Eng2 before and after deglycosylation ...
-
bioRxiv - Cell Biology 2022Quote: ... pCMV-gp and pCMV-vsvg using Lipofectamine 2000 (#10696153, Fisher Scientific) in Opti-MEM medium (#11520386 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 260 ng pMD.G envelope plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.25 µg envelope plasmid diluted in 100 µl optiMEM (Gibco) was mixed with 20 µl of PolyFect transfection reagent (Qiagen ...
-
bioRxiv - Immunology 2023Quote: ... mouse anti-mouse PCSK9 (Invitrogen), mouse anti-mouse vinculin (Santa Cruz) ...
-
bioRxiv - Biochemistry 2019Quote: ... P-gp constructs were transfected using Lipofectamine LTX Reagent (Thermo Fisher Scientific), and the transfected cells were incubated for an additional 24 h ...