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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Mac 1 SAP mouse human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 0.5 µl of SAP (Affymetrix), 0.1 µl E ...
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bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP, Affymetrix Cat # 78390) and Sanger sequenced.
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bioRxiv - Genomics 2023Quote: ... 1 ul of EXO-SAP-IT Express (Life Technologies-75001.1.ML) was added to the reaction ...
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bioRxiv - Neuroscience 2022Quote: ... Then Shrimp Alkaline Phosphatase (SAP) reaction (total volume 10 µl) was performed with 10× SAP buffer (Affymetrix), 0.5 µl of SAP (Affymetrix) ...
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bioRxiv - Developmental Biology 2022Quote: ... purified by Exo-Sap-IT (Affymetrix, 78201) to remove unincorporated primer ...
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bioRxiv - Microbiology 2019Quote: ... DNA was treated with Exo-SAP-IT (ThermoFisher) and incubated at 37°C for 15 min followed by a 15 min incubation at 80°C to inactivate the enzyme ...
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bioRxiv - Genomics 2020Quote: ... with excess primers removed with EXO-SAP-IT (Affymetrix), and then amplified samples were diluted 1/5 in DNA suspension buffer ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Neuroscience 2019Quote: ... polyclonal rabbit anti-mouse/human ZO-1 (1:200; Life Technologies), monoclonal rat anti-mouse ICAM-1 (1:100 ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR products were purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis (through Genewiz) ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis ...
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bioRxiv - Immunology 2021Quote: ... 1:100 mouse anti-human CXCR5 PE-Cy7 (MU5UBEE, ThermoFisher) was added to each well at the start of stimulation ...
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bioRxiv - Genomics 2019Quote: ... amplicons were cleaned with Exo-SAP (Affymetrix, Santa Clara, CA, USA), quantified with QuantiFluor dsDNA system (Promega ...
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bioRxiv - Immunology 2022Quote: ... mouse-anti-mouse/human/rat monoclonal PP1a (1:1000 dilution, number MA5-17239, ThermoFisher Scientific), rabbit-anti-Y.pestis polyclonal YopM (1:1000 dilution ...
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bioRxiv - Developmental Biology 2020Quote: Primary prenatal human microglia were MACS-purified as described above and labeled with DiI (Thermo Fisher, V22885) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-Human/Mouse Myod1 (5.8A) (1:100, Thermo Fisher Scientific, MA512902), Anti-Mouse Myosin Heavy Chain (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... polyclonal rabbit anti-mouse/human claudin-5 (1:300; Life Technologies), polyclonal rabbit anti-mouse/human ZO-1 (1:200 ...
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bioRxiv - Biochemistry 2021Quote: ... monoclonal mouse anti-human Rpt6 (PSMC5) (Invitrogen, 2SU-1B8, 1:10000), monoclonal mouse anti-human Rpt4 (Enzo ...
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bioRxiv - Biophysics 2022Quote: ... or monoclonal anti-human NPM1 (IgG1, mouse, 1:500) (325200, Invitrogen) in blocking/permeabilization buffer for 120 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... anti-Human/Mouse MyoD (5.8A) (1:200, Thermo Fisher Scientific, MA512902) and anti-myogenin (F5D ...
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bioRxiv - Neuroscience 2024Quote: ... FITC anti-human/mouse CD45R (1:500, #11-0452-82, Invitrogen), PE anti-mouse CD3ε (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320, CellBlox monocyte/MAC blocking buffer, Invitrogen #B001T07F01). Cells were stained in an antibody cocktail containing the appropriately titrated antibodies described in Supplementary Table S3 for 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... MAC-BNIP3 and MAC-NIX were cloned into pcDNA5/FRT/TO (Thermo Fisher). Constructs were co-transfected with pOG44 into HeLa-T-rex Flp-in cells to generate inducible cell lines using Flippase (Flp ...
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bioRxiv - Microbiology 2019Quote: ... 2 µl of EXO-SAP (100 U shrimp alkaline phosphatase (Thermo Scientific), 100 U Exonuclease I (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons were cleaned using Exo-SAP-IT (Affymetrix, Santa Clara, CA, USA), and Sanger-sequenced at the Core Genomic Facility at the University of Sheffield ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products were treated with Exo-Sap II (Thermo Fisher Scientific), and sequence termination reactions were performed from both directions of strands using BigDye Terminator v3.2 (Applied Biosystem) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed two times with 200 ul cold MACS buffer and resuspended in 200 ul cold MACS buffer with 1:3000 propidium iodide (Thermo Fisher Scientific). Immunofluorescence staining was analyzed using a Cytoflex (Beckman Coulter) ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed two times with 200 ul cold MACS buffer and resuspended in 200 ul cold MACS buffer with 1:3000 propidium iodide (Thermo Fisher Scientific). Immunofluorescence staining was analyzed using a Cytoflex (Beckman Coulter) ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Plant Biology 2021Quote: ... in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen; Carlsbad, CA, USA) for 1 hr at 37°C ...
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bioRxiv - Genomics 2021Quote: ... samples were then filtered using a 30 mM MACS strainer (MACS strainer, Fisher Scientific), centrifuged at 500xg for 5 minutes at 4°C using a swinging bucket rotor (Sorvall Legend RT ...
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bioRxiv - Neuroscience 2020Quote: ... monoclonal mouse anti-human α-synuclein (1:1000, 4B12, Thermo Fisher Scientific); the goat anti-ChAT (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-human α-synuclein 1:1000 (Thermo Scientific; Rockford, IL, USA); goat anti-luciferase 1:5000 (Abcam ...
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bioRxiv - Pathology 2021Quote: ... and mouse monoclonal anti-human VCAM1 (dilution 1:25, clone 1.4C3, Invitrogen) for 1h at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... monoclonal anti-human lamin B2 (IgG2a, mouse, 1:100) (MA5-17274, Invitrogen), polyclonal anti-human MPO (IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in MACS buffer containing 1 μM SYTOX Blue (Invitrogen) and processed by fluorescence-activated cell sorting (FACS ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-human CD68 (Invitrogen, 10987212), mouse anti-human CLEC7A (R&D Systems ...
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bioRxiv - Genomics 2023Quote: The double-stranded linkers were converted into single-stranded to successively allow the second strand synthesis by Shrimp Alkaline Phosphatase (1 U/μl SAP, catalog num. 78390, Affymetrix) and Uracil-Specific Excision Reagent (1 U/μl USER ...
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bioRxiv - Immunology 2021Quote: Human CD4 T cells were MACS-sorted from PBMC and subsequently pre-activated with α-CD3/CD28 Dynabeads (Thermofisher) at a bead-to-cell ratio of 1:1 for 48 hours ...
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bioRxiv - Bioengineering 2021Quote: ... anti-CD81 (1:300, mouse monoclonal anti-human CD81 antibody, Invitrogen, MA5-13548); anti-CD9 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with human anti-mouse ZO-1 (AlexaFluor-488 conjugate; ThermoFisher)(5 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-human α-synuclein 1:1000 (Thermo Scientific; Rockford, IL, USA) or goat anti-luciferase 1:250 (Novus Biological ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were probed with mouse anti-human TAU (HT7; 1:50,000; Invitrogen MN1000), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... Embryos were stained with mouse-anti-human-ZO-1 1:500 (Thermo Fisher Scientific, 33-9100) or guinea pig-anti-zebrafish-VE-cadherin 1:500 (Paatero ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were treated with Exo-Sap (ExoSAP-IT™, Thermo Fisher, USA) to remove unutilized primers and dNTPs ...
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bioRxiv - Microbiology 2021Quote: Purified substrates and cleavage product mixtures were dephosphorylated by incubating with SAP (ThermoFisher, USA) at 37°C for 1 h according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2021Quote: ... we purified the amplified DNA using USB Exo-SAP-IT (Affymetrix, Santa Clara, CA) and bidirectionally sequenced the amplicons using an ABI 3130 DNA Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... the resulting DNA from the PCR reaction was purified via EXO-SAP cleanup (Affymetrix). Individual EXO-SAP reactions consisted of 1.55ul dH2O ...
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bioRxiv - Systems Biology 2023Quote: ... 1 vein graft slide containing 2-4 cross sections was double stained with the general macrophage marker Mac-3 (rat anti-mouse, Fisher Scientific, B550292, 1:600) and either iNOS (rabbit anti-mouse ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-human aSyn (Life Technologies, #180215) at 1:200 dilution ...