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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for MOA SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 0.5 µl of SAP (Affymetrix), 0.1 µl E ...
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bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP, Affymetrix Cat # 78390) and Sanger sequenced.
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bioRxiv - Neuroscience 2022Quote: ... Then Shrimp Alkaline Phosphatase (SAP) reaction (total volume 10 µl) was performed with 10× SAP buffer (Affymetrix), 0.5 µl of SAP (Affymetrix) ...
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bioRxiv - Developmental Biology 2022Quote: ... purified by Exo-Sap-IT (Affymetrix, 78201) to remove unincorporated primer ...
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bioRxiv - Microbiology 2019Quote: ... DNA was treated with Exo-SAP-IT (ThermoFisher) and incubated at 37°C for 15 min followed by a 15 min incubation at 80°C to inactivate the enzyme ...
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bioRxiv - Plant Biology 2021Quote: Xylem sap protein concentrations were determined with Pierce® Coomassie Plus kit (Thermo Fisher Scientific), which is based on the Bradford method (Bradford ...
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bioRxiv - Microbiology 2023Quote: ... MOA cells were first removed from plates by washing with PBS then trypsinization with 0.05% trypsin (Gibco). Trypsin was quenched with cell culture media ...
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bioRxiv - Genomics 2020Quote: ... with excess primers removed with EXO-SAP-IT (Affymetrix), and then amplified samples were diluted 1/5 in DNA suspension buffer ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR products were purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis (through Genewiz) ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis ...
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bioRxiv - Genomics 2019Quote: ... amplicons were cleaned with Exo-SAP (Affymetrix, Santa Clara, CA, USA), quantified with QuantiFluor dsDNA system (Promega ...
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bioRxiv - Genomics 2023Quote: ... 1 ul of EXO-SAP-IT Express (Life Technologies-75001.1.ML) was added to the reaction ...
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bioRxiv - Microbiology 2019Quote: ... 2 µl of EXO-SAP (100 U shrimp alkaline phosphatase (Thermo Scientific), 100 U Exonuclease I (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons were cleaned using Exo-SAP-IT (Affymetrix, Santa Clara, CA, USA), and Sanger-sequenced at the Core Genomic Facility at the University of Sheffield ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products were treated with Exo-Sap II (Thermo Fisher Scientific), and sequence termination reactions were performed from both directions of strands using BigDye Terminator v3.2 (Applied Biosystem) ...
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bioRxiv - Genetics 2021Quote: ... The PCR products were purified with 3 μl Exo/SAP and then cycle-sequenced with the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to manufacturer instructions with BP15851 (M13F ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were treated with Exo-Sap (ExoSAP-IT™, Thermo Fisher, USA) to remove unutilized primers and dNTPs ...
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bioRxiv - Microbiology 2021Quote: Purified substrates and cleavage product mixtures were dephosphorylated by incubating with SAP (ThermoFisher, USA) at 37°C for 1 h according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2021Quote: ... we purified the amplified DNA using USB Exo-SAP-IT (Affymetrix, Santa Clara, CA) and bidirectionally sequenced the amplicons using an ABI 3130 DNA Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... the resulting DNA from the PCR reaction was purified via EXO-SAP cleanup (Affymetrix). Individual EXO-SAP reactions consisted of 1.55ul dH2O ...
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bioRxiv - Microbiology 2021Quote: ... followed by purification using the Exo-SAP-IT PCR product clean-up reagent (Thermofisher, Australia) according to manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... after enzymatic clean up with Exo-SAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) applying a 10x dilution of the Exo-SAP-IT but otherwise following the manufactureŕs instructions ...
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bioRxiv - Plant Biology 2021Quote: ... in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen; Carlsbad, CA, USA) for 1 hr at 37°C ...
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bioRxiv - Genomics 2023Quote: The double-stranded linkers were converted into single-stranded to successively allow the second strand synthesis by Shrimp Alkaline Phosphatase (1 U/μl SAP, catalog num. 78390, Affymetrix) and Uracil-Specific Excision Reagent (1 U/μl USER ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting cDNA underwent another final second round IVT step at 37 °C overnight and followed EXO-SAP treatment (Affymetrix 78200) for 15 minutes at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... the aRNA was treated with 6 μL EXO-SAP (ExoSAP-IT™ PCR Product Cleanup Reagent, Thermo Fisher Scientific, Cat. # 78200.200.UL) at 37°C for 15 min followed by fragmentation with 5.5 μL fragmentation buffer (200 mM Tris-acetate (pH 8.1) ...
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bioRxiv - Plant Biology 2021Quote: 5 μg of RNA from 5-day-old seedlings was first treated with 1 unit of Shrimp Alkaline Phosphatase (SAP; USB Products, Affymetrix, Inc.; Cleveland, OH, USA) in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... One millilitre of fresh sap or control sample was freeze-dried using a SpeedVac concentrator unit (model Savant SPD121P, Thermo Scientific, Waltham, MA, USA) at 40 □ ...
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bioRxiv - Bioengineering 2023Quote: DNA assay kit QuantiT dsDNA HS kit (Invitrogen) was used to quantify the DNA content from cell lysate according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2023Quote: ... following kit instructions (BCA Protein Assay kit, ThermoFisher #23225) and 20µg loaded into each well of a NuPAGE 4-12% Bis-Tris Gel (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... mitochondria isolation kit and IL-6 kit were from Invitrogen and were purchased from ALT Ukraine Ltd (representative of Thermo Fisher Scientific in Ukraine).
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bioRxiv - Molecular Biology 2023Quote: ... kit and a TURBO DNA-free Kit (Thermo Fisher Scientific), the RNA was then further purified using RNeasy kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Kit (ThermoFisher Scientific) and subsequently 10 clones per cell line were sequenced (LightRun GATC Carlo Erba) ...
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bioRxiv - Genetics 2020Quote: ... coli kit (Invitrogen). The transfection was performed according to the manufacturer’s guidelines and vector assembly was tested by Sanger sequencing.
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bioRxiv - Systems Biology 2022Quote: A kit (Ambion Magmax™-96 total RNA isolation kit ...
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bioRxiv - Physiology 2023Quote: Transcription Kit (Invitrogen). The qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Life Technologies) ...
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bioRxiv - Biophysics 2023Quote: Kit(Ambion AM1340M). They were injected at the one-cell stage together with the microspheres:
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bioRxiv - Molecular Biology 2021Quote: ... PCR cloning kits from Thermo Scientific (TOPO TA Cloning Kit 450030) and New England Biolabs ...
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bioRxiv - Synthetic Biology 2020Quote: ... GeneJET gel extraction kit and plasmid miniprep kit (Thermo Fisher Scientific) were used for DNA extraction ...
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bioRxiv - Genetics 2021Quote: A commercially available kit (Click-iT EdU Cell Proliferation Kit, ThermoFisher) was used to label proliferating cells with thymidine analog 5-ethynyl-2’- deoxyuridine (EdU) ...
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bioRxiv - Microbiology 2021Quote: ... The commercially available kit (ThermoFisher, CyQUANT™ LDH Cytotoxicity Assay Kit) was used according to manufacturer protocol ...
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bioRxiv - Pathology 2023Quote: ... A magnetic bead sorting kit (CELLection Pan Mouse IgG Kit, Invitrogen) was used according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Guide RNAs were expressed and purified using commercial kits (MEGAscript T7 Transcription Kit, Invitrogen and mirVana miRNA isolation Kit, Invitrogen); stored in ddH2O at 0.6-1 mg/ml at −80°C and thawed 1 h prior to the experiment ...
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bioRxiv - Molecular Biology 2020Quote: ... supernatants were diluted 4-fold and assayed with the Amyloid beta 40 Human ELISA Kit and either the Amyloid beta 42 Human ELISA Kit or the Amyloid beta 42 Human ELISA Kit Ultrasensitive (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the GeneJET Gel Extraction Kit (ThermoFisherScientific, Vilnius, Lithuania) and quantified using a fluorometric RiboGreen kit (Qubit Assay Kit, Invitrogen, Life Technologies Corporation ...
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bioRxiv - Plant Biology 2020Quote: ... Blots were developed using an alkaline phosphatase kit (BCIP/NBT kit; Invitrogen).
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bioRxiv - Immunology 2020Quote: ... The following ELISA kits were used: IL-1β Mouse ELISA kit (ThermoFisher), IL-18 Mouse ELISA kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... stained using BacLight viability Kit (L7007, BacLight Bacterial Viability Kit, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... MEGAshortscript T7 kit and mirVana miRNA isolation kit were purchased from Invitrogen Australia ...
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bioRxiv - Microbiology 2021Quote: ... PureLink Genomic DNA kits or MagMAX Cell-Free DNA kits (ThermoFisher Scientific) according to the manufacturer’s instructions ...