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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for MCP 1 CCL2 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The levels of CCL2 (MCP-1) were quantified with mouse CCL2 ELISA kit (Thermo Fisher Scientific, Cat. Nr. BMS6005) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... with the Mouse Monocyte Chemoattractant Protein-1/CCL2 (MCP-1) Uncoated ELISA Kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The CCL2 levels in tumor lysate was measured by MCP-1 Mouse Uncoated ELISA Kit (Thermo Scientific, #88-7391-22) with comparable sized tumors (∼3 mm diameter) ...
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bioRxiv - Cell Biology 2020Quote: ... TNF and MCP-1 respectively (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CCL5 and CCL2 (Human cytokine magnetic 25-plex panel; Life technologies).
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bioRxiv - Immunology 2020Quote: ... MCP-1 and CXCL1 (Invitrogen, Carlsbad, CA). Electrochemiluminescence ELISAs (Meso Scale Diagnostics ...
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bioRxiv - Bioengineering 2021Quote: ... CCL2 was quantified using a rat CCL2 ELISA (Cat. # KRC1012 Life Technologies, Carlsbad, CA, USA) according to manufacturer’s instructions and buffer modifications described in Yarmola et al (29) ...
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bioRxiv - Bioengineering 2021Quote: ... and CCL2 was quantified using a rat CCL2 ELISA kit (Cat. # KRC1012 Life Technologies, Carlsbad, CA), according to the manufacturer’s instructions and modifications described in (35) ...
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bioRxiv - Cell Biology 2020Quote: Cell lines used in this study were THP-1 (human monocytic leukemia) cells, HeLa (Henrietta Lacks, human cervical carcinoma cells, CCL2) and HEK293T Flp-In T-REx 293 (Thermo Fisher Scientific). THP-1 cells were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Plant Biology 2022Quote: Total RNA extraction and synthesis of complementary DNA (cDNA) from MCPs (approximately 400 MCPs) were performed using a SuperScript IV CellsDirect cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... IL-18 and TNF-α levels were determined by ELISA kit (ThermoFisher, for MCP-1) and (Affymetrix eBioscience ...
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bioRxiv - Pathology 2021Quote: ... inducible nitric oxide synthase (NOS2) and monocyte chemoattractant protein-1 (CCL2) were purchased from Applied Biosystems. Gene expression levels were determined with the comparative Ct quantitation method using Hypoxanthine-guanine phosphoribosyltransferase (Hprt1 ...
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bioRxiv - Plant Biology 2021Quote: Plasmids directing the expression of 3xFLAG tagged CCL2 or CCL2-Y92A under the control of the CaMV 35S promoter were constructed using the Gateway Cloning Technology (Thermo Fisher Scientific, San Jose, USA). The open reading frames of CCL2 and CCL2-Y92A were PCR-amplified from respective E ...
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bioRxiv - Pathology 2019Quote: ... The MCP-3 (Cat#: BMS6006INST) ELISA kit were from Invitrogen. The GCP-2 (Cat# ...
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bioRxiv - Immunology 2019Quote: ... and stained with MCP (FITC, eBiosciences) and HSP (APC, Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: Cytokine quantification was completed using mouse CCL2 (Invitrogen# 88-7391-22) and mouse CCL7 (Invitrogen# BMS6006INST ...
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bioRxiv - Immunology 2021Quote: ... The bottom chamber of the plate contained PBS with 20 ng/well monocyte chemoattractant protein-1 (MCP-1, Thermofisher Scientific, USA). Macrophages (5×104 cells in PBS ...
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bioRxiv - Immunology 2021Quote: ... and CCL2 were measured using Mouse Uncoated ELISA Kit (Invitrogen, Vienna, Austria) and mouse CXCL1/KC DuoSet ELISA (R&D systems ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells (CCL2 from ATCC) were cultured in DMEM medium (Gibco BRL) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... Primers not listed (CD36, CD206, and MCP-1) were purchased prepackaged from Life Technologies (Grand Island, NY, USA) or Amersham ...
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bioRxiv - Molecular Biology 2022Quote: ... TGF-β1 and monocyte chemotactic protein-1 (MCP-1) was measured using the ELISA kit following the manufacturer’s protocol (eBioscience Affymetrix, Santa Clara,CA, United States). The levels of Muc5ac and Muc5b in BALF were also measured according to Lu et al ...
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bioRxiv - Physiology 2023Quote: ... Each cDNA sample was amplified with messenger RNA (mRNA) specific TaqMan Gene Expression Assays (IL-6, Mm00446190_m1; MCP-1, Mm00441242_m1; GAPDH, Mm99999915_g1; Thermo Fisher Scientific, Waltham, MA) on a CFX-96 real-time polymerase chain reaction (PCR ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with 10 µg pPB-LR5-CAG-MCP-mNeonGreen-IRES-Neo and 1 µg pCMV-hyPBase vectors using Lipofectamine 2000 (Invitrogen 11668019). After incubation for 2 days ...
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bioRxiv - Neuroscience 2023Quote: ... cat#740344) and MCP-1(B9, cat#740345) multiplex beads were sonicated for two minutes in a sonicator bath (Thermo Fisher, USA). These multiplex beads were then appropriately diluted in assay buffer and added to a V-bottomed plate ...
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bioRxiv - Cell Biology 2020Quote: ATCC HeLa CCL2 (ATCC) were cultured at 37°C and 5% CO2 in DMEM (Gibco 11965092) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% human IgG (Invitrogen) in PBS] followed by incubation with primary antibodies ...
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bioRxiv - Genetics 2019Quote: ... human COT-1 DNA (Invitrogen) and biotin-labeled mouse COT-1 or minor satellite DNA (a gift from Dr ...
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bioRxiv - Genetics 2021Quote: ... human COT-1 DNA (ThermoFisher) to detect HSA21 and biotin-labeled I-EGFP-I-loxP-3’HPRT (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Human Cot-1 DNA (Invitrogen), 3M NaAc and 100% cold EtOH ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells (ATCC CCL2) were cultured in EMEM (ATCC 30-2003) containing 10 % fetal bovine serum (Invitrogen 16140071) and 1 % Penicillin/Streptomycin (Invitrogen 15140122) ...
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bioRxiv - Molecular Biology 2022Quote: MCP-tagged SARS-CoV-2 expression plasmids for the MS2 tethering assay were generated by Gateway Assembly (ThermoFisher). SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Pathology 2023Quote: ... CCL2 levels were measured in conditioned media by ELISA (Invitrogen, cat nos. 88-7391-88 and 88-7399-88).
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bioRxiv - Neuroscience 2020Quote: ... goat anti-human (ThermoFisher, 1:1000). Hoechst staining (ThermoFisher ...
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bioRxiv - Genomics 2022Quote: ... or human cot-1 (15279011, ThermoFisher), and 0.6 μl of 100 μM TIME-Seq hybridization blocking primers (IDT) ...
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bioRxiv - Microbiology 2022Quote: ... 1:2,000 goat anti-human (Invitrogen), 1:1,000 goat anti-mouse (Invitrogen ...
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bioRxiv - Synthetic Biology 2019Quote: ... The total protein concentration of MCP samples was measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 1.25 μg of each pcDNA5/FRT/TO/i/FLAG/MCP-based plasmid and 1.25 μg of pOG44 plasmid encoding recombinase were transfected using Lipofectamine LTX (Invitrogen). Cell lines with stably integrated constructs were selected with 150 μg/ml hygromycin (Wako) ...
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bioRxiv - Immunology 2022Quote: ... CCL2 was affinity purified from the in vitro reactions using Phytip streptavidin coated tips (Phynexus) bound to biotinylated anti-CCL2 antibody (Invitrogen) using the Phytip protocol ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor 546, #6006661-01, probes were made against the sequence between mouse Ccl2 gene map positions 2-785, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... primers are listed in Supplemental Table 3), Luminex using a custom procarta Luminex 6-plax-cytokines (IL1β, IL6, IL8, IL18, CCL2, and TNFa) detection kit (ThermoFisher) (Supplemental Fig ...
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bioRxiv - Cell Biology 2024Quote: HeLa cells (ATCC, CCL2) were grown at 37°C at 5% CO2 in Dulbecco’s modified eagle medium (Gibco 10566-016) supplemented with 10% FBS (Gibco A31604-01) ...
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bioRxiv - Plant Biology 2021Quote: ... driving eGFP expression derived from pUBN-Dest26 was assembled with MCP contained in pDONR2018 using the Gateway cloning technique (Invitrogen) creating plasmid pUb::eGFP/MCP-NLS ...
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bioRxiv - Molecular Biology 2023Quote: HeLa cells were seeded in 12-well plates at 80% confluency and transfected with 250 ng GFP-THAP1-MS2 and 250 ng of the MCP-mCherry fusion constructs indicated in the figure (Lipofectamine 3000, Invitrogen). When indicated ...
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bioRxiv - Cell Biology 2022Quote: ... 1% human keratinocyte growth supplement (HKGS, Gibco) and 1% pen-strep ...
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bioRxiv - Immunology 2022Quote: ... Sheep anti-human FH (1:200×, ThermoFisher) and then goat anti-sheep HRP (1:2000× ...
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bioRxiv - Cell Biology 2020Quote: ... Human Cot-1 (DNA) (Thermo Fisher Scientific) (10 μg per 2 μg of nick translated BAC vector ...
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bioRxiv - Neuroscience 2023Quote: ... anti-human IgG (Invitrogen, A21445, 1:400).
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bioRxiv - Cell Biology 2023Quote: ... or human (1:2000; Thermo Fisher Scientific). DAPI was used to stain DNA (500 ng/mL ...
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bioRxiv - Microbiology 2023Quote: ... 1:2500 goat anti-human Ab (Invitrogen), 1:5000 goat anti-rabbit Ab (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... 1:1000 anti-human-AF594 (Molecular Probes) and 1:2000 L/D eFluor506 (Thermo Fischer Scientific ...