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Citations for Thermo Fisher :
1 - 50 of 1840 citations for Legionella pneumophila antigen native extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... pneumophila (Invitrogen, PA17227), 1:2,000 rat α-LAMP1 (Developmental Studies Hybridoma Bank ...
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bioRxiv - Immunology 2022Quote: ... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
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bioRxiv - Cell Biology 2020Quote: ... Legionella polyclonal antibody (Invitrogen, Cat: PA1-7227) was used at 1:2,000 and incubated for 20 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... pneumophila primary antibody (Invitrogen; PA17227) and 1:500 Alexa488-conjugated goat α-rabbit secondary antibody (ThermoFisher) ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila antibody (Invitrogen, PA17227; 1:1000) at RT ...
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bioRxiv - Microbiology 2022Quote: ... pneumophila antibody [1:1,000 (Invitrogen; PA17227)] in DMEM 10%HIFBS supplemented with 1 mM IPTG for 20 min at room temperature (RT ...
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bioRxiv - Cell Biology 2021Quote: ... native protein extracts were mixed with 4x sample buffer (Thermo Scientific, Cat#BN2003) and water (final sample buffer 1x ...
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bioRxiv - Molecular Biology 2022Quote: ... The indicated amount of total protein extract was mixed with native sample buffer from Invitrogen. Following the electrophoresis (150 V ...
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bioRxiv - Genetics 2019Quote: ... Twenty micrograms of whole-cell extracts were loaded on 3-12% gradient native-PAGE gels (Invitrogen) and subsequently subjected to a 16-h electrophoresis at 45 V using a running buffer consisting of 50 mM BisTris/Tricine ...
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bioRxiv - Systems Biology 2022Quote: ... 0.8 mg of native proteome extract (estimated by Pierce™ BCA Protein Assay Kit, Thermo Fisher Scientific, MA, USA) was injected for each SEC run at 4 °C with a flow rate of 500 μl/min ...
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bioRxiv - Immunology 2020Quote: ... perfringens native ECP or native CWP were coated on ELISA plates (Nunc MaxisorpTM ...
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bioRxiv - Immunology 2022Quote: ... Blue-Native PAGE was carried out using th Native-PAGE system (ThermoFisher) with 10-20μg of total lysate ...
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bioRxiv - Immunology 2024Quote: ... Whole-cell extracts (1e6 cells) were solubilized with 2 mg of digitonin in native PAGE sample buffer (Thermo Fisher Scientific, BN2008) for 30 min on ice ...
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bioRxiv - Biochemistry 2021Quote: ... with samples for blue native-PAGE solubilised in Native PAGE sample buffer (ThermoFisher) containing 1% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μL of native marker (Native Marker unstained protein standard, LC0725, Life Technologies) diluted 400 x in the GF buffer was used for mass/contrast calibration that was monitored during 60 s using the AcquireMP software (Refeyn Ltd ...
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bioRxiv - Cell Biology 2022Quote: ... bacteria were diluted in complete media and mixed with a Legionella polyclonal antibody (Invitrogen, Cat #PA1-7227) at 1:1,000 dilution ...
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bioRxiv - Immunology 2022Quote: ... whole cell extracts were prepared by solubilization of 106 cells with 2 mg digitonin and 1X Native PAGE sample buffer (Thermo Fisher, BN2008) on ice for 30 min ...
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bioRxiv - Microbiology 2021Quote: Samples for native PAGE were diluted in 4X Native PAGE Sample Buffer (ThermoFisher, BN2003) and loaded into wells of 4-16% Native PAGE Bis-Tris acrylamide gels (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... The native loading dye was Novex Tris-Glycine Native Sample Buffer (Thermo Fisher Scientific). SMA-PAGE gels were run at 150 V for 90-120 mins at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Native Mark Protein standard (Invitrogen) was used as the molecular weight marker ...
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bioRxiv - Cell Biology 2022Quote: ... Native mark protein standard (Invitrogen) was used as the molecular mass marker ...
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bioRxiv - Cell Biology 2019Quote: ... Blue Native Page was performed using a Novex Native PAGE Bis-Tris Gel System (Invitrogen) following manufacturer protocols ...
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bioRxiv - Cell Biology 2022Quote: Blue Native Page was performed using a Novex Native PAGE Bis-Tris Gel System (Invitrogen) following manufacturer protocols ...
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bioRxiv - Microbiology 2022Quote: ... 6X RNA native loading buffer (Ambion) was added to stop the reaction and resolved on a 6% native PAGE at 4⁰C in 0.5% TBE at a constant current of 40 mA for 6 h ...
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bioRxiv - Immunology 2020Quote: ... perfringens native ECP or native CWP were coated on ELISA plates (Nunc MaxisorpTM, ThermoFisher Scientific, Waltham, MA) at 10 μg/mL (IgA ...
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bioRxiv - Molecular Biology 2020Quote: ... Native Mark Unstained Protein Ladder (Life technologies) was used as standard protein sizes ...
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bioRxiv - Cell Biology 2022Quote: ... 3-12% gradient native gels (Invitrogen, BN2011BX10) were used to separate proteins by blue native gel electrophoresis ...
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bioRxiv - Biophysics 2023Quote: ... The subsequent supernatant was mixed with native PAGE buffer and glycerol and loaded onto precast native PAGE gels (Invitrogen). ATP synthase dimerization state was probed using an anti-ATP synthase primary antibody (Rak & Tzagoloff ...
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bioRxiv - Biochemistry 2024Quote: The samples for blue native PAGE (BN-PAGE) analysis were prepared using a Native PAGE Sample Prep Kit (Invitrogen). For BN-PAGE ...
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bioRxiv - Immunology 2020Quote: ... At the end of the incubation the samples were separated under native conditions on BN-PAGE (Native PAGE BisTris Gels System 4–16%, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... At the end of the incubation the samples were separated under native conditions on BN-PAGE (Native PAGE BisTris Gels System 4–16%, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... These prepared samples were loaded on 3–12% Bis-Tris Native PAGE gels and electrophoresed using 1×Native PAGE Running buffer system (Invitrogen). The cathode buffer included 1×Cathode Buffer Additive (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... These prepared samples were loaded onto 3–12% Bis-Tris Native PAGE gels and electrophoresed using a 1× native PAGE running buffer system (Invitrogen). The cathode buffer included 1× cathode buffer additive (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was performed with Low pH IHC Antigen Retrieval Solution (Invitrogen) in a pressure cooker set to 100°C for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 2.5 μl of native additive G250 5% (Invitrogen) was added to the supernatant and 100 μg of protein was loaded onto a 3-12% native gel (NativePAGE™ (Invitrogen)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Native TBE 6% PAGE gels (Thermo Fisher # EC62652BOX) were run with 1X Hi-Density TBE Sample Buffer (Thermo Fisher # LC6678 ...
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bioRxiv - Immunology 2020Quote: ... pneumophila were then washed as before and examined using the Attune Flow Cytometer (Applied Biosystems, Foster City, California, USA). Flow cytometric data were analysed using the FlowJo software.
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bioRxiv - Microbiology 2023Quote: ... pneumophila followed by secondary probing with goat anti-rabbit Alexa Fluor 594 (Invitrogen, Cat# 111-585-144, 1:500) and donkey anti-mouse Alexa Fluor 488 (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... First dimensional BN-PAGE was run on NativePAGE™ 3-12% Bis-Tris gels under native conditions at 150V for 30 minutes with 1x native cathode buffer (Invitrogen) followed by 90 minutes at 250V in 0.1x native cathode buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Prepared samples were run on NativePAGE™ 3-12% Bis-Tris gels under native conditions at 150V for 30 minutes with 1x native cathode buffer (Invitrogen) followed by 90 minutes at 250V in 0.1x native cathode buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval was performed using IHC Antigen Retrieval Solution (ThermoFisher, 00-4955-58) in a steamer for 40Dminutes ...
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bioRxiv - Microbiology 2021Quote: ... The instrument was calibrated with Native Mark standards (ThermoFisher) at room temperature before use ...
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bioRxiv - Biochemistry 2019Quote: ... Lysate was added to 4x native sample buffer (Invitrogen), run on a NativePAGE gel (Invitrogen) ...
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bioRxiv - Zoology 2021Quote: ... then mixed with native-PAGE loading buffer (Invitrogen, USA) and separated by 3-16% BN-PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... blue native PAGE (NativePAGE, 4-16%, Bis-Tris, Invitrogen) and SDS-PAGE (NuPAGE™ 4 to 12% ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was added to 4x native sample buffer (Invitrogen), run on a NativePAGE gel (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... and yeast extract (Thermo Scientific™ Yeast Extract, Ultrapure, AAJ23547A1). BHI medium was made using the premixed powder (BD™ Bacto™ Dehydrated Culture Media ...
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bioRxiv - Physiology 2022Quote: ... Targeted antigen retrieval was performed by boiling sections in 1x IHC antigen retrieval solution (Invitrogen) for 15-20 mins ...
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bioRxiv - Biochemistry 2021Quote: ... The gel was run overnight in Native PAGE buffers (Invitrogen). The gel for Coomassie blue staining was run in dark blue buffer until the dye front reached 1/3 of the gel (150V ...
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bioRxiv - Molecular Biology 2022Quote: ... and without boiling loaded onto Native-PAGE gels (NativePAGE, Invitrogen). MES-buffer containing 0.1 % SDS (Invitrogen ...