Labshake search
Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Lassa Fever Virus GP1 Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: Genomic RNAs of PRRSV (porcine reproductive and respiratory syndrome virus) and CSFV (classic swine fever virus) were extracted following the TRIzol method (TRIzol reagent, Invitrogen, California, USA). Extracted RNA of PRRSV was examined using VDx PRRSv qRT-PCR (NA/EU dual ...
-
bioRxiv - Microbiology 2019Quote: ... 0.35μg of each of 10 reovirus genes and 0.8μg C3P3 (T7 RNA Polymerase linked with (G4S)4 to NP8686 African Swine Fever Virus capping enzyme) were diluted in 50μl Opti-MEM (31985070, ThermoFisher Scientific). 11μl TransIT®-LT1 (MIR2300 ...
-
bioRxiv - Microbiology 2021Quote: ... The Yellow fever NS5 protein was codon-optimised using the algorithms provided by Invitrogen (Thermo Fisher, USA), and a FLAG-tag encoding sequence was added to the carboxyl-terminus ...
-
bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
-
bioRxiv - Plant Biology 2021Quote: ... the 5’LTR sequence of GP1 (AT2TE23670) was cloned into the donor vector pDONR207 (Invitrogen) using primers PHG121 and PHG124 ...
-
bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
-
bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
-
bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 647-conjugated anti-Fc Fab (Surface protein and CYpHER quantitation, ThermoFisher Zenon Labeling Kits, mouse IgG2a for anti-EGFR 199.12 quantitation [ThermoFisher Z25108] ...
-
bioRxiv - Neuroscience 2023Quote: ... was recombinantly expressed as an N-terminal Fc-fusion protein (Fc-hTfR1) in Expi293 suspension cells and purified by gravity flow with PierceTM Protein A Agarose (Thermo Fisher Scientific). HFCII probes were loaded with Fc-hTfR1 by thresholding to a 1 nm shift ...
-
bioRxiv - Immunology 2021Quote: ... then Fc blocked with normal mouse serum (Thermo Fisher) and TruStain FcX αmouse CD16/32 (Biolegend ...
-
bioRxiv - Immunology 2019Quote: ... PBS- or mouse IgG Fc isotype (ThermoFisher Scientific, 31205) – treated mice were compared and used as negative controls ...
-
bioRxiv - Microbiology 2020Quote: ... GP was detected by western blotting with an anti-GP1 polyclonal rabbit serum described previously (22) followed by an Alexa-Fluor 680 dye conjugated anti-rabbit secondary antibody (ThermoFisher). Blots were imaged using the LI-COR Fc fluorescence imager (LI-COR ...
-
bioRxiv - Biophysics 2021Quote: ... and both HRP-conjugated anti-mouse IgG Fc and anti-human IgG Fc were purchased from Invitrogen™ (Waltham ...
-
bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked by incubating cells with 1:50 anti- mouse CD16/CD32 (Fc block, Invitrogen) for 10 minutes at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... or IgG1 Fc (human) protein (Thermo Fisher Scientific, Rockford, IL) was added to a final concentration of 10 mg/mL and samples were incubated with gentle mixing for 2 hours at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...
-
bioRxiv - Immunology 2021Quote: ... then Fc receptors blocked with normal mouse serum (Thermo Fisher) and TruStain FcX αmouse CD16/32 (Biolegend ...
-
bioRxiv - Bioengineering 2021Quote: ... IgG Fc-HRP goat-anti-mouse secondary antibody (Invitrogen A16084) was then added at a 1:10,000 dilution (in 1%BSA ...
-
bioRxiv - Immunology 2022Quote: ... Then anti-Mouse IgG Fc Secondary antibody (Invitrogen Cat#31437) was diluted in HRP dilution buffer (Sigma Cat#18995 ...
-
bioRxiv - Biochemistry 2021Quote: ... ACE2-Fc was purified over Protein A Agarose (Thermo Fisher; 15918014). Once the Protein A Agarose was equilibrated in PBS buffer ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc (18-615) protein expressed in Expi293F (Life Technologies) cells was chemically biotinylated using EZ-link Sulfo-NHS-Biotin (A39256 ...
-
bioRxiv - Immunology 2023Quote: ... KIR-Fc were conjugated to protein-A Alexa 647 (Invitrogen, #P21462) overnight at 1:4 (molar ...
-
bioRxiv - Cell Biology 2019Quote: ... goat anti-mouse Fc-HRP(1:3000, Thermo Fisher Scientific, #31437).
-
bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins fused with Fc were captured using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), eluted with pH 3.0 glycine (100 mM in H2O) ...
-
bioRxiv - Cancer Biology 2019Quote: ... These α1-α2 phage phage libraries were captured with either biotinylated Fc-iNKG2D.YA or Fc-iNKG2D.AF protein (EZ-LinkTM NHS-Biotin Kit, Thermo Fisher #20217) and enriched by cycling through four rounds of selection with increasing concentrations of non-biotinylated Fc-wtNKG2D competitor ...
-
bioRxiv - Immunology 2019Quote: ... KIR2DL1*001-Fc, and KIR2DS4*001-Fc (R&D, 1844-KR, 1847-KR) were conjugated to protein-A Alexa Flour 647 (Invitrogen) by overnight incubation at 9:1 (molar ...
-
bioRxiv - Bioengineering 2021Quote: ... Laminin Mouse Protein (ThermoFisher) was added to the solution containing calcium and cells to a final concentration of 1 mg/mL ...
-
bioRxiv - Microbiology 2022Quote: ... The protein concentration of the purified virus preparations was determined using a BCA protein assay kit (Thermo Fisher Scientific) prior to the addition of lysis buffer ...
-
bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A Virus M1 (1:1,000; clone: GA2B; Cat#: MA1-80736; Invitrogen), mouse anti-Influenza A virus M2 (1:1,000 ...
-
bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A virus M2 (1:1,000; clone: 14C2; Cat#: MA1-082; Invitrogen), mouse anti-GAPDH (1:1,000 ...
-
bioRxiv - Microbiology 2023Quote: ... mono-Fc was removed using Pierce Protein A agarose beads (Thermo Scientific). The flow-through containing PvDBP-RII was then mixed with DB1 or DB9 Fab (1.5:1 Fab to protein ratio ...
-
bioRxiv - Immunology 2020Quote: ... at a concentration to give 100 to 250 foci per well in the virus only control wells was mixed 50:50 in 1% FCS MEM with 1 x antibiotic/antimycotic (Gibco, 15240-062) with serum doubling dilutions from 1:20 to 1:640 (or higher dependent on antibody levels ...
-
bioRxiv - Immunology 2024Quote: ... the anti-mouse IgG Fc cross-adsorbed secondary antibody (PE, Thermo Fisher) was added to detect the anti-rhesus antibodies with fluorescence ...
-
bioRxiv - Genomics 2020Quote: ... cells were washed twice in FC buffer and anti-mouse Fc Alexa Fluor 647-conjugated secondary antibody (Thermo Scientific, A-21236) was added at 1:500 dilution ...
-
bioRxiv - Cell Biology 2023Quote: Cells in the exo-erythrocytic development assays were subsequently stained with a primary antibody against Plasmodium berghei HSP70 (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and an anti-mouse Alexa Fluor 488 (1:300 diluted in 10% FCS/ PBS; Life technologies). Hoechst 33343 was added after for 5 min at RT and the cells washed twice in 1% FCS/ PBS the assays before being mounted ...
-
bioRxiv - Cell Biology 2023Quote: Cells in the invasion assay were stained subsequently for 1 h at 37°C with primary mouse αCSP antibodies (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and anti-mouse Alexa Fluor 488 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies). Following two washing steps with 1% FCS/PBS the cells were permeabilized by addition of ice-cold methanol for 10 min at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... Subsequently another round of blocking with 10% FBS/ PBS at 37°C or at 4°C overnight was followed by a second round of subsequent incubation with αCSP (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and secondary anti-mouse Alexa Fluor 546 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies) for 1 h at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins from virus-infected cells were immunoprecipitated using protein G magnetic Dynabeads according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Briefly ...
-
bioRxiv - Microbiology 2022Quote: ... Nb fused with Fc was purified using Protein G (cat.# 20399, Thermo Scientific).
-
bioRxiv - Cancer Biology 2020Quote: ... The M25 scFv-Fc was immobilized onto protein A beads (Thermo Fisher Scientific) and cross-linked by dimethyl pimelimidate dihydrochloride (DMP ...
-
bioRxiv - Cancer Biology 2022Quote: ... VH-Fc proteins were expressed in the Expi293 expression system (Thermo Fisher Scientific) and purified with protein A resin (GenScript) ...
-
bioRxiv - Physiology 2023Quote: ... Fc-FGF-21CT was purified using protein A sepharose 4B (Thermo Fisher Scientific) and dialyzed against PBS ...
-
bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 647-conjugated anti-Fc Fab (Surface protein and CYpHER quantitation, ThermoFisher Zenon Labeling Kits ...
-
bioRxiv - Neuroscience 2022Quote: ... Brain leukocytes were washed and blocked with mouse Fc Block (ThermoFisher, clone 93) prior to staining with primary antibody-conjugated flourophores ...
-
bioRxiv - Microbiology 2019Quote: ... CR-Fc or DC-SIGN-Fc (Thermofisher) in binding buffer (BB ...
-
bioRxiv - Immunology 2023Quote: ... Media containing virus particles was first concentrated to 20x using 100K MWCO protein concentrator columns (Thermofisher). Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887 ...
-
bioRxiv - Immunology 2023Quote: ... Clec12a-Fc or Fc only fusion proteins were isolated with Pierce™ Classic Magnetic IP/Co-IP Kit (Thermo Fisher Scientific, Catalog number: 88804), quantified with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific Catalog number ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fab and Fc regions were separated using a Nab Protein A column (Thermo Scientific). Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off ...
-
bioRxiv - Cell Biology 2021Quote: ... recombinant hACE2-Fc protein was first biotinylated using EZ-Link Sulfo-NHS-Biotin (ThermoFisher) as the instruction described ...