Labshake search
Citations for Thermo Fisher :
1 - 50 of 5838 citations for KRAS Human G12S His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... variant allele frequencies were determined by KRAS G12 and G13 mutation specific assays (Thermo Fisher Scientific) for patients M2 ...
-
bioRxiv - Cancer Biology 2020Quote: ... retroviral particles were generated by co-transfecting 1.25 μg pBABE-puromycin retroviral vectors expressing WT KRAS or each KRAS mutant with 1.25 μg/μl PCL10A pack vector using 6.25 μg Lipofectamine 2000 (ThermoFisher Scientific) into HEK293T cells seeded in a 6 cm cell culture plate per the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... stably expressing activated KRAS (HPDE-KRAS) and wild-type HPDE (HPDE-WT) were cultured in RPMI-1640 medium (Gibco, ThermoFisher Scientific) supplemented with 1% Penicillin-Streptomycin (Gibco) ...
-
bioRxiv - Systems Biology 2022Quote: The bacterial transformation of the plasmids for exogenous expression of FLAG-KRAS (pMDS-TetOn3G-kozak-FLAG-KRAS WT/mutants) was performed using the One-Shot™ Stbl3TM (Invitrogen, C737303) chemically competent bacterial cells to replicate each plasmid following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: Human pancreatic ductal epithelial cells (HPDE) stably expressing activated KRAS (HPDE-KRAS) and wild-type HPDE (HPDE-WT) were cultured in RPMI-1640 medium (Gibco, ThermoFisher Scientific) supplemented with 1% Penicillin-Streptomycin (Gibco) ...
-
bioRxiv - Biochemistry 2023Quote: ... anti-KRAS (1:1000; 11H35L14, Thermo Fisher Scientific, Oberhausen, Germany). Secondary antibodies employed were from LI-COR (Bad Homburg ...
-
bioRxiv - Microbiology 2020Quote: ... All human MARCH cDNAs were cloned into pCDNA3.1/myc-His A (Invitrogen) and were then subcloned into pBJ5 ...
-
bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and anti-KRAS (catalog # 41-570-0; Fisher Scientific, Hampton, NH, USA) at 1:100 dilution ...
-
bioRxiv - Microbiology 2021Quote: ... before staining with lead citrate and examined using a transmission electron microscope (Tecnai Spirit G12; Thermo Fisher Scientific/FEI, Eindhoven, Netherlands) at 120 kV ...
-
bioRxiv - Neuroscience 2023Quote: ... Full-length human LRRC4B was subcloned and inserted into the pcDNA6-V5-His vector (Invitrogen). FAM19A5 (NM_001252310.1 ...
-
bioRxiv - Neuroscience 2024Quote: ... After blocking supernatant samples from different groups and standards (recombinant human-TREM2-His; Life Technologies) were incubated for 2 h at room temperature (RT ...
-
bioRxiv - Synthetic Biology 2019Quote: ... TaqMan probes for KRAS and GFP were utilized with FAM dye label (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
-
bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
-
bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
-
bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
-
bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
-
bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
-
bioRxiv - Genomics 2022Quote: H358 lung adenocarcinoma cell lines harboring a KRAS(G12C) mutation were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (Sigma ...
-
bioRxiv - Genomics 2023Quote: H358 lung cancer cell lines containing the KRAS(G12C) mutation were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (Sigma ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
-
bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
-
bioRxiv - Microbiology 2021Quote: ... 10% HI FBS (Gibco), 1X ultraglutamine (Lonza) ...
-
bioRxiv - Microbiology 2021Quote: ... His (ThermoFisher, MA1-21315), FLAG (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... HI FBS (2273356P, Gibco). HEPES (54457 ...
-
bioRxiv - Immunology 2020Quote: ... or anti-His (Invitrogen) for recombinant ZVrecE80 antigen in 0.1M carbonate buffer pH 9.6 ...
-
bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
-
bioRxiv - Microbiology 2022Quote: ... anti-penta HIS (Invitrogen) and anti-HSP70 (26 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
-
bioRxiv - Cancer Biology 2024Quote: ... KRAS expression was validated in HEK293T cells via Western Blot after transfection with PEI (Thermo Fisher, BMS1003-A). The ASPS model was generated in a collaborative project with Priya Chudasama on “Immunogenomics characterization of alveolar soft part sarcoma”.
-
bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
-
bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
-
bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
-
bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
-
bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
-
bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
-
bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
-
bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
-
bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
-
bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
-
bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
-
bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10% HI FBS (Gibco A31604), and pen/strep and seeded into 96-well plates 24 hr prior to treatment ...