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Citations for Thermo Fisher :
1 - 50 of 9866 citations for Ion Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... Pierce strong ion exchange columns (ThermoFisher) were used following supplier’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... full volume media exchange to neurobasal media (Thermo Fisher, Cat # 21103049) was performed ...
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bioRxiv - Cancer Biology 2020Quote: ... the peptide pool was fractionated by Pierce Strong Ion Exchange Spin Columns (ThermoFisher). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... and the sample was loaded on the DNAPac PA200 ion exchange column (Thermo Fisher). Buffer A (2 mM Tris (pH 8.0) ...
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bioRxiv - Biophysics 2021Quote: ... The sample was loaded onto a 10-mL POROS HQ ion exchange column (Applied Biosystems) equilibrated with buffer B (60 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Ion exchange TBC-DEG purified fractions were then immediately concentrated using Amicon concentrators (Fisher Scientific) and then loaded on a HiLoad 16/600 Superdex-200 gel filtration column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Ion exchange resin was recovered by filtration and washed successively with distilled H2O (Fisher Scientific, UK) at 60°C with two volumes of water and 10 volumes of 1 M NaCl at r.t ...
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bioRxiv - Biochemistry 2021Quote: ... The Strep (II) elution fractions were further purified via ion exchange chromatography (Poros HQ 50 μm, Life Technologies) and subjected to size-exclusion chromatography in a buffer containing 50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was additionally purified by ion-exchange chromatography on a “POROS HQ/20” 4.6×100 mm column (Applied Biosystems) using 0-20%B gradient over 12 column volumes at 0.5 ml/min flow rate using 20 mM Tris-HCl buffer (pH 8.9 at 25 °C ...
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bioRxiv - Systems Biology 2023Quote: ... The bottom tips were packed sequentially with three plugs of C18 membrane (3 M Empore) and mixed-mode ion exchange beads (SCX:SAX = 1:1, Applied Biosystems). The quantity of C18 membrane and mixed-mode ion exchange beads was adjusted based on the protein amount.
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bioRxiv - Cell Biology 2022Quote: ... The Strep (II) and Ni-NTA elution fractions were further purified via ion exchange chromatography (Poros HQ 50 μm, Life Technologies) and subjected to size-exclusion chromatography in a buffer containing 50 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... a 75% media exchange (150uL) was performed in all wells with NB/B27+ media (B-27 Plus Neuronal system kit (Life technologies A3653401). On D5 ...
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bioRxiv - Molecular Biology 2020Quote: An untargeted analysis workflow was applied using a reagent-free ion exchange chromatography system with eluent generation (RFIC-EG) hyphenated with a high-field Orbitrap platform (Thermo Scientific, Bremen, Germany). The autosampler and columns were thermostated at 8 °C and 40°C ...
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bioRxiv - Biochemistry 2022Quote: ... Before metabolite assay to each sample 140 pmoles of the appropriate standard was added and then selected metabolites were separated and quantitively analyzed with the use of an ion-exchange chromatography system (Dionex ICS-3000 chromatograph, Thermo Fisher Scientific Inc.) coupled with Waters ZQ mass spectrometer (Waters Corporation) ...
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Single-cell RNA sequencing reveals distinct tumor microenvironmental patterns in lung adenocarcinomabioRxiv - Cancer Biology 2020Quote: ... an Ion 530 chip and the Ion Chef/Ion S5 XL System (ThermoFisher).
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bioRxiv - Immunology 2024Quote: ... and Ion 510 & Ion 520 & Ion 530 Kit - Chef on the Ion Chef platform (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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Single-cell RNA sequencing reveals distinct tumor microenvironmental patterns in lung adenocarcinomabioRxiv - Cancer Biology 2020Quote: ... and the Ion Chef/Ion S5 XL System (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... and Ion 318™ v2 Ion Chip (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... the cells were subjected to antibiotic selection by exchange into adherent culture media containing 2 μg/mL Blasticidin (Gibco) for 10 days with regular exchange into fresh selective media and passaging to prevent cells from achieving full confluency ...
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bioRxiv - Microbiology 2023Quote: ... Cells then underwent a media exchange in which half the plates received the normal DMEM while the other half received glucose-free DMEM (Gibco). To perform the media exchange ...
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bioRxiv - Immunology 2021Quote: ... Buffer exchange to PBS (Gibco) was performed using 30 kDa Amicon Ultra-15 columns (Millipore ...
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bioRxiv - Genomics 2020Quote: ... Libraries were then pooled and diluted to 50 pM prior to loading onto Ion 530™ or 540™ Chips using the Ion Chef Instrument and Ion 530™ or Ion 540™ Kit (Thermo Fisher Scientific). Following template preparation ...
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bioRxiv - Systems Biology 2019Quote: ... The Ion 520 & Ion 530 Kit-Chef (Life Technologies, California, USA) was employed for template preparation and sequencing according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... via a nanoelectrospray ion source (Nanospray Flex Ion Source, Thermo Scientific). The MS2 ion count target was set to 1.5 x 104 ...
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bioRxiv - Microbiology 2021Quote: ... The amplified PCR products were gel purified and loaded on the ion 540 or 520 Chip for next-generation sequencing using the Ion Chef and Ion S5 sequencing system (ThermoFisher) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... on Ion 314 chips with Ion PGM Sequencing 400 Kit (Life Technologies), according to the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... Ion 530 chips were prepared using Ion Chef System (Thermo Fisher Scientific) and NGS reactions were run on an Ion GeneStudio S5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Template preparation of the multiplexed libraries was performed on the Ion Chef system with subsequent sequencing on the Ion S5 XL sequencer (Ion Torrent, Thermo Fisher). For each sequenced RNA sample ...
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bioRxiv - Microbiology 2020Quote: ... samples were sequenced on an Ion PGM Sequencer with Ion PGM Hi-Q View Sequence Solutions (Ion Torrent Life Technologies, USA).
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bioRxiv - Genomics 2020Quote: ... PMPs were harvested from suspension during medium exchange and plated in microglia differentiation media over 7-14 days to produce microglia like cell monocultures (Neurobasal (Life Technologies 21103049) supplemented with Gem21 NeuroPlex without Vitamin A (GeminiBio ...
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bioRxiv - Molecular Biology 2020Quote: ... and Ion PI HiQ sequencing kit using Ion PI V3 chips (Life Technologies) and a Proton Sequencer (Thermofisher) ...
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bioRxiv - Genomics 2021Quote: ... Shotgun metagenomics sequencing was performed on an Ion Proton sequencer (Ion Torrent, Thermofisher). Eight to ten DNA libraries with different barcodes were pooled together at a final concentration of 250 pM ...
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bioRxiv - Neuroscience 2022Quote: ... via an Ion Max ion source with a HESI II probe (Thermo Scientific). A Sequant ZIC-pHILIC column (2.1 mm i.d ...
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bioRxiv - Cell Biology 2022Quote: ... via an Ion Max ion source with a HESI II probe (Thermo Scientific). The mobile phase consisted of buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... via an Ion Max ion source with a HESI II probe (Thermo Scientific). The mobile phase consisted of buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... via an Ion Max ion source with a HESI II probe (Thermo Scientific). The mobile phase consisted of buffer A ...
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bioRxiv - Synthetic Biology 2023Quote: ... following equilibration of the anion-exchange column (0.5 mL strong anion-exchange spin column; 90010, Thermo Scientific) with purification buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... Sequencing was conducted on the Ion Torrent PGM sequencer using the Ion PGM Sequencing 200 Kit v2 and the Ion 314 Chip v2 (Thermo Fisher Scientific) according to the manuals ...
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bioRxiv - Molecular Biology 2022Quote: ... The sequencing was performed on Ion 318 v2 chip and Ion Torrent PGM machine employing the Ion PGMTM Hi-QTM View Sequencing kit (Life Technologies, USA). All procedures were carried out according to manufacturer’s instructions and each run was expected to produce approximately 150,000 reads per sample ...
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bioRxiv - Immunology 2022Quote: ... The libraries were sequenced on Ion Proton using an Ion PI Hi-Q Sequencing 200 kit and Ion PI Chip v3 (Thermo Fisher Scientific). The FASTQ files were generated using AmpliSeqRNA plug-in v5.2.0.3 in the Torrent Suite Software v5.2.2 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... dorsal forebrain organoids were transferred from 96- to 24-well low adhesion plates and moved to an orbital shaker rotating at 53 rpm (2.5 cm throw) with media exchange to CDMII (DMEM/F12-based medium containing 1X Glutamax (Thermofisher Scientific, Cat. no. 31331093), 1X N2 Supplement (Thermofisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... loaded on an Ion 540™ chip using the Ion ChefTM instrument and sequenced on an Ion S5™ instrument (Thermo Fisher Scientific). QC was manually performed for each sample based on the following metrics ...
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bioRxiv - Molecular Biology 2020Quote: ... HTS was performed using Ion Torrent™ Personal Genome Machine™ (ION-PGM) using Ion PGM HiQ View sequencing kit (Life technologies, A30044) and Ion 316v2 chips (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... The resulting libraries were multiplexed and sequenced with Ion Proton technology and Ion Hi-Q chemistry (Ion Torrent, Thermo Fisher Scientific, Vienna, Austria). CUTADAPT 4.0 was used to trim sequencing reads of low-quality bases and poly-A tails with the options -q 20 ...
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bioRxiv - Microbiology 2019Quote: ... The Ion OneTouch 2 system using the Ion PGM Template OT200 Kit (Life Technologies) was used for template preparation which includes the steps of emulsification ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using an Ion 318 chip and Ion PGM Sequencing 200 Kit v2 (Life Technologies). For de novo phages genome assembler ...
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bioRxiv - Systems Biology 2019Quote: ... analyser using Ion 318 chips and Ion PGM sequencing 400 Kit v2 (Life Technologies) in accordance with the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... Major ion concentrations were determined by ion chromatography (Thermo Scientific Dionex capillary ICS-5000) (Hawkings et al. ...
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bioRxiv - Microbiology 2020Quote: ... Positively charged ions were generated by a Nanospray Flex Ion Source (Thermo Fisher Scientific) using a stainless steel emitter with 2.2 kV spray voltage ...
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bioRxiv - Cancer Biology 2022Quote: ... we used Ion 520/530-OT2 kit (Ion Torrent, Life Technologies, Grand Island, NY) with the Ion 530 Chip (Ion Torrent) ...