Labshake search
Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Human Immunodeficiency Virus Gag Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
-
bioRxiv - Immunology 2020Quote: ... HIV Gag or CMV UL40 (Thermofisher). After washing ...
-
bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 pseudoviruses were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective spike protein into 293T cells (ATCC) ...
-
bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
-
bioRxiv - Microbiology 2019Quote: ... Gag was immunoprecipitated by incubating the cell lysates with HIV-Ig bound to DynabeadsTM Protein G (Invitrogen) for 1 hour on a rotating shaker at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... phCMV-VSVG and HIV gag-pol in the presence of Turbofect (Life Technologies) according to the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... by intracellular staining and labelled for HIV gag mRNA using the PrimeFlow RNA assay (ThermoFisher) before acquisition on a flow cytometer (FACS Fortessa ...
-
bioRxiv - Physiology 2020Quote: ... 600 nM of HIV gag reverse primer and 5 U of Platinum Taq DNA polymerase (Life Technologies; USA). In the 2nd round PCR 5’ LTR region of HIV-1 was amplified using 1 μl of 1st round PCR amplicon as a template ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
-
bioRxiv - Microbiology 2023Quote: ... and stained with FITC-conjugated anti-SIV Gag antibody (clone 552F12) in Perm/Fix Medium B (Invitrogen). After washing ...
-
bioRxiv - Cell Biology 2022Quote: ... were combined with 0.36 μg of recombinant human protein CDK1/Cyclin B (Thermo Fisher, PV3292), 1x Kinase Buffer (Cell Signaling ...
-
bioRxiv - Microbiology 2020Quote: ... N-term B virus mut) using a Neon® Transfection System (Invitrogen) under the recommended electroporation condition (1350 V ...
-
bioRxiv - Microbiology 2020Quote: ... just-subconfluent T150 flasks were transfected with 8.75 μg of HIV-1 YU2 or HIV-1 YU2 lacking Vpr (HIV-1 YU2 ΔVpr) and 30 μl Fugene 6 in 500 μl Optimem (Thermofisher Scientific). To make VSV-G pseudotyped HIV-1 GFP ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2022Quote: ... B-lymphocytes were enriched using Dynabeads Untouched Human B Cells Kit (Invitrogen). Each sample was loaded into two lanes (i.e ...
-
bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
-
bioRxiv - Microbiology 2020Quote: ... concentrated HIV-1 virions were lysed by Trizol (Invitrogen) and RNA was purified by phenolic-chloroform sedimentation and isopropanol precipitation ...
-
bioRxiv - Synthetic Biology 2022Quote: ... HIV gp120 (NIH HIV Reagent Program, Cat. #13354) was immobilized at 1 µg/mL on 96-well assay plates (Nunc Maxisorp ...
-
bioRxiv - Molecular Biology 2023Quote: ... Abelson virus-transformed pre-B cells were maintained in DMEM (Thermo Fisher, 11960-077) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2022Quote: ... and interacting proteins were co-immunoprecipitated with 5 µg of anti-gag antibody per mg of total proteins using Dynabeads protein G (Invitrogen). Proteins were solubilized in Laemmli buffer and separated by SDS–PAGE ...
-
bioRxiv - Physiology 2023Quote: ... Human microRNA A and B (Life Technologies, Australia). Collectively ...
-
bioRxiv - Molecular Biology 2021Quote: Abelson virus-transformed pre-B cell lines were maintained in DMEM (Thermo Fisher #11960-077) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Immunology 2023Quote: All HIV-1 mAbs were expressed in Expi293F cells (Thermo Fisher) as described previously22 ...
-
bioRxiv - Neuroscience 2020Quote: Human MAO B cDNA was obtained from Invitrogen (IOH55406), AcGFP was obtained from Clontech (632489) ...
-
bioRxiv - Physiology 2023Quote: ... Human Pool A and B v3.0 (Life Technologies, Australia) as per manufacturer’s protocol ...
-
bioRxiv - Genetics 2022Quote: ... and B sample (Human Brain Reference RNA, ThermoFisher, Inc.)13 ...
-
bioRxiv - Cancer Biology 2022Quote: ... human antibody HLA-A,B,C (1:100, W6/32, #17-9983-42) (Thermo Fisher). To distinguish live/dead cells ...
-
bioRxiv - Biophysics 2020Quote: ... B-PER Bacterial Protein Extraction Reagent (ThermoFisher) was used to lyse the cells in the presence of protease inhibitor ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were infected with virus particles and selected with hygromycin B (200 µg/ml, Invitrogen). For single clone selection ...
-
bioRxiv - Microbiology 2022Quote: ... The clade I nosZ gene was amplified with PowerUp SYBR Green master mix (Applied Biosystems, Foster City, CA), to which 3 mM MgCl2 was added ...
-
bioRxiv - Immunology 2020Quote: Recombinant HIV-1 Env gp120 was expressed in Freestyle 293 cells (ThermoFisher) by transient transfection ...
-
bioRxiv - Biochemistry 2022Quote: ... The ORF of human MYBL2/B-Myb isoform 1 (NM_002466.4) was cloned into pcDNA3.1+ (ThermoFisher Scientific) and fused with an N-terminal Flag tag ...
-
bioRxiv - Cell Biology 2024Quote: Caspase-1 – CCA CUG AAA GAG UGA CUU Utt (Thermo Fisher; siRNA ID s2408)
-
bioRxiv - Systems Biology 2023Quote: ... The full-length proteins and their N-terminal proteoforms were amplified from the corresponding pMET7-GAG-sp1-FL or pMET7-GAG-sp1-PR plasmids of each gene using the AccuPrimeTM pfx DNA polymerase (Invitrogen) and ORF-specific primers supplemented with attB sites ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were extracted using B-PER (ThermoFisher Scientific) from overnight cultures of bacteria growing in LB media supplemented with 100 μg/mL ampicillin and 0.0016% L-arabinose and then tested for fluorescence and Ca2+ response in 384-well plates ...
-
bioRxiv - Pathology 2019Quote: ... CSF-1 Recombinant Human Protein (25ng/mL, Thermo Fisher Scientific PHC9504), and Recombinant Human TGF-β1 (50ng/mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... rabbit monoclonal anti-human p53 protein SP5 (1:200) (Invitrogen, USA) and mouse monoclonal anti-p63 antibody (1:500 ...
-
bioRxiv - Immunology 2022Quote: ... When HIV was reactivated using Dynabeads Human T activator CD3/CD28 for T cell expansion and activation (ThermoFisher Scientific), beads were magnetically removed the next day ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 × B-27 (Gibco), 1.25 mM N-acetylcysteine (Sigma) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1× B-27 (GIBCO), 1× N2 (GIBCO) ...
-
bioRxiv - Microbiology 2019Quote: ... 1 % amphotericin B (Gibco), and 20 ng/mL macrophage colony-stimulating factor (mCSF ...
-
bioRxiv - Microbiology 2020Quote: ... 1% amphotericin B (Gibco)] ...
-
bioRxiv - Microbiology 2023Quote: ... 1× B-27 (Gibco), 1× N-2 (Gibco) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1% B-27 (Gibco), 1% GlutaMAX (Gibco) ...
-
bioRxiv - Microbiology 2020Quote: 293T cells were transfected with HIV-1 proviral DNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: Primary human B cells were cultured in IMDM medium (Life Technologies) containing 10% heat-inactivated fetal calf serum ...
-
bioRxiv - Microbiology 2024Quote: ... were cultured in human B+ erythrocytes in RPMI 1640 medium (GIBCO) supplemented with 10% human type AB-positive plasma ...
-
bioRxiv - Immunology 2023Quote: ... HIV-1JRFL or HIV-1AD8 infected primary CD4+ T cells were stained with viability (AquaVivid; Thermo Fisher Scientific) and cellular (cell proliferation dye eFluor670 ...
-
bioRxiv - Microbiology 2019Quote: ... the membrane was probed with HIV-Ig as the primary antibody and corresponding secondary antibody conjugated to horseradish peroxidase (goat anti-human IgG, Invitrogen). Gag-specific bands were detected after incubation with SuperSignal West Pico chemiluminescent substrate (Pierce ...