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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade AE CM240 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
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bioRxiv - Immunology 2020Quote: Recombinant HIV-1 Env gp120 was expressed in Freestyle 293 cells (ThermoFisher) by transient transfection ...
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bioRxiv - Synthetic Biology 2022Quote: ... HIV gp120 (NIH HIV Reagent Program, Cat. #13354) was immobilized at 1 µg/mL on 96-well assay plates (Nunc Maxisorp ...
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bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 pseudoviruses were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective spike protein into 293T cells (ATCC) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Y-Ae (clone eBioY-Ae; ThermoFisher Scientific), Thy1.2 (clone 30-H12 ...
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Standard Western blotting procedures were used with the following antibodies: HIV-1 gp120 (NIH AIDS Reagent program catalog no. 288) and 6x-His Tag (ThermoFisher catalog no. MA1-21315-HRP).
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bioRxiv - Microbiology 2023Quote: ... The cell and virus lysates were Western blotted and probed with a goat polyclonal anti-gp120 antibody (Invitrogen), the 4E10 anti-gp41 antibody (NIH HIV Reagent Program ...
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bioRxiv - Neuroscience 2023Quote: ... 2:1 ethanol/hexamethyldisilazane (HMDS, Thermo Scientific #A15139.AE)) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the proteins analyzed by Western blotting using 1:2,000 goat anti-gp120 polyclonal antibody (Thermo Fisher Scientific) and 1:2,000 HRP-conjugated rabbit anti-goat IgG (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Atto Ae-6050 Mini Gel chamber and Novex protein separation from Thermo Fisher, respectively ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
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bioRxiv - Molecular Biology 2022Quote: ... Sf9 cells (IPLB-Sf21-AE, Invitrogen, Germany) were propagated in serum free Sf-900 III medium (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Western blots were developed with 1:2,000 goat anti-gp120 polyclonal antibody (Invitrogen), 1:2,000 4E10 anti-gp41 antibody ...
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bioRxiv - Microbiology 2023Quote: ... Western blots were developed with 1:2500 goat anti-gp120 polyclonal Ab (Invitrogen), 1:2500 4E10 anti-gp41 Ab (Polymun) ...
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bioRxiv - Immunology 2021Quote: ... Expression of gp120 proteins occurred as previously described in 293F cells by transient transfection with 293Fectin (Invitrogen) and purification by metal affinity and Size Exclusion Chromatography (Alam et al. ...
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bioRxiv - Immunology 2022Quote: ... purified HIVJR-CSF gp120 protein was biotinylated using EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) and excess biotin was removed using a Zeba spin desalting column (Thermo Fisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 1 µl 0.5 M ethylenediaminetetraacetic acid (EDTA, Thermo Scientific, Ca. No. J15694.AE), and 1 µl 0.5 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2021Quote: ... boiled and analyzed by Western blotting with 1:2,000 goat anti-gp120 antibody (Invitrogen) and 1:3,000 HRP-conjugated rabbit anti-goat antibody (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... just-subconfluent T150 flasks were transfected with 8.75 μg of HIV-1 YU2 or HIV-1 YU2 lacking Vpr (HIV-1 YU2 ΔVpr) and 30 μl Fugene 6 in 500 μl Optimem (Thermofisher Scientific). To make VSV-G pseudotyped HIV-1 GFP ...
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bioRxiv - Genomics 2023Quote: ... AE-2 (cat. MA1-35346, Thermo Fisher Scientific). Antibodies were tested for binding to dsDNA using a mouse anti-dsDNA IgG ELISA kit per the manufacturer’s instructions (cat ...
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bioRxiv - Biophysics 2024Quote: ... and 0.2% Glutaraldehyde (Thermo Scientific Chemicals, A17876.AE) in HBSS on ice for 1 hour.
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bioRxiv - Immunology 2020Quote: ... purified gp120 and SOSIP proteins were covalently coupled to 30 mg of tosylactivated MyOne Dynabeads (Life Technologies Inc.) in coupling buffer [0.1 M NaBO4,1M(NH4)2SO4 ...
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bioRxiv - Immunology 2022Quote: ... we labeled the gp120-specific antibody (A32 mAb) with Allophycocyanin (APC) following the manufacturer instructions (Zenon Human IgG labeling kit, Invitrogen) and measured cell binding by flow cytometry ...
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bioRxiv - Microbiology 2020Quote: ... concentrated HIV-1 virions were lysed by Trizol (Invitrogen) and RNA was purified by phenolic-chloroform sedimentation and isopropanol precipitation ...
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bioRxiv - Microbiology 2023Quote: ... boiled and analyzed by Western blotting using 1:2000 goat anti-gp120 polyclonal antibody (ThermoFisher) and 1:2000 HRP-conjugated rabbit anti-goat IgG (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... with 0.5 M EDTA (Thermo Scientific Catalog #J15694-AE) and PEG600 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... and analyzed by Western blotting with 1:2,000 goat anti-gp120 polyclonal antibody (Thermo Fisher Scientific) and 1:2,000 HRP-conjugated rabbit anti-goat IgG (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2023Quote: Livers were homogenized in RIPA buffer (Thermo Scientific, J62524.AE) supplemented with protease inhibitors ...
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bioRxiv - Developmental Biology 2023Quote: ... 25 µl 10% IGEPAL (Cat# J61055-AE, Thermo Fisher Scientific) was added to lyse cells and vortexed briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies for Western blotting include goat anti-gp120 polyclonal antibody (ThermoFisher) and the 4E10 human anti-gp41 antibody (Polymun) ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies for Western blotting include goat anti-gp120 polyclonal antibody (ThermoFisher) and the 4E10 human anti-gp41 antibody (Polymun) ...
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bioRxiv - Immunology 2023Quote: Recombinant gp120s were produced via transient transfection using Freestyle 293F (ThermoFisher) or 293S GnT1- cells and 293Fectin using the same conditions as SOSIP gp140s ...
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bioRxiv - Immunology 2023Quote: All HIV-1 mAbs were expressed in Expi293F cells (Thermo Fisher) as described previously22 ...
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bioRxiv - Immunology 2023Quote: ... was reconstituted in dimethylsulfoxide (DMSO, Thermo Fisher Scientific, cat# J66650.AE) at a stock concentration of 5 mg/ml and stored at -20°C ...
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bioRxiv - Genomics 2023Quote: ... 4) 1M Tris-HCl pH 8.0 (J22638.AE, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... The clade I nosZ gene was amplified with PowerUp SYBR Green master mix (Applied Biosystems, Foster City, CA), to which 3 mM MgCl2 was added ...
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bioRxiv - Immunology 2020Quote: ... HIV Gag or CMV UL40 (Thermofisher). After washing ...
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bioRxiv - Pathology 2019Quote: ... CSF-1 Recombinant Human Protein (25ng/mL, Thermo Fisher Scientific PHC9504), and Recombinant Human TGF-β1 (50ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit monoclonal anti-human p53 protein SP5 (1:200) (Invitrogen, USA) and mouse monoclonal anti-p63 antibody (1:500 ...
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bioRxiv - Immunology 2022Quote: ... When HIV was reactivated using Dynabeads Human T activator CD3/CD28 for T cell expansion and activation (ThermoFisher Scientific), beads were magnetically removed the next day ...
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bioRxiv - Microbiology 2019Quote: ... Gag was immunoprecipitated by incubating the cell lysates with HIV-Ig bound to DynabeadsTM Protein G (Invitrogen) for 1 hour on a rotating shaker at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... Monomeric ConM gp120 was captured with C-Tag affinity column (Thermo Scientific, 2943072005), ConM gp120 F10 and SOSIP were captured with house-made PGT145 or CH01 columns ...
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bioRxiv - Microbiology 2020Quote: 293T cells were transfected with HIV-1 proviral DNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... HIV-1JRFL or HIV-1AD8 infected primary CD4+ T cells were stained with viability (AquaVivid; Thermo Fisher Scientific) and cellular (cell proliferation dye eFluor670 ...
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bioRxiv - Microbiology 2019Quote: ... the membrane was probed with HIV-Ig as the primary antibody and corresponding secondary antibody conjugated to horseradish peroxidase (goat anti-human IgG, Invitrogen). Gag-specific bands were detected after incubation with SuperSignal West Pico chemiluminescent substrate (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: ... Virus was then resuspended in 1:1 Opti-MEM (Gibco) - HBSS ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...