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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Herpes Simplex Virus 2 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and a panel of ProcartaPlex Human Simplex (Invitrogen) beads ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Immunology 2021Quote: ... G-CSF mouse or human ProcartaPlex Simplex Kit (ThermoFisher Scientific), G-CSF ELISA kit (R&D Systems) ...
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bioRxiv - Microbiology 2023Quote: ... combined with a Simplex IFNa assay (ThermoFisher EPX01A-26027-901). Samples were run on a Luminex Bio-Plex 200 system with BioPlex Manager 6.1.1 software ...
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bioRxiv - Molecular Biology 2020Quote: Efficiency of all qPCR probes was tested by using a simplex 2-fold serial dilution of Quantigene commercial reference human RNA (Invitrogen) or DNA (Promega ...
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bioRxiv - Microbiology 2022Quote: A simplex end-point PCR was performed (on Thermocycler 2720, Applied Biosystems, Lincoln Centre Drive ...
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bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... and IL-1β concentrations were measured using a ProcartaPlex simplex assay (ThermoFisher, Waltham, MA).
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bioRxiv - Microbiology 2021Quote: ... Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T) with lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 1-2 x 106 antigen-experienced 5c.c7 TCR-transgenic T-cells were incubated with 2 µM Fura-2-AM (Life technologies) in T-cell growth medium for 15-20 minutes at room temperature and subsequently washed with warm (room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was performed with Low pH IHC Antigen Retrieval Solution (Invitrogen) in a pressure cooker set to 100°C for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval was performed using IHC Antigen Retrieval Solution (ThermoFisher, 00-4955-58) in a steamer for 40Dminutes ...
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bioRxiv - Cell Biology 2021Quote: The VEGF-A concentration in the culture medium was analyzed using a VEGF-A Human ProcartaPlex Simplex Kit (Cat. no. EPX01A-10277-901, Invitrogen), specific for the detection of VEGF-A165 ...
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bioRxiv - Cancer Biology 2023Quote: 20 μg protein per sample was used for the IFN-gamma mouse ProcartaplexTM Simplex High Sensitivity kit (Thermo Fisher Scientific) and the protocol was followed as per the product manual ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... relevant primers at 450 nM each and relevant TaqMan® probes (E380Q + REFex5 or Hotspot + REFex8 in simplex conditions or both assays combined in multiplex conditions) at 250 nM each (Applied Biosystems), DNA template (up to 8 μL ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated virus stocks in reduced-serum DMEM (2% FBS) supplemented with 50mM HEPES buffer (Gibco #15630080) were stored at -80°C ...
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bioRxiv - Immunology 2022Quote: ... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
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bioRxiv - Physiology 2022Quote: ... Targeted antigen retrieval was performed by boiling sections in 1x IHC antigen retrieval solution (Invitrogen) for 15-20 mins ...
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bioRxiv - Cell Biology 2023Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901), Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad ...
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bioRxiv - Genomics 2021Quote: ... cells or virus pellet were incubated with 2 mM of EZ-Link Psoralen-PEG3-Biotin (ThermoFisher Scientific) at 37°C for 10 min in PBS containing 0.01% digitonin ...
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bioRxiv - Microbiology 2021Quote: The spike of the SARS-CoV-2 virus (D614G variant) was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the codon-optimized sequence obtained by Ou et al ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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bioRxiv - Immunology 2023Quote: ... or control antigen PE (Thermo Fisher) were mixed with adjuvants per manufacturer’s protocols and added to cells immediately after plating ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μL of capture Ab (~4 μg/ml) or SARS-CoV-2 recombinant antigen (~0.2 mg/ml) in 1x PBS (Gibco) was loaded in each channel ...
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bioRxiv - Cancer Biology 2023Quote: ... The enzymatic antigen retrieval was performed by incubating the sections for 2 min with TrypLE™ Express Enzyme (ThermoFisher) at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated for 2 hours with the virus and then washed twice with Neurobasal-A medium (Gibco) containing 2% B-27 (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Microbiology 2023Quote: ... l ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for l h ...
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bioRxiv - Microbiology 2024Quote: ... and 2 U/µl of M-MulV-RT (Reverse transcriptase of Moloney Murine Leukemia Virus; Thermo Scientific, USA).
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bioRxiv - Microbiology 2023Quote: ... then 50 μl of sample or standard was added per well (in duplicate) with premixed beads in a 96-well plate according to the manufacturer instructions (ThermoFisher Procartaplex Human Simplex kit). The plate was incubated overnight on a shaker at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... brain slices underwent an additional antigen retrieval step in 1X IHC Antigen Retrieval Solution (Invitrogen, 00-4956-58) for 15 minutes at 95°C prior to blocking.
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
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bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.