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Citations for Thermo Fisher :
1 - 50 of 4675 citations for Hepatitis B Virus E Antigen HBeAg since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... C and S genes of the Hepatitis B virus (accession number: KY00323035) was ordered from GeneArt (ThermoFisher Scientific) and is available from Addgene (Addgene # 179155).
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
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bioRxiv - Bioengineering 2020Quote: ... William’s E medium was supplemented with Gibco Cell Maintenance Supplement Pack B (Cocktail B; Thermofisher Scientific), which contains bovine serum albumin (5.35 μg/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... media was changed to Hepatocyte Medium E (William’s E Medium [Gibco], Maintenance Cocktail B [Gibco], and 0.1µM Dexamethasone [Gibco]) for one day ...
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bioRxiv - Microbiology 2020Quote: ... N-term B virus mut) using a Neon® Transfection System (Invitrogen) under the recommended electroporation condition (1350 V ...
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bioRxiv - Developmental Biology 2023Quote: ... media was changed to Hepatocyte Medium E (William’s E Medium [Gibco], Maintenance Cocktail B [Gibco], and 0.1µM Dexamethasone [Gibco]) for one day ...
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bioRxiv - Molecular Biology 2023Quote: ... Abelson virus-transformed pre-B cells were maintained in DMEM (Thermo Fisher, 11960-077) supplemented with 10% FBS ...
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bioRxiv - Biophysics 2020Quote: ... soluble E-cadherin ectodomains were selected with 200 µg/mL Hygromycin B (Invitrogen). Western blots of the culture medium confirmed protein expression by individual colonies ...
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bioRxiv - Molecular Biology 2021Quote: Abelson virus-transformed pre-B cell lines were maintained in DMEM (Thermo Fisher #11960-077) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2024Quote: ... MM134-LTED-B/ MM134-LTED-E/SUM44PE-LTED-A/SUM44PE-LTED-B: IMEM with 10% charcoal stripped serum (CSS, Life Technologies), SUM44PE ...
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bioRxiv - Microbiology 2021Quote: ... Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T) with lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were infected with virus particles and selected with hygromycin B (200 µg/ml, Invitrogen). For single clone selection ...
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bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 pseudoviruses were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective spike protein into 293T cells (ATCC) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Immunology 2022Quote: ... B and E (+ SAgs) for 2 h in serum-free AIM V medium (Gibco, #12055-091). Unpulsed Raji B cells were used as negative control (-SAgs) ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were placed in a stereotaxic apparatus to receive bilateral infusions of the virus or 0.5% (w/v) cholera toxin subunit B (CTb) Alexa Fluor 555 Conjugate (Invitrogen) into the dorsal striatum (+0.1mm antero-posterior ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Immunology 2020Quote: ... Preformed conjugates for antigen-specific B cell sorting were made as described (46) using streptavidin conjugated to PE or AlexaFluor 647 (Life Technologies). B cell probes were made using clade B JR-CSF gp120 ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was performed with Low pH IHC Antigen Retrieval Solution (Invitrogen) in a pressure cooker set to 100°C for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Salivary glands from infected mosquitoes were hand dissected and collected in complete William’s B medium (William's E medium with Glutamax [Thermo Fisher, 32551-087] ...
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bioRxiv - Immunology 2023Quote: ... and APC-coupled anti-HLA-A/B/C/E Ab (RRID:AB_314879) in PBS/BSA with 1% saponin (ThermoFisher #C10424) prior to acquisition on a BD LSRFortessa.
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bioRxiv - Molecular Biology 2021Quote: ... was performed using double nucleic acid detection kit for respiratory syncytial virus (A/B) (Shenzhen shengkeyuan) with the ABI Q7 (Applied Biosystems). The reaction was conducted according to the manufacturer’s instructions with total volume of 20 μL.
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bioRxiv - Cancer Biology 2022Quote: ... except that some preparations were made using donor T cells that were activated for 48 hours before virus infection using LentiBoost (5 μl each of solutions A and B per ml of cells transfected; Fisher Scientific). 100 units/ml IL-2 was added at the start of the activation procedure and with every media change during the expansion phase.
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval was performed using IHC Antigen Retrieval Solution (ThermoFisher, 00-4955-58) in a steamer for 40Dminutes ...
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bioRxiv - Microbiology 2019Quote: ... were flanked by self-cleaving ribozymes (a hammerhead ribozyme upstream and a hepatitis delta ribozyme downstream) and the whole sequence was ordered (GeneArt, ThermoFisher Scientific, UK) and cloned into a pSF-CAG-KAN vector (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Salivary glands from infected mosquitoes were hand dissected and collected in complete William’s B medium (William's E medium with Glutamax [Thermo Fisher, 32551-087], supplemented with 1X insulin/transferrin/selenium [Thermo Fisher, 41400-045] ...
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bioRxiv - Immunology 2021Quote: ... T2 target cells and Epstein-Barr Virus-transformed B lymphoblastoid cell lines (EBV-LCLs) cells were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with 10% FCS and 1% penicillin/streptomycin (#P433 ...
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bioRxiv - Immunology 2022Quote: ... Fluorescent antigen probes were used to detect VRC01gHL B cells with eOD-GT8 60-mer conjugated to Alexa Fluor 647 (Thermo Fisher Scientific, Catalog # A20173). Intracellular staining was performed with a fixation kit (BD Bioscience ...
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bioRxiv - Immunology 2023Quote: ... from singly sorted antigen-specific memory B cells were RT-PCR amplified using SuperScript III and AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Waltham, MA) under conditions previously described46.
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bioRxiv - Synthetic Biology 2024Quote: MetBo2 (polyoma middle T oncogene–induced mouse mammary tumor on a syngeneic Friend Virus B NIH Jackson (FVB) background)[23] cells were maintained in 1X Dulbecco’s Modified Eagle Medium (DMEM) (Thermofisher Scientific; Cat. No. 11995065) with 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
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bioRxiv - Physiology 2022Quote: ... Targeted antigen retrieval was performed by boiling sections in 1x IHC antigen retrieval solution (Invitrogen) for 15-20 mins ...
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bioRxiv - Cell Biology 2023Quote: Overlay of EM and LM data (Fig. 3) and most of the segmentations (Fig. 4A,B,D,E) were done using Amira (Thermo Fisher Scientific). Additional segmentation was performed using Microscopy Image Browser (Belevich et al ...
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bioRxiv - Immunology 2023Quote: ... or control antigen PE (Thermo Fisher) were mixed with adjuvants per manufacturer’s protocols and added to cells immediately after plating ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Developmental Biology 2023Quote: ... media was changed to Hepatocyte Medium E (William’s E Medium [Gibco] ...
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bioRxiv - Cancer Biology 2024Quote: ... brain slices underwent an additional antigen retrieval step in 1X IHC Antigen Retrieval Solution (Invitrogen, 00-4956-58) for 15 minutes at 95°C prior to blocking.
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
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bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... Varicella zoster virus solution (Fisher Scientific) was dropped onto a No.1 coverslip and dried on top ...
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bioRxiv - Cell Biology 2023Quote: Cytotune OSKM Sendai virus (A16517, Invitrogen) and EmGFP reporter Sendai Virus (A16519 ...
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bioRxiv - Cell Biology 2019Quote: ... E-cadherin was visualized with rat anti-E-cadherin (Invitrogen #13-1900), both at 1:100 concentrations as per manufacturer protocol ...