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Citations for Thermo Fisher :
1 - 50 of 328 citations for Helicobacter Pylori Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... pylori (1:250) was from Thermo Scientific, USA ...
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bioRxiv - Immunology 2019Quote: ... pylori bacteria were grown in BHI broth (Oxoid, ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... pylori using Proteome Discovery 1.4 software (Thermo Fisher Scientific, San Jose, CA), with a differential modification of 274.18 m/z in the case of EBS and 150.15 m/z in the case of captan.
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bioRxiv - Microbiology 2020Quote: ... pylori were constructed and amplified using Escherichia coli one-shot top10 or DH5α strains (Thermofisher). H ...
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bioRxiv - Microbiology 2020Quote: ... pylori for 24 hours were extracted using ProteoJet™ Membrane Protein extraction kit (Fermentas, Thermo Scientific, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was performed with Low pH IHC Antigen Retrieval Solution (Invitrogen) in a pressure cooker set to 100°C for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval was performed using IHC Antigen Retrieval Solution (ThermoFisher, 00-4955-58) in a steamer for 40Dminutes ...
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bioRxiv - Microbiology 2021Quote: ... pylori was cultured in dialyzed Brucella broth with 10% (v/v) dialyzed fetal bovine serum (FBS; Gibco, US origin) in vented T25 flasks (Falcon ...
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bioRxiv - Microbiology 2022Quote: ... pylori challenged gastric epithelial cells were either treated by (i) the addition of 10 units Turbo DNase (Life Technologies) and incubation at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... pylori was cultured on Trypticase soy agar with 5% (v/v) sheep blood (Thermo Fisher Scientific, Waltham, MA, USA) for 2 days at 37°C in microaerobic conditions ...
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bioRxiv - Immunology 2022Quote: ... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
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bioRxiv - Physiology 2022Quote: ... Targeted antigen retrieval was performed by boiling sections in 1x IHC antigen retrieval solution (Invitrogen) for 15-20 mins ...
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bioRxiv - Microbiology 2022Quote: ... pylori NAP proteins (SARS-CoV-2S6p3 and SARS-CoV-2S6p312, respectively) were produced upon transient expression in Expi293F cells (ThermoFisher). Clarified supernatants were purified by affinity chromatography using an anti-HA affinity matrix (Cat# 11 815 016 001 ...
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bioRxiv - Microbiology 2023Quote: ... pylori cells were harvested from plates and resuspended in 2.5 ml of Sulfite-Free Brucella Broth containing cholesterol (Gibco-BRL) (SFBB-chol ...
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bioRxiv - Immunology 2023Quote: ... or control antigen PE (Thermo Fisher) were mixed with adjuvants per manufacturer’s protocols and added to cells immediately after plating ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
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bioRxiv - Microbiology 2022Quote: ... pylori genomic DNA (hp1421 locus, 290 bp fragment) and mtDNA (coxII, 444 bp fragment) by Fast SYBR Green chemistry (Life Technologies) on a Viia7 platform (Thermo) ...
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bioRxiv - Microbiology 2019Quote: ... pylori was washed twice with phosphate-buffered saline (PBS) and total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) as described in the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Cancer Biology 2024Quote: ... brain slices underwent an additional antigen retrieval step in 1X IHC Antigen Retrieval Solution (Invitrogen, 00-4956-58) for 15 minutes at 95°C prior to blocking.
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bioRxiv - Cancer Biology 2023Quote: ... after which heat-induced antigen retrieval was performed in 1x low pH antigen retrieval solution (pH6, Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... pylori harvested from blood agar plates after 20 h of growth into sterile cell culture PBS (1x concentration; Gibco, Thermo Fisher Scientific, USA). The bacterial suspension quantified by OD600 measurement was centrifuged and the pellets were stored at −20°C ...
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bioRxiv - Microbiology 2023Quote: ... pylori harvested from blood agar plates after 20 h of growth into sterile cell culture PBS (1x concentration; Gibco, Thermo Fisher Scientific, USA). The bacterial suspension quantified by OD600 measurement was centrifuged and the pellets were stored at −20°C ...
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bioRxiv - Neuroscience 2023Quote: ... A mild antigen retrieval step was introduced by immersing the slides into boiled antigen retrieval solution (00-4955-58, Invitrogen) for 10 min ...
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bioRxiv - Immunology 2020Quote: ... antigens were directly coated to Nunc-absorb (ThermoFisher) plates overnight at 4°C or captured using streptavidin ...
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bioRxiv - Immunology 2023Quote: ... Antigen printed pads were blocked with SuperBlock (ThermoFisher) for 1 hour before probed with serum samples (diluted at 1:100 in SuperBlock) ...
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bioRxiv - Physiology 2024Quote: ... heated in antigen retrieval solution (Thermo Fisher Scientific) at 100 °C for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... 4 × 105 mouse splenocytes/well were plated with either with 5 μg/ml YFV-17D NS3 ATLTYRML peptide antigen (57) or with 50 μg/ml of total Vero E6 cellular antigen in RPMI 1640 medium (Gibco, Belgium) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: NY-BR-1 antigen positive cell line CAMA-1 A2B2M and antigen negative SKMEL28 A2B2M were stained with CellTracker™ Deep Red Dye (Invitrogen, C34565) and seeded at 15000 and 10000 cells/well ...
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bioRxiv - Neuroscience 2024Quote: ... slices underwent an additional antigen retrieval step (10 minutes in a pressure cooker in IHC Antigen Retrieval Solution (Invitrogen: 00-4956-58), followed by cooling in ice for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for surface antigens in PBS (Gibco) or Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... using 20 μg/mouse of antigen in Alum (Invitrogen)7,8 ...
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bioRxiv - Immunology 2021Quote: ... Antigens were purified on C-tag column (Thermo Fisher) and quality-controlled by SDS-PAGE and for their level of endotoxin ...
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bioRxiv - Immunology 2020Quote: ... Antigens were retrieved using 2.5% trypsin (Thermo scientific, 15090046) in 0.1% HEPES buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and antigen retrieved in citrate buffer pH 6.0 (ThermoFisher) in water bath at 80°C for 5 mins ...
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bioRxiv - Developmental Biology 2023Quote: ... for antigen retrieval and 3% H2O2 (H325, Fisher Scientific) to inactivate endogenous peroxidase ...
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bioRxiv - Cancer Biology 2021Quote: ... Paraffin sections of all tumors and normal skin were deparaffinized and antigen retrieval was performed using heat induced epitope retrieval method (HIER) with 1X Antigen Retrieval solution (Invitrogen #00-4955-58) in a pressure cooker at 95 for 10 min ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and incubated with 10 μl 1:100 diluted plasma for 2h at 37°C to form immune-complexes ...
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bioRxiv - Immunology 2022Quote: ... biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and incubated with 10 μl 1:100 diluted plasma for 2h at 37°C to form immune complexes ...
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bioRxiv - Immunology 2022Quote: ... biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and to form immune-complexes incubated with 10 μl 1:10 diluted plasma samples for 2h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Citraconic anhydride for antigen retrieval was obtained from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Antigen/antibody complexes were visualized by Enhanced Chemiluminescence (Life Technologies) and signals were digitally acquired on a G:Box Chemi-XX6 imager with GeneSys software (Syngene) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antigen/antibody complexes were visualized by Enhanced Chemiluminescence (Life Technologies) and signals were digitally acquired on a G:Box Chemi-XX6 imager with GeneSys software (Syngene) ...
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bioRxiv - Cell Biology 2022Quote: ... followed by antigen retrieval in a 1x Citrate buffer (ThermoFisher) in a pressure cooker for one hour ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Bioengineering 2019Quote: ... antigens retrieved by treatmetn with 0.05 M EDTA (Ambion; AM9261) in a pressure cooker (Proteogenix ...
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bioRxiv - Physiology 2019Quote: ... Tissue nonspecific antigens were blocked using 0.5% NGS (#16210064, Invitrogen) dissolved in PBS for 1h at RT and using constant agitation ...
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bioRxiv - Cell Biology 2020Quote: ... or fluorescence-labeled secondary antibodies (for abundant antigens) (Thermo Fisher). For immunoblotting with HRP-conjugated secondary antibodies ...