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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Heat Shock Factor Protein 1 HSF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Heat-shock treated (Fisher Scientific) with 0.5% sodium azide to avoid any possible contamination to the blot ...
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bioRxiv - Microbiology 2024Quote: ... and Chlamydia heat-shock protein 60 (cHsp60) (ThermoFisher Scientific MA3-023, 1:2000 dilution), followed by secondary antibodies AlexaFluor 488 anti-mouse (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 2% heat shock fraction V BSA (Fisher Scientific, BP1600), 0.3% Triton-x-100 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... 2% heat shock fraction V BSA (Fisher Scientific, BP1600), 0.3% Triton-x-100 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Ligated plasmid products were introduced by heat shock transformation into competent Stbl3 (Invitrogen). The cloning products were confirmed using Sanger sequencing ...
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bioRxiv - Genomics 2022Quote: ... Pools were transformed by heat shock into Stbl3 competent cells (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... via heat shock as indicated by the provider and extracted with standard Miniprep (#K0503, ThermoFisher). The resulting plasmids were used in an LR reaction (Gateway LR clonase II enzyme mix ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was introduced by heat shock into DH5alpha and extracted with standard Miniprep (#K0503, ThermoFisher). The S71A and S71E mutations were introduced into the pDONR221-gCDC42attb plasmid with the QuickChange II XL Site directed mutagenesis kit (#200521 ...
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bioRxiv - Biochemistry 2023Quote: ... rabbit anti-HSF1 (Invitrogen, PA5-89420, 1:100), rabbit anti-SFPQ (Abcam ...
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bioRxiv - Genetics 2022Quote: ... An aliquot of the ligation mix was transformed into DH5α competent cells by heat shock (Thermo Scientific) and cells were grown overnight at 37°C incubated with LB broth and 1 mg/mL ampicillin ...
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bioRxiv - Immunology 2022Quote: ... This transfer plasmid (500 ng) was transformed using heat shock (42 °C, 1 min) into competent DH10 BAC cells (Thermo Fisher Scientific). Cells were cultured on agar plates containing selection antibiotics and Blu-gal ...
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bioRxiv - Genomics 2020Quote: ... Ligation reactions were transformed into DH5-α chemically-competent cells (42°C heat-shock, 45 seconds; Invitrogen 18265017) and ampicillin-resistant clones were screened by sanger sequencing ...
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bioRxiv - Immunology 2023Quote: ... the EEV was transformed into Stbl2 competent cells by heat shock following the manufacturer’s instructions (ThermoFisher, Cat. 10268019). After serial dilution in SOC media with ampicillin ...
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bioRxiv - Molecular Biology 2020Quote: ... and CRY2Clust (Park et al., 2017) coding sequences were inserted into heat-shock inducible Drosophila Gateway expression vectors (Life Technologies) to generate pHR-CRY2Clust-TRIM21 ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... 1 µL of crude reaction was used to transform NEB Stable competent cells by heat shock according to manufacturers’ instructions and grown at 32°C overnight on Luria-Bertani agar containing ampicillin (Gibco) at 100 mug/mL ...
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bioRxiv - Genomics 2020Quote: ... Ligation reactions were transformed into One Shot™ Stbl3™ chemically-competent E.coli cells (42°C heat-shock, 90 seconds; Invitrogen C737303) and positive clones were selected by Sanger sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then left untreated or subjected to heat-shock for 15 min prior to the addition of the methionine analogue L-azidohomoalane (AHA, 50 μM) (Thermo Fisher Scientific) and incubations continued for an additional 30 min.
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bioRxiv - Synthetic Biology 2022Quote: ... Vectors were transformed by heat-shock transformation into DH5α E.coli or One Shot™ ccdB Survival™ competent cells (Thermo Fisher Scientific). Cells were plated on lysogeny broth medium containing 100 μg mL−1 carbenicillin ...
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bioRxiv - Biochemistry 2022Quote: ... were transformed with the assembled vector using heat shock and plated on LB+agar plates containing 25 ug/mL zeocin (Gibco, cat. #R25001). Colonies were screened for correct DNA sequences using Sanger sequencing (Azenta) ...
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bioRxiv - Cell Biology 2021Quote: ... Hsf1(62) and Pgk1 (Thermo Fisher Scientific) and mouse monoclonal antibodies against GFP (Roche Diagnostics) ...
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bioRxiv - Cancer Biology 2023Quote: ... Epidermal Growth Factor protein (Gibco, USA). All the cell lines were grown at 37°C in a humidified chamber with 5% CO2 and authenticated by STR profiling.
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bioRxiv - Systems Biology 2019Quote: ... The stable HSF1:eGFP-transfected (HeLa-HSF1:eGFP) cell line was then established under selective pressure by 1000 μg/ml geneticin (Life Technologies) followed by selectionf of a single GFP-positive cell by flow cell sorting system (FACSAria III ...
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bioRxiv - Physiology 2020Quote: ... Additional larvae were subjected to a 4-hour heat shock at 35°C using a Fisher Scientific™ Isotemp heating plate (Thermo Fisher Scientific, Waltham, MA, USA). Larvae were sampled for gene expression immediately after the heat shock (“immediate” ...
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bioRxiv - Neuroscience 2023Quote: ... and human heat stable basic Fibroblast Growth Factor (bFGF) (Thermo Fisher Scientific PHG0369; 20 ng/ml) in vessels pre-coated with poly-L-ornithine hydrobromide (Sigma-Aldrich P3655 ...
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bioRxiv - Cancer Biology 2024Quote: ... and supplemented 1:2000 with Neuron Growth Factor (Ngf 2.5S Native Mouse Protein, Invitrogen) in the right ...
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bioRxiv - Neuroscience 2020Quote: ... 1% heat-inactivated FBS (Invitrogen, #10500064), 2% GLUTAMAX (Gibco ...
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bioRxiv - Neuroscience 2020Quote: Glial-Derived Neurotrophic Factor (GDNF) Recombinant Human Protein (ThermoFisher, #PHC7044)
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bioRxiv - Neuroscience 2020Quote: Brain-Derived Neurotrophic Factor (BDNF) Recombinant Human Protein (ThermoFisher, #PHC7074)
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bioRxiv - Systems Biology 2019Quote: ... and incubated for 90 min at RT with either a goat anti-mouse (for Hsc70/HSP72 expression) or a goat anti-rabbit (for HSF1 expression) secondary antibody conjugated to Alexa Fluor-594 (Life Technologies). A DNA-staining using Hoechst 33342 (1:10000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Serum shock was performed with 50% horse serum (Thermo Fisher Scientific) for 2 h when cells reached ~90% confluence ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... and 1:20 heat-inactivated mouse serum (Invitrogen), and then stained with a combination of the antibodies detailed in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... 1% heat inactivated fetal bovine serum (Life Technologies), in Ca2+/Mg2+ free 1X Dulbecco’s phosphate buffered saline (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... heat-inactivated (HIHS, 26050088, Gibco, 1% P/S) before SCs were liberated by treating with Collagenase II (100 units per 1ml ...
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bioRxiv - Cell Biology 2024Quote: ... heat-inactivated (HIHS, 26050088, Gibco, 1% P/S) before SCs were liberated by treating with Collagenase II (100 units per 1 ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Electric shock was carried out in 0.7X Optimem (Gibco, Thermofisher Life Sciences) with settings from (S ...
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bioRxiv - Molecular Biology 2023Quote: ... Electric shock was carried out in 0.7X Optimem (Gibco, Thermofisher Life Sciences) with settings from (S ...
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bioRxiv - Physiology 2023Quote: Maternal peripheral blood plasma immunomodulatory proteins and growth factors were analyzed with a Cytokine/Chemokine/Growth Factor 37-plex NHP ProcartaPlex kit (ThermoFisher Scientific). Plasma samples were run in duplicate on a Bioplex 200 instrument (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Human recombinant epidermal growth factor (hEGF) protein was purchased from Life Technologies and dissolved in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation (IP) of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human endogenous PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Hoechst 33342 (Invitrogen, C10247, dilution factor 1:2000) and AIF (Clone E-1 ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant insulin-like growth factor 1 (PHG0071; Gibco), recombinant leukemia inhibitory factor (SRP3316 ...
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bioRxiv - Cell Biology 2022Quote: ... epidermal growth factor (50 ng mL-1, ThermoFisher), Noggin (100 ng mL-1 ...
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bioRxiv - Biophysics 2023Quote: ... hypotonic shock was applied and cells were fixed with 4% paraformaldehyde (ThermoFisher Scientific) at different time points as indicated ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with transcription factor antibody (TOX; Thermo Fisher Scientific) in permeabilization buffer (eBioscience Transcription Factor Staining Set ...
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bioRxiv - Molecular Biology 2023Quote: ... visinin-like protein 1 primary antibody (Thermo Scientific Pierce Products) diluted 1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
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bioRxiv - Microbiology 2021Quote: ... 1% heat-inactivated fetal bovine serum (FBS) (Thermo Fisher Scientific), and 100 U/ml of penicillin and 100 μg/ml of streptomycin (Pen/Strep ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody is Rabbit polyclonal antibodies against green fluorescent protein (GFP) (1:500, Invitrogen). Secondary antibodies were conjugated with AlexaFluor-488 (1:500 ...
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bioRxiv - Immunology 2022Quote: Plasma was pooled and heat inactivated at 56°C for 1 hour then diluted in protein G binding buffer (Pierce ThermoFisher, catalog #54200) and passed over a column containing 1mL of protein A/G resin (Pierce ThermoFisher ...