Labshake search
Citations for Thermo Fisher :
1 - 50 of 3154 citations for Hantaan Virus Nucleoprotein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... Escherichia coli (E. coli) BL21-AI cells (Invitrogen) were transformed with a modified pET vector encoding N-terminal 10X His tagged VcINDY ...
-
bioRxiv - Bioengineering 2019Quote: ... fluorescent Escherichia coli (E. coli) BioParticles (ThermoFisher, cat. #E2863), or fluorescent carboxylate-modified 1 µm polystyrene beads (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: Glycerol stock of Escherichia coli (E. coli) (TOP10 strain, Invitrogen, Thermo Fisher Science ...
-
bioRxiv - Microbiology 2023Quote: ... The following primary antibodies were used: influenza A virus nucleoprotein (NP, PA5-32242, 1:3000, ThermoFisher Scientific), XPO1 (46249S ...
-
bioRxiv - Molecular Biology 2024Quote: ... coli poly(A) polymerase (E-PAP, Invitrogen) at 37°C for 30 min in 50 µL reactions ...
-
bioRxiv - Microbiology 2024Quote: ... To transform Stbl3 E coli cells (Invitrogen) with the BAC construct ...
-
bioRxiv - Microbiology 2019Quote: ... USA. Escherichia coli DH5α (hereafter called E. coli) was purchased from Invitrogen Inc. ...
-
bioRxiv - Microbiology 2023Quote: ... Escherichia coli strain DH5α (E. coli DH5α, Thermo Fisher Scientific, Cat#18258012), E ...
-
bioRxiv - Microbiology 2023Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
-
bioRxiv - Biophysics 2020Quote: CLGltPh and double cysteine mutants were transformed into Escherichia coli (E. coli) Top10 cells (Invitrogen). Bacterial cells were inoculated in Luria Broth medium containing 100 μg/mL ampicillin at 37 °C until OD600 ~0.6-0.8 ...
-
bioRxiv - Neuroscience 2020Quote: ... and 0.09 μL Ambion RNase H (E. coli) (Invitrogen, AM2293). Second strand synthesis was performed by incubating samples at 16°C for 150 min ...
-
bioRxiv - Genomics 2019Quote: ... 1 µl of 2 U/µl E Coli RNaseH (Invitrogen) and then incubating at 16°C for 2 hours ...
-
bioRxiv - Developmental Biology 2021Quote: Escherichia coli (E. coli) strain DH5α (Toyobo, Osaka, Japan) and BL21(de3) pLysS (C606003, ThermoFisher Scientific) were used for DNA cloning and protein expression ...
-
bioRxiv - Genomics 2022Quote: ... vector and amplified in DH5α strain of Escherichia coli (E coli) (Life Technologies, Grand Island NY). After amplification ...
-
bioRxiv - Developmental Biology 2021Quote: Escherichia coli (E. coli) strain DH5α (Toyobo, Osaka, Japan) and BL21(de3) pLysS (C606003, ThermoFisher Scientific) were used for DNA cloning and protein expression ...
-
bioRxiv - Microbiology 2024Quote: Glycerol stock of Escherichia coli (E. coli) (TOP10 strain, Invitrogen, Thermo Fisher Science, Waltham, MA, USA) transformed with plasmid pCR2.1 (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
-
bioRxiv - Biochemistry 2020Quote: ... were expressed in the bacterial Escherichia coli (E. coli) BL21(DE3) pLysS strain (Invitrogen, Thermo Fisher Scientific) as described in 38 ...
-
bioRxiv - Biochemistry 2020Quote: ... were expressed in the bacterial Escherichia coli (E. coli) BL21(DE3) pLysS strain (Invitrogen, Thermo Fisher Scientific) as described in 38 ...
-
bioRxiv - Immunology 2020Quote: ... 20⧅l of opsonised Escherichia coli (E.coli) was added to the tube (pHrodo Red E. coli BioParticles; ThermoFisher). The bacteria was initially prepared by mixing the E.coli strain with new-born calf sera followed by a 30-minute incubation at 37°C ...
-
bioRxiv - Biochemistry 2020Quote: ... All the SAM with natural isotope abundance and 2H or 13C enrichment were enzymatically synthesized from L-methionine and ATP with appropriate isotope labels. Escherichia coli (E. coli) DH5α and BL21(DE3) competent cells were from Invitrogen. Data plotting and nonlinear least-squares fitting was carried out using GraphPad Prism 7 software ...
-
bioRxiv - Biochemistry 2021Quote: ... and TnsB (WP_084763316.1) proteins were codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The ShCas12k gene was inserted into the 1B (Addgene 29653 ...
-
bioRxiv - Immunology 2019Quote: ... or pH sensitive fluorescent bioparticles (pHrodo E. coli Bioparticles Conjugate for Phagocytosis; Invitrogen). Each fly was injected with 27.6-32.2 nl of bioparticles (1mg/100µl ...
-
bioRxiv - Biochemistry 2019Quote: ... Filtrate was added onto DNA Polymerase 1 (PolI) (E. coli source, Fisher Scientific), 0.25U/uL reaction ...
-
bioRxiv - Biophysics 2024Quote: ... Ligated vectors were transformed into competent bacteria (One Shot TOP10 E. coli; Invitrogen) and grown overnight on ampicillin plates ...
-
bioRxiv - Cell Biology 2022Quote: ... DNA polymerases and NEBuilder HiFi DNA assembly master mix were obtained from New England Biolabs. Cloning was performed in TOP10 chemically competent Escherichia coli (E. coli) (ThermoFisher Scientific). Buffers for assessing fluorescence lifetime were composed of either 0.01M PBS (pH 6.5 – 7.4 ...
-
bioRxiv - Biochemistry 2022Quote: ... and Escherichia coli S15 (AP009048.1) proteins were codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The ShCas12k gene was inserted into the 1B (Addgene 29653 ...
-
bioRxiv - Cell Biology 2022Quote: ... All synthetic oligonucleotides were obtained from Integrated DNA technologies (IDT). Cloning was performed in TOP10 chemically competent Escherichia coli (E. coli) (ThermoFisher Scientific). mApple and TfR DNA was obtained from mApple-Lysosomes-20 (RRID:Addgene_54921 ...
-
bioRxiv - Biophysics 2022Quote: ... novicida U112 (Fn)Cas12a (WP_003040289) was codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The FnCas12a gene was inserted into the 1B plasmid (Addgene #29653 ...
-
bioRxiv - Neuroscience 2023Quote: ... 15 μg/mL and 50 000 cells/mL respectively. Bioparticles of pHrodoTM Green-labelled Escherichia coli (E. coli) were purchased from Thermo Fisher Scientific and used at a concentration of 25 μg/mL ...
-
bioRxiv - Neuroscience 2020Quote: ... plasmids were transformed into chemocompetent bacteria (E. coli Top10™, Thermo Fisher Scientific, TFS), which were generated like previously described70 ...
-
bioRxiv - Bioengineering 2023Quote: MMP9-pro-cat-Hiswt (E coli expression) was expressed in BL21(DE3) (Thermo Fisher). Cells were grown in 2xYT at 37°C and expression was induced with 0.5 mM isopropyl ?-D-1-thiogalactopyranoside (IPTG ...
-
bioRxiv - Biophysics 2020Quote: The plasmid pET3a containing human AβM42 and AβMC40 cDNA was transformed into Escherichia coli (E. coli) One Shot® BL21 (DE3) pLysS (Thermo Fisher Scientific, USA). The plasmids were a kind gift from Prof ...
-
bioRxiv - Cell Biology 2020Quote: ... vector restriction digests or DNA oligonucleotides with compatible overhangs. Cloning was performed in TOP10 chemically competent Escherichia coli (E. coli) (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2023Quote: ... with the resulting plasmid confirmed as correct by Sanger sequencing (Genewiz). The Escherichia coli (E. coli) DH5-α strain (Thermo Fisher Scientific) was used for plasmid amplification ...
-
bioRxiv - Microbiology 2021Quote: ... antibodies targeting the SARS-CoV-2 nucleoprotein (N) (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... The pET24b Cd-IF1 plasmid was subsequently transformed into One Shot™ BL21(DE3) Chemically Competent Escherichia coli (E. coli) (Thermo Fisher Scientific) for expression of the recombinant proteins.
-
bioRxiv - Microbiology 2022Quote: ... Transformations were performed in Top10 competent bacteria (One Shot TOP10 chemically competent E. coli, Invitrogen) and selected using 100 µg/mL ampicillin or/and 34 µg/mL chloramphenicol (Euromedex) ...
-
bioRxiv - Molecular Biology 2019Quote: ... polyA-tailed using Escherichia coli poly(A) Polymerase (E-PAP) and ATP (Thermo Fisher Scientific), and recovered using a MEGAclear kit (Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli cell lysate was prepared from the RNAse E deficient BL21 STAR (DE3) (ThermoFisher Scientific) cells that were transformed with the pRARE vector from BL21 Rosettta (Merck) ...
-
bioRxiv - Microbiology 2020Quote: ... Transformations were performed in Top10 competent bacteria (One Shot™ TOP10 Chemically Competent E. coli, Invitrogen) and selected using 100 μg/mL ampicillin or 50 μg/mL kanamycin sulphate (Euromedex) ...
-
bioRxiv - Cell Biology 2021Quote: Ocn-GFPTopaz mice were injected intravenously with 50mg/kg of pHrodo labeled E-Coli particles (Invitrogen) and one-hour post injection mice were sacrificed ...
-
bioRxiv - Cell Biology 2022Quote: ... LPS (E. coli 0111:B4, Ultrapure, tlrl-3pelps, used to treat cells) were obtained from Invitrogen. ATP-Na+ (A2383) ...
-
bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 pseudoviruses were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective spike protein into 293T cells (ATCC) ...
-
bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
-
bioRxiv - Cancer Biology 2022Quote: ... After transformation in bacteria (One Shot™ TOP10 Chemically Competent E. coli; Thermo Fisher Scientific, Bleiswijk, Netherlands), successful cloning was validated by sequencing ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
-
bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
-
bioRxiv - Cell Biology 2023Quote: ... for 5 min and hybridized with smFISH probes diluted 1:3000 in hybridization buffer (10% dextran sulfate, Sigma D8906, 20% formamide, 1 mg/ml E. coli tRNA, Sigma R1753, 2× SSC, 0.02% BSA, Ambion AM2616 ...