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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... or glutathione-S-transferase (GST) (Enzo Life Technologies) as a control protein ...
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bioRxiv - Immunology 2019Quote: ... and anti-glutathione-S-transferase (GST) antibody (ThermoFisher 71-7500). Antibody-bound complexes were collected ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... in a donor vector (pDONR-221) and transferred by recombinant cloning into the glutathione S-transferase (GST) fusion vector pDEST15 (Invitrogen). The plasmid was used to transform Escherichia coli ...
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bioRxiv - Cell Biology 2022Quote: The TNY1 cDNA coding sequences were cloned into the Gateway pDEST15-GST (glutathione S-transferase) plasmid using the procedures recommended by the manufacturer (Invitrogen) with oligos listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Glutathione-S-Transferase-protein complex was cut using TEV protease (Invitrogen), and further purified with size-exclusion chromatography with HiLoad 26/60 Superdex 75 column (GE Healthcare) ...
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bioRxiv - Plant Biology 2021Quote: ... to create a fusion to the Maltose-binding protein (MBP) or into pDEST15 for fusions to Glutathion-S-transferase (GST) (ThermoFisher). To create GUS reporter constructs ...
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bioRxiv - Genomics 2020Quote: ... were amplified via the polymerase chain reaction (PCR) using primers to facilitate subsequent ligation of the PCR products into the glutathione-S-transferase encoding vectors (Life Technologies). The M.MspI PCR product was initially inserted into the BamHI and EcoRI sites in pGEX 2T ...
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bioRxiv - Plant Biology 2021Quote: ... and GST-△F-box-AFF1 proteins were immobilized on GST beads (Glutathione Agarose; ThermoFisher). Immobilized agarose beads containing 2 μg GST ...
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bioRxiv - Cell Biology 2020Quote: ... GST-NtRac5 were immobilized on glutathione-agarose (Thermo Fisher Scientific) and incubated with purified recombinant MBP-AtPIP5K2 protein for 60 min at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... the GST-RNF38RING was incubated with glutathione agarose (Thermo Fisher). The UBC13~Ub conjugate was mixed with GST-RNF38RING (60 μM ...
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bioRxiv - Plant Biology 2020Quote: ... The GST and GST-XIK fusion proteins were extracted with Pierce Immobilized Glutathione resin (Thermo Fisher Scientific) with the protein interaction buffer (20 mM Hepes-KOH pH7.2 ...
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bioRxiv - Cell Biology 2019Quote: ... GST or GST-EB1 (5 μg) were incubated with 20 μl glutathione agarose beads (16100; Thermo Scientific) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-tagged NgAgo variants were purified by glutathione agarose (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... and CMG2-GST was affinity purified with Glutathione Superflow Agarose (Thermo Fisher). Glutathione was removed and CMG2 exchanged into HBS-T with 50% glycerol using Sephadex G50 (GE Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... GST proteins were captured on Pierce glutathione magnetic beads (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... bacterially expressed GST-p21 bound to glutathione Sepharose beads (Thermo Scientific, 16100) were incubated with His-RanBPM ...
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bioRxiv - Microbiology 2023Quote: ... GST-RILP or GST proteins were purified by using a pre-equilibrated slurry of glutathione beads (Thermo Scientific, #16100) in TBS containing 1mM MgCl2 and washed three times in the same buffer containing 0.05% Triton X-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific). On-bead recombinant proteins were washed with the respective lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: ... RAP-GST was eluted in Buffer A with 25 mM glutathione (Fisher Scientific). Fractions confirmed by Coomassie were dialyzed in a dialysis buffer (20 mM HEPES ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins (5 µg) along with GST alone were incubated with 10 µl of magnetic glutathione beads (Thermo Fisher Scientific) for one hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The GST- fusion proteins were purified under native conditions using glutathione agarose (ThermoFisher Scientific) and the LRRCC1 fragments were recovered by Tev protease cleavage and dialyzed before rabbit immunization (Covalab) ...
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bioRxiv - Genomics 2022Quote: ... The 15N-GST-H4 peptide fusion was purified on glutathione agarose resin (Thermofisher Scientific), cleaved with PreScission Protease (16 hr incubation at 4°C) ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant corresponding to GST-tev-JMJD6 (1-403) was loaded to 7mL of glutathione agarose resin (Thermo Scientific), washed with nickel-binding buffer ...
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bioRxiv - Plant Biology 2021Quote: ... GST-MdCE was purfied by Pierce™ Glutathione Spin Columns (16105, Thermo Scientific™, USA) and MBP-MdRNF4 was purified by MBP TRAP HP (GE Healthcare) ...
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bioRxiv - Cancer Biology 2019Quote: ... bait GST-tagged EIF4E or 6xHis-tagged CELF1 proteins (1 μM) were initially bound to glutathione-agarose beads (ThermoFisher Scientific) or Ni2+ beads (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... and the GST-fusion protein was purified employing standard procedures using glutathione Sepharose beads (Thermo Scientific).
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bioRxiv - Biochemistry 2021Quote: ... the recombinant GST-SHP2 in supernatant was affinity-purified by Pierce glutathione agarose (Thermo Fisher Scientific) and eluted with lysis buffer containing 20 mM GSH ...
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bioRxiv - Microbiology 2020Quote: ... GST fusion proteins were purified on glutathione superflow agarose according to the manufacturer’s protocol (Thermo Scientific). The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-mCRD conjugated on Glutathione Sepharose 4B beads and a synthetic ssDNA library (ThermoFisher Scientific, NC1108024) of random 30-mer sequences flanked by 23 nt at each end (5’-TAG GGA AGA GAA GGA CAT ATG AT-3’ and 5’-TTG ACT AGT ACA TGA CCA CTT GA-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... GST-Arl15 immobilized on glutathione Sepharose beads was incubated with 50 mM dimethyl pimelimidate (Thermo Fisher Scientific) in 200 mM sodium borate pH 9.0 to crosslink GST-Arl15 covalently onto glutathione Sepharose beads ...
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bioRxiv - Immunology 2023Quote: ... The supernatant fraction containing the GST-tagged constructs were loaded onto Protino Glutathione Agarose 4B (Fisher Scientific) beads and impurities were removed by washing with TBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Free and total GSH were measured using a glutathione fluorescent detection kit (cat.no. EIAGSHF) according to the manufacturer’s protocol (Invitrogen, Carlsbad, CA).
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bioRxiv - Cancer Biology 2021Quote: ... Total glutathione (GSH) content was measured using the Glutathione Colorimetric Detection Kit (Invitrogen) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... 5 µg of GST-fused bait were coupled to Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602), previously washed twice with equilibration buffer (Tris-HCl 125 mM ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μg of GST tagged protein was conjugated to Pierce™ Glutathione Magnetic Agarose Beads (Thermo Fisher, 78602), for 1hr at RT ...
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bioRxiv - Physiology 2022Quote: ... GST-HGK was removed from the reaction using 10 μL of Glutathione Sepharose beads (Thermo Fisher Scientific #45000285) and batched incubation at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... GST-AAK1 (1-501aa) (Thermofisher) with or without GST-AP2M1-WT or T156A protein were subjected to a kinase reaction in kinase buffer (20 mM HEPES pH=7.4 ...
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bioRxiv - Microbiology 2019Quote: ELISA was performed using anti-GST coated strips (Pierce™ Anti-GST Coated Clear Strip Plates, Thermo Scientific). A fixed amount of 10 pmol GST-tagged PA1217 was diluted in 200 µl PBS (137 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Phages presenting NLRP1PYD-specific VHHs were enriched using GST-NLRP1PYD immobilized to magnetic Pierce glutathione beads (Thermo Fisher Scientific). The retained phages were used to infect E ...
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bioRxiv - Microbiology 2023Quote: ... Anti-GST pull-downs were completed with these protein suspensions subsequently incubated with Pierce™ Glutathione Agarose (ThermoFisher 16100) for an hour at 4°C in binding buffer (PBS pH 7.0 with 1 mM Tris (2-carboxyethyl ...
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bioRxiv - Cell Biology 2023Quote: ... GST-TAF3 PHD proteins were purified using the Pierce GST Spin Purification Kit (ThermoFisher) and dialyzed against 20 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... plates (Nunc A/S, Roskilde, Denmark) were coated with proteins from bacterial lysates at 3 µg/ml in 0.5 M carbonate buffer pH 9.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Liver glutathione sulfhydryl (GSH) measurements were performed using Glutathione Colorimetric Detection Kit (EIAGSHC, Thermo Fisher). All colorimetric assays were performed according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Molecular Biology 2020Quote: GST or GST-PHF1-tudor proteins (10 μg each) were incubated with 4 μl (25% slurry) of Glutathione Magnetic Beads (88821; Thermo Scientific) in 250 μl of binding buffer (125 mM Tris-HCl pH8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...