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Citations for Thermo Fisher :
1 - 50 of 4786 citations for G3BP1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... anti-G3BP1 (ThermoFisher, PA5-29455), anti-dsRNA J2 (SCICONS ...
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bioRxiv - Molecular Biology 2024Quote: ... G3BP1 (Invitrogen, Cat# PA5-29455) or EDC4 antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were successively incubated with primary antibodies diluted in blocking buffer (mouse anti-G3BP1, 1:4000, BD Biosciences, rabbit anti-GFP, 1:1000, ThermoFisher, mouse anti-PKR ...
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bioRxiv - Neuroscience 2022Quote: HeLa cells stably expressing mCherry-G3BP1 were established by transfection of the mCherry-G3BP1 construct using Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s instructions and subsequent multiple rounds of G418 (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: Sorted HSC were also cultured with blocking CD150 peptide (FCKQLKLYEQVSPPE, Auspep, 100 μg/ml) or control peptide (DLSKGSYPDHLEDGY, Auspep,100 μg/ml) (Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: G3BP1 antibody (1:1000 dilution, Invitrogen, Cat# PA5-29455).
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bioRxiv - Cell Biology 2024Quote: ... GFP-G3BP1 signal was visualized in FluoroBrite™ DMEM (Gibco) in the same microscope ...
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bioRxiv - Cancer Biology 2019Quote: ... HS00428478-G3BP1 and HS00163427-USP1 were purchased from ThermoFisher (Waltham, MA). The mRNA expression was analyzed using multiplex PCR for the targeted gene of interest and GAPDH as reference using two independent detection dyes FAM probes and VIC probes respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-G3BP1 rabbit polyclonal antibody (ThermoFisher; 13057-2-AP; 1:600 dilution); anti-SRAS-CoV-2 Nucleocapsid rabbit polyclonal antibody (GeneTex ...
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bioRxiv - Cell Biology 2024Quote: ... U2OS G3BP1-GFP cell line was generated using Flp-In system (ThermoFisher), details below ...
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bioRxiv - Developmental Biology 2021Quote: ... After blocking with blocking buffers (Thermo Scientific) for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-G3BP1 antibody-bound SG cores were collected through the incubation with Protein G Dynabeads (Invitrogen) with consecutive SG RNA elution from the Dynabeads by Proteinase K (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... U2OS stably expressing G3BP1-GFP were seeded into eight-well Lab-Tek chambered cover glass (Nunc) for sodium arsenite experiments or 35-mm glass-bottom dishes (MatTek ...
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bioRxiv - Cell Biology 2023Quote: G3BP1-GFP cells were seeded in 4-well Lab-Tek II Chambered Coverglass (Thermo Scientific; 1055360). For pre-incubation experiments ...
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bioRxiv - Cell Biology 2022Quote: ... and then blocking in Superblock Blocking Buffer (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was performed using BlockAid Blocking Solution (Thermo Fisher Scientific). Primary antibody incubation against clathrin heavy chain (ab21679 from Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... Blocking Buffer (Invitrogen Cat# 00-4952-54 ...
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bioRxiv - Microbiology 2021Quote: ... and blocking in SuperBlock Blocking Buffer (Thermo Fisher Scientific, Waltham, MA). Cleaved caspase-3 was immunostained by incubating HAE with a primary human cleaved caspase-3 (Asp175 ...
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bioRxiv - Cancer Biology 2019Quote: ... After blocking with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific), the plates were incubated with culture supernatant with subsequent 1:2000 diluted peroxidase-conjugated anti-mouse IgG (Agilent Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... before blocking in SuperBlock™ blocking buffer (Thermo Fisher Scientific, 37515). Primary antibodies (MAP2 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... followed with endo-biotin blocking using a blocking kit (Invitrogen, #E21390) according to the manufacture instructions [118] ...
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bioRxiv - Microbiology 2023Quote: ... blocking was performed overnight using SEA BLOCK Blocking buffer (ThermoFisher, 37527) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: GFP-ki-G3BP1 WT and shPARP10 cells were seeded in 2-well chambered cover glass (Nunc Lab-Tek) at around 70% confluency ...
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bioRxiv - Microbiology 2023Quote: ... The peptide concentrations of eluted peptides were quantified using the Pierce Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were quantified with Quantitative Colorimetric Peptide Assay (Thermo Scientific) and 5 µg of total peptides were labeled using TMTpro16-plex reagents (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were desalted with peptide desalting spin columns (Thermo Scientific) and dried via vacuum centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Biochemistry 2023Quote: Peptide samples were assayed for peptide concentration with Pierce™ Quantitative Colorimetric Peptide Assay (Thermofisher Scientific, Brisbane, Australia), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... U2OS or HeLa cells stably expressing G3BP1-GFP were seeded into eight-well Lab-Tek chambered cover glass (Nunc). At least 20 min prior to the experiment ...
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bioRxiv - Biochemistry 2024Quote: ... and G3BP1 were labeled on their N-termini with AF488 (Alexa Fluor 488 carboxylic acid succinimidyl ester, Thermo Fisher). Storage buffer was exchanged to Labeling buffer (50 mM MES pH 6.5 ...
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bioRxiv - Microbiology 2022Quote: ... Labelled peptides were quantified (Pierce Colorimetric Peptide Assay Kit, Thermo Scientific) and mixed 1:1:1 based on peptide amount.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were quantified using a colorimetric peptide assay (Thermo Fisher Scientifc). Peptides (1 µg ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using colourimetric peptide assay (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using a colorimetric peptide assay (ThermoFisher Scientific). All samples were reconstituted in loading buffer (5% CH3CN ...
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bioRxiv - Biophysics 2021Quote: ... blocking of endogenous biotin was performed using a commercial blocking kit (Invitrogen, E21390). Cells were incubated in component A for 45 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... After blocking with 10% goat serum or Superblock blocking buffer (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Peptide digest assay standard (PierceTM Quantitative Colorimetric Peptide Assay, Thermo Scientific, USA) was as standard for quantitative analysis of peptides ...
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bioRxiv - Cell Biology 2022Quote: ... peptide concentration was measured using a colorimetric peptide quantification assay (Thermo Scientific). Finally ...
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bioRxiv - Systems Biology 2019Quote: ... Peptides were quantified using Pierce Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions on a Varioskan LUX Multimode Microplate Reader and SkanIt 5.0 software (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Peptide concentration was determined by a quantitative colorimetric peptide assay (Thermo Fisher), and 1 μg aliquots of clean peptides from each sample were injected sequentially over a nano-flow C18 column (EasySpray ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide concentrations were determined using Pierce Quantitative Fluorometric Peptide Assay (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... peptides were desalted on PierceTM Peptide Desalting Spin Columns (Thermo Fisher Scientific) and concentrated in a SpeedVac concentrator.
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bioRxiv - Microbiology 2021Quote: ... The peptide digests were then cleaned up using peptide desalting columns (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... for 48 h then transfected for 24 h with 1 ug His-G3BP1 3’UTR constructs using Lipofectamine LTX and Plus reagent (Invitrogen). To determine mRNA stability ...
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bioRxiv - Molecular Biology 2021Quote: ... or stress granules (G3BP1 antibody, Supplementary File 6) the cells were mounted along with 0.1 µm TetraSpeck™ microspheres (ThermoFisher) adhered to the slide according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... To measure colocalization of TCRA and TCRB mRNAs with each other or with P bodies (using DCP1 antibody) or stress granules (G3BP1 antibody) the cells were mounted along with 0.1 µm TetraSpeck™ microspheres (ThermoFisher) adhered to the slide according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... After washing with PBS-T and blocking with StartingBlock (TBS) blocking buffer (ThermoFisher Scientific), serially diluted serum from groups of immunized mice or baboons were added to coated wells and incubated for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... followed by blocking with Starting Block T20 blocking buffer (Thermo Fisher Scientific catalog #37539) for 13 minutes ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... following peptide quantification (ThermoFisher). All samples were subjected to nanoLC-MS/MS analysis using an UltiMate 3000 RSLCnano system (Thermo Fisher ...