Labshake search
Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... fatty acid free FBS (Gibco #A3382101), L-Glutamine (Gibco #25030-123) ...
-
bioRxiv - Cell Biology 2020Quote: ... Fatty Acid-Free Powder (Thermo Fisher Scientific – BP9704100), Fetal bovine serum (Wisent – 095-150) ...
-
bioRxiv - Cancer Biology 2023Quote: ... anti-fatty acid synthase (1:50, Invitrogen MA5-14887), anti-LC3B (1:200 ...
-
bioRxiv - Microbiology 2022Quote: Virus particle alterations were characterized by a thermofluorometric dual dye-binding assay using the nucleic acid dye SYTO9 and the protein-binding dye SYPRO orange (both from Invitrogen). Reaction mixtures of 50 μl containing 1.0 μg of purified virus ...
-
bioRxiv - Biochemistry 2023Quote: ... Pre-incubated mixture of scFv and respective whole venom was mixed with high-performance His-tag binding cobalt resin beads provided with Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, USA) and incubated at 20°C for 12 hours to allow attachment of the complex on resin via His-tag ...
-
bioRxiv - Molecular Biology 2022Quote: ... The following fatty acid azides were used: C15-az (15-azidopentadecanoic acid) from Life Technologies and C17-az (17-azidoheptadecanoic acid ...
-
bioRxiv - Immunology 2023Quote: ... Fatty acid uptake was analyzed by flow cytometry using Bodipy (Invitrogen, Bodipy FL C16 ...
-
bioRxiv - Biochemistry 2020Quote: ... Binding of the full-length S protein was detected by mouse anti-His IgG (Invitrogen MA121315, 2 μg/ml) and Alexa Fluor-488-labelled goat anti-mouse IgG (Invitrogen A11001 ...
-
bioRxiv - Immunology 2023Quote: ... protein-binding plates (Nunc Maxisorp) were coated with rMOG (housemade ...
-
bioRxiv - Developmental Biology 2020Quote: ... was captured using a GFP binding protein (GBP) covalently conjugated to carboxylic acid decorated Dynabeads (Dynabeads M-270 carboxylic acid, Thermo Fisher Scientific). Untagged SMO (Fig ...
-
bioRxiv - Bioengineering 2021Quote: ... or a long-chain fatty acid (Hexadecanoic Acid, C16; BODIPY™ FL C16, D3821, Thermo Fisher Scientific Inc.) were added at a concentration of 4 µM to the culture medium as defined by culture mode (table 3) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: The unbound fraction of diclofenac in the presence of FABP1 was directly measured using Pierce Nickel-nitrilotriacetic acid (Ni-NTA) Magnetic Agarose Beads (Thermo Fisher Scientific, Waltham, MA) for all diclofenac concentrations used in kinetic experiments ...
-
bioRxiv - Bioengineering 2021Quote: ... a medium-chain fatty acid (Dodecanoic Acid, C12; BODIPY™ 500/510 C1, C12; D3823, Thermo Fisher Scientific Inc.) or a long-chain fatty acid (Hexadecanoic Acid ...
-
bioRxiv - Cell Biology 2019Quote: ... Egg sphingomyelin: fatty acid free BSA complexes were made by dissolving in Optimem (Gibco) at a molar ratio of 1000:1 (sphingomyelin:BSA ...
-
bioRxiv - Cell Biology 2022Quote: ... and fatty acid free bovine albumin (BSA) were obtained from Fisher Scientific (Rochester, NY). All commercial reagents were used without further purification.
-
bioRxiv - Genetics 2022Quote: ... stained with the LD marker Bodipy 558/568 C12 fatty acid (Molecular Probes, D3835) and LD diameters were measured in 100 to 150 cells from three independent experiments using Imaris software (Bitplane ...
-
bioRxiv - Cell Biology 2020Quote: ... before blocking for 1 hour in denatured fatty acid free 1% BSA (Life Technologies). Finally coverslips were washed once with PBS before the addition of platelets ...
-
bioRxiv - Biophysics 2023Quote: ... and fatty acid-free bovine albumin (BSA) were obtained from Fisher Scientific (Rochester, NY). All commercial reagents were used without further purification.
-
bioRxiv - Molecular Biology 2022Quote: ... PfP2 sample was purified after binding with Hi-Bind Ni-NTA beads (Invitrogen) for 4h followed by elution of the sample with 20mM Tris-HCl (pH 7.4) ...
-
bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
-
bioRxiv - Cell Biology 2022Quote: ... free fatty acids were extracted from cells using 90% methanol (LC-MS grade, Thermo Scientific). The extracts were clarified by centrifugation at 15,000 x g for 10 min ...
-
bioRxiv - Molecular Biology 2023Quote: Free fatty acids were extracted from serum using 90% methanol (LC/MS grade, Thermo Scientific). The extracts were clarified by centrifugation at 15,000 g for 10 min ...
-
bioRxiv - Cell Biology 2021Quote: ... placed in a low binding tube (Low Protein Binding Microcentrifuge tubes, Thermo Scientific). Subsequently ...
-
bioRxiv - Cell Biology 2023Quote: ... High protein-binding MaxiSorp plates (ThermoFisher Scientific) were coated with 200 ng/well soluble LCMV sGP resuspended in 1x TBS and incubated overnight at room temperature ...
-
bioRxiv - Microbiology 2019Quote: ... 6×His-Lsr2 was purified by binding to 1 mL Ni-NTA agarose (Invitrogen), after which the resin was collected and the bound protein was washed with binding buffer supplemented with increasing concentrations of imidazole ...
-
bioRxiv - Genetics 2022Quote: ... FRAP was performed on 3T3-L1 preadipocytes transiently transfected with GFP-tagged CIDEC were incubated with 200 μM of oleic acid and stained with Bodipy 558/568 C12 fatty acid (Molecular Probes, D3835) for 15 hours ...
-
bioRxiv - Bioengineering 2020Quote: Fatty acid-free bovine serum albumin (BSA) was purchased for use in this study (Fisher Scientific). All chemical reagents were purchased from Sigma-Aldrich unless otherwise indicated.
-
bioRxiv - Immunology 2021Quote: ... The proteins were then purified by immobilized metal affinity chromatography by using HisPur Ni-NTA (Purify His-tagged recombinant fusion proteins using high-capacity nickel-nitrolotriacetic acid methal-chelate beads) spin columns (ThermoFisher). Recombinant proteins were refolded on the column by gradually removing urea until reaching to a concentration 0 M and then dialyzed against PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... Mouse α-TATA Binding Protein (ThermoFisher 49-1036), Goat α-mouse:HRP (Novus Biologicals NBP2-31347H) ...
-
bioRxiv - Microbiology 2021Quote: ... The cross connector separates the injection ports for the fatty acid dye (D3835, ThermoFisher Scientific, Waltham, MA) and bacterial inoculum ...
-
bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were plated on collagen coated plates and cultured in Dulbecco’s modified Eagle’s medium supplemented with 0.2% bovine serum albumin (fatty acid free; Fisher Scientific), 25 mM glucose ...
-
bioRxiv - Microbiology 2020Quote: ... 2.5% haematocrit) and grown in either minimal fatty acid (mFA) medium [RPMI Medium 1640 [+] L-Glutamine (Life Technologies) supplemented with 25 mM HEPES ...
-
bioRxiv - Immunology 2020Quote: ... 10μM of free fatty acids solubilized by DMSO and conjugated in 250μM BSA-lipid free RPMI (Gibco 27016021): Oleic Acid (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... we cultivated the cells in a medium with 2% fatty acid-free BSA (Life Technologies Invitrogen, Carlsbad, CA).
-
bioRxiv - Neuroscience 2023Quote: ... we cultivated the cells in a medium with 2% fatty acid-free BSA (Life Technologies Invitrogen, Carlsbad, CA).
-
bioRxiv - Developmental Biology 2021Quote: ... followed by binding to Protein-G Dynabeads (Invitrogen, 10003D) for 20 minutes hours at 4°C to enrich for endothelial cell nuclei ...
-
bioRxiv - Cell Biology 2022Quote: ... Binding of indicated antibodies to Dynabeads Protein A (Invitrogen) was performed overnight ...
-
bioRxiv - Immunology 2024Quote: ... Pierce protein G IgG binding buffer (Thermo Fisher Scientific) was used to dilute the serum 1:1 before loading as well as for equilibration and wash steps ...
-
bioRxiv - Cell Biology 2024Quote: ... Binding of indicated antibodies to Dynabeads Protein A (Invitrogen) was performed overnight ...
-
bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
-
bioRxiv - Pathology 2020Quote: ... qRT-PCR was performed in 96-well Human Fatty Liver Array plates (Thermo Fisher Scientific cat # 4391524) on a QuantStudio 3 System (Applied Biosystem ...
-
bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...
-
bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was homogenised with a Dounce homogeniser and placed in a low protein binding 15mL tube (Thermo Fisher Scientific, 30122216) containing 10mL 1X artificial CSF (pH 7.4 ...
-
bioRxiv - Cell Biology 2022Quote: ... + 1X Glutamax + 1X Sodium pyruvate + 0.1% of fatty acid-free bovine serum albumin and 1X penicillin/streptomycin – all were from GIBCO) ...
-
bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
-
bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
-
bioRxiv - Neuroscience 2021Quote: ... streptavidin biotin-binding protein (Streptavidin Alexa 488, 1:500, Invitrogen) was diluted in 5% BSA with 5% NGS and 0.3% Triton-X overnight at 4 °C ...
-
Complement-associated loss of CA2 inhibitory synapses in the demyelinated hippocampus impairs memorybioRxiv - Neuroscience 2021Quote: ... Streptavidin biotin-binding protein (Streptavidin Alexa 488, 1:500, Invitrogen) was diluted in 5% BSA with 5% NGS and 0.3% Triton-X overnight at 4°C ...