Labshake search
Citations for Thermo Fisher :
1 - 50 of 7740 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... eCC6.30.2 Fabs were purified by digesting IgG using Fab digestion kit (ThermoFisher, 44985) according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... The eCR3022.20 Fab was purified by digesting eCR3022.20 IgG using Fab digestion kit (ThermoFisher, 44985) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... The purified polyclonal IgGs were then digested into Fabs using Pierce Fab Preparation Kit (Thermo Scientific). Briefly ...
-
bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgG Fab recombinant secondary antibody (Invitrogen), anti-NKp46 (9E2 ...
-
bioRxiv - Immunology 2020Quote: 47D11 Fab was digested from IgG with papain using a Pierce Fab Preparation Kit (Thermo Fisher Scientific), following the manufacturer’s standard protocol.
-
bioRxiv - Immunology 2023Quote: Purified C68.59 IgG was digested into Fab fragments using a Pierce™ Fab Preparation Kit (Thermo Fisher) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... Fab fragments were generated and purified from IgGs using the Pierce Fab Preparation Kit (Cat# PI44985; Thermo Scientific) and used in neutralization assays at 2x molar concentration relative to IgG.
-
bioRxiv - Neuroscience 2022Quote: ... 0.5 µl of SAP (Affymetrix), 0.1 µl E ...
-
bioRxiv - Microbiology 2021Quote: Fab fragments were generated and purified from full length IgG using a Pierce™ Fab Preparation Kit (Thermo Fisher). Purified Fabs were mixed with SARS-CoV-2 S protein ...
-
bioRxiv - Immunology 2023Quote: ... Fabs were purified using CaptureSelect IgG-CH1 Affinity Matrix (Thermo Scientific).
-
bioRxiv - Microbiology 2022Quote: The 87G7 Fab fragment was digested from the IgG with papain using a Pierce Fab Preparation Kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP, Affymetrix Cat # 78390) and Sanger sequenced.
-
bioRxiv - Molecular Biology 2024Quote: ... before Fab was purified with CaptureSelect™ IgG-CH1 Affinity Matrix (ThermoFisher). Bound Fab was eluted in a buffer containing 100 mM glycine pH 3.0 ...
-
bioRxiv - Neuroscience 2022Quote: ... Then Shrimp Alkaline Phosphatase (SAP) reaction (total volume 10 µl) was performed with 10× SAP buffer (Affymetrix), 0.5 µl of SAP (Affymetrix) ...
-
bioRxiv - Cell Biology 2021Quote: ... B2-Fab was stained with biotinylated goat anti-human IgG (1:1,000, Invitrogen) followed by streptavidin-PE staining ...
-
bioRxiv - Developmental Biology 2022Quote: ... purified by Exo-Sap-IT (Affymetrix, 78201) to remove unincorporated primer ...
-
bioRxiv - Microbiology 2024Quote: ... The control Fab was prepared from the IgG form of the antibody (mAb1) using a PierceTM Fab Micro preparation kit (ThermoFisher Scientific, CAT#44685) following manufacturer instructions.
-
bioRxiv - Microbiology 2020Quote: ... CR3022 Fab and CR3022 IgG were transiently expressed in Expi293™ (ThermoFisher Scientific, UK) and proteins were purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare ...
-
bioRxiv - Immunology 2021Quote: ... and H5.31 and H5.28 Fabs were purified with CaptureSelect IgG-CH1 (Thermo Fisher Scientific). To obtain complexes of Fabs and HA head domain ...
-
bioRxiv - Microbiology 2019Quote: ... DNA was treated with Exo-SAP-IT (ThermoFisher) and incubated at 37°C for 15 min followed by a 15 min incubation at 80°C to inactivate the enzyme ...
-
bioRxiv - Genomics 2020Quote: ... with excess primers removed with EXO-SAP-IT (Affymetrix), and then amplified samples were diluted 1/5 in DNA suspension buffer ...
-
bioRxiv - Immunology 2024Quote: Fabs were papain digested using Pierce Fab Preparation Kit (Thermo Fisher) following the manufacturer’s protocol for human Fab digest ...
-
bioRxiv - Physiology 2021Quote: ... Alexa Fluor 546-conjugated goat anti-rabbit IgG (Fab)2 fragment and Alexa Fluor 488 conjugated goat anti-mouse IgG were from Invitrogen (Taastrup, Denmark). All primary antibodies were diluted in PBS with 5% normal goat serum ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... with detection of the complex with goat anti–human IgG Fab (200 µg/well; Invitrogen, #31122).
-
bioRxiv - Immunology 2022Quote: ... the Fabs were purified from the supernatant using Capture Select IgG-CH1 Affinity beads (Life Technologies) according to the manufacturer indications.
-
bioRxiv - Immunology 2022Quote: Fab fragments were generated using the Pierce Fab Preparation kit (Thermo Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: Fab fragments were prepared using the Pierce Fab Preparation Kit (Thermo Scientific) as previously described 41 ...
-
bioRxiv - Bioengineering 2024Quote: ... Trastuzumab Fab was prepared using Pierce Fab Preparation Kit (Thermo Fisher Scientific). The digestion and purification were performed following the manufacturer’s instruction ...
-
bioRxiv - Bioengineering 2022Quote: ... The PCR products were purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis (through Genewiz) ...
-
bioRxiv - Cell Biology 2021Quote: ... The PCR product was purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis ...
-
bioRxiv - Immunology 2020Quote: Purified monoclonal antibodies (Fab/IgG) were buffer exchanged into PBS using equilibrated ZebaSpin columns (Thermo Fisher Scientific). The protein concentration was determined and the antibodies biotinylated by incubating for 30 min at room temperature with EZ-Link NHS-PEG4-Biotinylation reagent (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... The IgGs and Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography.
-
bioRxiv - Immunology 2023Quote: ... The Fab domain was expressed in Expi293F cells and purified by IgG-CH1 Affinity Matrix (Thermo Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The flow-through was collected and Fabs were purified using CaptureSelectTM IgG-CH1 affinity resin (ThermoFisher Scientific). 200 mM acetic acid (pH 3.5) ...
-
bioRxiv - Immunology 2019Quote: ... Fabs were produced using mouse IgG1 Fab F(ab)2 Kits (Thermo Scientific) and verified by gel electrophoresis and Coomassie Blue staining ...
-
bioRxiv - Immunology 2021Quote: ... The IgG antibodies and Fabs were purified with a CaptureSelect™ CH1-XL Matrix column (Thermo Fisher Scientific) for affinity purification and a HiLoad Superdex 200 pg column (Cytiva ...
-
bioRxiv - Cancer Biology 2022Quote: ... Mouse antiHuman IgG Fab Secondary Antibody-PE and LAMP1 Monoclonal Antibody (LY1C6) eFluor 660 (all from Thermofisher Scientific); CellTiter 96® AQueous One Solution Assay (Promega ...
-
bioRxiv - Microbiology 2021Quote: ... The IgG antibodies and Fabs were purified with a CaptureSelect™ CH1-XL Matrix column (Thermo Fisher Scientific) for affinity purification and a HiLoad Superdex 200 pg column (Cytiva ...
-
bioRxiv - Microbiology 2024Quote: All antibodies in IgG and Fab forms in this study were transiently expressed in ExpiCHO cells (Thermo Fisher). For IgGs and Fabs ...
-
bioRxiv - Genomics 2019Quote: ... amplicons were cleaned with Exo-SAP (Affymetrix, Santa Clara, CA, USA), quantified with QuantiFluor dsDNA system (Promega ...
-
bioRxiv - Genomics 2023Quote: ... 1 ul of EXO-SAP-IT Express (Life Technologies-75001.1.ML) was added to the reaction ...
-
bioRxiv - Biochemistry 2021Quote: ... Anti-Igα Fab fragment was prepared with Pierce Fab Micro preparation kit (Thermo Scientific) using immobilized papain according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Fab fragments were prepared from SG2 using a Pierce Fab Preparation Kit (Thermo Scientific). The rabbit polyclonal antiserum ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Pierce Fab Preparation Kit (ThermoFisher) was used to generate Fab fragments of anti-Flag IgG antibody ...
-
bioRxiv - Microbiology 2020Quote: Polyclonal anti-CD55 antibody and control IgG were digested using the Pierce™ Fab preparation kit (Thermo Fisher Scientific). The resulting Fab fragments were quantified on a spectrophotometer ...
-
bioRxiv - Immunology 2020Quote: 8A7H5 and control Fabs were generated using the Pierce Fab Micro Preparation Kit (Thermo Fisher) and purified on Protein G columns (Thermo Fisher) ...
-
bioRxiv - Immunology 2019Quote: Fab preparation was performed using the Mouse IgG1 Fab preparation micro kit (Thermo Scientific #44680) and CD38 ...
-
bioRxiv - Microbiology 2019Quote: ... 2 µl of EXO-SAP (100 U shrimp alkaline phosphatase (Thermo Scientific), 100 U Exonuclease I (Thermo Scientific) ...
-
bioRxiv - Plant Biology 2021Quote: ... Amplicons were cleaned using Exo-SAP-IT (Affymetrix, Santa Clara, CA, USA), and Sanger-sequenced at the Core Genomic Facility at the University of Sheffield ...
-
bioRxiv - Molecular Biology 2022Quote: ... The amplified products were treated with Exo-Sap II (Thermo Fisher Scientific), and sequence termination reactions were performed from both directions of strands using BigDye Terminator v3.2 (Applied Biosystem) ...