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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for E3 Ubiquitin Protein Ligase RNF128 RNF128 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... or anti-ubiquitin antibodies (Invitrogen #PA1-10023) and detected with HRP conjugated IgG (Invitrogen #31460 ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were visualized using Western blot and monoclonal anti-ubiquitin antibody (Fisher scientific, eBioP4D1 (p4D1)) ...
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bioRxiv - Bioengineering 2020Quote: ... and HisTags (4E3D10H2/E3; ThermoFisher) were all used at 1:100 dilutions.
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bioRxiv - Synthetic Biology 2023Quote: ... Mouse monoclonal anti-Ubiquitin (eBioP4D1) antibody (Invitrogen, 14-6078-82), 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: SUMO1 and SUMO2 SATT plasmids containing a SUMO E3 ligase of interest were generated using Gateway® cloning LR reaction (Thermo Fisher Scientific). LR reactions were performed using a donor plasmid containing an E3 enzyme cDNA without stop codon and a SATT plasmid as destination vector ...
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bioRxiv - Immunology 2021Quote: ... larvae were transferred to feeding containers and kept in E3 embryo medium (E3) [5mM NaCl (Fisher Scientific), 0.17 mM KCl (Dot Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... anti-ubiquitin (131600, Invitrogen), 1:2000 for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... solutions of E3 + Tricaine and E3 + Tricaine + 135 mM sodium chloride were prepared and supplemented with 10µM of CoroNa Green (Invitrogen Cat#C36675). A wildtype larva was mounted in the device as described above and confocal image stacks were collected over time as the two CoroNa Green containing media were flowed into either chamber of the device ...
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bioRxiv - Cell Biology 2020Quote: ... anti-ubiquitin (#701339, Thermo Scientific). Total proteins were stained with REVERT Total Protein Stain (#926-11011 ...
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bioRxiv - Physiology 2023Quote: ... Ubiquitin (Invitrogen #1301600, 1:1000), CPT1b (Thermo #PA5-79065 ...
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bioRxiv - Neuroscience 2023Quote: ... immunoblotted with anti-ubiquitin antibody 1:500 in 5% BSA TBST (13-1600, Invitrogen) and reprobed with anti-C-cadherin antibody 1:100 in 5% non-fat milk TBST (Developmental Studies Hybridoma Bank ...
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bioRxiv - Genetics 2023Quote: ... The membrane was boiled for 30 min in water and incubated for 1 hour in 5% skim milk in TBS pH 7.4 then for 2 hours in primary antibody (1:2,000 ubiquitin monoclonal antibody 13-1600 Invitrogen) and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP ...
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bioRxiv - Cell Biology 2022Quote: ... and immunoblotted with anti-Ubiquitin (Invitrogen MAI-10035), anti-GFP (Santa Cruz SC-8334) ...
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bioRxiv - Molecular Biology 2020Quote: ... For overexpression of ubiquitin, two copies of the 6His-ubiquitin sequence (Treier et al., 1994) were inserted in pCDNA3 (Invitrogen, V79020) to generate pcDNA3-2x-6His-Ub ...
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bioRxiv - Molecular Biology 2020Quote: Approximately 4 x 105 HEK293T cells in a 6-well plate were transfected with 250 ng/well of pMT107-octa-6 His-ubiquitin or 200 ng/well of pcDNA3-2x-6His-ubiquitin constructs using 50 µl/well Opti-MEM (Gibco; 31985062) and 2 µl/well of FuGENE 6 Transfection reagent (Promega ...
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bioRxiv - Genomics 2021Quote: ... T4 ligase (Ambion, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... pET SUMO (small ubiquitin-like modifier) expression vector (Invitrogen) bearing Mpro from SARS-CoV-2 gene with N-terminal His-SUMO tag was transformed into E ...
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bioRxiv - Microbiology 2020Quote: ... 10x ligase buffer (Invitrogen), 200 mM EDTA ...
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bioRxiv - Genomics 2020Quote: ... coli DNA ligase (Invitrogen), 0.3 μl 10 U/μl E ...
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bioRxiv - Microbiology 2022Quote: ... T4 Ligase (Thermo Scientific) was used for ligation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2μL AMP Ligase (ThermoFisher), 2μL T4 DNA polymerase ...
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bioRxiv - Genomics 2022Quote: ... using BP ligase (Invitrogen). Ligations were transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants that contain the eluted targets were analyzed by immunoblotting with anti-Ub (ubiquitin, PTM-1107, PTM-BIO) and anti-RFP antibodies (MA5-15257, Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... E3 or E4 cDNA into the p-EGFP-N1 or p-mCherry-N1 vector (Invitrogen) between the XhoI and BamHI restriction sites ...
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bioRxiv - Molecular Biology 2019Quote: ... The Jpx E1-E3 isoform was cloned into pEF1/V5-His vector (Invitrogen Cat# V92020), which contains an EF-1α promoter for mammalian expression and a T7 promoter for in vitro transcription ...
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Asymmetric neurogenic commitment of retinal progenitors is regulated via the Notch endocytic pathwaybioRxiv - Developmental Biology 2020Quote: ... The E3 medium was supplemented with 100 U of penicillin and streptomycin (Thermo Fisher Scientific). Transplanted embryos were heat shocked at 24 hpf for 15 minutes at 39°C and imaged from 28 hpf.
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bioRxiv - Cell Biology 2023Quote: ... Isotonic medium was prepared by supplementing 1x Tricaine with E3 with either NaCl (Fisher Scientific) or D-Sorbitol (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... contained 40 µM human Flag-tagged ubiquitin (Fisher Scientific U-120), 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific, E304050), 2.4 µM human His6-UBE2T E2 enzyme (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... RK13+E3+K3 cells were supplemented with 500µg/ml geneticin and 300µg/ml zeocin (Life Technologies). Cell lines were seeded in 6-well (at 5 X 105 cells/well ...
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bioRxiv - Developmental Biology 2022Quote: ... Mouse Gof18 GFP-E3 epiblast stem cells (EpiSCs)148 were cultured in Stem Flex media (Gibco) on FBS-coated dishes.
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bioRxiv - Evolutionary Biology 2021Quote: ... 2.5 μL T4 Ligase (ThermoFisher), 2.5 μL Bpil (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... and T4 DNA ligase (Invitrogen). The ∼3.5kb ligation product was cloned into rapid TA cloning vector pCR8/GW/TOPO (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... T4 DNA ligase (ThermoFisher Scientific) served to ligate the purified inserts into pCAGGS/MCS plasmid (34 ...
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bioRxiv - Biophysics 2020Quote: ... T4 DNA ligase (ThermoFisher Scientific) was added ...
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bioRxiv - Cell Biology 2021Quote: ... and ligated (T4 ligase, Invitrogen) into gel purified pFA6a-TRP1-GAL1 digested with PacI/AscI (New England BioLabs) ...
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bioRxiv - Bioengineering 2022Quote: ... T4 DNA ligase (Life Technologies) was used according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... T4 DNA ligase (ThermoFisher Scientific) to blunt the 5’ and 3’ overhangs ...
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bioRxiv - Synthetic Biology 2021Quote: ... and T4 DNA Ligase (ThermoFisher). The reaction mix was transformed into chemically competent E ...
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bioRxiv - Molecular Biology 2019Quote: ... coli DNA ligase (Invitrogen, 18052019), 0.013 U RNAseH (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... coli DNA ligase (Invitrogen, 18052019), 0.013 U RNase H (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... using T4 DNA Ligase (Invitrogen). Overexpression was carried out in E ...
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bioRxiv - Microbiology 2021Quote: ... using T4 DNA ligase (Invitrogen). 8 µL of each ligation was transformed into One Shot TOP10 Chemically Competent E ...
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bioRxiv - Synthetic Biology 2023Quote: ... T7 Ligase (Thermo Fisher Scientific) and PNK (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... coli ligase (Invitrogen, Cat. # 18052019), 1.5 μL DNA polymerase I (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... and T4 DNA ligase (Invitrogen). pICSL86922OD was kindly provided by Mark Youles (The Sainsbury Laboratory ...
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bioRxiv - Cancer Biology 2023Quote: ... and T4 ligase (Thermofisher, #EL0011) at 37°C for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... and T4 DNA Ligase (Invitrogen)) into a backbone plasmid containing a 50bp homologous connector sequence to facilitate in vivo recombination of the gene cluster as described in [22] (Sup ...
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bioRxiv - Plant Biology 2021Quote: ... Antibody-coated Protein A Dynabeads (Invitrogen) were incubated 12 hours at 4 °C with the samples ...
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bioRxiv - Plant Biology 2023Quote: ... Antibody-coated Protein A Dynabeads (Invitrogen) were incubated 12 h at 4°C with the samples ...