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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Dengue Virus Serotype 2 VLP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... VLP-WT and VLP-cvD were expressed using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... VLPs were incubated for 2 hours in the DNAse and RNAse cocktails (Invitrogen, Carlsbad, CA) with the addition of 14 µL of 20 mg/ml Proteinase K (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The dengue 2 E protein antibody PA5-32246 was used at 1:10,000 for western blotting (Thermo-Fisher Scientific, Waltham, MA). Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL ...
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bioRxiv - Bioengineering 2024Quote: VLP and scFc-VLPs (4µM) were applied onto a glow-discharged carbon-supported copper TEM grid (TEM-CF200CU50, Thermo Fisher Scientific) and incubated for 60 s before gently removing the solution ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae serotype 2 strain D39 [17] was plated on brain heart infusion (BHI, Thermo Scientific, MA, USA) agar plates supplemented with 3% v/v defibrinated horse blood (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Dengue viruses were grown in C6/36 cells cultured in RPMI 1640 (Gibco 11875-093), L-Glutamine (Gibco 25030-081) ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was extracted from the lysed VLPs using TriReagent (Ambion) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... VLPs were resuspended in Life cell imaging solution (Thermo Fisher) and stored at − 80°C ...
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bioRxiv - Microbiology 2020Quote: ... VLPs were incubated with AnnexinV-PE conjugate (AnV-PE) (Invitrogen) in AnV binding buffer for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The purified VLP fraction was treated with DNase I (Ambion) at 37 °C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Pelleted VLPs were resuspended in 1X NuPAGE LDS sample buffer (ThermoFisher). Cell lysates were collected by washing cells twice with PBS followed by lysis in CytoBuster ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted VLPs were resuspended in 1X NuPAGE LDS sample buffer (ThermoFisher). Cell lysates were collected by washing cells twice with PBS followed by lysis in CytoBuster (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were buffer exchanged into DPBS (Thermo Fisher Scientific Cat #: 14190-144) using a 100-kDa Amicon filter (Sigma ...
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bioRxiv - Microbiology 2023Quote: We mixed 2 μl of EEEV VLP at a concentration of 1.6 mg ml-1 in DPBS (Thermo Fisher Scientific Cat #: 14190-144) with 2 μl of VLDLRLBD-Fc in TBS supplemented with 2 mM CaCl2 at a concentration of 1.6 mg ml-1 ...
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bioRxiv - Immunology 2019Quote: Chimeric VLPs or monomeric recombinant proteins were coated onto Maxisorp microtiter plates (Nunc) at 1μg/ml in PBS and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Enriched VLPs were lysed with 10% SDS and 20mg/mL Proteinase K (Ambion) at 56C° for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2019Quote: ... VLPs were produced using the Bac-to-Bac Baculovirus Expression System (Invitrogen, California, USA) and purified through a cesium chloride gradient as previously described (63) ...
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bioRxiv - Microbiology 2023Quote: Norovirus VLPs were labelled with an Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) dissolved in DMSO at a concentration of 10 µg/mL ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS) (Thermo Fisher Scientific #: 14190-144) using a 100-kDa Amicon filter (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated virus stocks in reduced-serum DMEM (2% FBS) supplemented with 50mM HEPES buffer (Gibco #15630080) were stored at -80°C ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were expressed with the Bac-to-Bac TOPO Expression system (Invitrogen, Catalog nos. A11101, A11100) according to instructions from the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Stained VLPs were image with Talos L120 transmission electron microscope (TEM, Thermo Fisher Scientific, former FEI) with 47000x magnification ...
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bioRxiv - Genomics 2021Quote: ... cells or virus pellet were incubated with 2 mM of EZ-Link Psoralen-PEG3-Biotin (ThermoFisher Scientific) at 37°C for 10 min in PBS containing 0.01% digitonin ...
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bioRxiv - Microbiology 2021Quote: The spike of the SARS-CoV-2 virus (D614G variant) was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the codon-optimized sequence obtained by Ou et al ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated for 2 hours with the virus and then washed twice with Neurobasal-A medium (Gibco) containing 2% B-27 (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Microbiology 2023Quote: ... l ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for l h ...
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bioRxiv - Microbiology 2024Quote: ... and 2 U/µl of M-MulV-RT (Reverse transcriptase of Moloney Murine Leukemia Virus; Thermo Scientific, USA).
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bioRxiv - Immunology 2019Quote: ... Oligonucleotide primers and a dual labelled probe for DENV 1,2,3,4 serotypes were used (Life technologies, USA) based on published sequences ...
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bioRxiv - Immunology 2020Quote: ... Oligonucleotide primers and a dual labeled probe for DENV 1,2,3,4 serotypes were used (Life technologies, India) based on published sequences [19].
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...