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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... under the control of a cytomegalovirus promoter (ViraPower Adenovirus Expression System, Invitrogen). The AAV-Tau was verified by sequencing and the production of Tau by western blot ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplicons were assembled with a 5′ cytomegalovirus (CMV) promoter amplified from pcDNA3.1 (Invitrogen) by Gibson assembly (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for surface antigens in PBS (Gibco) or Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2020Quote: Expression cassettes consisting of the doxycycline-inducible cytomegalovirus (CMV) promoter (derived from the Invitrogen T-REx system), the intron-containing EF1α 5 UTR ...
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bioRxiv - Cell Biology 2020Quote: Staining of cell surface antigens was performed with antibodies from Invitrogen-ThermoFisher except for PE-Cy5.5-streptavidin conjugate ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was performed with Low pH IHC Antigen Retrieval Solution (Invitrogen) in a pressure cooker set to 100°C for 10 minutes ...
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bioRxiv - Immunology 2023Quote: NY-BR-1 antigen positive cell line CAMA-1 A2B2M and antigen negative SKMEL28 A2B2M were stained with CellTracker™ Deep Red Dye (Invitrogen, C34565) and seeded at 15000 and 10000 cells/well ...
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... The cytomegalovirus (CMV) promoter was amplified by PCR from the pcDNA 3.1D/V5-His-TOPO plasmid (Life Technologies) with forward primer 5’-AGTAATCAATTACGGGGTCATTAGTTCAT-3’ and reverse primer 5’ CATAGGAGATGCCTAAGCCGGTGGAGCTCTGCTTATATAGACCTC-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval was performed using IHC Antigen Retrieval Solution (ThermoFisher, 00-4955-58) in a steamer for 40Dminutes ...
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bioRxiv - Biophysics 2020Quote: ... splenic antigen-presenting cells were washed with RPMI 1640 culture medium (Gibco). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... Tor1A and Tor1B coding regions were amplified from HeLa cDNA and cloned into the vector pcDNA5.1-GFP FRT/TO (cytomegalovirus [CMV] promoter; Invitrogen) for generation of stable ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were prepared using Denaturing Cell Extraction Buffer (Invitrogen) containing protease & phosphatase inhibitor cocktail (ThermoScientific) ...
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bioRxiv - Immunology 2020Quote: ... Expi293F cells for recombinant antigen and monoclonal antibody production (Thermo Fisher Scientific, A1452) were maintained at 37°C in 8% CO2 in Expi293F expression medium (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
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bioRxiv - Physiology 2022Quote: ... Targeted antigen retrieval was performed by boiling sections in 1x IHC antigen retrieval solution (Invitrogen) for 15-20 mins ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were transferred to phasemaker tubes (Invitrogen), to which 200 μl chloroform was added ...
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bioRxiv - Microbiology 2022Quote: Cell lysates were collected in TRIzol reagent (Ambion) at indicated times post-infection ...
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bioRxiv - Molecular Biology 2021Quote: ... whole cell lysates were prepared in Cell Extraction Buffer (ThermoFisher Scientific) supplemented with protease inhibitor cocktail P8340 ...
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bioRxiv - Immunology 2023Quote: ... or control antigen PE (Thermo Fisher) were mixed with adjuvants per manufacturer’s protocols and added to cells immediately after plating ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and a lab-made cytomegalovirus promoter-driven enhanced green fluorescent protein (GFP) reporter construct using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). On day three ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: The coding region of each opsin transcript was amplified from eye cDNA and subcloned in a modified pFRT-TO expression vector cassette derived from pcDNA5 and containing the human cytomegalovirus (CMV) immediate early promoter (Invitrogen, USA). The expression plasmid was modified to include a C-terminal tag by the monoclonal antibody FLAG epitope sequence (DYKDDDDK) ...
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bioRxiv - Physiology 2020Quote: ... and a non-targeting pre-miRNA under bicistronic control of the cytomegalovirus (CMV) promoter in the pcDNA6.2GW/EmGFP-miR plasmid (Invitrogen, Carlsbad, CA). The Fbxl22 RNAi plasmid encodes EmGFP and an artificial pre-miRNA targeting the full length gene of mouse Fbxl22 under bicistronic control of the CMV promoter ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lysates were sonicated (550 Sonic Dismembrator, Fisher Scientific) for 2 min (with one 10 sec pulse at amplitude 5 per 15 sec ...
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bioRxiv - Cell Biology 2021Quote: RNA from cell lysates was extracted using Trizol (Invitrogen, USA ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysates were obtained in RIPA buffer (ThermoFisher Scientific) supplemented with Complete protease inhibitor cocktail (Roche ...
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bioRxiv - Genomics 2020Quote: ... cell lysates were prepared by using RIPA buffer (Invitrogen) in the presence of Complete protease inhibitor cocktail (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: Cell lysates were denatured in sample buffer (Invitrogen, NP0007) with reducing agent (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... cell lysates were prepared with TPER buffer (Thermo Scientific). For the internalization assay ...
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bioRxiv - Physiology 2022Quote: Whole cell lysates were homogenized in RIPA buffer (Invitrogen) and protein quantified with a BCA protein assay (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Cell lysates were quantified by BCA (Thermo Fisher Scientific), analyzed on a denaturing Tris-HCl polyacrylamide gel ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were prepared using NP40 lysis buffer (ThermoFisher) containing 1% NP40 ...
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bioRxiv - Cell Biology 2023Quote: ... Clarified cell lysates were treated with RNase I (Invitrogen) to digest RNA not protected by ribosomes ...
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bioRxiv - Microbiology 2024Quote: ... Cell lysates were prepared using NP40 lysis buffer (ThermoFisher) containing 50mM Tris-Cl (pH 7.4) ...
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bioRxiv - Genomics 2023Quote: ... cell lysates were prepared with MPER buffer (ThermoFisher Scientific). For LC3B Western blots ...
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bioRxiv - Microbiology 2023Quote: ... Cell protein lysates were quantified by Bradford assay (ThermoFisher), then separated by Tris-Glycine SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared in RIPA buffer (ThermoFisher Scientific) supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Neuroscience 2024Quote: Mouse-hosted antibodies [anti-rat endothelial cell antigen (RECA-1; 1:50, MA1-81510, Invitrogen), anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2024Quote: ... brain slices underwent an additional antigen retrieval step in 1X IHC Antigen Retrieval Solution (Invitrogen, 00-4956-58) for 15 minutes at 95°C prior to blocking.
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bioRxiv - Synthetic Biology 2020Quote: Total cell lysates were prepared using NP40 cell lysis buffer (Thermo Fisher, FNN0021) containing a protease/phosphatase inhibitor cocktail (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysate was extracted from ice cold cells using RIPA (Thermo Scientific) plus Halt inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysate was collected by lysing cells with TRIzol reagent (15596018; ThermoFisher Scientific) after supernatant had been removed and cells had been washed in PBS ...
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bioRxiv - Physiology 2022Quote: ... or immunoprecipitated type I and II PKA holoenzymes from iPSC-CMs whole cell lysate or whole heart lysates from Stk25+/+ or Stk25-/- mice following the manufacturer’s protocol (EIAPKA, Invitrogen). Briefly ...
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bioRxiv - Immunology 2019Quote: ... diluted whole cell lysate and phosphoenriched lysates were processed for SDS-PAGE with NuPage LDS Sample buffer (Life Technologies) and 100mM dithriothreitol (Sigma) ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were pre-cleared using protein A beads (Invitrogen). Pre-cleared lysates were immunoprecipitated using anti-lamin B1 antibody and these immunoprecipitated samples were incubated with protein A-sepharose beads (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells lysates were reverse transcribed using Superscript™ III (Invitrogen) and pre-amplified for 20 rounds using a custom-designed set of target-specific primers ...
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bioRxiv - Microbiology 2020Quote: ... RNA from cell lysates were collected using Trizol reagent (Invitrogen). RNA was then extracted from Triazol using the Direct-zol RNA Miniprep Plus kit (Zymo Research #R2072 ...