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Citations for Thermo Fisher :
1 - 50 of 59 citations for Cyclin B1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cyclin B1 (Fisher Scientific, BDB554177), Phospho-Ser28-Histone3 (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... or CDK1/cyclin B1 (Thermo Fisher) in Phosphorylation Buffer (50 mM MOPS pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: Purified recombinant GST-tagged CDK1-cyclin A2 and His6-tagged CDK1-cyclin B1 (Invitrogen) were stored in 150 mM NaCl ...
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bioRxiv - Genetics 2023Quote: ... or anti-cyclin B1 (Thermo Fisher Scientific, PA5-85113, 1:200), DAPI (4’,6-Diamidino-2-phenylindol) ...
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bioRxiv - Cell Biology 2024Quote: ... hTERT RPE1 Cyclin B1-YFP were cultured in DMEM/F12 plus GlutaMAX (Invitrogen) supplemented with 10% heat-inactivated FBS (HyClone ...
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bioRxiv - Cell Biology 2020Quote: C2C12 myoblasts were transfected with a plasmid containing the N-terminal 88 amino acids of human cyclin B1 fused to EGFP using Lipofectamine 3000 (Invitrogen, L3000001) with the P3000 reagent according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cyclin E (Invitrogen, MA5-32358), GAPDH (GeneTex ...
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bioRxiv - Molecular Biology 2021Quote: ... Cyclin L1 (PA540668; Life Technologies), PAF1 (NB600-274 ...
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bioRxiv - Cancer Biology 2020Quote: ... and Cyclin D1 (Invitrogen-AHF0082). The antibody against β-actin (Invitrogen-MA191399 ...
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bioRxiv - Microbiology 2020Quote: ... siRNA targeting SR-B1 (Ambion) and negative control scramble RNA (Ambion ...
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bioRxiv - Cell Biology 2020Quote: ... SR-B1 (Ambion: Cat. no. s2648, s2649), p38 mitogen-activated protein kinases (p38 MAPK ...
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bioRxiv - Immunology 2021Quote: ... or rabbit anti-Lamin B1 (ThermoFisher Scientific) diluted in Duolink® antibody diluent ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies used: cyclin E1 (Invitrogen, cat# 32-1500) & cyclin E (Cell Signaling Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-cyclin A2 monoclonal antibody (Invitrogen MA1180), mouse anti-actin monoclonal antibody (Invitrogen MA1744) ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-cyclin E monoclonal antibody (Invitrogen MA514336), mouse anti-cyclin A2 monoclonal antibody (Invitrogen MA1180) ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-cyclin D1 monoclonal antibody (Invitrogen AHF0082), mouse anti-cyclin E monoclonal antibody (Invitrogen MA514336) ...
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bioRxiv - Cell Biology 2020Quote: ... and mouse anti-Lamin B1 (1:1000, Invitrogen). The secondary antibodies used were donkey anti-rabbit or anti-mouse conjugated to IRDye 800CW or 680LT ...
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bioRxiv - Molecular Biology 2022Quote: ... HCT-116 cells expressing WT HA-cyclin D1 or MT HA-cyclin D1 (T286A) were generated by transient transfection using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Then transfected colonies were selected in the presence of G418 (1000 μg/ml for HCT-116 cells) ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-CYCLIN D1 (1:50, Invitrogen, MA5-11387), Hoechst 33342 (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... cyclin E1 (Hs00180319_m1) and human RPLPO (4326314E; Applied Biosystems). Analysis was performed as previously described using the ΔΔCT method (25).
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bioRxiv - Cell Biology 2024Quote: cyclin D3 (1:1000, Thermo Fisher Scientific MA5-12717)
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bioRxiv - Cancer Biology 2021Quote: ... 1:2000 Cyclin D3 from Thermo Fisher (Waltham, MA, USA), 1:1000 Cyclin D2 from Proteintech (Rosemont ...
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bioRxiv - Cancer Biology 2019Quote: ... Cyclin D1 antibody was purchased from Invitrogen (Waltham, MA, USA). Phospho-PXN (Y31 ...
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bioRxiv - Microbiology 2020Quote: ... using an human interferon b1 assay (4331182, Thermo Fisher Scientific) and human HPRT1 endogenous control (4333768F ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μg of control rabbit IgG or HNRNPA2/B1 (Invitrogen, PA5-34939 antibodies diluted with total of 200 μl lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... kinase assays were conducted with 55 nM Cdk1:Cyclin B (Thermo Scientific) and/or 75 nM PLK-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... A cyclin-dependent kinase inhibitor 2A (CDKN2A) TaqMan probe (Thermo Fisher Scientific) was used as an internal control ...
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bioRxiv - Neuroscience 2020Quote: ... 3.5 ug of control rabbit IgG or HNRNPA2/B1 (Invitrogen, PA5-34939), HNRNPH (Bethyl Laboratories ...
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bioRxiv - Biochemistry 2021Quote: GST-tagged p107 constructs loaded on beads were phosphorylated with recombinant Cyclin A/CDK2 (Invitrogen) in 200 nM ATP KAS buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... were combined with 0.36 μg of recombinant human protein CDK1/Cyclin B (Thermo Fisher, PV3292), 1x Kinase Buffer (Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: GST-tagged p107 constructs loaded on beads were phosphorylated with recombinant cyclin B/CDK1 (Invitrogen) in 200 nM ATP KAS buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Cell Biology 2020Quote: ... and hTERT-RPE-1 Cyclin A2-eYFP54 were cultured with DMEM/F-12 + GlutaMax (Life Technologies), and BJ primary human foreskin fibroblasts (a kind gift from Bennie Lemmens ...
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bioRxiv - Cell Biology 2019Quote: ... The immunoprecipitates were then incubated with or without 270 ng purified CDK1/cyclin B (Life technologies, Part Number ...
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bioRxiv - Cancer Biology 2021Quote: Inhibition of CDK/cyclin complexes by indisulam was measured by Z’LYTE - SelectScreen Kinase Profiling Services (ThermoFisher). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... CYCLIN E1 fragments were ligated into pJET1.2/blunt using the blunt end protocol from GeneJET (Thermo Fisher). MYC was digested with NotI and EcoRI and ligated into the corresponding cloning sites of pRetroX-Tight-Pur ...
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bioRxiv - Cell Biology 2019Quote: ... and III (DE) (1.7 kb) of the Cyclin E gene 5’ flanking sequence were synthesized by GeneArt (Invitrogen) with appropriate restriction sites at both ends and subcloned into the pMA plasmid to generate pMA-P ...
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bioRxiv - Cell Biology 2019Quote: ... The gels were run in an Owl™ EasyCast™ B1 Mini Gel Electrophoresis System (Thermo Scientific). The RNA purity and quantity were assessed by loading 1.5 µl of each sample onto a NanoDrop™ 2000c Spectrophotometer and reading the A260 nm emission ...
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bioRxiv - Plant Biology 2023Quote: ... and TTN5Q70L coding sequences were amplified with B1 and B2 attachment sites for Gateway cloning (Life Technologies) using the primer TITAN5 n-ter B1 and TITAN5 stop B2 (Supplementary Table S1) ...
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bioRxiv - Biochemistry 2023Quote: ... Ten3-B0/B1 monomeric (referred to as Ten3 from now on) proteins were diluted in HBSS (ThermoFisher) and used at a concentration of 10μg/mL in final stripe assays ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcription of starfish cyclin B (sfcyclin B) mRNA was performed as previously described (20) using the mMESSAGE mMACHINE T7 kit (Invitrogen) with the original NTP concentrations ...
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bioRxiv - Cell Biology 2023Quote: ... Cyclin B14E7E cells were selected for 72 h with Nutlin-3A 5 µM and for 7 days with Geneticin (Gibco) 0.4 mg/ml ...
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bioRxiv - Genomics 2022Quote: ... An N-terminal FLAG tag and a nuclear localization signal (NLS) along with the M.CvIPI ORF were then cloned N-terminal to Lamin B1 into pENTR (Invitrogen) using Gibson cloning (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... or CDLK5 in 10 mM HEPES-NaOH pH 7.4 and 500 mM NaCl was incubated with B1 (12.5, 25, or 50 µM) in the presence of 5× SyPRO orange dye (Invitrogen). Next ...
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bioRxiv - Cell Biology 2024Quote: ... HCT116LMN(B1&B2)-AID cells were grown in McCoy’s 5A Modified Medium (#16600-082, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% FBS (#16000-044 ...
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bioRxiv - Biochemistry 2020Quote: ... were then combined into expression constructs containing MAT1-Cyclin H-CDK7 using PmeI (MssI) and SwaI (SmiI) restriction digests (FastDigest enzymes, ThermoFisher Scientific) and subsequent in-fusion reactions ...
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bioRxiv - Cell Biology 2023Quote: ... Flp-In HEK293 cells were co-transfected with the pCDNA3-TurboID-cyclin F plasmid and the pOG44 Flp-Recombinase Expression Vector (Invitrogen, V600520) for co-expression of the Flp-recombinase using Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Pre-cleared lysates were immunoprecipitated using anti-lamin B1 antibody and these immunoprecipitated samples were incubated with protein A-sepharose beads (Invitrogen) for 2h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... For knockdown experiments MIN6 cells were resuspended with 25 nM antiGABA-B1 receptor or control siRNA (Dharmacon, siGENOME) pre-mixed with 1.6 μl/ml Lipofectamine RNAiMAX (Life technologies) in Opti-MEM I medium ...
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bioRxiv - Plant Biology 2023Quote: ... The CDS of PYE and OLV were amplified from cDNA of Fe deficient Arabidopsis WT roots with primer pairs carrying B1 and B2 attachment sites (Supplemental Table S1) and transferred via Gateway cloning into pDONR207 (Invitrogen) according to the manual (BP reaction ...