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Citations for Thermo Fisher :
1 - 50 of 7213 citations for Creatine Kinase M Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 5 μg creatine kinase (Invitrogen) and 20U RNase inhibitor (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μg creatine kinase (Invitrogen) and 20U RNase inhibitor (NEB) ...
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bioRxiv - Immunology 2023Quote: ... M-IgA was purified by using Peptide M Agarose (ThermoFisher Scientific). S-IgA was purified by two-step chromatography using the Capturem His-Taged Purification Kit (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... 40 U/ml creatine phosphokinase and 4 mM creatine phosphate (Thermo Scientific), 2% (w/w ...
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bioRxiv - Biochemistry 2023Quote: ... 25 mM creatine phosphate (Invitrogen), 0.04 mM of each of the twenty amino acids ...
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bioRxiv - Biochemistry 2024Quote: ... 25 mM creatine phosphate (Invitrogen), 0.04 mM of each of the twenty amino acids ...
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bioRxiv - Physiology 2021Quote: Serum creatine kinase activity was measured as a biomarker of muscle damage using the Stanbio™ CK Liqui-UV™ Test (Thermo Fisher Scientific) on a Biotek Synergy H1 microplate reader according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 100pmol biotinylated peptides (Peptide 2.0) were bound to 8μl streptavidin M-280 Dynabeads (Thermo Fisher Scientific) for 30mins in PBS at room temperature with agitation ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µL 1 M DTT and 27.5 µL of T4 Polynucleotide Kinase (Thermo Scientific) were added to the annealed oligonucleotides and the reaction was incubated for 2 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: The inhibition of kinase activity mediated by phospholipids was determined using the following Z’-LYTE Kinase Assay Kits: Z’-LYTE Kinase Assay Kit - Ser/Thr 6 Peptide (Thermo Fisher Scientific, Cat# PV3179) used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: Kinase activity of Src variants was measured using Omnia® Y Peptide 2 Kit (ThermoFisher Scientific). The assay is based on the detection of fluorescence increase after kinase-specific substrate phosphorylation ...
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bioRxiv - Plant Biology 2019Quote: Biotinylated peptides were enriched using Dynabeads M-280 Streptavidin (Thermo Fisher Scientific). The digested peptides were resuspened with 1 ml of PBS buffer ...
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bioRxiv - Immunology 2021Quote: Sorted HSC were also cultured with blocking CD150 peptide (FCKQLKLYEQVSPPE, Auspep, 100 μg/ml) or control peptide (DLSKGSYPDHLEDGY, Auspep,100 μg/ml) (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... These Peptides (35 µM) were incubated with Dynabeads™ M-280 streptavidin (Invitrogen # 11205D) in binding buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... extracellular signal-regulated kinase kinase (MEK, Ambion: Cat. no. s11167), Akt1 (Ambion ...
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Biochemistry 2020Quote: ... phosphoglycerate kinase (PGK; Invitrogen), and glucose-6-phosphate dehydrogenase (G-6-PDH ...
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bioRxiv - Microbiology 2023Quote: ... and T4 kinase (Invitrogen), and purified on an 8% polyacrylamide/7M urea gel and eluted in buffer containing 20 mM Tris-HCl/pH 7.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Twenty micrograms of each peptide were incubated with 20 µl of pre-washed Dynabeads M-280 Streptavidin (Invitrogen) at RT for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were separated across a 2.1 m□×□5 cm C18 separating column (1.9 mm Hypersil Gold, Thermo Fisher) with a linear gradient of 4-40% CH3CN and 0.3% formic acid over 6 min ...
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bioRxiv - Biophysics 2024Quote: ... The resulting peptide-mRNA-DNA fusions were then incubated with PfUCLH3 immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in TNB blocking buffer comprised of dH2O containing 0.1 M Tris-HCl (pH 7.5, Fisher Scientific), 0.15 M NaCl (Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... using polynucleotide kinase (Thermo Scientific). Oligonucleotides were quantified spectophotometrically using calculated extinction coefficients (OligoAnalyzer tool ...
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bioRxiv - Cell Biology 2021Quote: ... α-phosphoglycerate kinase (Life Technologies), α-HA rabbit (Sigma) ...
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bioRxiv - Biophysics 2021Quote: ... and T4 polynucleotide kinase (Thermofisher). One pmol of labeled primer was mixed with one μl of ribonuclease reaction mix ...
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bioRxiv - Microbiology 2021Quote: ... and T4 polynucleotide kinase (Thermofisher) as described (Nguyen Le Minh et al. ...
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bioRxiv - Microbiology 2022Quote: ... with polynucleotide kinase (Thermo Scientific). Duplex DNA was obtained by annealing equimolar amount of complementary ssDNA in 10mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... After blocking with blocking buffers (Thermo Scientific) for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... 1 nmol of biotinylated 4-heptad CTD peptides (PeptideSynthetics) were incubated with 0.5 mg of Streptavidin-coupled magnetic Dynabeads M-280 (Invitrogen) in 300 µL of buffer A (25mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The peptide–mRNA fusions were then incubated with human and yeast DUBm-immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: 250 μL K92 peptide at 1 mg/mL was reduced with 6 μL of 0.5 M Dithiothreitol (Thermo Fisher) for 40 minutes at 37 °C and alkylated with addition of 18 μL of 2-Iodoacetamide (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were identified using an ACQUITY M-Class UHPLC system (Waters) connected to an Orbitrap Elite mass spectrometer (ThermoFisher). Approximately 1 µg of samples was injected onto an ACUITY UPCL M-Class Symmetry C18 Trap Column ...
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bioRxiv - Biophysics 2024Quote: ... the sample was transferred into a tube previously passivated with denatured BSA blocking buffer and mixed with magnetic beads (Dynabeads M-270 Streptavidin, Invitrogen) coated with DNA strands complementary to a section of the purification linker handles on the triangles ...
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bioRxiv - Cell Biology 2021Quote: Kinase activity assays were performed using the SelectScreen biochemical kinase profiling service provided by Life Technologies. Adapta screening protocols were used to measure PIK3C2A (PtdIns3K-C2α) ...
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bioRxiv - Microbiology 2019Quote: ... using T4 polynucleotide kinase (Thermo Scientific). A 10 μl nuclease reaction mixture contained 20 μM wild-type Mmp-aCPSF1 protein or its catalytic inactive mutant ...
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bioRxiv - Molecular Biology 2023Quote: ... and T4 polynucleotide kinase (Thermo Scientific) followed by dephosphorylation using FastAP thermosensitive alkaline phosphatase from Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... 100 units T4 kinase (Life Technologies) was added and incubated for 2 hours at 37°C for phosphorylation ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... using T4 Polynucleotide kinase (Thermofisher, #EK0031) (see Supplementary figures for a list of oligonucleotides used) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were stained overnight with the following primary antibodies diluted in blocking buffer: mouse anti-neurofilament-M (1:1500, RMO270, Invitrogen, RRID:AB_2315286); rabbit anti-6xHisTag (1:2000 ...
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bioRxiv - Biochemistry 2020Quote: ... the theoretical m/z values for all peptides were used to interrogate the raw data using the Quan Browser software program (Thermo Scientific) (Table 1) ...
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bioRxiv - Biochemistry 2022Quote: ... Synthetic peptides were coupled to rabbit serum albumin (RSA) with the linker Sulfo-m-maleimidobenzoyl-N-hydroxysuccinimide ester (Sulfo-MBS, Thermo Scientific) in a carrier (1):linker (50):peptide (50 ...
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bioRxiv - Microbiology 2020Quote: ... treated with T4 polynucleotide kinase (Thermo Fisher), and cloned into the AarI (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... phosphorylated using T4 polynucleotide kinase (Thermo Scientific) for 1 h at 37°C and annealed to phagemid ssDNA (90°C for 3 min ...
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bioRxiv - Plant Biology 2020Quote: ... and Polynucleotide Kinase (Thermo Fisher Scientific, USA). Additionally ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-phosphoglycerate kinase Pgk1 (459,250; Invitrogen) antibodies were employed to detect H3 histone ...
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bioRxiv - Molecular Biology 2021Quote: ... using T4 Polynucleotide Kinase (Thermo Fisher Scientific) before gel loading.
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bioRxiv - Systems Biology 2023Quote: ... 2.5 µL T4-polynucleotide kinase (Thermo Scientific), 2 µL SUPERase•In RNase Inhibitor and incubated for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 µl T4-Polynukleotid-Kinase (ThermoFisher, EK0031) and 6.5 µl upH2O was mixed ...
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bioRxiv - Molecular Biology 2023Quote: ... and T4 polynucleotide kinase (Thermo Scientific, EK0031). M-MuLV Reverse Transcriptase (NEB ...