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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Chemokine C X C Motif Ligand 3 CXCL3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... tumor necrosis factor alpha (TNFα) and C-C motif chemokine ligand 2 (CCL2) concentrations were evaluated using the Ready-SET-Go!™ ELISA kits (ThermoFisher Scientific). CCL24 concentrations were evaluated using the Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Synthetic Biology 2023Quote: ... untreated tissue culture plastic was incubated with purified GFP ligand or c-Myc Monoclonal Antibody (Invitrogen 9E10.3, Catalog # AHO0062) in PBS for one hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... C - 3 days (Neurobasal media (Gibco) + N2 supplement (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... The protein denaturation was monitored by obtaining the fluorescence signal by increasing the temperature from 22 °C-95 °C at 0.5 °C/minute rate using QuantStudio 3 real-time PCR (ThermoFisher). The melting temperature (Tm ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25 ...
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bioRxiv - Cell Biology 2021Quote: ... hRobo1-FL-3xHA(C)/pcDNA3 or hRobo1ΔCC0-3-3xHA(C)/pcDNA3 using Lipofectamine2000 (Thermo Fisher Scientific) and incubated for 24 hours and then sub-cultured into 12-well culture dishes and allowed to bed down overnight ...
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bioRxiv - Microbiology 2023Quote: ... and 40 cycles of PCR (95°C for 3 seconds, 60°C for 30 seconds) on a QuantStudio 3 (Applied Biosystems, MA).
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bioRxiv - Microbiology 2022Quote: ... connected to a PepMap C-18 trap-column (0.075 x 50 mm, 3 m particle size, 100 pore size; Thermo Fisher) and an in-house-packed C18 column μ (column material ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged for 25 mins at 35,000 x g and the supernatant was adjusted to approximately 3 mg/ml protein (Nanodrop 2000 C, Thermofisher Scientific). To capture the SVs for content detection ...
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bioRxiv - Molecular Biology 2024Quote: ... The enteroids were then pelleted at 300 x g for 3 min at 4 °C and washed once with advanced DMEM/F-12 basal media (Gibco). The enteroid pellet was resuspended in the desired volume of IntestiCult complete medium (Stemcell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... then sections were blocked with 3% milk in 0.25% TBS-X (Triton X-100) for 0.5 h followed by incubation at 4°C overnight with biotinylated AT8 antibody (Thermo Scientific, MN1020B, 1:500). The next day ...
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bioRxiv - Cancer Biology 2022Quote: ... washed with PBS and pelleted by centrifugation (450 x g, 10 min, 4 °C) prior to being stored at -80 °C in TRIzol Reagent (Invitrogen). RNA was extracted with the Direct-zol RNA Microprep kit as per manufacturer’s instructions (Zymo Research R2062) ...
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bioRxiv - Cell Biology 2020Quote: ... HPLC analysis was performed employing a C30 reverse-phase column (Thermo Acclaim C30, 2.1 x 250 mm, 3 µm, operated at 40° C; Thermo Fisher Scientific) connected to an HP 1100 series HPLC (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... To revive hiPSCs from cryopreservation cells were thawed by incubation for 2 minutes at 37°C and retrieved by centrifugation (200 x g for 3 minutes) prior to resuspension in Essential 8 (E8, (A1517001, Life Technologies) medium with 1’RevitaCell ...
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bioRxiv - Physiology 2023Quote: ... UHPLC analysis was performed on a C30 reverse-phase column (Thermo Acclaim C30, 2.1 x 250 mm, 3 μm operated at 55° C; Thermo Fisher Scientific) connected to a Dionex UltiMate 3000 HPLC system and a QExactive orbitrap MS (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... and mixed with 3 µ L of DMRIE-C (Invitrogen). After incubation at room temperature for 45 min ...
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bioRxiv - Developmental Biology 2022Quote: ... and then incubated at 37 °C overnight in a freshly prepared X-gal solution containing an X-gal substrate (UltraPure X-Gal, Invitrogen), 2mM MgCl2 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with primary antibodies (in 0.01 M PBS with 1% BSA and 0.3% Triton X-100) overnight at 4 °C and subsequently with corresponding secondary antibodies (1:1,000, Alexa-Fluor-conjugated, Invitrogen). DAPI was used to label the nuclei and sections were mounted with 75% glycerol ...
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bioRxiv - Microbiology 2022Quote: ... 95°C 2 min, (95°C 15 sec., 60°C 20 sec.) x 40 cycles on a StepOnePlus Real-Time PCR system (Applied Biosystems). For a standard curve ...
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bioRxiv - Molecular Biology 2021Quote: For tracking experiments cells were labelled with 5 nM JF549-HaloTag ligand for 15-30 minutes at 37 °C in mouse ES imaging medium (FluoroBrite DMEM (ThermoFisher, 10 % FCS supplemented with nonessential amino acids ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-chlamydial MreB antibody (custom anti-peptide antibody directed against the C-terminus of C. trachomatis serovar L2 MreB; ThermoFisher), or rabbit anti-six histidine tag (Genscript ...
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bioRxiv - Microbiology 2019Quote: ... rabbit anti-MreBCT antibody (custom anti-peptide antibody directed against the C-terminus of C. trachomatis serovar L2 MreB; ThermoFisher), rabbit anti-Hsp60 (kind gift of Dr ...
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bioRxiv - Cell Biology 2024Quote: ... Immunofluorescence (IF) staining was performed with antibodies for prosurfactant protein-C (proSP-C) (Invitrogen, PA5-71680), NKX2.1 (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... primary antibodies: anti-c-Myc (Invitrogen, MA1-980), anti-HA (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... at 4°C for 1-3 days followed by secondary antibody (1:500, Thermo Fisher Scientific #A-21206) overnight at 4°C.
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bioRxiv - Cell Biology 2023Quote: ... Fms-like tyrosine kinase 3 ligand (Flt3- ligand; 100 ng/mL) and 1% penicillin-streptomycin (GIBCO, Grand Island, NY, USA), in the presence or absence of G-CSF at a dose of 100 ng/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... 72°C - 3 min) then cloned into Topo PCR 2.1 (Invitrogen) and sequenced ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with primary antibodies (overnight, 4°C), washed (5X, PBS, 4°C) and incubated with species-specific secondary antibodies coupled to fluorophores (Thermo Fisher). Cells were then washed (5X ...
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bioRxiv - Neuroscience 2020Quote: ... in 3% NGS and 0.5% Triton-X for 44 h at 4°C and then in goat anti-mouse secondary antibody Alexa 488 (1:500; Invitrogen) in 3% NGS and 0.5% Triton-X for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed 3 times for 20 minutes each time in 1x PBS/0.3% Triton X-100/10% NDS at room temperature and incubated overnight at 4°C with secondary antibodies (Thermo Fisher Scientific Cat# A-21206 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.25% triton X-100 and 1XTBS at 4°C followed by immunostaining using goat Alexafluor-labeled secondary antibodies (Thermo Fisher Scientific or Jackson ImmunoResarch Laboraties ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were incubated with secondary antibody for 3 nights at 4°C with secondary antibodies at 1:500: goat anti-rabbit Alexa Fluor 488 (ThermoFisher #A-11008) and goat anti-mouse Alexa Fluor 647 (ThermoFisher #A-21236) ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were injected into a C-18 trap column (Acclaim PepMap100, 75 μm i. d. x 2 cm, C18, 3 μm, 100 Å, Thermo Fisher Scientific) and further separated on a C-18 analytical column (Acclaim PepMap100 ...
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bioRxiv - Molecular Biology 2021Quote: ... and C-terminal foldon trimerization motif followed by an octa-histidine tag was cloned into a pcDNA3.1 expression vector (Invitrogen). Further ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Immunology 2022Quote: ... and C-terminal foldon trimerization motif followed by hexa-His tag) were used to transiently transfect Expi293F cells (Gibco). Four days after transfection ...
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bioRxiv - Plant Biology 2019Quote: ... 40 cycles of 95°C for 3 s and 60°C for 30 s was run in a StepOne Real-Time PCR machine (Applied Biosystems). The quality of the graphs ...
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bioRxiv - Genetics 2020Quote: ... 30 s at 60°C and 60 s at 72°C before a final elongation step at 72 °C for 3 min by using a GeneAmp 9700 PCR System (Applied Biosystems). After PCR ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was kept after spinning at 800 rcf for 2’ at +4°C and were incubated overnight at +4°C with 3µg of dedicated antibodies: Nr2f1 antibody (Thermo Fisher PA5-21611) and GFP antibody (Abcam ab13970 ...
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bioRxiv - Plant Biology 2019Quote: ... or chicken anti-c-Myc primary antibody (A2128, Invitrogen), rabbit anti-chicken IgY HRP conjugate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2 ug c-FOS antibodies (Invitrogen, MA5-15055) were used in each ChIP reaction ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-c-Myc antibody (ThermoFisher Scientific 13-2500) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was removed from the antibody by boiling at 100 °C for 5 min in 2 X LDS Sample buffer (Life Technologies). An equal volume of each sample and its corresponding input was loaded onto a gel for electrophoresis and western blot analysis.
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bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBS-0.5%Triton X-100 at RT before overnight incubation at 4°C with Alexa555-conjugated donkey-anti-rabbit antibody (Invitrogen, #A32794) in PBS containing 5% NHS ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% Triton X-100 in PBS) and incubated overnight (4 °C) with primary antibodies against TH (OPA1-04050 1:1000, Thermo Scientific) and HA (3F10 1:200 ...
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bioRxiv - Microbiology 2020Quote: ... at 4 °C for 3 h on a Labquake rotator (ThermoFisher Scientific). The beads were washed with 1 mL cold lysis/binding buffer four times at 300 g for 4 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... for 1h at 37°C and then with 3 µM DAPI (Invitrogen) for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... 60L°C/60Lseconds), and melt curve (95L°C/15Lseconds, 60L°C/60Lseconds 95L°C/15Lseconds) on a QuantStudio 7 Flex (ThermoFisher). Messenger RNA (mRNA ...
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bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...