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1 - 50 of 6415 citations for CD3E Human HEK 293 mFc since 2019

• Homogeneity of antibody-drug conjugates critically impacts the therapeutic efficacy in brain tumors

  Yasuaki Anami, et al., bioRxiv - Bioengineering 2021

  Quote: ... free style HEK-293 human embryonic kidney cells (Invitrogen) were transfected with a mammalian expression vector encoding for the human IgG1 kappa light chain and full-length heavy chain sequences (based on variable sequences of cetuximab ...
• The increased hinge flexibility of an IgG1-IgG3 hybrid monoclonal enhances Fc-mediated protection against group A streptococci

  Arman Izadi, et al., bioRxiv - Immunology 2023

  Quote: 6.5 million human embryonic kidney (HEK) 293 cells (Gibco) were seeded the day before transfection in 150mm dishes in DMEM (Gibco ...
• Functional development of a V3-specific broadly neutralizing antibody isolated from a case of HIV superinfection

  Mackenzie M. Shipley, et al., bioRxiv - Microbiology 2021

  Quote: ... Human embryonic kidney (HEK) 293-F cells (RRID:CVCL_D603; Thermo Fisher Scientific ...
• A CRISPR-Cas9 screen reveals a role for WD repeat-containing protein 81 (WDR81) in the entry of late penetrating viruses

  Anthony J. Snyder, Andrew T. Abad, Pranav Danthi, bioRxiv - Microbiology 2021

  Quote: ... Human embryonic kidney (HEK) 293 FT cells (Thermo Fisher Scientific) were grown at 37°C in DMEM (Gibco ...
• Exon junction complex-associated multi-adapter RNPS1 nucleates splicing regulatory complexes to maintain transcriptome surveillance

  Lena P. Schlautmann, et al., bioRxiv - Molecular Biology 2021

  Quote: Flp-In-T-REx-293 (HEK 293; human, female, embryonic kidney, epithelial; Thermo Fisher Scientific, RRID:CVCL_U427), HeLa Flp-In-T-REx (HeLa FT ...
• A chimeric Japanese encephalitis vaccine protects against lethal yellow fever virus infection without inducing neutralizing antibodies

  Niraj Mishra, et al., bioRxiv - Microbiology 2019

  Quote: ... HEK 293 (human embryonic kidney 293 cells; ATCC CRL-1573) cells were cultured in DMEM (Gibco, Belgium), containing 10% FCS and 100 units/ml penicillin-streptomycin solution (Pen/Strep ...
• Potent SARS-CoV-2 Neutralizing Antibodies Directed Against Spike N-Terminal Domain Target a Single Supersite

  Gabriele Cerutti, et al., bioRxiv - Biochemistry 2021

  Quote: ... They were expressed in Human Embryonic Kidney (HEK) 293 Freestyle cells (Invitrogen) in suspension culture using serum-free media (Invitrogen ...
• Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus

  Fangzhu Zhao, et al., bioRxiv - Immunology 2021

  Quote: ... FreeStyle HEK 293 cells (ThermoFisher) were used for recombinant Env protein production ...
• Proteomic and functional mapping of cardiac Na_V1.5 channel phosphorylation reveals multisite regulation of surface expression and gating

  Maxime Lorenzini, et al., bioRxiv - Physiology 2020

  Quote: Human Embryonic Kidney 293 (HEK-293) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum ...
• A distinct pool of Nav1.5 channels at the lateral membrane of murine ventricular cardiomyocytes

  Jean-Sébastien Rougier, et al., bioRxiv - Physiology 2019

  Quote: Human embryonic kidney (HEK-293) cells were cultured in DMEM (Gibco, Basel, Switzerland) supplemented with 10% FBS ...
• Synaptogenic activity of the axon guidance molecule Robo2 is critical for hippocampal circuit function

  Heike Blockus, et al., bioRxiv - Neuroscience 2019

  Quote: ... All proteins were expressed in Human Embryonic Kidney (HEK) 293 Freestyle cells (Invitrogen) in suspension culture using serum-free media ...
• Knockdown of the TRPM4 channel alters cardiac electrophysiology and hemodynamics in a sex- and age-dependent manner in mice

  Prakash Arullampalam, et al., bioRxiv - Physiology 2022

  Quote: Human embryonic kidney (HEK-293) cells were cultured in DMEM (Gibco, Basel, Switzerland) supplemented with 10% FBS ...
• Stimulation of cardiac fibroblast Piezo1 channels opposes myofibroblast differentiation and induces IL-6 secretion via Ca²⁺-mediated p38 MAP kinase activation

  Nicola M. Blythe, et al., bioRxiv - Cell Biology 2019

  Quote: ... HEK T-REx-293 cells (Invitrogen) were transfected with pcDNA4/TO-mPiezo1 using Lipofectamine 2000 (Invitrogen ...
• ATP1A1-linked diseases require a malfunctioning protein product from one allele

  Kerri Spontarelli, et al., bioRxiv - Physiology 2023

  Quote: Human embryonic kidney cells (HEK 293) were grown in Dulbecco’s modified Eagle’s medium (Gibco) with 10% v/v fetal bovine serum (Gibco ...
• Principles of human pre-60S biogenesis

  Arnaud Vanden Broeck, Sebastian Klinge, bioRxiv - Biochemistry 2023

  Quote: ... Cas9 + sgRNA) were reverse transfected into human HEK 293-F cells (ThermoFisher Scientific, R79007) using Lipofectamine 3000 reagent (ThermoFisher Scientific ...
• CSF plasma cell expansion in LGI1-/CASPR2-autoimmune encephalitis is associated with loss of regulatory MAIT cells

  Daniela Esser, et al., bioRxiv - Neuroscience 2023

  Quote: ... Human Embryonic Kidney (HEK-293) cells were transfected with Lipofectamine 2000 (Invitrogen 11668-019) and 16 μg DNA per vector (light and heavy chains) ...
• ECD of PepT1 interacts with TM1 to facilitate substrate transport

  Jiemin Shen, et al., bioRxiv - Biochemistry 2022

  Quote: The HEK 293S cells in FreeStyle 293 media (Invitrogen/Thermo Fisher) supplemented with 2% fetal bovine serum (FBS ...
• ECD of PepT1 interacts with TM1 to facilitate substrate transport

  Jiemin Shen, et al., bioRxiv - Biochemistry 2022

  Quote: The HEK 293S cells in FreeStyle 293 media (Invitrogen/Thermo Fisher) supplemented with 2% fetal bovine serum (FBS ...
• Transmembrane helices mediate the formation of a stable ternary complex of cyt b₅ reductase, cyt b₅, and SCD1

  Jiemin Shen, Gang Wu, Ah-Lim Tsai, Ming Zhou, bioRxiv - Biochemistry 2021

  Quote: The HEK 293S cells in FreeStyle 293 media (Invitrogen/Thermo Fisher) supplemented with 2% fetal bovine serum (FBS ...
• Transmembrane helices mediate the formation of a stable ternary complex of cyt b₅ reductase, cyt b₅, and SCD1

  Jiemin Shen, Gang Wu, Ah-Lim Tsai, Ming Zhou, bioRxiv - Biochemistry 2021

  Quote: The HEK 293S cells in FreeStyle 293 media (Invitrogen/Thermo Fisher) supplemented with 2% fetal bovine serum (FBS ...
• Enhanced Complex Stability and Optimal JAK Geometry are Pivotal for a Potent Type III Interferon Response

  Theint Aung, Curt M. Horvath, Juan L. Mendoza, bioRxiv - Cell Biology 2022

  Quote: ... and HEK 293 cells in FreeStyle™ 293 Expression Medium (Gibco) were purchased from Thermo Fisher and maintained in their respective recommended media ...
• Antibody avidity and multi-specificity combined to confer protection against SARS-CoV-2 and resilience against viral escape

  Clare Burn Aschner, et al., bioRxiv - Immunology 2022

  Quote: ... HEK 293F and HEK 293S cells (Thermo Fisher Scientific, Waltham, MA) were cultured in Freestyle 293 Expression Medium (Thermo Fisher Scientific ...
• Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis

  Natalia G. Herrera, et al., bioRxiv - Biochemistry 2020

  Quote: HEK 293 suspension cells were cultured in HEK Freestyle Media (Invitrogen) at 37 C in a humidified shaking platform incubator (Kuhner ...
• Nucleus translocation of tRNA synthetase mediates late integrated stress response

  Na Wei, et al., bioRxiv - Molecular Biology 2020

  Quote: ... HEK-293 were grown in DMEM (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
• Structural inventory of native ribosomal ABCE1-43S pre-initiation complexes

  Hanna Kratzat, et al., bioRxiv - Molecular Biology 2020

  Quote: ... HEK Flp-In 293 T-Rex (Invitrogen) were grown in a 10 cm cell-culture dish to approximately 70% confluency and transfected with 0.5 µg of a pcDNA5/FRT/TO vector containing RIOK1 or RIOK1-D324A and 4.5 µg pOG44 (Invitrogen) ...
• Liver humanized NSG-PiZ mice support the study of chronic hepatitis B virus infection and antiviral therapies

  Rossana Colón-Thillet, et al., bioRxiv - Microbiology 2022

  Quote: Human embryonic kidney (HEK) 293 cells 38 were grown in DMEM (Thermo Fisher, Waltham, MA) supplemented with 10% fetal calf serum.
• TLR4 regulation in human fetal membranes as an explicative mechanism of a pathological preterm case

  Corinne Belville, et al., bioRxiv - Cell Biology 2021

  Quote: ... Human embryonic kidney (HEK) 293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10 % fetal bovine serum (FBS ...
• EZH2 specifically regulates ISL1 during embryonic urinary tract formation

  Enrico Mingardo, et al., bioRxiv - Genetics 2023

  Quote: Human embryonic kidney cells (HEK 293) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher) containing 10 % fetal bovine serum (FBS ...
• Transmembrane helices mediate the formation of a stable ternary complex of cyt b₅ reductase, cyt b₅, and SCD1

  Jiemin Shen, Gang Wu, Ah-Lim Tsai, Ming Zhou, bioRxiv - Biochemistry 2021

  Quote: ... HEK 293 cells were maintained in FreeStyle 293 media (Invitrogen/Thermo Fisher) supplemented with 2% FBS (Sigma ...
• Transmembrane helices mediate the formation of a stable ternary complex of cyt b₅ reductase, cyt b₅, and SCD1

  Jiemin Shen, Gang Wu, Ah-Lim Tsai, Ming Zhou, bioRxiv - Biochemistry 2021

  Quote: ... HEK 293 cells were maintained in FreeStyle 293 media (Invitrogen/Thermo Fisher) supplemented with 2% FBS (Sigma ...
• SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle production

  Rebekah C. Gullberg, Judith Frydman, bioRxiv - Molecular Biology 2022

  Quote: VLPs were produced in adherent HEK-293 [HEK-293](ATCC-CRL-1573) or the suspension Expi293 cells Expi293 (Gibco). HEK-293 cells were maintained in 10% fetal bovine serum (Atlanta Biologicals ...
• Efficient target cleavage by Type V Cas12a effector programmed with split CRISPR RNA

  Regina Tkach, et al., bioRxiv - Molecular Biology 2020

  Quote: ... we introduced the hAsCas12a gene into the human HEK 293 T-REx cell line nuclear genome (Flp-In ™ T-REx ™ −293 Cell Line, Invitrogen) allowing controlled expression by the tet-on system ...
• The ADAR1 editome reveals drivers of editing-specificity for ADAR1-isoforms

  Renata Kleinova, et al., bioRxiv - Genomics 2021

  Quote: Human embryonic kidney 293 cells (HEK 293) were maintained in high-glucose Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, Waltham, Massachusetts) supplied with 10% fetal bovine serum ...
• Phosphodiesterase Type 4 anchoring regulates cAMP signaling to Popeye domain-containing proteins

  Amy J Tibbo, et al., bioRxiv - Biochemistry 2020

  Quote: ... Plasmid DNA was transiently transfected into HEK-293 and HEK-293 PDE4A4 Stables using Lipofectamine LTX Reagent (Thermo Fisher Scientific) using manufacturers protocol ...
• Spironolactone but not Eplerenone Exacerbates Cisplatin Nephrotoxicity

  Gabriel R. Estrela, et al., bioRxiv - Physiology 2019

  Quote: Human embryonic kidney (HEK) 293 cells were cultured in Dubelcco’s modified Eagle’s medium (DMEM; 31966, ThermoFisher), 10% fetal bovine serum (FBS ...
• Engineered cytokine/antibody fusion proteins improve delivery of IL-2 to pro-inflammatory cells and promote antitumor activity

  Elissa K. Leonard, et al., bioRxiv - Bioengineering 2023

  Quote: Human embryonic kidney (HEK) 293F cells were cultivated in Freestyle 293 Expression Medium (Thermo Life Technologies), supplemented with 10 U/mL penicillin-streptomycin (Gibco) ...
• Modulation of Recombinant Human T-type calcium Channels by Δ⁹-tetrahydrocannabinolic acid in vitro

  Somayeh Mirlohi, et al., bioRxiv - Pharmacology and Toxicology 2020

  Quote: Flp-In T-REx 293 HEK cells (ThermoFisher) were stably transfected with pcDNA5/FRT/TO vector encoding human CaV3.1 (NM 018896.4) ...
• Oncogenic EFNA4 amplification promotes lung adenocarcinoma lymph node metastasis

  Xiangyu Zhao, et al., bioRxiv - Cancer Biology 2022

  Quote: ... HEK-293 cell was cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
• Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

  Markus Höpfler, et al., bioRxiv - Molecular Biology 2023

  Quote: HEK 293 Flp-In T-REx cells (Invitrogen) were maintained in DMEM with GlutaMAX and 4.5 g/l glucose (Gibco ...
• DNA methylation insulates genic regions from CTCF loops near nuclear speckles

  Shelby A. Roseman, et al., bioRxiv - Genomics 2023

  Quote: ... HEK 293 T was obtained from Life Technologies. HCT116 was obtained as a gift from M ...
• P66Shc Mediates SUMO2-induced Endothelial Dysfunction

  Jitendra Kumar, et al., bioRxiv - Cell Biology 2024

  Quote: ... HEK 293 cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Thermo) ...
• A versatile, chemically-controlled DNA binding switch enables temporal modulation of Cas9-based effectors

  Cindy T. Wei, et al., bioRxiv - Molecular Biology 2022

  Quote: HEK-293T cells (ATCC, CRL-3216) and HEK-293 TREx FlpIn cells (ThermoFisher) were cultured in high glucose Dulbecco’s Modified Eagle Medium (DMEM ...
• Modular and programmable RNA sensing using ADAR editing in living cells

  K. Eerik Kaseniit, et al., bioRxiv - Bioengineering 2022

  Quote: Flp-In T-REx Human Embryonic Kidney (HEK) 293 cells were purchased from Thermo Scientific (catalog #R78007). Cells were cultured in a humidity-controlled incubator under standard culture conditions (37°C with 5% CO2 ...
• Large-scale nuclear remodeling and transcriptional deregulation occur on both derivative chromosomes after Mantle cell lymphoma chromosomal translocation

  Fatimata Bintou Sall, et al., bioRxiv - Cancer Biology 2020

  Quote: Human Embryonic Kidney (HEK-293) cells were cultivated in DMEM (Gibco®, Thermo Scientific, Carlsbad, CA, USA) supplemented with 10% FBS,2mM glutamine and 1% penicillin/streptomycin.
• Large-scale nuclear remodeling and transcriptional deregulation occur on both derivative chromosomes after Mantle cell lymphoma chromosomal translocation

  Fatimata Bintou Sall, et al., bioRxiv - Cancer Biology 2020

  Quote: Human Embryonic Kidney (HEK-293) cells were cultivated in DMEM (Gibco®, Thermo Scientific, Carlsbad, CA, USA) supplemented with 10% FBS,2mM glutamine and 1% penicillin/streptomycin.
• A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment

  Lindsay B Alcaraz, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Human embryonic kidney 293 (HEK-293T) cells were stably transfected with the plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
• Development of antibody-dependent cell cytotoxicity function in HIV-1 antibodies

  Laura E. Doepker, et al., bioRxiv - Immunology 2020

  Quote: HEK 293-F cells (RRID:CVCL_D603; originally derived from female human embryonic kidney cells) were obtained from Invitrogen (Thermo Fisher Scientific ...
• Proteinase-Activated Receptor 4 (PAR4) Activation Triggers Cell Membrane Blebbing through RhoA and β-arrestin

  Christina MG Vanderboor, et al., bioRxiv - Pharmacology and Toxicology 2019

  Quote: HEK-293 (ATTC) cells were maintained in DMEM (Gibco) with 10% fetal bovine serum (Gibco) ...
• A ROS-dependent mechanism to drive progression through S phase

  Dilyana Georgieva Kirova, et al., bioRxiv - Cell Biology 2022

  Quote: ... HEK-293 (RRID:CVCL_0045) cells were grown in DMEM (Gibco) supplemented with 10% (v/v ...
• Mapping the dynamic cell surface interactome of high-density lipoprotein reveals Aminopeptidase N as modulator of its endothelial uptake

  Kathrin Frey, et al., bioRxiv - Systems Biology 2023

  Quote: ... were transfected into HEK-293 T cells (ThermoFisher Scientific) with 1:3 DNA ...