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Citations for Thermo Fisher :
1 - 50 of 620 citations for Borrelia burgdorferi sensu stricto B31 Surface Lipoprotein P27 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... burgdorferi B31 genomic DNA with Taq polymerase and TA cloned into pCR2.1 (Thermo Fisher). Constructs were sequenced to guarantee sequence fidelity.
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bioRxiv - Microbiology 2020Quote: ... Borrelia burgdorferi genomic equivalents were calculated using an Applied Biosystems 7500 Real-Time PCR system (ThermoFisher Scientific) in conjunction with PowerUp SYBR Green Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... burgdorferi antibody (Invitrogen, USA ...
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bioRxiv - Immunology 2020Quote: ... burgdorferi polyclonal IgG (Invitrogen; CAT# PA1-73007 ...
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bioRxiv - Microbiology 2022Quote: ... burgdorferi monoclonal antibody (ThermoFisher, F21l) diluted in 1% normal goat serum (NGS ...
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bioRxiv - Microbiology 2022Quote: ... burgdorferi cells with TRIzol Reagent (Invitrogen, 15596026) according to the manufacturer’s instructions after a 30-minute 3,000 x g swinging-bucket centrifugation at room temperature ...
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bioRxiv - Immunology 2020Quote: ... burgdorferi polyclonal IgG (Invitrogen; CAT# PA1-73007; Thermo-Fisher Scientific, Waltham, MA) at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... burgdorferi was purified using a GeneJET PCR Purification Kit (Thermo Scientific, #K0701), then sent for Eurofins sequencing and analyzed using the NCBI BLAST program (https://blast.ncbi.nlm.nih.gov/) ...
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bioRxiv - Cell Biology 2021Quote: ... and supplemented with 5% lipoprotein-deficient serum FCS (Life Technologies), and 1% non-essential amino acids (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... The LDL uptake assay (Image-iT Low Density Lipoprotein Uptake kit, ThermoFisher) was performed as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Larger fluorescently tagged fragments >60 bp were generated by PCR amplification of the target sequences from B31 genomic DNA and subsequent cloning into the pCR 2.1 TA vector (Invitrogen) following TOPO TA protocols ...
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bioRxiv - Molecular Biology 2022Quote: Silencer select p27/CDKN1B siRNA (s2837 cat# 4390824) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... anti-SIVmac251 Gag p27 mAb (ABL) manually conjugated with Alexa 647 (Life Technologies), anti-SIVmac251 Nef mAb (clone 17 ...
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bioRxiv - Microbiology 2022Quote: ... then stained with a Borrelia polyclonal antibody diluted to 1:50 in 1x PBS (ThermoFisher, Waltham, MA, USA; FITC-PA1-73005) according to a previously established protocol [31] ...
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bioRxiv - Microbiology 2022Quote: ... Individual samples were then screened using a duplex quantitative PCR for Borrelia burgdorferi and Borrelia miyamotoi in duplicate on an ABI 7500 Fast Real-Time PCR System using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... and the resulting solution was used to perform multiplex PCR using primer pairs specific for each endogenous plasmid of strain B31 [61] and the DreamTaq Green DNA Polymerase (Thermo Scientific). For genomic DNA extraction ...
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bioRxiv - Neuroscience 2022Quote: ... Low Density Lipoprotein (LDL) from human plasma complexed to pHrodo red (pHrodo-LDL) (Invitrogen; #L34356) was used to determine LDL uptake in APOE3 and APOE4 iMGLs ...
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bioRxiv - Immunology 2023Quote: ... BMDM cultures were treated with 250ug/uL BODIPY™ FL complex Low Density Lipoprotein (Invitrogen) for 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... (tail vein) injection of high-density lipoprotein nanoparticles labeled with fluorophore DiR-HDL (D-12731; Invitrogen) in 0.9% sterile saline at 1 μmol DiR/kg as described (25) ...
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bioRxiv - Immunology 2022Quote: ... Cell surface antibodies (ThermoFisher: CD3ε F506 ...
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bioRxiv - Immunology 2023Quote: ... delta-surface plates (Nunc) were pre-coated with 12 μg/ml polyclonal anti-hamster IgG (MP Biomedicals ...
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bioRxiv - Neuroscience 2024Quote: ... 50 μL (1 mg/ml) of fluorescent low-density lipoprotein (BODIPY™ FL LDL; L3483; Life Technologies) was injected through the retro-orbital vein under deep isoflurane anesthesia ...
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bioRxiv - Cell Biology 2023Quote: ... After 24 hours the medium was refreshed and oxidized lipoproteins (oxLDL, L34357, Thermo Fisher Scientific, 2.5 mg/ml) were added to the cultures ...
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bioRxiv - Biochemistry 2024Quote: ... Single cells were seeded onto 100 μg/mL poly-L-ornithine (Sigma-Aldrich)-coated surfaces in DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... burgdorferi strains grown under mammalian-like conditions were used for reverse transcription into cDNA using random primers (Thermo Fisher Scientific, Waltham, MA) and SuperScript III (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... burgdorferi strains grown under mammalian-like conditions were used for reverse transcription into cDNA using random primers (Thermo Fisher Scientific, Waltham, MA) and SuperScript III (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... cultured PACs were identified by the uptake of DiI-labeled acetylated low density lipoprotein (acLDL) (Invitrogen, Carlsbad, CA, USA) and simultaneous binding of FITC-labeled BS-1 lectin (BS-1 ...
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bioRxiv - Cell Biology 2020Quote: ... cultured PACs were identified by the uptake of DiI-labeled acetylated low density lipoprotein (acLDL) (Invitrogen, Carlsbad, CA, USA) and simultaneous binding of FITC-labeled BS-1 lectin (BS-1 ...
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bioRxiv - Biophysics 2022Quote: Protein concentrations in lipoprotein and EV samples were determined with Pierce(tm) BCA Protein Assay Kit (ThermoFisher, Rockford, USA) according to manufacturer instructions ...
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bioRxiv - Biophysics 2022Quote: Oocyte surface proteins were purified using the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific), modified here for oocytes as previously described (Lynagh ...
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bioRxiv - Synthetic Biology 2020Quote: ... Surface proteins were purified with the Pierce™ Cell Surface Protein Isolation Kit (Thermo Fisher Scientific). Purified surface proteins or total cell lysates were run on a 4–12 % BIS-TRIS gel (for P2X2 ...
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bioRxiv - Neuroscience 2020Quote: Cell surface proteins were purified using the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific) based on manufacturer instructions with a few modifications ...
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bioRxiv - Neuroscience 2021Quote: Fn14 KO and WT littermate mice and TWEAK KO and WT littermate mice at P27 were euthanized and their brains harvested in ice cold PBS (Gibco). Coronal sections of 300 μm were made on a VT1000S vibratome (Leica ...
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bioRxiv - Cell Biology 2021Quote: ... Sphera Low-Attachment Surface (Thermo Fisher) at a density of 4□×□104 cells/mL ...
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bioRxiv - Cell Biology 2020Quote: ... to label cell surface proteins (Invitrogen) (53) ...
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bioRxiv - Immunology 2022Quote: ... and surface proteins in PBS (Gibco) or 1X Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... surfaces wiped with RNaseZap (#AM9780, Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... Cell surface CD24 (mouse: Thermo Fisher 48-0242-82 ...
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bioRxiv - Immunology 2023Quote: ... Nunclon delta surface plates (Thermo Scientific) were coated with 100 µl of 5 µg/ml of M1 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were exposed to a low-oxygen condition at 0.1% O2 (Smartor 118, Hariolab, Guangzhou, China) or 50μg/ml oxidized low-density lipoprotein (oxLDL, Invitrogen, Eugene, OR) for 24 hours and 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... burgdorferi cells harvested and washed as described above were resuspended in PBS+Mg without or with proteinase K (Invitrogen, 200 µg/ml final concentration). Proteinase K-containing and control samples were incubated for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Cell surface proteins were biotinylated and isolated using Pierce Cell Surface Protein Isolation Kit (#89881, Thermo Fisher). All the steps were performed at 4°C unless otherwise mentioned ...
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bioRxiv - Systems Biology 2023Quote: EV surface protein enrichment was performed by Pierce cell surface protein biotinylation and isolation kit (Thermo Scientific) as previous study37 with a modification on washing procedure (replacing ultracentrifugation by ultrafiltration) ...
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bioRxiv - Cell Biology 2023Quote: ... a cell-surface biotinylation assay was performed using Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... The cell surface biotinylation assay was performed using the Pierce Cell Surface Biotinylation and Isolation kit (Thermo Fisher) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: Cell surface proteins were isolated by using Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific, cat#89881), following the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... on six-well Nunclon surface plates (Nunc) coated with Geltrex (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 12 Well Nunclon Delta Surface (Thermo Scientific), 96 Well Tissue Culture Plate (VWR 734-2327) ...
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bioRxiv - Neuroscience 2021Quote: ... Nunc Delta surface treated plates (Thermo Scientific) were coated as previously described (24) ...
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bioRxiv - Neuroscience 2021Quote: Fn14 KO and WT littermate mice and TWEAK KO and WT littermate mice at P27 were euthanized and their brains were bisected and flash frozen using liquid nitrogen in 1 mL of Trizol (Ambion; Naugatuck, CT) and kept at -80°C until processing ...