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Citations for Thermo Fisher :
1 - 45 of 45 citations for Blank SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 0.5 µl of SAP (Affymetrix), 0.1 µl E ...
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bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP, Affymetrix Cat # 78390) and Sanger sequenced.
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bioRxiv - Neuroscience 2022Quote: ... Then Shrimp Alkaline Phosphatase (SAP) reaction (total volume 10 µl) was performed with 10× SAP buffer (Affymetrix), 0.5 µl of SAP (Affymetrix) ...
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bioRxiv - Developmental Biology 2022Quote: ... purified by Exo-Sap-IT (Affymetrix, 78201) to remove unincorporated primer ...
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bioRxiv - Microbiology 2019Quote: ... DNA was treated with Exo-SAP-IT (ThermoFisher) and incubated at 37°C for 15 min followed by a 15 min incubation at 80°C to inactivate the enzyme ...
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bioRxiv - Genomics 2020Quote: ... with excess primers removed with EXO-SAP-IT (Affymetrix), and then amplified samples were diluted 1/5 in DNA suspension buffer ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR products were purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis (through Genewiz) ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis ...
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bioRxiv - Genomics 2019Quote: ... amplicons were cleaned with Exo-SAP (Affymetrix, Santa Clara, CA, USA), quantified with QuantiFluor dsDNA system (Promega ...
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bioRxiv - Genomics 2023Quote: ... 1 ul of EXO-SAP-IT Express (Life Technologies-75001.1.ML) was added to the reaction ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and processing blanks were evaporated using a Savant SpeedVac SPD210 (ThermoFisher). The resulting dried extracts were derivatized using methyoxyamine hydrochloride (MOC ...
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bioRxiv - Microbiology 2019Quote: ... 2 µl of EXO-SAP (100 U shrimp alkaline phosphatase (Thermo Scientific), 100 U Exonuclease I (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons were cleaned using Exo-SAP-IT (Affymetrix, Santa Clara, CA, USA), and Sanger-sequenced at the Core Genomic Facility at the University of Sheffield ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products were treated with Exo-Sap II (Thermo Fisher Scientific), and sequence termination reactions were performed from both directions of strands using BigDye Terminator v3.2 (Applied Biosystem) ...
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bioRxiv - Microbiology 2021Quote: ... Negative controls included extraction blanks and DNA-free water (Ambion Cat#: AM9935) as the template for the V4-16S rRNA PCR ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were treated with Exo-Sap (ExoSAP-IT™, Thermo Fisher, USA) to remove unutilized primers and dNTPs ...
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bioRxiv - Microbiology 2021Quote: Purified substrates and cleavage product mixtures were dephosphorylated by incubating with SAP (ThermoFisher, USA) at 37°C for 1 h according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2021Quote: ... we purified the amplified DNA using USB Exo-SAP-IT (Affymetrix, Santa Clara, CA) and bidirectionally sequenced the amplicons using an ABI 3130 DNA Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... the resulting DNA from the PCR reaction was purified via EXO-SAP cleanup (Affymetrix). Individual EXO-SAP reactions consisted of 1.55ul dH2O ...
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bioRxiv - Systems Biology 2022Quote: ... blank sample and carrier sample were labelled with TMTsixplex™ reagents (Thermo Fisher Scientific) in 40% (v/v ...
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bioRxiv - Plant Biology 2021Quote: Xylem sap protein concentrations were determined with Pierce® Coomassie Plus kit (Thermo Fisher Scientific), which is based on the Bradford method (Bradford ...
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bioRxiv - Microbiology 2021Quote: ... followed by purification using the Exo-SAP-IT PCR product clean-up reagent (Thermofisher, Australia) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Blank qPCR Master Mix kit (EURx, Poland) with specific TaqMan probes (Thermo Fisher Scientific, USA) were used (Table 1) ...
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bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with indicated plasmids or blank pMT-V5-His vector (Invitrogen, Cat# V412020) using the Effectene transfection reagent (Qiagen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... after enzymatic clean up with Exo-SAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) applying a 10x dilution of the Exo-SAP-IT but otherwise following the manufactureŕs instructions ...
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bioRxiv - Plant Biology 2021Quote: ... in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen; Carlsbad, CA, USA) for 1 hr at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... for single protein expression or (plasmids of interest – 0.2 µg + 0.2 µg, blank DNA – 0.6 µg) using lipofectamine 3000 (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... for single protein expression or (plasmids of interest – 0.2 µg + 0.2 µg, blank DNA – 0.6 µg) using lipofectamine 3000 (Thermo Fisher Scientific) or the Neon electroporator (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with a total of 1 µg DNA (0.3 µg + 0.3 µg plasmid of interest + 0.4 µg blank DNA) using lipofectamine 3000 (Thermo Fisher Scientific). The DNA sequence for the N-terminal mutant of EEA1 carrying F41A and I42A ...
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bioRxiv - Genomics 2023Quote: The double-stranded linkers were converted into single-stranded to successively allow the second strand synthesis by Shrimp Alkaline Phosphatase (1 U/μl SAP, catalog num. 78390, Affymetrix) and Uracil-Specific Excision Reagent (1 U/μl USER ...
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bioRxiv - Neuroscience 2020Quote: ... A spectral library was created in Skyline for some of the target peptides using several DDA of FMRpolyG samples as well as blanks spiked with AQUA Ultimate endogenous (light) and isotopically labeled (heavy) peptides obtained from ThermoFisher Scientific (Supplementary Table 1) ...
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bioRxiv - Microbiology 2023Quote: ... Extraction blanks were run with each round of DNA extractions and all returned no detectable nucleic acids using the maximum amount of blank sample (20μL) via the Qubit dsDNA High Sensitivity assay kit (ThermoFisher Scientific). For all 78 samples ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting cDNA underwent another final second round IVT step at 37 °C overnight and followed EXO-SAP treatment (Affymetrix 78200) for 15 minutes at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg BA disks were prepared by transferring 10 μL of preheated 500 mg/mL BA in Dimethyl sulfoxide (DMSO) stock to blank antimicrobial disks (6 mm, Fisher Scientific). Single disks containing either 5 mg BA or 25 μg FLC (6 mm ...
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bioRxiv - Microbiology 2020Quote: Ten milliliters of each water sample and Milli Q water (Blank) were filtered through a Nalgene™ 0.22 µm syringe filter (Thermo Scientific) to remove suspended particulates ...
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bioRxiv - Evolutionary Biology 2024Quote: ... BA disks were prepared by transferring 10 μL of preheated 500 mg/mL BA in dimethyl sulfoxide (DMSO) stock to blank antimicrobial disks (6 mm, Fisher Scientific). MH plates were incubated at 37 °C for 48 h ...
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bioRxiv - Genetics 2021Quote: ... The PCR products were purified with 3 μl Exo/SAP and then cycle-sequenced with the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to manufacturer instructions with BP15851 (M13F ...
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bioRxiv - Genomics 2020Quote: ... the aRNA was treated with 6 μL EXO-SAP (ExoSAP-IT™ PCR Product Cleanup Reagent, Thermo Fisher Scientific, Cat. # 78200.200.UL) at 37°C for 15 min followed by fragmentation with 5.5 μL fragmentation buffer (200 mM Tris-acetate (pH 8.1) ...
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bioRxiv - Plant Biology 2021Quote: ... Each sample was placed in a one cm plastic spectrophotometer cuvette and absorption spectra recorded from 480-800 nm versus a distilled water blank using a Genesis 10 UV Instrument (Thermo Fisher Scientific). The spectra were analyzed utilizing the Vision Lite software (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: ... Extracted nucleic acids from VLPs and two extraction blanks were used for cDNA synthesis using the SuperScript™ IV First-Strand Synthesis System (Invitrogen™) with random hexamers provided by the manufacturer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Posaconazole disks were prepared by adding 4 μL of 0.625 mg/mL posaconazole stock in DMSO to blank susceptibility disks (Fisher Scientific, Ottawa, ON, Canada) for a final concentration of 2.5 mg ...
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bioRxiv - Microbiology 2020Quote: ... blanks and internal standards were analyzed by gas chromatography (GC) coupled to an APPI-Q-Exactive HF mass spectrometer (Thermo Fisher Scientific, USA). GC was equipped with a DB-5 column (30 m x 0.25 mm i.d. ...
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bioRxiv - Plant Biology 2021Quote: 5 μg of RNA from 5-day-old seedlings was first treated with 1 unit of Shrimp Alkaline Phosphatase (SAP; USB Products, Affymetrix, Inc.; Cleveland, OH, USA) in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... One millilitre of fresh sap or control sample was freeze-dried using a SpeedVac concentrator unit (model Savant SPD121P, Thermo Scientific, Waltham, MA, USA) at 40 □ ...
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bioRxiv - Molecular Biology 2024Quote: ... a cuvette with 1.0 mL of sterile NB was used as a blank for measuring optical density at 600 nm (OD600) using the GENESYS™ 10S UV-Vis Spectrophotometer (Thermo Fisher Scientific, MA). Samples of the subculture were measured at 0 ...