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Citations for Thermo Fisher :
1 - 50 of 1451 citations for Beta glucuronidase GUSB E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... GUSB (Applied Biosystems), using Promyelocytic Leukemia (HL-60 ...
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bioRxiv - Cell Biology 2019Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
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bioRxiv - Genetics 2019Quote: pET22b-mZCWPW1-His construct was transformed into BL21 (DE3) E.Coli cells (Invitrogen). Bacterial cultures were grown to a density with O.D600∼0.7 ...
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bioRxiv - Cell Biology 2019Quote: ... The plasmids encoding for His-SEPT2 or His-SEPT2-mCherry and SEPT6/7-strep were co-transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 2-3 and induced with 1 mM IPTG for 1h at 37°C (His-SEPT2 ...
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bioRxiv - Neuroscience 2022Quote: ... The GUSB probe/primer (#Hs009627_m1, Thermo Fisher Scientific) was used as a normalizer for gene expression analysis.
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bioRxiv - Cancer Biology 2021Quote: ... SLC6A8 and GUSB (4331182, Fisher Scientific, Waltham, MA) were used to perform the reactions in a Step One plus Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... and GusB (TaqMan Gene expression assay from ThermoFisher, Hs99999908_m1).
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bioRxiv - Biochemistry 2022Quote: ... METTL7B and GUSB were obtained from Fisher Scientific (Hampton, NH).
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bioRxiv - Cell Biology 2019Quote: ... Stbl3 E.coli (Invitrogen) was used for all pCMV AAV vector cloning.The in-frame stop codon in the BGH 3’ UTR was removed to allow for readthrough into the poly(A ...
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bioRxiv - Biochemistry 2020Quote: ... with a N-terminal 8x His tag was expressed from the pSJ2 vector (84) in BL21 E.coli and purified by Ni-NTA resin (Thermo Fisher). Full-length human Src kinase (WT or K298M ...
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bioRxiv - Microbiology 2021Quote: ... E.coli strain DH5α (Invitrogen) was first transformed with the pSIM5 plasmid (pSC101 repAts ...
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bioRxiv - Molecular Biology 2023Quote: ... E.coli DH10B (Thermo Scientific) or E.coli XL10-Gold (Agilent Technologies ...
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bioRxiv - Biophysics 2024Quote: ... CD44 (Hs00153304_m1) and the housekeeping genes GAPDH (Hs99999905_m1) and GusB (Hs99999908_m1) were from Thermofisher. Real time PCR were carried out on the 7500 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... Subsequently the β-glucuronidase reporter gene was recombined using pENTR-GUS (Invitrogen). For translational fusions of MpBELL2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... BL21-AI E.coli (Thermo Fisher) were transformed with mCherry-pBAD (gift from Michael Davidson & Nathan Shaner & Roger Tsien ...
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bioRxiv - Genomics 2019Quote: ... instrument with TaqMan Gene Expression Assays (Hs01902432_s1 for RAC1 and Hs99999908_m1 for GUSB; Thermo Fisher Scientific). The relative gene expression levels of RAC1 were calculated by the 2−ΔΔCT method using GUSB as the endogenous control for normalization ...
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bioRxiv - Genetics 2020Quote: ... MSH6 and GUSB (Hs99999908_m1) or 1.8 µM primer mix (IDT) and 250 nM probes (ThermoFisher Scientific) for GAPDH and UBC ...
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bioRxiv - Biophysics 2019Quote: E.coli strain BL21 (DE3) (ThermoFisher Scientific) was used for CBMCipA production ...
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bioRxiv - Microbiology 2020Quote: ... Relative amount was normalized over the expression of housekeeping gene GUSB (Hs99999908_m1, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2019Quote: ... E.coli BL21 Star (DE3) cells (Life Technologies) transformed with pPR-IBA2-CLASP2 (1213-1515 ...
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bioRxiv - Genomics 2019Quote: ... Plasmids were transformed into GT115 E.coli (Invitrogen) and DNA isolated using PureYield Plasmid Midiprep system (Promega) ...
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bioRxiv - Biophysics 2020Quote: ... and expressed in E.coli BL21 (DE3) (Invitrogen). The wt-p53 (1-321 aa ...
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bioRxiv - Genetics 2020Quote: ... ccdB resistant chemically competent E.coli (Invitrogen, A10460) were transformed with plasmids containing a Gateway cassette (ccdB ...
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bioRxiv - Neuroscience 2019Quote: ... microglia were incubated with 50 µL of E.coli particle suspension (pHrodo™ Green E.coli BioParticles™, P35366, Invitrogen™) for 60 min ...
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bioRxiv - Systems Biology 2021Quote: ... beta-mercaptoethanol (Gibco), sodium pyruvate (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... beta-mercaptoethanol (Gibco), penicillin ...
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bioRxiv - Systems Biology 2021Quote: ... beta-mercaptoethanol (Gibco) and recombinant mouse leukemia inhibitory factor (mLIF ...
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bioRxiv - Molecular Biology 2020Quote: ... Beta mercaptoethanol (Gibco), glucose (Kemika) ...
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bioRxiv - Cell Biology 2022Quote: ... beta-mercaptoethanol (Gibco) 1:1000 ...
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bioRxiv - Immunology 2021Quote: ... 100uL of 1mg/mLpHrodo Red E.coli BioParticles (Invitrogen) were added in PBS and cells placed at 4°C (negative control ...
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bioRxiv - Genetics 2019Quote: ... and transformed into E.coli DH5α competent cells (Invitrogen) for plasmid purification and validation through Sanger sequencing.
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bioRxiv - Genomics 2022Quote: ... 0.14 U E.coli DNA Ligase (Invitrogen, 18052-019), 0.56 U E.coli DNA Polymerase I (Invitrogen ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... pHrodo® Red E.coli Bioparticles® (Life Technologies) were resuspended in 2 ml of Live Cell Imaging Solution (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... transformed into E.coli BL21(DE3) (Thermo Fisher Scientific) and purified as described before (Ganz et al. ...
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bioRxiv - Plant Biology 2021Quote: ... which contained a β-glucuronidase (GUS) gene fusion created via LR Clonase (Thermo Fisher Scientific, Waltham, MA, USA) reactions.
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bioRxiv - Molecular Biology 2022Quote: ... GusB was used as the endogenous control (expression was unaffected by experimental conditions) according to the Taqman PCR protocol (Applied Biosystems). All primers and probes were 90 – 100% efficient as examined by standard curve analysis (data not shown) ...
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bioRxiv - Cell Biology 2023Quote: ... The relative expression of target genes was quantified using the comparative threshold cycle (Ct) method and normalized to the TaqMan GUSB transcript (Applied Biosystems) as the endogenous housekeeping gene ...
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bioRxiv - Genetics 2021Quote: ... of One Shot TOP10 Chemically Competent E.Coli (ThermoFisher #C404003) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Delivery of pHrodo Red E.coli BioParticles Conjugate (Molecular Probes) was performed with a similar procedure at - 2.5 mm A-P ...
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bioRxiv - Genomics 2022Quote: ... 0.56 U E.coli DNA Polymerase I (Invitrogen, 18010-025), 0.03 U RNase H (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... This was subsequently transformed into ccdB Survival E.coli (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... pHrodo™ Green E.coli Bioparticles (ThermoFisher, P35366; 3μg/ml), pHrodo™ Red LDL (ThermoFisher ...
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bioRxiv - Synthetic Biology 2020Quote: Proteins were expressed from E.coli BL21-AI strains (Invitrogen) carrying appropriate plasmids for expression of E.coli Gnd or E.coli RpiA (ribose-5-phosphate isomerase ...
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bioRxiv - Cell Biology 2023Quote: ... with Top10 E.coli competent cells (#K450001SC, ThermoFisher, Waltham, MA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Plasmids were amplified using E.Coli DH5α strain (Life Technologies). DNA was then purified using the NucleoBond® Xtra Plus Midi-Prep kit (Machery-Nagel®) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ligated plasmids were transformed into DH5α E.coli (Life Technologies). The PX330-Alk-Eml4 plasmid was kindly provided by Andrea Ventura [26].
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bioRxiv - Microbiology 2019Quote: ... beta-Actin pAb (Invitrogen), MNV NS1/2 (a gift from Dr ...
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bioRxiv - Microbiology 2020Quote: ... 0.1% beta-mercaptoethanol (Gibco) for 6 days at 37 °C and 9.5% CO2 ...
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bioRxiv - Cancer Biology 2019Quote: ... 50μM beta-mercaptoethanol (Gibco), 1X Cell Stimulation Cocktail (eBioscience) ...
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bioRxiv - Immunology 2021Quote: ... 50μM beta-mercapthoethanol (Gibco)) ...