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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Anti NET SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 0.5 µl of SAP (Affymetrix), 0.1 µl E ...
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bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP, Affymetrix Cat # 78390) and Sanger sequenced.
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bioRxiv - Neuroscience 2022Quote: ... Then Shrimp Alkaline Phosphatase (SAP) reaction (total volume 10 µl) was performed with 10× SAP buffer (Affymetrix), 0.5 µl of SAP (Affymetrix) ...
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bioRxiv - Developmental Biology 2022Quote: ... purified by Exo-Sap-IT (Affymetrix, 78201) to remove unincorporated primer ...
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bioRxiv - Microbiology 2019Quote: ... DNA was treated with Exo-SAP-IT (ThermoFisher) and incubated at 37°C for 15 min followed by a 15 min incubation at 80°C to inactivate the enzyme ...
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bioRxiv - Genomics 2020Quote: ... with excess primers removed with EXO-SAP-IT (Affymetrix), and then amplified samples were diluted 1/5 in DNA suspension buffer ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR products were purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis (through Genewiz) ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis ...
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bioRxiv - Genomics 2019Quote: ... amplicons were cleaned with Exo-SAP (Affymetrix, Santa Clara, CA, USA), quantified with QuantiFluor dsDNA system (Promega ...
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bioRxiv - Genomics 2023Quote: ... 1 ul of EXO-SAP-IT Express (Life Technologies-75001.1.ML) was added to the reaction ...
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bioRxiv - Microbiology 2023Quote: ... hair nets and TyVek sterile sleeves (Cat.No.17988110, Fisher Scientific, USA) and the use of 10% bleach spray to treat surfaces including gloves and sleaves ...
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bioRxiv - Microbiology 2019Quote: ... 2 µl of EXO-SAP (100 U shrimp alkaline phosphatase (Thermo Scientific), 100 U Exonuclease I (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons were cleaned using Exo-SAP-IT (Affymetrix, Santa Clara, CA, USA), and Sanger-sequenced at the Core Genomic Facility at the University of Sheffield ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products were treated with Exo-Sap II (Thermo Fisher Scientific), and sequence termination reactions were performed from both directions of strands using BigDye Terminator v3.2 (Applied Biosystem) ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was diluted with 400 μl of NET-2 buffer and antibody-conjugated Dynabeads M-280 Sheep anti-mouse IgG (ThermoFisher Scientific) beads were added ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were treated with Exo-Sap (ExoSAP-IT™, Thermo Fisher, USA) to remove unutilized primers and dNTPs ...
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bioRxiv - Microbiology 2021Quote: Purified substrates and cleavage product mixtures were dephosphorylated by incubating with SAP (ThermoFisher, USA) at 37°C for 1 h according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2021Quote: ... we purified the amplified DNA using USB Exo-SAP-IT (Affymetrix, Santa Clara, CA) and bidirectionally sequenced the amplicons using an ABI 3130 DNA Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... the resulting DNA from the PCR reaction was purified via EXO-SAP cleanup (Affymetrix). Individual EXO-SAP reactions consisted of 1.55ul dH2O ...
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bioRxiv - Immunology 2020Quote: NETs was quantified using the Quanti-iT™ PicoGreen® dsDNA kit (Invitrogen, UK). Supernatants were also tested using a capture ELISA ...
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bioRxiv - Immunology 2020Quote: ... NETs were stained by directly adding SYTOX™ Green and 2μM Hoechst 33342 (ThermoFisher) for 30min at room temperature to the wells ...
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bioRxiv - Immunology 2022Quote: ... to detect NETs and cell-permeable DNA dye Sytox Green (250 nM, Thermo Fisher) to determine the total number of cells ...
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bioRxiv - Systems Biology 2022Quote: ... Net absorbances were plotted against BSA protein concentration for standard curve generation (ThermoFisher, 23208). For each sample ...
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bioRxiv - Plant Biology 2021Quote: Xylem sap protein concentrations were determined with Pierce® Coomassie Plus kit (Thermo Fisher Scientific), which is based on the Bradford method (Bradford ...
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bioRxiv - Microbiology 2021Quote: ... followed by purification using the Exo-SAP-IT PCR product clean-up reagent (Thermofisher, Australia) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... MPO-bound DNA (NETs) was quantified using the Quant-iT PicoGreen kit (Invitrogen; cat. P11496) as previously described (Veras et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... The Average Net Mean Fluorescence Intensity was read on a Luminex FLEXMAP 3D System (Invitrogen). Signals from RPGs were normalized internally to those from GAPDH ...
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bioRxiv - Immunology 2019Quote: ... DNA concentration in the supernatants (referred to as NETs) was determined using Picogreen dsDNA kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... Neutrophils and NETs were visualized under a fluorescence microscope (EVOS cell imaging system, Thermo Fisher Scientific).
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bioRxiv - Evolutionary Biology 2023Quote: ... after enzymatic clean up with Exo-SAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) applying a 10x dilution of the Exo-SAP-IT but otherwise following the manufactureŕs instructions ...
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bioRxiv - Biochemistry 2023Quote: ... NET DNA concentration was determined by Quant-iT Picogreen dsDNA reagent as per manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen; Carlsbad, CA, USA) for 1 hr at 37°C ...
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bioRxiv - Immunology 2021Quote: ... The DNA content of NETs and chromatin was assessed by PicoGreen assay according to the manufacturer’s instructions (Thermofisher Scientific). Beginning at 1 µg/ml (of DNA content) ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were resuspended in 400 ul NET-2 and extracted with an equal volume of acid-phenol:chloroform (Invitrogen AM9722). After centrifugation at 16,000 x g for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were resuspended in 400 μl NET-2 and extracted with an equal volume of phenol:chloroform:isoamyl 25:24:1 (Invitrogen 15593031). After centrifugation at 16,000 x g for 15 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... The final concentration of NETs in terms of both NETDNA and entrapped proteins was measured on a NanoDrop2000 (Thermofisher) using the nucleic acid 260/280 function and protein 280 and 205 functions ...
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bioRxiv - Plant Biology 2024Quote: SAMs dissected in the net house were immediately transferred to 300 μl RNAlater™ solution (Thermo Fisher Scientific, USA) for storage at -18°C ...
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bioRxiv - Genomics 2023Quote: The double-stranded linkers were converted into single-stranded to successively allow the second strand synthesis by Shrimp Alkaline Phosphatase (1 U/μl SAP, catalog num. 78390, Affymetrix) and Uracil-Specific Excision Reagent (1 U/μl USER ...
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bioRxiv - Bioengineering 2022Quote: ... The mixed cell suspension was dispensed into a large dimple plate (Tissue by Net Ltd., Tokyo, Japan) for mixed spheroids formation in Essential 8 Medium (A1517001 Invitrogen) for four days before being collected and transferred to a 3D printed mold ...
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bioRxiv - Immunology 2022Quote: ... NETs/NLS were imaged at 4 h post stimulation using 20X magnification on a EVOS FL Auto Microscope (Thermo Scientific).
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bioRxiv - Bioengineering 2023Quote: ... The remaining structures were resuspended with buffer (0.5xTBE with net 10mM MgCl2) and quantified by NanoDrop (NanoDrop 2000C Spectrophotometer, Thermo Scientific). The structures were diluted to 1 nM for downstream imaging.
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bioRxiv - Cancer Biology 2024Quote: ... NETs were quantified by collecting the supernatant and measuring cellular free DNA using Quant-iT Picogreen (Invitrogen, Grand Island, NY) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting cDNA underwent another final second round IVT step at 37 °C overnight and followed EXO-SAP treatment (Affymetrix 78200) for 15 minutes at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... The PCR products were purified with 3 μl Exo/SAP and then cycle-sequenced with the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to manufacturer instructions with BP15851 (M13F ...
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bioRxiv - Genomics 2020Quote: ... the aRNA was treated with 6 μL EXO-SAP (ExoSAP-IT™ PCR Product Cleanup Reagent, Thermo Fisher Scientific, Cat. # 78200.200.UL) at 37°C for 15 min followed by fragmentation with 5.5 μL fragmentation buffer (200 mM Tris-acetate (pH 8.1) ...
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bioRxiv - Immunology 2019Quote: ... NETs were quantified in the culture supernatant via the Quant-iT™ PicoGreen® dsDNA Assay kit (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: 5 μg of RNA from 5-day-old seedlings was first treated with 1 unit of Shrimp Alkaline Phosphatase (SAP; USB Products, Affymetrix, Inc.; Cleveland, OH, USA) in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... One millilitre of fresh sap or control sample was freeze-dried using a SpeedVac concentrator unit (model Savant SPD121P, Thermo Scientific, Waltham, MA, USA) at 40 □ ...
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bioRxiv - Neuroscience 2019Quote: ... anti-anti (Gibco) and B27 (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-Anti (Gibco/Thermo Fisher Scientific ...