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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Anti ChAT SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2023Quote: ... anti-CHAT goat (Invitrogen), Alexa Fluor 488 donkey anti-rabbit ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 µl of SAP (Affymetrix), 0.1 µl E ...
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bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP, Affymetrix Cat # 78390) and Sanger sequenced.
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bioRxiv - Neuroscience 2022Quote: ... Then Shrimp Alkaline Phosphatase (SAP) reaction (total volume 10 µl) was performed with 10× SAP buffer (Affymetrix), 0.5 µl of SAP (Affymetrix) ...
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bioRxiv - Neuroscience 2024Quote: ... mouse monoclonal anti-ChAT (1:250, Thermo Fisher, MA5-31383), rabbit polyclonal anti-PV (1:250 ...
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bioRxiv - Developmental Biology 2022Quote: ... purified by Exo-Sap-IT (Affymetrix, 78201) to remove unincorporated primer ...
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bioRxiv - Cell Biology 2021Quote: ... ChAT (703789, ThermoFisher Scientific), VAChT (Cat# 24286 ...
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bioRxiv - Genetics 2023Quote: ... mouse anti-ChAT (DSHB; 1:50) rabbit anti-GFP (Invitrogen A6455; 1:3000), rabbit anti-DsRED (Clontech #632496 ...
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bioRxiv - Microbiology 2019Quote: ... DNA was treated with Exo-SAP-IT (ThermoFisher) and incubated at 37°C for 15 min followed by a 15 min incubation at 80°C to inactivate the enzyme ...
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bioRxiv - Plant Biology 2021Quote: Xylem sap protein concentrations were determined with Pierce® Coomassie Plus kit (Thermo Fisher Scientific), which is based on the Bradford method (Bradford ...
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bioRxiv - Genomics 2020Quote: ... with excess primers removed with EXO-SAP-IT (Affymetrix), and then amplified samples were diluted 1/5 in DNA suspension buffer ...
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bioRxiv - Neuroscience 2020Quote: ... for ChAT-Cre/Ai9 retinas or with rabbit anti-GFP (1:1000, Invitrogen) for ChAT-Cre/Ai95 retinas ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR products were purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis (through Genewiz) ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis ...
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bioRxiv - Genomics 2019Quote: ... amplicons were cleaned with Exo-SAP (Affymetrix, Santa Clara, CA, USA), quantified with QuantiFluor dsDNA system (Promega ...
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bioRxiv - Genomics 2023Quote: ... 1 ul of EXO-SAP-IT Express (Life Technologies-75001.1.ML) was added to the reaction ...
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bioRxiv - Microbiology 2019Quote: ... 2 µl of EXO-SAP (100 U shrimp alkaline phosphatase (Thermo Scientific), 100 U Exonuclease I (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons were cleaned using Exo-SAP-IT (Affymetrix, Santa Clara, CA, USA), and Sanger-sequenced at the Core Genomic Facility at the University of Sheffield ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products were treated with Exo-Sap II (Thermo Fisher Scientific), and sequence termination reactions were performed from both directions of strands using BigDye Terminator v3.2 (Applied Biosystem) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then transferred to fresh 0.3% PBS-Triton and incubated overnight at 4°C with appropriate primary antibodies (1:200-500 dilution of goat anti-ChAT IgG, Millipore, AB114P; 1:500 rabbit anti-GFP IgG, Abcam, ab6556 or 1:300 rabbit anti-GFP IgG, ThermoFisher, A-6455 (both anti-GFP had similar level of expression)).
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bioRxiv - Genetics 2021Quote: ... The PCR products were purified with 3 μl Exo/SAP and then cycle-sequenced with the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to manufacturer instructions with BP15851 (M13F ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were treated with Exo-Sap (ExoSAP-IT™, Thermo Fisher, USA) to remove unutilized primers and dNTPs ...
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bioRxiv - Microbiology 2021Quote: Purified substrates and cleavage product mixtures were dephosphorylated by incubating with SAP (ThermoFisher, USA) at 37°C for 1 h according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2021Quote: ... we purified the amplified DNA using USB Exo-SAP-IT (Affymetrix, Santa Clara, CA) and bidirectionally sequenced the amplicons using an ABI 3130 DNA Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... the resulting DNA from the PCR reaction was purified via EXO-SAP cleanup (Affymetrix). Individual EXO-SAP reactions consisted of 1.55ul dH2O ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and incubated overnight at 4°C with anti-ChAT (choline acetyltransferase) goat polyclonal antibody (1:500, Invitrogen #PIPA518518) and anti-c-Fos rabbit polyclonal antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... and α-choline acetyltransferase (ChAT) (Thermofisher: PA1-9027, 1:100).
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bioRxiv - Neuroscience 2023Quote: ... (Ross et al., 2010), goat anti-ChAT (1:200, Millipore AB144P, RRID:AB_2079751, rabbit anti-Lucifer Yellow (1:2000, ThermoFisher Scientific A-5750, RRID:AB_2536190), mouse anti-calretinin (1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... followed by purification using the Exo-SAP-IT PCR product clean-up reagent (Thermofisher, Australia) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Predesigned primers and probes for ChAT were purchased from Life Technologies. The mRNA for these targets was measured by RT-qPCR using ABI QuantStudio 7 flex Sequence detector ...
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bioRxiv - Neuroscience 2020Quote: ... every 10th section was processed immunohistochemically for ChAT using the same antibody as above and a donkey anti-goat Alexa 594 secondary antibody (Invitrogen/Thermofisher catalogue numbers ...
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bioRxiv - Evolutionary Biology 2023Quote: ... after enzymatic clean up with Exo-SAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) applying a 10x dilution of the Exo-SAP-IT but otherwise following the manufactureŕs instructions ...
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bioRxiv - Plant Biology 2021Quote: ... in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen; Carlsbad, CA, USA) for 1 hr at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... every 10th section was processed immunohistochemically for ChAT using the same antibody as above and a donkey anti-goat Alexa 594 secondary antibody (Invitrogen/Thermofisher catalogue numbers: A-11058). Motoneurone counts were made manually using an epifluorescent microscope (Axioplan 2 ...
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bioRxiv - Neuroscience 2023Quote: ... we incubated the tissue with the primary antibodies against ChAT and PV described above with a blue secondary antibody (donkey anti-rabbit AF350, Cat#A10039, Thermo Fisher Scientific; 1:200) or (donkey anti-sheep AF350 ...
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bioRxiv - Genomics 2023Quote: The double-stranded linkers were converted into single-stranded to successively allow the second strand synthesis by Shrimp Alkaline Phosphatase (1 U/μl SAP, catalog num. 78390, Affymetrix) and Uracil-Specific Excision Reagent (1 U/μl USER ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting cDNA underwent another final second round IVT step at 37 °C overnight and followed EXO-SAP treatment (Affymetrix 78200) for 15 minutes at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... the aRNA was treated with 6 μL EXO-SAP (ExoSAP-IT™ PCR Product Cleanup Reagent, Thermo Fisher Scientific, Cat. # 78200.200.UL) at 37°C for 15 min followed by fragmentation with 5.5 μL fragmentation buffer (200 mM Tris-acetate (pH 8.1) ...
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bioRxiv - Plant Biology 2021Quote: 5 μg of RNA from 5-day-old seedlings was first treated with 1 unit of Shrimp Alkaline Phosphatase (SAP; USB Products, Affymetrix, Inc.; Cleveland, OH, USA) in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... One millilitre of fresh sap or control sample was freeze-dried using a SpeedVac concentrator unit (model Savant SPD121P, Thermo Scientific, Waltham, MA, USA) at 40 □ ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-polyvalent HRP-Streptavidin Kit (Thermo Scientific) with DAB was used for the visualization of antigenic sites.
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
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bioRxiv - Immunology 2022Quote: ... PTBPs anti-bodies were conjugated to different fluorophores with anti-body labeling kits from ThermoFisher (AF488 ...
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bioRxiv - Developmental Biology 2022Quote: ... the AbC anti-rat/hamster compensation bead kit (Life Technologies) was used ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5 μl of APC-conjugated anti-c-KIT (Invitrogen, CD11705) antibodies for 20 minutes at room temperature with rotation at 10 revolutions per minutes (rpm ...
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bioRxiv - Cell Biology 2023Quote: ... the anti-rat/hamster compensation bead kit and the ArC amine reactive compensation bead kit (Life Technologies, A10628) was used ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoblotting was performed using the Western Breeze Chemiluminescence Anti-Rabbit kit (Invitrogen) and anti-GFP polyclonal antibody (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... and human Treg expansion kit anti-CD3/CD28 beads (Miltenyi Biotec/Invitrogen), using x-vivo 15 cell medium (Lonza ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoblotting was performed using the Western Breeze Chemiluminescence Anti-Rabbit kit (Invitrogen) and anti-GFP polyclonal antibody (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... The anti-ST6GAL1 and anti-FUT8 Abs were labeled with Alexa Fluor 488 labeling kit (Life Technologies GmbH; #A20181). 20 million cells were recorded per sample ...