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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Adenovirus Type 5 Particles CMV Luciferase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... entry vectors were recombined with the adenovirus type 5 destination vector pAd5-CMV-V5-DEST using LR Clonase (Invitrogen). pAd5 vectors carrying candidate gene vectors were further sequenced to confirm gene identity ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... adenovirus vectors were recombined with pAd/CMV/V5-DEST vectors (Life Technologies). Recombinant adenoviruses were produced in 293A cells (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen), as per manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: Generation of replication-defective recombinant adenovirus expressing N-SREBPs under CMV promoter was performed using ViraPower Adenoviral Expression System (#K4930-00, Invitrogen) as described earlier (54) ...
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bioRxiv - Cell Biology 2020Quote: ... Production and expression of adenovirus encoding mCherry/mCherry-KIF4A was performed using pAd/CMV/V5-DEST Gateway Vector Kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Anti-adenovirus A5 antibodies (ThermoFisher), Anti-influenza A virus NP antibodies (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... the promoter reporter vector and the pRL-CMV Renilla luciferase plasmid using Lipofectamine 2000 reagent (Invitrogen, USA). After transfection for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were loaded onto a micro precolumn (300 μm i.d. × 5 mm, particles were C18 PepMap 100, 5 μm particle size, 100 Å pore size, Thermo Fisher Scientific) using the loading pump for 3 min ...
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bioRxiv - Biochemistry 2020Quote: ... containing 5 µm PGC particles (Thermo Scientific, Waltham, MA, USA) connected to an LTQ-ion trap mass spectrometer (Thermo Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... ×5 mm C18 PepMap 100 trap column with 5 μm particle size (Thermo Fisher Scientific) to desalt and concentrate the samples before loading onto the C18 nanocolumn for separation ...
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bioRxiv - Biochemistry 2019Quote: ... ×5 mm C18 PepMap 100 trap column with 5 μm particle size (Thermo Fisher Scientific) to desalt and concentrate the samples before loading onto the C18 nanocolumn for separation ...
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bioRxiv - Genomics 2022Quote: ... reaction delivery into pAd-V5 Gateway Adenovirus destination vector (Thermofisher). Viral production and amplification in HEK293 cells as previously described (Golan-Lagziel et al. ...
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bioRxiv - Genomics 2022Quote: ... reaction delivery into pAd-V5 Gateway Adenovirus destination vector (Thermofisher). Viral production and amplification in HEK293 cells as previously described (Golan-Lagziel et al. ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 × 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to a Linear Trap Quadrupole Orbitrap Velos (with CID ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 x 0.075 mm, 2 μm particle size (Thermo Scientific), coupled to a Q Exactive HF-X Orbitrap (with HCD ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µm particle size) and an analytical column (PepMap C18, 500 x 0.075 mm, 2 µm particle size (Thermo Scientific), coupled to a Q Exactive HF-X Orbitrap (with HCD ...
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bioRxiv - Microbiology 2020Quote: ... The two amplicons were assembled with a 5’ CMV promoter amplified from pcDNA3.1 (Invitrogen) by Gibson assembly (New England Biosciences (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplicons were assembled with a 5′ cytomegalovirus (CMV) promoter amplified from pcDNA3.1 (Invitrogen) by Gibson assembly (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 x 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to a Orbitrap Eclipse mass spectrometer (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 x 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to an Orbitrap Eclipse mass spectrometer (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... Wild type and catalytic mutant H672N RNase L plasmids cDNAs (the backbone is pcDNA5-FRT-TO with CMV promoter, Invitrogen) used in this study were a generous gift from Dr ...
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bioRxiv - Cancer Biology 2023Quote: Adenovirus vectors were generated using the Virapower Adenoviral expression system (Invitrogen). Open reading frames of Osm and Stat3-Y705F following a CACC sequence were cloned into a pENTR-D-TOPO plasmid and then recombined into the pAd-CMV-DEST adenoviral plasmid using LR clonase II ...
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bioRxiv - Cancer Biology 2023Quote: Adenovirus vectors were generated using the Virapower Adenoviral expression system (Invitrogen). Briefly ...
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bioRxiv - Physiology 2022Quote: ... The ventricles were dissected into 3-mm2 columns and shaken at 800 rpm for 90–120 min at 30°C with 750 µL digestion buffer (12.5 µM CaCl2, 5 mg/mL collagenase type II [Thermo Fisher Scientific Inc., Waltham, MA, USA], 5 mg/mL collagenase type IV [Thermo Fisher Scientific Inc.] ...
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bioRxiv - Cancer Biology 2023Quote: ... using 5 mg/mL collagenase type II (ThermoFisher, 17101015) in DMEM/F12+ medium - supplemented with Glutamax ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were loaded onto the 2-cm Nano Trap column with an inner diameter of 100 µm packed with C18 particles of 5 µm particle size (Thermo Fisher Scientific) and were separated by reversed-phase chromatography using a 25-cm column with an inner diameter of 75 µm ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were loaded onto the 2-cm Nano Trap column with an inner diameter of 100 μm packed with C18 particles of 5 μm particle size (Thermo Fisher Scientific) and were separated by reversed-phase chromatography using a 25-cm column with an inner diameter of 75 μm ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were loaded onto the 2-cm Nano Trap column with an inner diameter of 100 μm packed with C18 particles of 5 μm particle size (Thermo Fisher Scientific) and were separated by reversed-phase chromatography using a 25-cm column with an inner diameter of 75 μm ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were loaded onto the 2-cm Nano Trap column with an inner diameter of 100 μm packed with C18 particles of 5 μm particle size (Thermo Fisher Scientific) and were separated by reversed-phase chromatography using a 25-cm column with an inner diameter of 75 μm ...
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bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 0.06 μg of CMV-promoter driven plasmid encoding SARS-CoV-2 wild type or mutant spike variants using Lipofectamine™ 2000 transfection reagent (ThermoFisher). Pseudovirus was harvested by collecting supernatant 48 h after transfection and filter sterilized (0.44 μm ...
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bioRxiv - Immunology 2020Quote: ... Biobasic SEC-300 (300 x 7.8 mm, particle size; 5 µm, ThermoFisher, USA) column was used with 1.0 mL/minute flow rate ...
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bioRxiv - Pathology 2022Quote: ... under the control of a cytomegalovirus promoter (ViraPower Adenovirus Expression System, Invitrogen). The AAV-Tau was verified by sequencing and the production of Tau by western blot ...
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bioRxiv - Cancer Biology 2020Quote: ... Twenty-five µL of adenovirus was mixed with 600 µL DMEM (Gibco) and 3 µL 2M CaCl2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty-five microliters of adenovirus were mixed with 600 µL DMEM (Gibco) and 3 µL 2 M CaCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... adenovirus transduced neuronal cells from a six-well plate (Nunc Thermo Scientific) were collected in cold PBS and centrifuged at 750g at 4 °C for 5 min to collect the neuronal cell pellet ...
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bioRxiv - Neuroscience 2023Quote: ... adenovirus transduced neuronal cells from a six-well plate (Nunc Thermo Scientific) were collected in cold PBS and centrifuged at 750g at 4 °C for 5 min to collect the neuronal cell pellet ...
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The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... Peptides were loaded onto the 2-cm Nano Trap column with an inner diameter of 100 μm packed with C18 particles of 5 μm particle size (Thermo Fisher Scientific, WA, USA) and were separated by reversed-phase chromatography using a 25-cm column with an inner diameter of 75 μm ...
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bioRxiv - Cancer Biology 2023Quote: ... pAd/CMV/DEST (Thermofisher Scientific), using the Gateway cloning system.
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bioRxiv - Immunology 2024Quote: ... Luciferase-labeled cell lines were generated through infection with pTomo-CMV-luciferase-IRES-puro lentivirus (MOI≈10) and subsequently selected with puromycin (Life Technologies, 1 µg/ml) for 2 weeks ...
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bioRxiv - Cancer Biology 2019Quote: ... the wild-type and mutant 3’UTRs were cloned into a luciferase reporter vector (pmiR-REPORT, Ambion, USA) at the SpeI and HindIII site to generate Luc-HIF1α-WT and Luc-HIF1α-Mut constructs ...
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bioRxiv - Cell Biology 2023Quote: ... or pLenti-CMV-Dendra2×3-P2A-H2B-miRFP after a 5 minute incubation in OptiMEM (Gibco, #31985070) with 40 µg linear PEI (polsciences ...
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bioRxiv - Cancer Biology 2021Quote: ... with 5% FBS and 0.5 mg/ml collagenase type I (Gibco) and placed in a shaking incubator at 50rpm and 37°C for 75min ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions were loaded onto a cartridge precolumn (5 mm x ID 300 μm, C18 PepMap 100 A, 5 μm particles (ThermoFisher)) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were loaded onto the trap column (C18 PepMap100, 5 μm particle size, 300 μm x 5 mm, Thermo Scientific) for 4 min at 18 μl/min ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample was concentrated over an Acclaim PepMap C18 pre-column (5 μm particle size, 0.3 mm ID x 5 mm length, ThermoFisher Scientific) then bound to a 50 cm EasySpray C18 analytical column (2 μm particle size ...
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bioRxiv - Cell Biology 2023Quote: ... An Acclaim PepMap C18 trap-column (5 µm particle size, 0.3 mm ID x 5 mm length; ThermoFisher Scientific, #160454) was used to concentrate the sample ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a PepMap C18 trap column (300 µm x 5 mm, 5 µm particle size, Thermo Fisher) and separated on a 50 cm Easy-Spray column (ES803 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were loaded onto the trap column (C18 PepMap100, 5 μm particle size, 300 μm x 5 mm, Thermo Scientific) for 4 min at 18 μL/min ...
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bioRxiv - Bioengineering 2020Quote: ... FTIR spectra were captured in PBS at 500 μg/mL for each particle type utilizing a Nicolet 6700 FT-IR instrument (Thermo Scientific). Spectra were collected in the range of 3400 cm−1 to 400 cm−1 ...