Labshake search
Citations for Thermo Fisher :
1 - 50 of 2807 citations for 8 sec Butyl quinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... and tert-butyl hydrogen peroxide (ThermoFisher) were used as standards for the extra- and intracellular assays ...
-
bioRxiv - Microbiology 2023Quote: ... tert-Butyl hydroperoxide (tBOOH – Acros Organics) and diamide (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2022Quote: ... di-tert-butyl dicarbonate (Boc2O, 99%, Fisher Scientific) (1.6:1 to HA-TBA repeat unit ...
-
bioRxiv - Immunology 2021Quote: ... tert-butyl hydrogen peroxide was obtained from ACROS Organics; Oxidized and reduced L-Glutathione were obtained from Alfa Aesar ...
-
bioRxiv - Immunology 2023Quote: ... Di-tert-butyl peroxide (DTBP) crosslinker (#20665, Thermo Fisher Scientific) was added for 5 minutes at 37 °C to a final concentration of 2.5 mM ...
-
bioRxiv - Cancer Biology 2022Quote: ... and butylparaben (Butyl 4-hydroxybenzoate, BP; Acros Organics, CAT: 403571000). Chemicals were dissolved in ethanol (EtOH ...
-
bioRxiv - Biochemistry 2023Quote: ... methyl tert-butyl ether and 2-propanol were from Thermo Fisher Scientific (Pittsburg ...
-
bioRxiv - Cancer Biology 2021Quote: ... The fluorescent reaction was measured every 40 sec for 8 min and subsequent every 5 min for 6 times using a microplate reader (Thermo Scientific) with enzyme kinetics model ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were sonicated for ∼30 sec (10-sec pulse, 10-sec gap, 3x) using a sonicator (Fisher Scientific, VCX-130 Vibra-Cell Ultrasonic). The resulting lysed cells were centrifuged at 600rpm for 5 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... Tert-butyl hydroperoxide (TBHP, 70% solution in water) was obtained from Acros Organics and diluted in PBS ...
-
bioRxiv - Cell Biology 2024Quote: ... 3) Oxidative stress induction using 70 μM tert-Butyl hydroperoxide (Thermo Fisher 180345000) for 16-18 h ...
-
bioRxiv - Biochemistry 2021Quote: ... 56°C for 15 sec and 72°C for 10 sec) using Phusion polymerase (Thermo Scientific) and primers complementary to the constant annealing regions flanking the designed library sequences ...
-
bioRxiv - Cell Biology 2022Quote: ... The crude peptide was precipitated in tert-butyl methyl ether (TBME, ACROS Organics, AC378720025). After the supernatant was removed by centrifugation ...
-
bioRxiv - Genomics 2020Quote: ... 57°C for 30 sec and 72°C for 30 sec using AmpliTaq Gold® Polymerase (Applied Biosystems). The 7550-7555 product was DNA sequenced by Sanger technology at Eurofins Scientific ...
-
bioRxiv - Biochemistry 2023Quote: SEC separation was carried out using an Acclaim SEC-1000 7.8 × 300 mm (Thermo Fisher Scientific, Waltham, MA), a PolySep GFC-P 6000 7.8 × 300 mm (Phenomenex ...
-
bioRxiv - Biochemistry 2024Quote: ... Samples were further lysed via sonication (10 sec on 5 sec off, repeat 3x, ThermoFisher: FB120, 4°C) and protein was clarified by centrifugation at 21,000 x g for 5 min at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... Fractions were pooled and concentrated using a 100 kDa MWCO Amicon spin filter and subsequently purified on a GE AKTA Pure using an SRT SEC-1000 SEC column equilibrated in 1X Dulbecco’s phosphate-buffered saline (DPBS) (Gibco).
-
bioRxiv - Molecular Biology 2019Quote: ... homogenized through a 23G needle on ice, sonicated (BioRuptor Pico, 10x 30 sec ON, 30 sec OFF) and incubated with 2 U/ml Turbo DNase (Invitrogen) for 10 min at 37°C ...
-
bioRxiv - Genomics 2021Quote: ... Briefly, lysates were sonicated 12 times (15 sec on, 180 sec off) using a Dismembrator Model 500 sonicator (Fisher Scientific) at 30% output ...
-
bioRxiv - Immunology 2020Quote: ... SEC was performed in Ultimate 3000 (ThermoFisher, USA) system using 10 mM Disodium hydrogen phosphate (Wako ...
-
bioRxiv - Biophysics 2019Quote: ... Cleaved peptides were precipitated from ice-cold methyl tert-butyl ether (MTBE; Thermofisher Acros Organics, Geel, Belgium). After washing and collection by centrifugation crude peptides were dissolved in 20% (v/v ...
-
bioRxiv - Biophysics 2019Quote: ... Cleaved peptides were precipitated from ice-cold methyl tert-butyl ether (MTBE; Thermofisher Acros Organics, Geel, Belgium). After washing and collection by centrifugation crude peptides were dissolved in 20% (v/v ...
-
bioRxiv - Genetics 2019Quote: ... Lysate was then subjected to sonication for five minutes at 4°C (amplitude 20%, 10 sec pulses, 50 sec rest between pulses) using a sonicator (Fisher Scientific), and centrifuged at 21,000 x g for 15 min at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... 56°C for 15 sec and 72°C for 30 sec in the Applied Biosystems 7500 or QuantStudio5 Real-Time PCR instruments (ThermoFisher Scientific). For absolute quantification ...
-
bioRxiv - Bioengineering 2021Quote: ... Cell suspensions were lysed by sonication (10 cycles of 10 sec on, 10 sec off at 80% power) (Qsonica Sonicator Q125, Fisher Scientific). Soluble and insoluble fractions from each cell lysate were separated by centrifugation at 20,000xg for 40min at 4°C and used to determine the percent soluble protein as described in the text.
-
bioRxiv - Physiology 2020Quote: ... The supernatant was sonicated for a total of 5 min (10 sec on and 10 sec off for 10 min) on setting 9 of a model 550 Sonic Dismembrator (Fisher Scientific). The supernatant was spun again and transferred to a new tube avoiding precipitant ...
-
bioRxiv - Microbiology 2022Quote: ... 95°C 2 min, (95°C 15 sec., 60°C 20 sec.) x 40 cycles on a StepOnePlus Real-Time PCR system (Applied Biosystems). For a standard curve ...
-
bioRxiv - Immunology 2023Quote: ... 56°C for 15 sec and 72°C for 30 sec in the Applied Biosystems 7500 or QuantStudio5 Real-Time PCR instruments (ThermoFisher Scientific). For absolute quantification ...
-
bioRxiv - Cancer Biology 2023Quote: ... 95°C for 15 sec and ramp rate 0.075°C/sec) in a QuantStudio5 Real-Time PCR System (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2024Quote: ... 10 min at 95 °C followed by 40 cycles of 10 sec at 95 °C and 40 sec at 60 °C using the Quant 6 real-time PCR instrument (Applied Biosystems). Samples were considered positive for Giardia spp ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 μL of the sample were applied twice with a 30 sec interval and 0.5 sec blotting prior to the second application inside the chamber of VItrobot Mark IV (Thermo Fisher Scientific, USA) at 4°C and 92 % humidity ...
-
bioRxiv - Plant Biology 2020Quote: ... and the samples re-dissolved in 50 μL methyl tertiary butyl ether (MTBE) (Thermo Fisher Scientific, MA, USA), of which 1.0 μL was injected into a Waters Acquity Ultra-performance Convergence Chromatography (UPC2 ...
-
bioRxiv - Biochemistry 2023Quote: ... 5 mM disuccinimidyl suberate (DSS) or 2 mM tert-butyl disuccinimidyl phenyl phosphonate (tBu-PhoX)(Thermo Fisher Scientific) in DMSO was added to the solution and incubated for 1 hr at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... in an Acclaim SEC 300 (Thermo Fisher Scientific, USA) columns (4.6 × 300mm ...
-
bioRxiv - Plant Biology 2023Quote: ... SEC and SPY were cloned into pDONR207® (Invitrogen) using primers in Supplementary Table 1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 45 cycles of cyclic denaturation and annealing at 95°C for 15 secs and 60°C for 60 secs respectively were used in QuantStudio3 Real-Time PCR System (Thermo Fisher Scientific, Singapore). The miRNA expression levels were calculated using the 2-ΔΔCt method [38]and were normalized to the U6 expression) ...
-
bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 30 sec and extension at 60 °C for 30 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
-
bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 5 sec and extension at 58 °C for 34 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
-
bioRxiv - Plant Biology 2020Quote: ... The SEC column was a Biobasic SEC 300 (300 × 7.8; 5 µm particle size, 300 Å pore size; Thermo Fisher Scientific, Waltham, MA, USA), mounted with a pre-column in front (Biobasic SEC 300 pre-column ...
-
bioRxiv - Bioengineering 2020Quote: SEC-MALS was performed on a HPLC system (Thermo Fisher) connected to a light scattering detector (miniDAWN TREOS ...
-
bioRxiv - Genomics 2022Quote: ... 30 sec at 72°C) using Phusion polymerase (ThermoFisher Scientific) and primers complementary to the adapters ...
-
bioRxiv - Neuroscience 2020Quote: ... was added prior to sonication on the lowest power setting (Output power: 3-4 Watts; 3x for 10 sec on, 30 sec off; Fisher Scientific Sonic Dismembrator Model 100). The samples were incubated with 150 units micrococcal nuclease (Cell Signaling ...
-
bioRxiv - Genetics 2023Quote: ... 45 amplification cycles at 95°C for 5 sec and 60°C for 30 sec) in the Applied Biosystems 7300 fluorescence quantitative PCR system (Applied Biosystems, Foster City, CA, USA) according to the steps in the instruction book of SYBR PrimeScript RT-PCR kit (Takara) ...
-
bioRxiv - Bioengineering 2021Quote: ... The reaction was monitored using an Agilent 1100 HPLC system equipped with a MAbPac HIC-Butyl column (4.6 × 100 mm, 5 µm, Thermo Scientific). Elution conditions were as follows ...
-
bioRxiv - Biochemistry 2020Quote: ... N,N-dimethyl formamide (DMF) and water and HPLC grade methyl tert-butyl ether (MTBE) were obtained from Thermo Fisher used throughout ...
-
bioRxiv - Bioengineering 2020Quote: ... was analyzed using an Agilent 1100 HPLC system equipped with a MAbPac HIC-Butyl column (4.6 × 100 mm, 5 µm, Thermo Scientific). Elution conditions were as follows ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were sonicated for 5 sec and SYTOX Green (Invitrogen S7020) at 2 μM was added to the cells before analysis in a BD FACSCanto cell sorter.
-
bioRxiv - Cell Biology 2023Quote: EVs isolated by SEC were incubated with 20µM CFDA-SE (Thermofisher) for 2hrs at 37° as previously described (22) ...
-
bioRxiv - Biochemistry 2024Quote: ... The calibration curve was generated by analysing SEC standards (Thermo Scientific) under than same conditions as VC0430 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The lysate was then further decolorized by gently shaking against an equal volume of Methyl tert-Butyl Ether twice and then Hexanes (Fisher Scientific). The aqueous layer was then recovered ...