Labshake search
Citations for Thermo Fisher :
1 - 50 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... The concentrations of 2’3’-cGAMP was measured using 2’3’-cyclic cGAMP ELISA kit (Invitrogen) following the manufacturer’s instruction.
-
bioRxiv - Microbiology 2020Quote: ... Poly (I:C) and 2’-3’ cGAMP were delivered into cells using Lipofectamine 2000 (Thermo Fisher, USA) as described previously.
-
bioRxiv - Molecular Biology 2022Quote: ... A luminol-based enhanced chemiluminescence (ECL) detection kit (Thermo Fisher Scientific) was used to visualize bound antibodies ...
-
bioRxiv - Cell Biology 2019Quote: ... Detection of DNA synthesis in proliferating cells was determined based on the incorporation of 5-ethynyl-2′-deoxyuridine (EdU; Thermo Fisher Scientific) and its subsequent detection by a fluorescent azide through a Cu(I)-catalyzed [3 + 2] cycloaddition reaction (“click” chemistry ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... plates were treated with 2 μM of the CellEvent™Caspase-3/7 green detection reagent (Life Technologies, UK) for 60 minutes at 37 °C in the dark ...
-
bioRxiv - Cell Biology 2021Quote: FRET experiments were conducted in black plastic 96-well plates (Nunc) using a CLARIOstar plate reader (BMG LABTECH) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... #2: 5’-gatcccGATTACTCGGCCATGATCAAAgcttcctgtcacTTTGATCATGGCCGAGTAATCttt ttta-3’ and 5’-agcttaaaaaaGATTACTCGGCCATGATCAAAgtgacaggaagcTTTGATCATGGCCGAGTAATgg-3’ were purchased from Invitrogen. The DNA oligo pairs were annealed and inserted into pEntryCla12-chickU6 shuttle vector using BamHI/HindIII site.
-
bioRxiv - Biochemistry 2020Quote: ... and horseradish peroxidase-based chemiluminescence detection (SuperSignal® West Pico kit, Thermo Scientific).
-
bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... the CellEvent Caspase-3/7 Green Detection kit (Invitrogen) was used with propidium iodide as a viability marker ...
-
bioRxiv - Cancer Biology 2023Quote: While the FRET plates were being read in the Appliskan (Thermo Scientific), the imaging assay was prepared ...
-
bioRxiv - Microbiology 2020Quote: ... 5 µM CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher Scientific) were applied for monitoring effector caspase activation and 2.5 µM AlexaFluor647 hydrazide for detecting cell lysis.
-
bioRxiv - Microbiology 2020Quote: ... using the ZIKV-F2 (5’-CAGCTGGCATCATGAAGAATC-3’) and ZIKV-R1 (5’-CACTTGTCCCATC TTCTTCTCC-3’) primers for African strain detection (ThermoFisher SCIENTIFIC) or the ZIKV-F1 (5’-CAGCTGGCATCATGAAGAACC-3’ ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The plate was centrifuged at 2,000 × g for 1 min then lysate was transferred to a 384-well plate (30 μL × 2 TR-FRET replicates) using an adjustable electronic multichannel pipette (Matrix Equalizer, ThermoFisher 2231) and TR-FRET measurements were taken ...
-
bioRxiv - Biophysics 2021Quote: In vitro FRET experiments were conducted in black plastic 96-well plates (Nunc) with clear floors using a CLARIOstar plate reader (BMG LABTECH) ...
-
bioRxiv - Cell Biology 2022Quote: ... Luminescent ATP Detection Assay Kit (based on firefly’s luciferase / luciferin) was purchased from Molecular Probes.
-
bioRxiv - Neuroscience 2021Quote: ... we used a T7 endonuclease based method (GeneArtTM Genomic detection kit, Thermo Fisher Scientific, #A24372). Briefly ...
-
bioRxiv - Biochemistry 2024Quote: ... Luminescent ATP Detection Assay Kit (based on firefly’s luciferase / luciferin) was purchased from Molecular Probes.
-
bioRxiv - Molecular Biology 2019Quote: ... the PCR products obtained with 2 primers (AV48_SMCHD1_gPCR_F: 5’-AGGAGCGCGTTTGAATCGG-3’, AV47_SMCHD1_gPCR_R 5’-CTTCGCGTACCTGACACACAC-3’) were TOPO-cloned (Thermo Fisher, #450071) and sent for sequencing.
-
bioRxiv - Cell Biology 2020Quote: INS-1 832/13 cells were grown in 35-mm dishes and transfected at 50-60% confluency with FRET-based biosensors (2.5 μg plasmid DNA) using Lipofectamine 2000 (6 μl) (Invitrogen). 24 hours post-transfection cells were plated in μ-slide 8 well glass bottom chamber (Ibidi ...
-
bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
-
bioRxiv - Microbiology 2023Quote: ... 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated into a PCR by the use of a DreamTaqTM DNA Polymerase (Thermo Scientific™ ...
-
bioRxiv - Immunology 2020Quote: ... cells were treated with 2 mM caspase-3/7 detection reagent (Fisher Scientific) for 30 min ...
-
bioRxiv - Biochemistry 2022Quote: ... and 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher Scientific). Images were captured automatically every two hours for 48 hours using the IncuCyte™ S3 Live-Cell Analysis Instrument (Essen BioScience) ...
-
bioRxiv - Cell Biology 2023Quote: ... and treated with 2 µM Caspase-3/7 Green detection reagent (C10423, Invitrogen) along with the specified compounds ...
-
Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: ... Real Time qRT-PCR was performed using SYBR-green based detection on a Quant Studio 3 real time PCR system using PowerUP Syber green MasterMix (ThermoFisher). Primers for GAPDH and beta-actin were used as endogenous controls to which RNA levels of target genes were normalized to ...
-
bioRxiv - Immunology 2022Quote: ... Cells were counted again and resuspended in 3 × 106 cells/mL before adding CC4F-AM (LiveBLAzer FRET-B/G Loading Kit with CCF4-AM, ThermoFisher Scientific). Cells were incubated for 1 hour ...
-
bioRxiv - Genetics 2021Quote: ... The membrane was hybridized with a biotin-conjugated telomere probe (5’-biotin-CACACCCACACCCACACC-3’) and was imaged using a Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Scientific) and a Li-Cor C-DiGit Chemiluminescent Western Blot scanner.
-
bioRxiv - Neuroscience 2023Quote: ... plates were rinsed with 2-3 ml of PBS (Gibco, #10010023) and treated with 0,5 mL ReLeSR (Stemcell technologies ...
-
bioRxiv - Neuroscience 2021Quote: ... based medium with B27 (2%, Thermofisher) and GlutaMAX (2 mM ...
-
bioRxiv - Neuroscience 2023Quote: ... based media with B27 (2%, Thermofisher) and GlutaMAX (2 mM ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: The assay procedure is based on the manufacturer’s protocol for LanthaScreen™ TR-FRET Thyroid Receptor beta Coactivator Assay (Invitrogen, PV4686) with slight ...
-
bioRxiv - Immunology 2019Quote: ... 5’-CCCTACTGTATCCTCATG-3’/5’-CTTACCTCCTCTTCAATAGC-3’ PRKDC: 5’-GGGGCATTTCCGGGTCCGGG-3’/5’-TGCCCTGCCCCCCACTCTGC-3’ Amplicons were cloned using the Zero Blunt TOPO PCR Cloning kit (ThermoFisher), prepared as plasmids ...
-
bioRxiv - Developmental Biology 2019Quote: ... followed by ECL-based detection (Pierce ECL plus, Thermo Scientific, 32123). The following primary antibodies were used for Western blot analysis ...
-
bioRxiv - Genetics 2021Quote: ... DNA was quantified using fluorescence-based detection on a Qubit (Thermofisher). Whole genome sequencing libraries were prepared at The Centre for Applied Genomics (The Hospital of Sick Children ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then loaded with CCF4-AM for 2 hours at room temperature using the LiveBLAzer Fret-B/G Loading with CCF4-AM kit from Thermo Fisher. After 2 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... and loaded with the Fluorescent Dye-Based Rhod-3 AM following the manufactures’ instructions (Rhod-3 Calcium Imaging Kit, Cat.No. R10145; ThermoFisher scientific). Loading ...
-
bioRxiv - Immunology 2023Quote: ... a mouse IL-2 detection kit (Thermo Fisher #50-171-92) or mouse IFNγ detection kit (Thermo Fisher #88-7314-22 ...
-
bioRxiv - Microbiology 2021Quote: ... or with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) for 30 min at room temperature (1:150 in AnnexinV binding buffer-Biolegend) ...
-
bioRxiv - Cell Biology 2021Quote: ... The βarr1/2 siRNA (5’-ACCUGCGCCUUCCGCUAUG-3’) and a scrambled siRNA (control, 5’-UGGUUUACAUGUCGACUAA-3’) (Dharmacon) were transfected by RNAimax (Invitrogen) according to the instructions of the manufacturer ...
-
bioRxiv - Cell Biology 2021Quote: EdU (5-ethynyl-2′-deoxyuridine) detection was performed with the Click-iT EdU Alexa Fluor 555 Imaging Kit (Thermo-Fisher Scientific). EdU was diluted in DMSO to a final concentration of 10 mM ...
-
bioRxiv - Developmental Biology 2023Quote: ... NAE staining was followed by 5-ethynyl-2⍰-deoxyuridine (EdU) detection using Click-iT Edu Alexa Fluor 488 or 594 Imaging kits (Life Technologies) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... The modified nucleotide 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated by PCR using DreamTaq™ DNA Polymerase (Thermo Scientific™) ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were labeled with 5-ethynyl-2’-deoxyuridine followed by detection by click chemistry and then flow cytometry based on the instructions of the manufacturer (ThermoFisher, C01425).
-
bioRxiv - Cancer Biology 2023Quote: ... or STING (cat. no. PA5-23381, Invitrogen) was done overnight at 4°C ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... discoideum clones on SM/5 plates (2 g glucose (Fisher Scientific), 2 g Bacto Peptone (Oxoid) ...
-
bioRxiv - Immunology 2020Quote: Cell viability was estimated by a quantitative colorimetric assay described for human granulocytes which were based on metabolic reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Invitrogen) into coloured product formazan (Oez ...
-
bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
-
bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
-
bioRxiv - Cell Biology 2023Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...