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Citations for Thermo Fisher :
4451 - 4500 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Lysates containing Utrophin or α-actinin were loaded on a column packed with His-Pur Ni-NTA resin (ThermoFisher Scientific, Waltham, MA). The column was washed with 0.6 M NaCl ...
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bioRxiv - Developmental Biology 2024Quote: pMT-Reps-Flag and pMT-Reps-HA were generated by cloning Reps-RA isoform from FMO04419 (DGRC Stock 1612020) into pMT-V5-His vector (Invitrogen cat#V412020). pMT-Mnb-V5 was generated by cloning Mnb-RH isoform from FMO12028 (DGRC Stock 1639183 ...
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bioRxiv - Cancer Biology 2021Quote: The BCR-ABL-positive B-cell leukemia cell line BV173 (DSMZ, Braunschweig, Germany) was cultivated in RPMI1640 Supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco).
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bioRxiv - Cell Biology 2020Quote: ... the cell viability was determined using a micro plate reader (Thermo Scientific Multiskan FC, Thermo Fisher Scientific Oy, Ratstie, Finland) according to the manufacture’s protocol of CCK-8 ...
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bioRxiv - Cell Biology 2020Quote: HELA cells were plated in a 12-well plate in DMEM+ 10%FCS and transiently transfected using Lipofectamine3000 (Thermofisher, L3000001). Each transfection consisted of 250 ng expression plasmid (pcDNA3-control (Addgene ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Intestinal pieces were then placed into 10 mL RPMI 20% FCS with 0.5 mg/mL Collagenase Type I (Gibco #17100017) and 0.1 mg/mL DNase I (Sigma #DN25 ...
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bioRxiv - Microbiology 2022Quote: Unspecific Fc binding was blocked for 15 minutes on ice with unconjugated anti-CD16/CD32 (clone 93; Thermo-Fisher Scientific). Cells were subsequently fluorescently labeled for 30 minutes on ice with the following antibodies and reagents ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were excluded using ThermoFisher LIVE/DEAD Fixable Aqua Dead Cell Stain and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Absolute cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
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bioRxiv - Immunology 2019Quote: ... ATCC CRL-3216) cells were maintained in DMEM-Glutamax supplemented with 10% heat-inactivated fetal calf serum (FCS, ThermoFisher Scientific) and Penicillin-Streptomycin (PS ...
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bioRxiv - Immunology 2019Quote: ... whole stomachs were finely minced in disassociation buffer (2% FCS in Hank’s Balanced Salt Solution without calcium and magnesium (Life Technologies) and 5 mM EDTA ...
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bioRxiv - Developmental Biology 2019Quote: ... The remaining tissue was let to sediment and the supernatant was collected in a tube containing the same volume of 10% FCS (Gibco)-HBSS+/+ and kept on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... grown in DMEM supplemented with 10% FCS and neurospheres (RNS, enriched in cancer stem cells) grown in DMEM/Ham’s F12 GlutaMAX (Life Technologies) supplemented with B27 and N2 additives (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... cell number enumerated with a hemocytometer and resuspended in 0.5 ml PBS supplemented with fetal calf serum (FCS, ThermoFisher Scientific) to a final concentration of 1 × 103 cells/ml in each well of a 24 well plate ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were cultured in DMEM (Biowhitaker) supplemented with 10% fetal calf serum (FCS) (v/v) and 1% penicillin/streptomycin (GIBCO). Inducible cell lines were selected on 5 μg/ml blasticidin (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... were cultured and maintained at 37 °C in a humidified atmosphere of 5 % CO2 in air in Dulbecco’s Modified Eagle’s Medium (DMEM) + GlutMAXTM-1 containing 10 % Fetal calf serum (FCS) and 1% Penicillin-Streptomycin (10 000 U/ml) (Gibco Life Technologies ...
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bioRxiv - Immunology 2021Quote: Released tissue cells were stained in aliquots of 1 ×106 cells/100 μL of PBS with 2% fetal calf serum (FCS, GIBCO) and 0.1% NaN3 for 30min on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... for the last 30 min of the drug treatment in L-methionine-depleted medium containing 5% dialyzed fetal calf serum (FCS; Gibco). Harvested cells were washed with PBS ...
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bioRxiv - Bioengineering 2020Quote: ACE-Fc fusion proteins and the SARS-CoV-2 RBD were expressed in suspension-HEK293F cells grown in FreeStyle media (Gibco). Transfections were done using 40 kDa polyethyleneimine (PEI-MAX ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with a plasmid coding for SARS-CoV-2 RBD or ACE2-Fc and were purified on Ni-NTA resin (Invitrogen) or Protein A resin (Cytiva) ...
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bioRxiv - Cancer Biology 2020Quote: ... BALB/c-derived A20 B lymphoma cell line was obtained from the American Type Culture Collection (Rockville, MD) and maintained in RPMI 1640 supplemented with 10% FCS (GIBCO). For in vivo experiments A20 cell lines were injected i.v ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice with ice cold Cytoperm reagent and re-suspended in 1% fetal calf serum (FCS) in PBS (Invitrogen, 14190). Cells were subsequently analyzed on a CYAN flow cytometer (Beckmann Coulter).
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bioRxiv - Microbiology 2020Quote: ... Cells were maintained at 37°C in the presence of 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum (FCS) and 1% penicillin–streptomycin (fresh medium) (all from Invitrogen). Cells were used between 10 and 35 passages ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Neuroscience 2020Quote: ... After washing chase medium (DMEM containing 10% FCS, Glutamax, P/S, 1 mg/mL methionine and 1 mg/mL cysteine (Gibco)) was added to cells and incubated for different time points ...
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bioRxiv - Immunology 2021Quote: ... NK cells were then expanded for 6 days with hIL-15 50ng/ml (Miltenyi #130-095-765) in RPMI 20% FCS supplemented with L-glutamine (2 mM; Gibco), penicillin/streptomycin (100 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... splenic NK cells were isolated and suspended in NK cell medium (phenol-red free RPMI 1640 containing 10% FCS, non-essential amino acids, L glutamine and sodium pyruvate, all from Gibco). The indicated target cells were labelled with 15μM Calcein-AM (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The overnight inoculum was diluted 1:33 in infection medium (DMEM + 10% FCS + HEPES (Life Technologies, cat. number: 15630-056)) to obtain OD600=1 after 3 h of incubation to give 1.5 × 109 bacteria/ml ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed three times for 5 min and incubated with 1:3000 dilution of horseradish peroxidase-conjugated Goat anti-mouse IgG Fc secondary antibody (Invitrogen) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... University of Bristol) were grown in DMEM supplemented with 8% FCS and 8% (v/v) tryptose phosphate broth (Life technologies). Penicillin and streptomycin (90 IU/ml ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Immunology 2022Quote: ... RPMI-1640 medium supplemented with 10% heat inactivated FCS and final concentrations of 100 U/mL penicillin and 100 μg/mL steptomycin and 2mM L-Glutamine (Gibco). Cells were incubated with OVA peptide (323-339 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal calf serum (FCS) (Thermo Fisher Scientific, Hamburg, Germany), 100 IU/ml penicillin and 100 μg/ml streptomycin (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... and cells were stained with Fixable Viability Dye (eBioscience) and primary antibodies in the presence of Fc block in flow buffer (DPBS [Gibco] supplemented with 2% FCS ...
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bioRxiv - Immunology 2020Quote: ... Biopsies were washed in PBS and transferred into wells containing RPMI-1640 + 10% FCS + 20 μg/mL G418 (Thermo Fisher) + 20 U/mL Pen/Strep and cultured for 24 hours.
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bioRxiv - Developmental Biology 2021Quote: ... 4° C following by re-suspending the cells in 0.5-1 of ml cell isolation media (2% FCS, DPBS:HBSS=1:1 and 1mM SYTOX Blue Dead Cell stain (ThermoFisher Scientific). Cells were imaged before FACS and after FACS to confirm successful purification of GFP+ cells.
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bioRxiv - Cell Biology 2021Quote: ... BSF RNAi cell lines were cultured in HMI-9 medium 47 supplemented with 10% (v/v) low-tetracycline foetal calf serum (FCS; Gibco) at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen ...
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bioRxiv - Cancer Biology 2020Quote: ... The CH12.F3 mouse B lymphocyte cell line (a generous gift from T. Honjo (Kyoto Univ.)) was cultured in RPMI/10% FCS/5% NCTC-109 (Invitrogen)/1X Penn-Strep/50μM αMeOH ...
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bioRxiv - Microbiology 2020Quote: ... once and then 100 μl of 1:300 diluted secondary antibody (ThermoFisher Cat # A-21091 for Fc-tag and ThermoFisher Cat # MA1-21315-647 for polyhistidine-tag ...
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bioRxiv - Cancer Biology 2019Quote: ... All media were supplemented with 10% Foetal Calf Serum (FCS) (First Link, Birmingham, UK) as well as 100 units/mL penicillin and 100 μg/mL streptomycin (Gibco™ through Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Up to 5×106 isolated cells were incubated for 30 min at 4 °C with anti-CD16/CD32 (Fc receptor) clone 93 (Invitrogen) to block non-specific binding and with fixable viability dye eFluor455UV (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... Blots were imaged using the LiCor Odyssey® Fc system after treatment with ECL Western Blotting-Substrat (Thermo Fisher, 32209).
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bioRxiv - Immunology 2022Quote: ... femurs and tibia of mice were flushed with complete medium (RPMI-1640 and 10% heat inactivated fetal calf serum (FCS) (Gibco). Single cell suspensions were incubated with 5 mL of red blood cell (RBC ...
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bioRxiv - Immunology 2022Quote: Surface antibody staining was performed in PBS containing 5% FCS and 1mM EDTA and using a Fixable Aqua Dead Cell (Invitrogen) to determine viability ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: 3T3-F442A preadipocytes were grown and differentiated in a modified DMEM designed for isotope labeling in cell culture [Sigma Aldrich (Saint Louis, MO, USA)] supplemented with 10% dialyzed FCS (GIBCO-Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... HT29 and LIM1215 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FCS (all from Life Technologies). Cells were maintained in a tissue culture incubator (Sanyo Europe ...
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bioRxiv - Cell Biology 2020Quote: ... Commercial colloidal force probes (CP-qp-CONT-BSA-A, NanoAndMore USA) were passivated with heat-inactivated fetal calf serum (FCS, Invitrogen) for 1h at room temperature to avoid non-specific adhesion of the bead to the cells ...
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bioRxiv - Pathology 2020Quote: ... cells were first synchronized overnight in 1% FCS DMEM and then cultured for 14 days in 10% FCS DMEM and stimulated daily with IL-1β (1 ng/ml; Invitrogen) or IL-17 at (2.5 ng/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Two x 106 cells/well of a 6-well plate were seeded in DMEM containing 10% FCS (Invitrogen, Carlsbad; USA), 1% HEPES (Carl Roth ...