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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Recombinant Human PENK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... The protein was expressed in Sf9 insect cells using a recombinant baculovirus system (Life Technologies). The protein was purified on a cobalt resin column and further purified by gel filtration to ensure the removal of monomeric and aggregated protein.
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bioRxiv - Biochemistry 2020Quote: ... The recombinant PfAdoMetDC protein was purified using the Strep-Tactin Sepharose column (Invitrogen, California, USA) as described previously (29 ...
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bioRxiv - Immunology 2021Quote: ... Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) and the purity was assessed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Cell Biology 2021Quote: Binding of recombinant proteins to pre-spotted PIP and membrane lipid strips (Thermo Fisher; P23751) was performed according to (Dowler et al. ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp ...
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bioRxiv - Immunology 2020Quote: ... 100uL of recombinant protein at 0.5 ug/ml was coated on Maxisorp™ plates (Nunc) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant ACE2-Fc protein which was obtained from ExpiCHO™ expression system (Thermo Fisher) was 5-fold serially diluted in DMEM medium supplementing 10% FBS (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant hACE2-Fc protein was biotinylated using the EZ-Link sulfo-nhs-biotin kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Integrin-β2 recombinant protein complexes were immobilized on Maxisorp Immuno plates (ThermoFisher, 12-565-135) and used for positive binding selections with library phage pools that were first exposed to neutravidin coated wells to deplete nonspecific binders ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Immunology 2024Quote: ... recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: Recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: The recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as reported previously (25 ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Cell Biology 2023Quote: Recombinant ectodomain proteins were generated by transfection of HEK293T cell cultures using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... then recombinant protein purified from the soluble fraction using HisPur Ni-NTA columns (Thermo Scientific). Purified proteins were dialyzed against EMSA buffer ...
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bioRxiv - Immunology 2023Quote: ... ELISAs were conducted with recombinant full proteins coated overnight in Nunc MaxiSorp 384 plates (ThermoFisher) (2µg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... Hu5F9 and TJC4 were then purified using recombinant Protein A-Sepharose 4B (Thermo Fisher Scientific) buffer exchanged into PBS and concentrated using Amicon Centrifugal Filters (Millipore Sigma) ...
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bioRxiv - Genetics 2023Quote: ... Protein concentration of recombinant SIRT5 was subsequently determined by Bradford assay (#23200, Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... the recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as previously detailed (56 ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant proteins were generated using the Bac-to-Bac Baculovirus Expression Systems (Thermo Fisher Sc.). These recombinant proteins were then immobilized on anti-HA magnetic beads and subsequently incubated ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) plus 2% penicillin-streptomycin (Gibco) at 37°C in 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 in EMEM (ATCC) supplemented with 0.01 mg/mL human recombinant insulin (Gibco) and 10% (vol/vol ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in six-well plates coated with recombinant human vitronectin (VTN-N, Thermo Fisher Scientific, A14700) in Essential 8 medium (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: Levels of total APOE protein were analysed by Human ELISA Kit from Invitrogen (Apolipoprotein E Human ELISA Kit). Levels of Aβ were analysed by ELISA (Human β Amyloid 42 ELISA Kit Wako high sensitive #298-64401 and Human β Amyloid 40 ELISA Kit Wako #298-64601 ...
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bioRxiv - Bioengineering 2021Quote: ... The positive control consisted of 100 – 500 ng recombinant Protein A (ThermoFisher Scientific, Santa Clara, CA). The negative control consisted of ~2 μg wt-TMV from purified N ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified cell supernatant containing recombinant mAb was passed over Protein A Agarose resin (Thermo Fisher Scientific). Protein A resin was extensively washed with 25 mM Phosphate pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant Cripto protein or transfected with Cripto expression plasmid (pcCripto) using Lipofectamine2000 (Life Technologies, Carlsbad, USA) according to the manufacturer`s instructions ...
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bioRxiv - Pathology 2022Quote: TRP120 and TRX recombinant proteins were desalted using Zeba spin desalting columns (Thermo Fisher Scientific, MA) as indicated by the manufacturer protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 µL of the cleared lysates were added with 20 µL Recombinant Protein G Agarose (Invitrogen) and rotated for 30 min at 4 °C to preclear ...