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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Oropouche Virus Gn Protein Human Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... biotin anti-FC (Thermo Fisher, A18821), by mixing the nanovial concentrate and the antibody solution at a 1:10 ratio and incubating for 10 min ...
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bioRxiv - Immunology 2021Quote: ... with 5% FCS (Thermo Fisher Scientific). The dilutions were subsequently mixed with equal volumes of 4000 TCID50/ml SARS-CoV-2 (200 TCID50/ well ...
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bioRxiv - Immunology 2020Quote: ... containing 10% Heat Inactivated FCS (Invitrogen) and 1% Penicillin-Streptomycin (Merck) ...
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bioRxiv - Immunology 2020Quote: ... anti Fc primary antibody (ThermoFisher, USA), Alexa Fluor® 594 conjugate secondary antibody (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... supplemented 10% FCS (Thermo Fisher Scientific) and 1 % ampicillin at a cell density of 3×106/mL per condition ...
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bioRxiv - Immunology 2022Quote: ... and 1:200 Fc block (Invitrogen) in 1×PBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... medium supplemented with 10% FCS (Invitrogen), 100 µg/ml streptomycin and 100 U/ml penicillin (Sigma) ...
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bioRxiv - Genomics 2024Quote: ... both supplemented with 10% FCS (Invitrogen), 2 mM glutamine (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... 10% Fetal Calf Serum (FCS, Gibco) and 0.05 mM β-mercapto-ethanol ...
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bioRxiv - Immunology 2022Quote: ... + 1% FCS + 5 mM EDTA (Gibco)) and incubated with 5μl TruStain FcX receptor blocking solution (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FCS (Gibco, 10270). Stable cell lines were created either using Epac-SH250 biosensor or using Epac-SH201 biosensor18 ...
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bioRxiv - Immunology 2023Quote: ... A Fc-XP column (ThermoFisher, 494371201) containing anti-IgG-CH3 matrix which binds to the CH3 region (on Fc region ...
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bioRxiv - Neuroscience 2024Quote: ... 10% fetal calf serum (FCS) (Gibco), 1× penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein bands were visualized using a LI-COR Odyssey FC Imaging system and Super Signal West Pico Chemiluminescent Substrate (Thermo Fisher). Western blots were quantified using Image Studio Software ...
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bioRxiv - Immunology 2019Quote: ... The idiotype antibodies and Fc-fusion proteins were conjugated in house with Dylight488 and/or 650 antibody labeling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and Rspo3-Fc Fusion Protein Conditioned Medium (Immunoprecise, #R001-100ml, 2%)] and mixed with 50 µl Matrigel (Fisher Scientific, #CB-40230C). Human alveolar organoid media was refreshed every 3 days until the end of the experiment ...
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Evolutionary stasis of an RNA virus indicates arbovirus re-emergence triggered by accidental releasebioRxiv - Evolutionary Biology 2019Quote: ... and virus isolates using Trizol LS® (Invitrogen, USA) and purified using Direct-zol RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... or Sendai virus system Cytotune 2.0 (Thermo Scientific #A16517). Resulting iPSCs were clonally picked and expanded in mTESR (Stemcell Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The virus inoculum was prepared in OPTI-MEM (Gibco), and cells were incubated in SMEM at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted virus was resuspended in PBS and TRIzol (Invitrogen) extracted ...
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bioRxiv - Microbiology 2022Quote: ... and from virus in supernatant using Trizol LS (ThermoFisher). A one-step qRT-PCR kit with SYBR green from Agilent was used ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were diluted in OptiPRO SFM (ThermoFisher Scientific) serum-free medium according to the desired MOI ...
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bioRxiv - Microbiology 2020Quote: ... The virus pellet was washed in HBSS (Thermo Fisher), and centrifuged at 100,000 × g (Beckman SW 32 Ti rotor ...
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bioRxiv - Immunology 2022Quote: ... and Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen), followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Immobilised virus was fixed with 4% formaldehyde (Thermo Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: Virus stocks were prepared by transfecting 293FT cells (Invitrogen) seeded in 10 cm cell culture dishes with the viral constructs described above ...
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bioRxiv - Microbiology 2024Quote: ... and TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Reactions were set up according to the manufacturer’s protocols using a 10 µl reaction volume and 25 ng of total RNA template ...
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bioRxiv - Cancer Biology 2024Quote: ... virus was prepared in high-glucose DMEM (Gibco, #11965092) supplemented with 10% FBS and 1% penicillin–streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and primer/probes targeting NS3 primers/probe (IDT)11 ...
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bioRxiv - Genomics 2024Quote: The pseudotyped virus was generated from HEK293T cells (Invitrogen) by co-transfection with human immunodeficiency virus backbone plasmids expressing firefly luciferase as described previously ...
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bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
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bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked by incubating cells with 1:50 anti- mouse CD16/CD32 (Fc block, Invitrogen) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and H1N1/2009 influenza virus strains were prepared by overnight incubation of the virus strains with 0.1% v/v β propiolactone (Acros Organics, Geel, Belgium) under continuous rotation at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... were combined with 0.36 μg of recombinant human protein CDK1/Cyclin B (Thermo Fisher, PV3292), 1x Kinase Buffer (Cell Signaling ...
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bioRxiv - Immunology 2022Quote: All experiments were performed at 4°C using ProtoArray Human Protein Microarrays (Thermo Fisher Scientific). Microarrays were blocked for 1 h in blocking solution (Thermo Fisher) ...
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bioRxiv - Bioengineering 2022Quote: ... the medium was supplemented with Human Interferon-gamma Recombinant Protein (IFN-γ) (ThermoFisher, Cat. #. PHC4031) at a concentration of 50 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: Reconstitute Human FGF-basic (FGF-2/bFGF) Recombinant Protein (here we use ThermoFisher 13256-029). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... and 100U/ml PeproGMP™ Human IL-2 Recombinant Protein (Thermo Fisher; 200-02-1MG). The T cell culture was transferred to G-Rex bioreactor vessels (Wilson Wolf ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Developmental Biology 2019Quote: CIL in single cells encountering cadherin coated beads used cdh3-coated beads prepared by binding purified cdh3-fc (gifted from Dr. Douglas W. DeSimone and Dr. Barry. M. Gumbiner) to Protein A/G coated beads (53132, Thermo Fisher Scientific) (Chappuis-Flament et al. ...
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bioRxiv - Microbiology 2022Quote: ... 4a3A cells were maintained at 27°C without CO2 in Insect-XPRESS™ Protein-free Insect Cell Medium complemented with heat-inactivated Fetal Calf Serum (FCS) (#A3840002 ThermoFisher Scientific). C6/36 cell line (ATCC ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Molecular Biology 2019Quote: 2.5D-Fc and 2.5F-Fc fusions were labeled with the succinimidyl ester derivative of Alexa Fluor 488 (Invitrogen) according to the manufacturer’s protocol ...