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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... until being separated isocratically by a reversed-phase C18 column with a flow rate of 0.6 mL/ min using a Dionex Ultimate 3000 HPLC system (pump ISO-3100SD, Thermo Scientific, Bannockburn, IL). Electrochemical detection was achieved using a CoulArray model 5600A coupled with a guard cell (model 5020 ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were resuspended in 1.25 mL radioimmunoprecipitation assay buffer consisting of 12.5 μL of protease inhibitor (Halt Protease Cocktail Inhibitor, Thermo Fisher Scientific, Rockford, IL) to prevent enzymatic degradation upon cell lysis ...
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bioRxiv - Molecular Biology 2020Quote: EndoC-βH1 cells were treated with DIMT1 siRNA and lysates were isolated using a proprietary lysis buffer (Pierce, Thermo Scientific, (Rockford, IL). Co-IP was performed at 4°C unless otherwise indicated ...
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bioRxiv - Microbiology 2020Quote: ... The resulted peptides were cleaned up in a HyperSep filter plate with bed volume of 40 μL (Thermo Fisher Scientific, Rockford, IL). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transferred to medium containing CSS and transfected with 35-100 nM siRNA using the Lipofectamine RNAiMAX reagent (Invitrogen, Rockville IL) according to the manufacturer’s directions for a duration of 48-72 hours.
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bioRxiv - Bioengineering 2022Quote: ... CCL5 or IL-2 was performed according to the protocol of eBioscience™ Intracellular Fixation & Permeabilization Buffer Set (Cat.88-8824-00, Thermo Fisher). Briefly ...
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bioRxiv - Microbiology 2022Quote: Primary human CD4+ T cells were incubated with 20U/ml interleukin-2 (IL-2) alone or in combination with α-CD3/CD28 antibody-coated beads (100 μL/30 x 106 cells) (Gibco/Thermo Fisher) for 72 hours prior to infection with 100ng of p24 equivalent of pseudotyped pMorpheus-V5 ...
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bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...
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bioRxiv - Immunology 2022Quote: ... 1% MEM NEAA and 50 Units/mL recombinant human IL-2 (Myltenyi; #130-097-745) with Dynabeads™ Human T-activator CD3/CD28 (Gibco, #11132D) the same day of the purification (day 0 ...
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bioRxiv - Neuroscience 2021Quote: ... at room temperature for 5 min and then the slides were mounted using Fluoromount(tm) (Life Technologies-Thermo scientific, Rockford, IL, USA) as a mounting medium.
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bioRxiv - Immunology 2021Quote: ... for Tcaim and Ubc or custom designed primer and probes for Il-2 and Ifn-γ (Eurofins Genomics) and TaqMan Universal PCR Master Mix (Thermo Fisher) on a 7500 Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) with 1 mM phenylmethylsulphonyl fluoride (PMSF, USB Corporation, Cleveland, OH) and Pierce protease inhibitors with EDTA (ThermoFisher Scientific, Rockford, IL). Bacteria were lysed in a French pressure mini-cell by three cycles of 9,000 p.s.i ...
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bioRxiv - Immunology 2022Quote: ... fumigatus strains Af293 and CEA10 were grown at 37℃ for 5 days on Sabouraud’s Dextrose Agar (SBD; Difco, Fisher Scientific, Rockford, IL) or for 3 days on glucose minimal media slants (GMM) ...
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bioRxiv - Immunology 2022Quote: ... washed and added at 103 cells per well to various ratios of effector T cells (CTC or UTC population after overnight incubation with IL-15) in 96 well V-bottomed plates (NUNC, Thermo Fisher Scientific). After 4 hours of co-incubation ...
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bioRxiv - Synthetic Biology 2020Quote: 90 μL CFE semi-continuous reactions were performed using 3.5 kDa MWCO 96-well plate dialysis cassettes (Thermo Scientific, Rockford, IL, USA) in 2 mL microcentrifuge tubes with 1.4 mL dialysis buffer solution ...
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bioRxiv - Immunology 2019Quote: ... a 20X IL-26 human TaqMan® probe was used (Hs00218189_m1) with 2X TaqMan® Fast Advanced Master Mix (both from ThermoFisher Scientific). OSM and HEBGF cDNA quantification was performed with Roche ® hydrolysis probes (OSM ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were cultured at 37 °C, 5% CO2 in RPMI medium 1640 (Pierce, Rockford, IL) with 10% dialyzed fetal bovine serum (Invitrogen, Carlsbad, CA) and 1% penicillin/streptomycin for at least five passages to completely label the proteome with stable isotope labeled heavy Arg and Lys for three-state SILAC experiments ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated in PBS plus 0.5% Triton X-100 and Alexa Fluor 568-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... at room temperature for 5 min and then the slides were mounted using Fluoromount(tm) (Life Technologies-Thermo scientific, Rockford, IL, USA) as a mounting medium.
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bioRxiv - Developmental Biology 2021Quote: ... and frozen overnight at -80 °C in a NALGENE Cryo 1 °C freezing container (5100-0001, ThermoFisher Scientific, Hanover Park, IL, USA) filled with isopropanol (423830025 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 75% methanol in 1x PBS and three times in 100% methanol (A412-4, Fisher Chemical, Fisher Scientific, Hanover Park, IL, USA). Samples were stored at -20 °C in 100% methanol until processing.
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bioRxiv - Microbiology 2020Quote: ... Japan were cultured at 37 °C in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque, Japan) supplemented with 5% heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific, Rockford, IL) and antibiotics (10 units/mL penicillin and 10 μg/mL streptomycin ...
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bioRxiv - Genetics 2019Quote: ... The ovarian tissues were homogenized with M-PER Mammalian Protein Extraction Reagent (Pierce Biotechnology, Rockford, IL, USA) supplemented with Halt TM Protease Inhibitor Cocktail (Thermo Fisher Scientific). Following centrifugation (16,000 g ...
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bioRxiv - Developmental Biology 2022Quote: ... They were then washed briefly with PST and incubated in secondary antibodies and Hoechst 33342 (1:100,000; Thermo Fisher, Rockford, IL, USA) for DNA staining overnight at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... They were then incubated with primary antibody rabbit polyclonal anti-claudin-1 (Catalog# 51-9000; RRID: AB_2533916; final concentration, 1:100; Invitrogen, Rockford, IL, USA) overnight in a humid chamber at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and intracellular markers were stained with monoclonal antibodies for 45 minutes at 4°C with FoxP3 (FJK-16S) and IL-10 (JES5-16E3) (All antibodies were from Thermo Fisher Scientific). Cells were resuspended in PBS with 2% NCS and 2 mM EDTA ...
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bioRxiv - Physiology 2022Quote: ... MA) or 1 mM caffeine were used to evoke Ca2+ transients from fluo-4AM or rhod-2AM (Thermo Scientific, Rockford, IL, USA) loaded myocytes ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed with PBS lacking calcium and magnesium and lysed with IP Lysis buffer (Thermo Fisher Scientific, Rockford, IL, USA) supplemented with protease inhibitor cocktail kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Primary or secondary antibodies were purchased from the following vendors: rabbit anti-human ROBO1 (PA5-29917, Thermo Fisher Scientific, Rockford, IL, USA); rabbit anti-human ROBO1 (HPA052968 ...
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bioRxiv - Immunology 2024Quote: ... SuperSignal™ West Femto Maximum Sensitivity Substrate and Halt™ Protease and Phosphatase Inhibitor Cocktail were purchased from Thermo Scientific (Rockford, IL). Chemical inhibitors ...
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bioRxiv - Immunology 2023Quote: Ex vivo isolated and rested CD8+ T cells or transduced T cells from splenocytes were incubated with R10 (RPMI 1640 + 10% FBS + 1% penicillin/streptomycin + L-glutamine + 20 U/ml of IL-2; Gibco, Thermo Fisher Scientific) alone or R10 containing PMA/ionomycin for two hours ...
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bioRxiv - Evolutionary Biology 2023Quote: Pellets were thawed on ice and resuspended in 20 mL of HisTrap Binding Buffer (50 mM NaH2PO4, pH 7.4, 300 mM NaCl, 10 mM imidazole) and 2 µL Pierce Universal Nuclease (Thermo Scientific, Rockford, IL). Once resuspended ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4 or PD-1) were suspended in IL-2 medium at 37°C containing either 65nM LysoTracker® Deep Red (ThermoFisher Scientific) or 25nM MitoTracker™ Red CMXRos for 1h or 30min ...
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bioRxiv - Physiology 2023Quote: ... Samples were then washed in PBS (3 x 5 minutes in PBS at room temperature) and mounted in ProLong Gold (Invitrogen, Rockford, IL). For STochastic Optical Reconstruction Microscopy (STORM) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation at 17,000 xg for 5 min and total protein was quantified in the supernatant using the BCA protein assay (Thermo Scientific, Rockford, IL). SDS loading buffer (final concentrations of 100 mM Tris pH 6.8 ...
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bioRxiv - Immunology 2023Quote: ... Quantitative analysis of IL-6 and IFNB expression in THP1 dual reporter cells was performed by StepOne Real-Time PCR Systems (Thermo Fisher Scientific) by using 18S rRNA for normalization ...
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bioRxiv - Molecular Biology 2024Quote: ... were isolated from mice tibias and femurs as described previously29 and cultured for 7 days in Iscove’s Modified Dulbecco’s Medium supplemented with 10% FCS (Thermo Scientific, Rockford, IL, USA), penicillin-streptomycin (100 U/mL and 100 μg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Cat#CL-0190) and cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (FBS) (Thermo Scientific, Rockford, IL, USA), 100 U/mL penicillin (Solarbio ...
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bioRxiv - Cell Biology 2024Quote: ... 35 cycle PCR reactions with an input of 100 ng of gDNA and an annealing temperature of 58°C were performed using primers flanking the sgAscl1 edit site and KAPA mouse genotyping kit following manufacturer protocols (Fisher Scientific, #50-196-5243; Supplementary Table 11). A PCR product of 170 bp was purified using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were collected following a 48h incubation at 37°C and IFNγ and TNFα levels were measured by ELISA (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2020Quote: ... Blots were developed using Amersham ECL Prime Western Blotting Detection Reagent (RPN2232) or SuperSignal ELISA Femto Maximum Sensitivity Substrate (Thermo Scientific, Ref: 37075, Lot: UO282555) and the resulting chemiluminescence imaged with an Alpha Innotech MultiImage Light Cabinet (Alpha Innotech ...
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bioRxiv - Immunology 2020Quote: ... were diluted in 50 mM carbonate buffer to a concentration of 1 μg/ml and coated at 50 ng/well on 96-well ELISA plates (MaxiSorp Nunc, Thermo Fisher Scientific K.K., Japan) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound target-Fab complexes were incubated with an HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Formalin-killed N61 in PBS was dried in an oven at 5×106 cells/well in 96-well ELISA plates (C96 Maxisorpcert, Nunc-Immuno Plate, Thermo Fisher Scientific) overnight at 37°C ...
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bioRxiv - Plant Biology 2024Quote: Coating: Prepared sample were added to the selected ELISA-suitable antigen-binding 96-well microtiter plate (CORNING COSTAR 9018; Thermofisher scientific Ref: 44-2504-21). The first two columns contained the peptide standard ...
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bioRxiv - Molecular Biology 2020Quote: ... INS1 cells labeled with Bodipy C12 were also immunostained with mouse anti-HSP60 antibody (66-41-1-lg, Protein Tech) and Alexa Fluor 488 goat anti-Mouse IgG (A11017, Invitrogen) both at 1:300 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Blots were developed with a mouse polyclonal anti-SmHDAC8 antibody (1/1000) or mouse polyclonal anti-SmRho1 antibody (1/1000) and peroxidase coupled anti-mouse secondary antibody (1/50 000; Invitrogen). Detection was carried out by chemiluminescence using SuperSignal™ West Dura Extended Duration Substrate (Thermo Scientific ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: Mouse forebrains fractions or HEK293T cell lysates were incubated with either mouse anti-CaMKIIα (Thermo Fisher Scientific catalog #MA1–048), rabbit anti-Shank3 (Bethyl catalog #A304-178A) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal anti-Mcm3 antibody (M038-3; MBL) and anti-mouse IgG conjugated to Dynabeads M-280 (Thermo Fisher Scientific) were used as the primary and secondary antibodies ...