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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Recombinant Goat VEGFA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 Spike RBD 319-541 recombinant protein (OBA0101, Ovodan Biotech) was expressed in 25 mL culture using the Expi293F expression system (#A14635; ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
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bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).
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bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Biochemistry 2023Quote: Recombinant trimeric spike proteins (5 µg/mL) were plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then transfected overnight with 100 pmol of tracrRNA and crRNA complexed with 4 pmol recombinant cas9 protein using RNAiMAX (Thermo Fisher). Medium was then replaced and cells were grown for 48 h before assessing fluorescence and sorting.
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... 25ng/mL recombinant murine EGF (Gibco), 50ng/mL recombinant human HGF (Peprotech) ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... and RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... we added recombinant mouse IFNγ (ThermoFisher), 2-deoxyglucose (Abcam) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human TGF-β1 (Life Technologies) was prepared in sterile water with 0.1% BSA following the manufacturer’s instructions and diluted in 50:50 media.
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bioRxiv - Microbiology 2022Quote: ... and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). Next ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant IGF1 (Life Technologies, cat# PHG0071) and picropodophyllin (PPP ...
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bioRxiv - Genomics 2019Quote: ... 50ng/mL recombinant murine EGF (Gibco), and 100ug/mL recombinant human Noggin (Peprotech) ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant vitronectin (A14701SA, Thermo Fisher) was diluted in DPBS at the concentration of 1:200 to get the final concentration of 2.5 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant TNFα (Thermo Fisher, cat# PHC3015) was reconstituted at 0.1mg/ml in water ...
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bioRxiv - Cell Biology 2023Quote: ... mouse recombinant Egf (Life Technologies #PMG8043), 100 ng/mL mouse recombinant Noggin (Peprotech #250-38 ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant DKK1 was purchased from Gibco through Fisher (catalog number PHC9214) ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant human BMP4 (Thermo Fisher Scientific) at 10 ng/ml was added every third day in the presence of EGF and bFGF in the media ...
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bioRxiv - Microbiology 2024Quote: ... Heat stable recombinant human bFGF (Gibco) at 5U/ml was supplemented to organoid formation media only on day 0 ...
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bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
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bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Microbiology 2020Quote: ... and cultured in RPMI medium supplemented with 10% FBS and differentiated for 7 days with 20 ng/mL recombinant human Macrophage-Colony Stimulating Factor protein (M-CSF) (Thermo Fisher Scientific), and 100U/L of penicillin-streptomycin solution (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 125ng of total recombinant protein was separated on 4-20% or 10-20% Novex WedgeWell Tris-Glycine precast gels (Thermofisher, Waltham, MA). For silver stain ...
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bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with 1μg/ml of biotinylated recombinant ACE2 protein for 1 hour at room temperature followed by incubation with HRP-conjugated streptavidin (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.4 picomol of folded reporter mRNA was mixed with 20 picomol of recombinant protein and 200 picomol of UltraPure BSA (Thermo Fisher # AM2618) in binding buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 28 fmol of folded reporter mRNA was mixed with 0-10 picomol of recombinant protein and 100 picomol of UltraPure BSA (Thermo Fisher # AM2618) on ice for 1 hr ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Microbiology 2023Quote: Concentration of recombinant proteins were determined by measuring absorbance at 280 nm using a NanoDrop 1000 and/or NanoDrop One (Thermo Fisher Scientific) considering the extinction coefficient and the molecular weight calculated with the ProtParam tool (http://www.expasy.org/tools/protparam.html).
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bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...