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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... populations were subject to either “rest” (low dose 30U/mL IL-2 only) or stimulation using Dynabeads Human T-Expander CD3/28 (ThermoFisher Scientific) at 1 million cells/mL at a ratio of 2 beads per cell for 48 hours ...
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bioRxiv - Cancer Biology 2022Quote: Peptide samples were analyzed on a Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Rockford, IL, USA) coupled with a high-performance liquid chromatography system (EASY nLC 1200 ...
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bioRxiv - Immunology 2022Quote: ... and activated for 48h with 30 U/ml human IL-2 and anti-CD3-anti-CD28-beads (Dynabeads™ human T-Activator CD3/CD28, ThermoFisher). Retroviral packaging cells RD114 were seeded to reach a confluency of 60% on the day of transfection and subsequently transfected with plasmids containing the neoTCR-α- and -β-chain-sequences using TransIT®-293 (MirusBio) ...
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bioRxiv - Neuroscience 2022Quote: ... using a ViiATM7 Real Time PCR System or QuantStudio 6 FLEX Real Time PCR System (ThermoFisher Scientific Inc., Rockford, IL USA). Expression changes were calculated using the 2-ΔΔCt method normalized by Hprt as housekeeping gene ...
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bioRxiv - Physiology 2023Quote: ... Each cDNA sample was amplified with messenger RNA (mRNA) specific TaqMan Gene Expression Assays (IL-6, Mm00446190_m1; MCP-1, Mm00441242_m1; GAPDH, Mm99999915_g1; Thermo Fisher Scientific, Waltham, MA) on a CFX-96 real-time polymerase chain reaction (PCR ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants of cells maintained in normoxic condition or in 1% atmospheric oxygen in the presence of IL-1β (Thermo Fisher Scientific) for 24 hours were collected as described in (Kieran et al. ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs to be terminally differentiated were passaged on three days after IL-6 stimulation (D21) into 50ng/ml poly-l-ornithine (Gibco; A3890401) and 20ng/ml laminin (Sigma ...
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bioRxiv - Genetics 2023Quote: ... they were briefly rinsed with PST and incubated in secondary antibodies and Hoechst 33342 (1:5,000; Thermo Fisher, Rockford, IL, USA) for DNA staining two hours at room temperature.
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bioRxiv - Microbiology 2024Quote: ... The cells were pelleted (400 x g for 10 minutes) and used further for analysis of cytokine mediators IFN-γ and IL-17 (ThermoFisher) for ELISA analysis ...
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bioRxiv - Genomics 2023Quote: Cell lysates were prepared in chilled RIPA buffer (25 mM Tris-HCL pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Scientific, Rockford, IL) containing protease inhibitor (complete Mini ...
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bioRxiv - Neuroscience 2023Quote: ... Lateral striatum was dissected from each hemi-section and placed in HABG media (Hibernate A Media, BrainBits, Springfield, IL, HA500; 2% B27 Supplement, ThermoFisher, Cat #17504044; 0.26% Glutamax, ThermoFisher, Cat# 35050061). For each sample (ipsilateral and contralateral) ...
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bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...
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bioRxiv - Neuroscience 2023Quote: ... These lysates were then heated to 95°C for 10 min to denature the proteinase K (Thermo Scientific, Rockford, IL, USA), and centrifuged at 16,100 g for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used were: Alexa Fluor Plus 488 (A32723, A32790, A32731, and A32814; Thermo Fisher Scientific Inc, Rockford, IL, USA), and Alexa Fluor 488 (A11055) ...
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bioRxiv - Biochemistry 2023Quote: ... The anti-F or G mAbs were prepared under serum-free conditions using HyClone SFM4MAb (Thermo Fisher Scientific Inc., Rockford, IL) and purified by protein G-Sepharose affinity chromatography.
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bioRxiv - Genetics 2023Quote: ... The whole assay was performed in a Nunc-Immuno MicroWell 96-well clear-flat-bottom plate (Thermo Fisher Scientific, Rockford, IL). The optical density at 570 nm was measured using Cytation 3 Multi-Mode Reader (Bio-Tek Instruments ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with anti-IL-1β at RT for 1hr and samples were acquired on an Attune NxT (Life Technologies). The data were analyzed with FlowJo software (Tree Star).
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bioRxiv - Microbiology 2023Quote: ... were used as the secondary antibodies for detection and the color wasa developed using TMB substrate (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Cancer Biology 2023Quote: Peptides were resuspended in 200 mM HEPES (pH 8.5) and a 100µg aliquot of peptides was mixed with tandem mass tags (TMT, ThermoFisher Pierce, Rockford, IL) at a label to protein ratio of 2:1 ...
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bioRxiv - Neuroscience 2023Quote: ... made on the basis of DMEM/F12 with penicillin/streptomycin and supplements: 10% horse serum (Thermo Fisher Scientific, Rockford, IL, USA), 2 mM glutamine ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR reactions were carried out using Maxima SYBR Green/ROX qPCR MM (Thermo Scientific, product no. K0221, Rockford, IL, USA) in an Applied Biosystems StepOne Plus™ Real-Time PCR System (Foster City ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Immunology 2020Quote: Cytokine concentrations for IL-17A and IFNγ from the supernatants of cultured cells and ex vivo colon organ cultures were measured by ELISA (Thermo Fisher Ready-Set-Go). Samples and standards were measured in duplicate ...
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bioRxiv - Biochemistry 2021Quote: ... plates were washed 4 times with TBS-T and proteins were detected by addition of SuperSignal™ ELISA Femto substrate (ThermoFisher cat. no. PI37074). Luminescence was read using a BMG POLARstar Omega plate reader or Biotek Neo2 microplate reader.
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bioRxiv - Microbiology 2020Quote: Chimeric proteins were diluted to 1 μg/ml in carbonate buffer (pH 9.6) and used to coat polystyrene 96-well high-binding ELISA plates (100 μl/well; Nunc-Immuno plate with MaxiSorp surface). After overnight incubation at 4 °C ...
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bioRxiv - Microbiology 2020Quote: Rabbit serum immunized with DENV-1 Mochizuki strain (in-house product) was coated onto a Nunc Maxisorp ELISA plate (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Pathology 2022Quote: ... The reaction was stopped with 3N H2SO4 (50 μL/well) and read at 492 nm on a MultiSkan GO ELISA plate reader (Thermo Fisher Scientific, Vantaa, Finland) [35].
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bioRxiv - Bioengineering 2019Quote: ... conditioned media collected from cells was assessed with enzyme-linked immunosorbent assays (ELISA) in order to quantify the concentration of extracellular tumor necrosis factor alpha (TNFα, Invitrogen, cat. #88-7346-88) or Dex (Bioo Scientific ...
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bioRxiv - Immunology 2020Quote: ... were diluted in 50 mM carbonate buffer to a concentration of 1 μg/ml and coated at 50 ng/well on 96-well ELISA plates (MaxiSorp Nunc, Thermo Fisher Scientific K.K., Japan) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Specifically, 1 μg/ml purified hACE2 protein (Kactus, ACE-HM501) was coated onto a 96-well ELISA plate (Thermo Fisher Scientific, 44-2404-21) at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... The bacteria were cultured at 37°C for 48 hours and the concentrations were measured at multiple time points by the ELISA plate reader (Multiskan Go, Thermo Scientific, Waltham, MA, USA) at the OD of 600nm.
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bioRxiv - Immunology 2023Quote: Peptide competitions were undertaken by incubating RAVV GP ΔMuc at 200 ng per a well in Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Direct pepscan analysis was undertaken by binding 200 ng of each overlapping peptide to Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Genomics 2020Quote: Mouse ESCs (mESCs) were cultured in mouse ESC media that contains DMEM (Dulbecco’s modified Eagle’s medium) (Thermo Fisher Scientific) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... mouse serum samples were added to rat anti-mouse IgE mAb-coated 96-well Nunc MaxiSorb plates (Thermo Scientific). An HRP-conjugated goat anti-mouse IgE antibody (SouthernBiotech ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... A total of 21,981 mouse genes were included in the Affymetrix Mouse Clariom S Assay (Affymetrix, Santa Carla, CA) and all steps were performed at the McGill University and Génome Québec Innovation Centre (Montréal ...
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Pathology 2021Quote: ... The supernatant was moved to a clean tube and incubated overnight with 0.5 μg of either goat anti-mouse KIM-1 or mouse anti-MUC1 (B27.29) antibodies and Protein G conjugated to Sepharose 4B (ThermoFisher). Beads were washed with 0.5 ml HEPES-buffered saline (HBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... LAMP1 was stained with a mouse primary antibody (DSHB 1D4B) and anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: Mouse ESCs (mESCs) were cultured in mouse ESC media that contains DMEM (Dulbecco’s modified Eagle’s medium) (Thermo Fisher Scientific) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488 anti-mouse IgG2b, Alex Fluor 555 anti-mouse IgG2a, or Alex Fluor 647 anti-rabbit IgG; 1:1,000, Invitrogen). Slides were observed using an LSM 710 Zeiss confocal laser microscope ...
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bioRxiv - Genetics 2024Quote: ... followed by HRP-conjugated goat-anti mouse secondary antibodies at 1/20,000 dilution (Goat anti-mouse-HRP conjugate, cat# 31430, Thermofisher). The proteins were detected by SuperSignal chemiluminescent substrate (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... and a human IL-6 siRNA pool (Horizon Discovery, LQ-007993-00-0005) were transfected using Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen, 13778) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... At days 15-21 after PH induction, SRT2104 (InvivoChem, Libertyville, IL) suspended in the warm corn oil (ACROS Organics, Fair Lawn, NJ) (100mg/kg/day ...
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bioRxiv - Immunology 2020Quote: ... MIN6 cells were either exposed to pro-inflammatory cytokines (17U/mL IL-1β, 167U/mL TNFα and 17U/mL IFNγ; all cytokines were supplied by eBioscience Affymetrix, Paris, France) 254 nm UVB irradiation 10 mJ ...
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bioRxiv - Immunology 2019Quote: ... 11-7139-42 or 85BRD, cat.: 11-7136-42), IL-4-PE (8D4-8, cat.: 12-7049-42) (all from Thermo Fisher Scientific); TNFa-Pacific Blue (MAb11 ...