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Citations for Thermo Fisher :
3851 - 3900 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Sequences of full-length Dprs and DIPs with an N-terminal V5 tag were cloned into the pMT/BiP/V5-His A vector (Invitrogen) for expression in S2 Drosophila cells for PLC cleavage assay or into the pAcGP67 baculovirus transfer vector (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: The region coding for the full-length Hermes transposase (Uniprot Q25438, with the mutations Q2E and K128G) was cloned into pBAD/Myc-His (Invitrogen) in such a way that the recombinant protein was not tagged ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 1% FBS (CPS Serum, Cat. No. FBS-500-HI) and 1X penicillin/streptomycin (Gibco, Cat. No. 15140-122), followed by centrifugation in an Eppendorf® centrifuge (model 5810 R ...
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bioRxiv - Immunology 2023Quote: ... Both cell lines were cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) and 100 U/mL penicillin-streptomycin (Gibco). Cells were incubated at 37°C with 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Microbiology 2022Quote: ... Infected Vero cell monolayers were immediately overlaid with DMEM 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500). R ...
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bioRxiv - Developmental Biology 2023Quote: Peaks enriched in neural crest samples were cloned from genomic DNA using standard PCR methods using AccuPrime Hi-Fidelity Taq polymerase (Invitrogen). Primers used to amplify target enhancer sequences are provided in the Key Resources table (Table S1) ...
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bioRxiv - Biochemistry 2023Quote: ... A total of 2 mg of lysate per condition together with 25 μL of DynabeadsTM for His-Tag isolation (Invitrogen) were incubated with His6-AtLEGβ or the His6-proAtLEGβ-C211A dead mutant or lysate alone for an hour at room temperature with rotation ...
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bioRxiv - Microbiology 2023Quote: ... All host defense systems identified in the various screens were placed under the araBAD promoter (arabinose inducible) in the pBAD Myc/His A vector (Invitrogen). Conjugative allelic exchange was carried out using R6K-origin pKEK2201 (kanamycin ...
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bioRxiv - Molecular Biology 2023Quote: ... and restriction enzyme sites for NotI and EcoRV were introduced for cloning into the pcDNA4 myc/His backbone (Thermo Fisher). For NARS2-Apex2 cloning ...
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bioRxiv - Neuroscience 2023Quote: ... respectively) were amplified by PCR and inserted into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific, Waltham, MA). Fragments of genomic DNA upstream of the first methionine codon (2677 bp ...
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bioRxiv - Cell Biology 2023Quote: ... The protein samples were then separated by SDS-PAGE gels and transferred to the PVDF membrane for either immunoblotting with His antibody (Invitrogen) or staining with Ponceau S ...
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bioRxiv - Biochemistry 2024Quote: ... His-Smt3-Cysless GG-FAT10 (FAT10C0) was gene synthesized and subcloned into a pET28 vector by GeneArt (Thermo Fisher Scientific), the mutations are C7T ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted reaction was then transferred to a tube containing 80 µg of pre-washed Dynabeads His-Tag Isolation and Pulldown magnetic beads (Invitrogen) and incubated on a roller for 5 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... and DsRed were cloned into pcDNA6 vector between the XhoI and XbaI sites (pcDNA6/Myc-His B; Invitrogen, Waltham, MA) with a poly(A)-tail of 84 nucleotides (Wakai et al. ...
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bioRxiv - Cell Biology 2020Quote: ... in imaging medium (custom; DMEM High-glucose [Gibco] without Riboflavin and Phenolred containing 10% [w/w] FCS [Gibco] ...
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bioRxiv - Immunology 2020Quote: IL-2/Fc fusion proteins were expressed using the Expi293 expression system according to manufacturer instructions (Thermo Scientific). Proteins were as human IgG1 Fc fused at the N-or C-terminus to human IL-2 through a (G4S)4 linker ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FCS (Gibco™ Newborn Calf Serum [heat inactivated], of New Zealand origin; Thermo Fisher Scientific), 5% AA (Gibco® MEM) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were pre-incubated with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 µL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:50,000 ...
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bioRxiv - Bioengineering 2021Quote: The purified Fc fusion baits were captured on 96-well protein-G coated plates (Thermo Fisher Scientific 15131). The biotinylated baits were immobilized on 96-well streptavidin-coated plates (Thermo Fisher Scientific 15500) ...
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bioRxiv - Cancer Biology 2019Quote: ... the two layers were covered with liquid RPMI 1640 medium containing 10% CS-FCS (Gibco® Life Technologies). The liquid top layer with or without 100 nM T3 was changed every 3 days ...
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bioRxiv - Cancer Biology 2019Quote: ... the two layers were covered with liquid RPMI 1640 medium containing 10% CS-FCS (Gibco® Life Technologies). The liquid top layer with or without 100 nM T3 was changed every 3 days ...
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bioRxiv - Microbiology 2021Quote: ... ATCC (Cat# CRL-3216) were maintained with growth media (DMEM supplemented with 10% fetal calf serum (FCS, Invitrogen) and 100 U/ml penicillin and 0.1 mg/ml streptomycin (P/S ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μM of FGFR2-Fc (Curebio) was pre-incubated with 0 ∼ 10 μM of BSA (Thermo fisher scientific) or 1 ∼ 10 μM of AIMP1 (Curebio ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... the copy number was quantified independently by performing FCS using Alexa Fluor 568 NHS ester (Thermo Fisher Scientific) to measure the confocal volume and using the cells that transiently-express mCherry alone to convert fluorescence intensity to the concentration.
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody (AlexaFluor488 donkey anti mouse IgM 1/400, Life Technologies in Buffer A, 0.1% Saponin 2% FCS) was performed ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were pre-incubated with mouse-Fc block and viability marker prepared in calcium/magnesium-free PBS (Gibco), before staining with fluorescently-conjugated antibodies prepared in staining buffer (2mM EDTA ...
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bioRxiv - Cancer Biology 2020Quote: Cells were maintained at 37 °C under humidified 5% CO2 in DMEM medium supplemented with 10% FCS (Invitrogen) and 2 mM glutamine ...
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bioRxiv - Microbiology 2020Quote: ... spun down at 1,000 × g for 10 min and pooled in equal proportions in Grace’s medium (Grace’s insect media [Sigma-Aldrich] with 4.2 mM NaHCO3, 10% FCS [Gibco], 1 × Penicillin/Streptomycin [Gibco] and 1 × BME Vitamins [Sigma-Aldrich] and adjusted to pH 5.25 ...
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bioRxiv - Microbiology 2020Quote: ... spun down at 1,000 × g for 10 min and pooled in equal proportions in Grace’s medium (Grace’s insect media [Sigma-Aldrich] with 4.2 mM NaHCO3, 10% FCS [Gibco] ...
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bioRxiv - Immunology 2019Quote: ... Antibody staining was performed in PBS + 1% FCS for 30min at 4°C for live-dead marker (Invitrogen), α-CD8 (RPA-T8 ...
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bioRxiv - Immunology 2019Quote: RMA and RMA/s thymoma cells (cultured in Iscove’s modified Dulbecco’s medium supplemented with 10% FCS; Life Technologies) were labeled with Cell Trace Blue or Cell Trace Violet ...
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bioRxiv - Cell Biology 2020Quote: ... and the interface containing stromal cells was washed in DMEM/F12/ 10% fetal calf serum (FCS, Invitrogen, USA) and 1% primocin (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... Absorbance was measured at 450 nm with Multiskan™ FC Filterbased Microplate Photometer (Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Zoology 2021Quote: ... Cells were then resuspended in L-15 (Leibovitz) medium containing 10% heat-inactivated fetal calf serum (FCS, Gibco) and antibiotics (penicillin–streptomycin ...
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bioRxiv - Cell Biology 2020Quote: MDA-MB231 and HeLa cells were cultured in 4.5 g/L glucose DMEM supplemented with 10% FCS (Gibco), 10 U/mL penicillin (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated hACE2-Fc was prepared using EZ-Link™ Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Biosera – FB 1001/500) and 2 mM GlutaMAX™ (Gibco – 35050061) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... to each well and the plates read at 450 nm using a Multiscan FC Plate Reader (Thermo Scientific). ELISA results were presented as normalised OD450 with the mean OD450 values of blank wells subtracted from the sample well values.
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bioRxiv - Immunology 2020Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Caco-2WT and Caco-2ΔHk2 cells were cultured in MEM with 20% (v/v) FCS purchased from Gibco/Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Biosera – FB 1001/500) and 2 mM GlutaMAX™ (Gibco – 35050061) at 37°C with 5% CO2.
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bioRxiv - Immunology 2021Quote: ... The optical density was measured at 450nm (OD450) using an accuSkan FC microwell plate reader (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... and lymphocytes were resuspended in complete RPMI (cRPMI- RPMI + 10% FCS + 2% Penicillin-Streptomycin + 1% L-glutamine (Gibco)) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was used to measure the m6A level in total RNA by MultiSkan FC microplate reader (Thermo Fisher, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies ...
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bioRxiv - Physiology 2024Quote: ... The isolated cells were plated (0.1 % gelatine-coated T75 flasks, approx. 1.0×107 cells) and cultured in 10 % FCS-containing DMEM (Gibco). Medium was changed every 3 days ...
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bioRxiv - Microbiology 2023Quote: Cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...