Labshake search
Citations for Thermo Fisher :
3851 - 3900 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Recombinant lentivirus used to infect SW620 cells for protein expression was generated by the ViraPower Lentiviral Expression System (Invitrogen) according to the manufacturer’s instruction.
-
bioRxiv - Immunology 2024Quote: ... GPI-RBD-hGM-CSF recombinant fusion protein and GPI-hIL-12 were affinity purified from CHO-S cells (Invitrogen) stably expressing the proteins using anti-human GM-CSF (clone BVD2-21C11 ...
-
bioRxiv - Microbiology 2024Quote: ... the sequence of the resulting recombinant plasmid was ascertained by Sanger sequencing techniques (Applied Biosystems™ 3130 Genetic Analyser), and the resulting data were decoded by ApE software (v2.0.36 ...
-
bioRxiv - Biochemistry 2020Quote: ... Cytosolic extracts were generated as described above and the His6-tagged proteins purified by metal affinity chromatography and gravity flow using 0.5 ml HisPur™ cobalt superflow agarose (Thermo Fisher Scientific™, Waltham, USA) according to manufacturer’s protocol ...
-
bioRxiv - Biophysics 2019Quote: ... The purity of affinity purified histidine tagged CsgF was investigated using SDS PAGE and the presence of the histidine tag was confirmed by western blot using an Anti-His (C-Term)-HRP antibody (Life Technologies, Carlsbad, CA).
-
bioRxiv - Molecular Biology 2021Quote: ... enrichment and chip loading was performed on an Ion Proton Chef system using the Ion PI™ Hi-Q™ Chef Kits (ThermoFisher Scientific); sequencing was performed on an Ion Proton PI™ V3 chip ...
-
bioRxiv - Immunology 2022Quote: ... Human PBMCs and C57BL/6 mouse PBMCs not immediately used in any experiments were stored down by resuspending cells in freezing media (HI-FBS with 10% DMSO, Fisher Scientific, BP231-100) in aliquots of 5-10 x106 cells per ml and frozen down to -80°C at a speed of 1°C/min prior to storage in liquid nitrogen.
-
bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed by using qPCRBIO Syber Green Mix Hi-ROX (PCR Biosystems, Wayne, US) and run with the StepOne Real-Time PCR System (Applied Biosystems, Waltham, US). The relative gene expression was calculated as 1/2ΔCT based on the normalization by the reference gene Hprt1 ...
-
bioRxiv - Cell Biology 2020Quote: ... purified His-calmodulin was dialyzed in a Spectra/Por™ 4 RC Dialysis Membrane Tubing (08-667D, Thermo Fisher Scientific, Waltham, MA, USA) against 1,000 volumes of PBS supplemented with 0.5 mM EGTA at 4 °C for 4 h and replaced with fresh 1,000 volumes of PBS twice.
-
bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
-
bioRxiv - Molecular Biology 2024Quote: ... were amplified from differentiated P19 mouse embryonal carcinoma cells15 and ligated into the multiple cloning site of the expression vector pcDNA3.1 myc-His B (Thermo Fisher Scientific; Waltham, MA). Snapin was cloned in-frame with the downstream myc-His tags ...
-
bioRxiv - Genetics 2024Quote: ... Membrane was stripped using PVDF stripping solution (DOT Scientific 65000-500) according to manufacturer instructions and reprobed with anti-6x-His primary antibody (Thermo Fisher MA1-21315) and conducted identically to the first probing ...
-
bioRxiv - Neuroscience 2023Quote: An PPP2R2B-AS1 fragment containing the region 44 bp upstream of the CTG repeat and 145 bp downstream from the repeat was PCR-amplified and cloned into pcDNA3.1(-) myc-his A vector (Life Technologies, Grand Island, NY) at EcoRV site ...
-
bioRxiv - Immunology 2023Quote: ... The resulting libraries were multiplexed and sequenced with Ion Proton technology and Ion Hi-Q chemistry (Ion Torrent, Thermo Fisher Scientific, Vienna, Austria). CUTADAPT 4.0 was used to trim sequencing reads of low-quality bases and poly-A tails with the options -q 20 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and the pull-down assay follows the protocol provided by Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, Waltham, MA). The fluorescence intensity (excitation = 485 ...
-
bioRxiv - Biochemistry 2023Quote: ... The membrane was probed with primary antibodies including anti-tau mouse ET3 (1:1000 dilution) and 6X - His Tag rabbit monoclonal antibody (Invitrogen, 1:3500 dilution). The secondary antibodies used were ...
-
bioRxiv - Cell Biology 2023Quote: Primary antibodies used in western blotting (WB) and immunofluorescence (IF) were anti-6x His mouse monoclonal (Invitrogen, #MA1-21315, 1:5000 in WB), anti-GST mouse monoclonal (Invitrogen ...
-
bioRxiv - Plant Biology 2024Quote: ... to digest the phosphorylated OsPrx20-His and analyzed by the LC-MS/MS system (LTQ Orbitrap XL ETD; ThermoFisher Scientific, Waltham, MA, USA) as described previously (Gampala et al. ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
-
bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
-
bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
-
bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2020Quote: Recombinant expressions of proteins involved in this study were performed by using the ExpiCHO™ expression system (Thermo Scientific, A29133). Briefly ...
-
bioRxiv - Microbiology 2019Quote: ... Sequences of recombinant RVAs were determined from cDNAs prepared from viral RNAs using a Superscript III reverse transcriptase kit (Invitrogen).
-
bioRxiv - Biochemistry 2022Quote: ... The drug-selected recombinant CHO-S cells were adapted to serum-free suspension culture using CHO-S medium (CD Forti CHO; ThermoFisher). For large-scale cultures ...
-
bioRxiv - Neuroscience 2022Quote: ... After centrifugation, nuclei pellets were resuspended in 1 ml PBS-RI (PBS, 50 U/mL Rnase-OUT Recombinant Ribonuclease Inhibitor (Invitrogen), 1 mM DTT ...
-
bioRxiv - Cell Biology 2022Quote: ... the LRRK1RCKW plasmid was used for the generation of recombinant baculoviruses according to bac-to-bac expression system protocols (Invitrogen).
-
bioRxiv - Biochemistry 2020Quote: ... The resulting plasmid was utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen). Exponentially growing Sf9 cells (2E06 cells/mL in Lonza Insect-XPRESS medium ...
-
bioRxiv - Immunology 2021Quote: ... Individual B cells were sorted into 96-wells PCR plates (Bioké) containing 2 μL lysis buffer (0.2% (v/v) Triton X-100 + 2U/μL RNaseOUT™ Recombinant Ribonuclease (Invitrogen) in UltraPure™ DNAse/RNase free distilled water (Invitrogen) ...
-
bioRxiv - Plant Biology 2020Quote: ... Purified recombinant HisHA-INF1 or HisHA-Scr74 were incubated with 20 μl Pierce Anti-HA magnetic beads (Thermo Fisher Scientific) at an amount of 500 pmol each for 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... 5 × 106 HEK 293T cells were co-transfected with 6 μg each of pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 S plasmids (pS-D614 or pS-G614) using Lipofectamine 3000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... crude extracts and purified recombinant SepF and FtsZ in serial dilution were loaded on a 4−12 % (w/v) BisTris PAGE gel (Invitrogen). The amounts of crude extract were 120 µg for FtsZ and 290 µg for SepF ...
-
bioRxiv - Plant Biology 2020Quote: ... The cDNAs of NPH3SA and NPH3SE within the pENTR vectors were transferred to both pH35GS and pH35YG binary vectors (Yamaguchi et al., 2008) using an LR recombinant reaction (Invitrogen). 35Spro:NPH3SA ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) or CaptureSelect™ IgA Affinity Matrix (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... 59°C for 30s and 72°C for 30s using recombinant 5U/ul Taq polymerase prepared in 10X Buffer with 50mM MgCl2 (Invitrogen). The 388bp PCR amplicon was digested with 20U/µL EcoRV restriction enzyme prepared in 10X NEB 3.1 buffer (New England Biolabs ...
-
bioRxiv - Immunology 2022Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
-
bioRxiv - Cell Biology 2022Quote: Ribonucleoprotein complexes containing 3.9 picomoles of ATF3 sgRNA guides and 3 picomoles of purified recombinant Cas9 protein (Synthego) were transfected into HEK293T cells using Lipofectamine CRISPRmax (Invitrogen). 72 hours post transfection ...
-
bioRxiv - Immunology 2022Quote: NK cells were enriched and incubated with 50 ng/ml recombinant mouse IL-15 (Preprotech) for four days in RPMI plus 10 % FBS with penicillin and streptomycin (Thermofisher) added in a U-bottom 96-well plate at the concentration of two million cells per ml ...
-
bioRxiv - Immunology 2021Quote: ... The recombinant plasmids were transfected into Drosophila S2 cells using Calcium Phosphate Transfection Kit (Thermo Fisher Scientific, Paisley, Scotland, UK). Following antibiotic selection with Zeocin (InvivoGen ...
-
bioRxiv - Microbiology 2020Quote: ... a recombinant bacmid DNA was generated from the sequencing-confirmed pFastBac construct using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
-
bioRxiv - Microbiology 2021Quote: ... The bacmid was transfected into Sf9 cells to obtain recombinant baculovirus using Cellfectin II Reagent (Invitrogen Co., Carlsbad, CA, USA) according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2020Quote: ... fluorescent conjugate cholera toxin B (CTB; recombinant cholera toxin subunit B conjugated with Alexa fluorophores: 0.2% CTB-488 and CTB-594; Life Technologies) was injected into AL and PM.
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were sonicated and recombinant proteins purified in a single step on Ni-agarose column according to manufacturer instructions (Invitrogen). Protein samples were dialyzed against PBS with 5 mM MgCl2 and 0.1% glycerol for 2h and used immediately in experiments ...
-
bioRxiv - Microbiology 2020Quote: ... the macrophages were allowed to adhere to the culture plate for 20 minutes before 100 units (U) of murine recombinant interferon-gamma (IFNγ) (Invitrogen) was added to each well to stimulate the macrophages ...
-
bioRxiv - Immunology 2020Quote: ... A total of 100.000 T cells were co-cultured with 50,000 sorted myeloid cells in the presence of 30IU recombinant hIL-2 plus anti-CD3/CD28 Dynabeads (ThermoFisher Scientific) for 3-4 days in round-bottom plates ...
-
bioRxiv - Bioengineering 2021Quote: Recombinant coronavirus spike glycoproteins S (Table S1) were coated overnight onto NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) at 50 ng/well in 100 µL of PBS ...