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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and AlexaFluor594 goat anti-mouse (Invitrogen, A11032).
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bioRxiv - Cell Biology 2024Quote: ... AFSSS goat anti-mouse (A28l80, Invitrogen, l:l000) or AFS94 goat anti-mouse (All00S ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa-488 goat anti-mouse IgG (ThermoFisher), then with DAPI ...
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bioRxiv - Developmental Biology 2024Quote: ... and Mouse anti-Biotin (Invitrogen 03–3700) were used (1:400) ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibodies (mouse anti-GFP; Molecular Probes, Cat # A11120 ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mg/ml mouse laminin (Invitrogen 23017015), and 2 μg/ml doxycycline.
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bioRxiv - Neuroscience 2024Quote: ... 1 mg/ml mouse laminin (Invitrogen, 23017015), and 2 μg/ml doxycycline) ...
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bioRxiv - Neuroscience 2024Quote: ... mouse CYP7B1 (OTI1G7) 1:500 (TA807549, Thermofisher), rabbit HSD3B7 1:1000 (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-mouse (Invitrogen, Catalog # 31430,1:5000) antibodies in blocking buffer for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-Mouse IgG HRP-conjugated (ThermoFisher), goat anti-Mouse IgG Alexa Fluor 488-conjugated (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... The sections were subsequently incubated with goat anti-rabbit Alexa Fluor 488 (1:200, #11034, Thermo Fisher Scientific, Rockford, IL, USA) at 37 °C for 2 h Images were visualized using a confocal laser scanning microscope (ZEISS ...
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bioRxiv - Immunology 2021Quote: ... and wild type or IL-7rα-/- (both CD45.2+) were depleted of lineage-positive cells by magnetic separation using Biotin Binder Dynabeads (Thermo Fisher Scientific) after staining with biotinylated antibodies against CD3 ...
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bioRxiv - Evolutionary Biology 2021Quote: YFP-tagged C- and N-terminal in-frame fusions of human SLC30A9 sequence (NP_006336.3) were synthesized by Vectorbuilder (Chicago, IL, USA) and transfected in HeLa cells using Lipofectamine 3000 (ThermoFisher, Waltham, MA, USA). Before transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... pregnant dams received intraperitoneal EdU injections of 30 μg/g body weight 2 hours before sacrificing (Thermofisher/Invitrogen, Rockford, IL; #E10187).
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bioRxiv - Developmental Biology 2020Quote: ... pregnant dams received intraperitoneal EdU injections of 30 μg/g body weight 2 hours before sacrificing (Thermofisher/Invitrogen, Rockford, IL; #E10187).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SuperSignal West Pico PLUS Chemiluminescent Substrate (Pierce, Rockford, IL) (or, for CD47, SuperSignal West Femto Maximum Sensitivity) was used for detection on an iBright FL1000 (Thermo Fisher) in chemiluminescence mode.
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bioRxiv - Molecular Biology 2021Quote: ... The cells were stained with secondary antibodies labeled with Alexa-Fluor 488 and Alexa-Fluor 555 (ThermoFisher Scientific, Rockford, IL, USA). Cells were incubated with nuclear-DAPI staining ...
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bioRxiv - Immunology 2021Quote: ... 100 units penicillin and 100 μg streptomycin per ml) supplemented with 50 U/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads/ml (Life Technologies). The next day ...
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bioRxiv - Immunology 2021Quote: ... and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final: 10% FBS, 1x GlutaMAX™, 50µM 2-mercaptoethanol Thermo Scientific™ ...
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bioRxiv - Immunology 2019Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely recon-stituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Molecular Biology 2019Quote: ... Then the peptides were separated with high-performance liquid chromatography (HPLC, Ultimate 3000 Nano LC System, Thermo Scientific, Rockwell, IL, USA) in a 15-cm long C18 column (Acclaim® PepMap™ RSLC inner diameter of 75 μm ...
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bioRxiv - Neuroscience 2020Quote: RNA extraction was performed using whole hippocampi.34 RNA concentration was measured via a nanodrop Spectrophotometer (Thermo Scientific, Rockford, IL, U.S.A) and absorbance determined at a ratio of 260/280 ...
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bioRxiv - Microbiology 2021Quote: ... The beads were washed three times with cold lysis buffer on Pierce™ Spin Columns - Screw Cap (Thermo Scientific, Rockford, IL) and then suspended in 15 µl 1x kinase buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Then the peptides were separated with high-performance liquid chromatography (HPLC, Ultimate 3000 Nano LC System, Thermo Scientific, Rockwell, IL, USA) in a 15-cm long C18 column (Acclaim® PepMap™ RSLC ...
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bioRxiv - Microbiology 2021Quote: ... RNA polymerase subunit β was used as a loading control and detected using anti-RNAP antibody (Thermo fisher, Rockford, IL, USA). Densitometry was performed using the ImageJ software (71 ...
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bioRxiv - Microbiology 2021Quote: ... Activation of CD4+ T cells was achieved by addition of 25 U/ml of interleukin-2 (IL-2) and CD4+ T-cell activation Dynabeads (Life Technologies). CD4+ T-cells activated for 4-5 days were used for HIV-1 infections ...
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bioRxiv - Microbiology 2020Quote: ... The membranes that were sequentially probed with more than one antibody were stripped in stripping buffer (Thermo Scientific, Rockford, IL, USA) before re-probing.
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bioRxiv - Cancer Biology 2021Quote: ... a drop of prolong gold anti-fade reagent with DAPi was added to each section (Thermo Fisher Scientific, Rockford, IL, USA), and slides were mounted and sealed with nail polish ...
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bioRxiv - Cancer Biology 2021Quote: ... A drop of prolong gold anti-fade reagent with DAPi was added to each section (Thermo Fisher Scientific, Rockford, IL, USA), and slides were mounted and sealed with nail polish ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble supernatant was collected and protein concentration was quantified via bicinchoninic assay (BCA) assay (Pierce Thermo Scientific, Rockford, IL, USA). Normalized soluble supernatant or 20 μL of conditioned media was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Neuroscience 2020Quote: ... These lysates were then heated to 94°C for 10 minutes to denature the proteinase K (Thermo Scientific, Rockford, IL, USA), and centrifuged at 16,100g for 15 minutes ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was collected and protein concentration was quantified via a bicinchoninic assay (BCA) assay (Pierce Thermo Scientific, Rockford, IL, USA). Approximately 30 μg of soluble supernatant was run on a 4-20% Tris-Gly SDS PAGE gel (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Molecular Biology 2022Quote: ... and supplemented with 100 U/mL of penicillin G and 100 μg/mL of streptomycin (SV30010; Thermo Scientific, Rockford, IL, USA) at 37 °C in a humid atmosphere of 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... and (2) co-cultured with KPC CAFs with or without concurrent treatment with anti-IL-1β neutralizing antibody (Thermofisher, Waltham, MA) and IL-1R1 inhibitor Anakinra (SOBI Pharmaceuticals ...
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bioRxiv - Cancer Biology 2022Quote: ... whole-cell protein extracts from harvested splenocytes were prepared using RIPA buffer (ThermoScientific, Rockford, IL) containing a protease/phosphatase inhibitor cocktail (Thermo Scientific). Protein quantification was performed using the Pierce BCA protein assay kit (ThermoScientific ...
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bioRxiv - Immunology 2019Quote: ... 100 μg/mL streptomycin with either 110 U/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) (Leukine; Sanofi) and 282 U/mL interleukin-4 (IL-4) (Affymetrix, eBioscience) for DCs or 280 U/mL GM-CSF for macrophages ...
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bioRxiv - Synthetic Biology 2020Quote: ... Dialysis was performed using Slide-A-Lyzer™ Dialysis Cassettes with a 3.5 kDa cut-off (Thermo Scientific, Rockford, IL, USA). Extracts were dialyzed three times with 150 mL S30 buffer per mL extract for 45 min at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... ELISAs were conducted following the manufacturers’ protocols, with the IL-1b and IFN-y (ELISAKIT.com, EK-0033 and EK-0002, respectively) and TNF-a (ThermoFisher Scientific KMC4022). Samples were diluted 1 in 5 and 1 in 10 for IL-1b ...
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bioRxiv - Biochemistry 2021Quote: TE proteins were fluorescently labeled with the amine-reactive dye Alexa Fluor 647 NHS-Ester (Thermo Fisher Scientific, Rockford, IL, USA) as follows ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated in PBS plus 0.5% Triton X-100 and Alexa Fluor 568-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely reconstituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Immunology 2020Quote: ... by negative selection magnetic bead sorting (Miltenyi) and activated in complete RPMI supplemented with 30 U/ml IL-2 with anti-human CD3/CD28 dynabeads (Thermo Fisher) at a 1:1 cell-to-bead ratio.
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bioRxiv - Developmental Biology 2021Quote: ... whole mount duck and quail embryos were stained with Hoechst 33342 Dye (62249, Lot# RF22228410, ThermoFisher Scientific, Hanover Park, IL, USA). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then stored at -140 °C in liquid nitrogen (CryoPlus Storage Systems model 7402, ThermoFisher Scientific, Hanover Park, IL, USA).
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bioRxiv - Cell Biology 2020Quote: ... The concentration of protein crude extract was determined by means of bicinchoninic acid (BCA) protein assay (Thermofisher Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2021Quote: Purified CD8+ cells were cultured in AIMV medium supplemented with 10% FBS and 100 U/ml recombinant human IL-2 (ThermoFisher Scientific) and used for sorting of CD8+ T cell subsets and for further experiments three days after isolation.