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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... 6’-diamidino-2-phenylindole (DAPI; Life Technologies) diluted 1/10,000 for nuclei staining ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (ThermoFisher, #D1306) at a concentration of 10 µg/ml in PBS / 3.0%BSA for 15 minutes then rinsed 3x 5 minutes in distilled water ...
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bioRxiv - Immunology 2023Quote: ... with a QuantStudio 6 Pro (Applied Biosystems). For murine Slc2a5 ...
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bioRxiv - Bioengineering 2023Quote: ... and a QuantStudio™ 6 instrument (ThermoFisher). Samples were activated at 94°C for 3 min ...
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bioRxiv - Developmental Biology 2023Quote: ... and 6 U/mL Dispase (Gibco, 17105041) for 1 hr at 37°C with constant agitation ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... using a QuantStudio 6 (Thermo Fisher Scientific). Relative LepR mRNA expression levels were determined using the 2−ΔΔCT method normalized to β-Actin mRNA expression levels ...
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bioRxiv - Cell Biology 2023Quote: ... puromycin (6 μg/ml; Thermo Fisher Scientific) was added to the select transduced cells ...
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bioRxiv - Genetics 2022Quote: ... or the QuantStudio 6 Flex (Thermo Fisher). The Ct values were analyzed by the relative fraction of input method.
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bioRxiv - Genetics 2022Quote: ... or the QuantStudio 6 Flex (Thermo Fisher). Samples were analyzed using a two-step amplification and melt curves were obtained after 40 cycles ...
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bioRxiv - Developmental Biology 2023Quote: ... using QuantStudio 6 Flex system (Applied Biosystems). Gapdh was used as endogenous control and data analyzed with ΔΔCt method ...
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bioRxiv - Cell Biology 2023Quote: ... and a 6-well plate (ThermoFisher Scientific) were brought into a biosafety cabinet using aseptic techniques ...
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bioRxiv - Immunology 2023Quote: ... 6 U/ml DNase I (ThermoFisher Scientific) and 60 U/ml hyaluronidase (Millipore Sigma ...
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bioRxiv - Immunology 2023Quote: ... no UNG (Life Technologies, Quant Studio 6). Reactions were carried out for 40 cycles ...
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bioRxiv - Genomics 2023Quote: ... using a QuantStudio 6 FLEX machine (Thermofisher). See Supplementary Data for primers ...
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bioRxiv - Neuroscience 2023Quote: ... in 6-well plates (Thermo Fisher Scientific). Each line was passaged with 0.5 mM EDTA (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... on a QuantStudio 6 Pro (Applied Biosystems). All data were normalized by 18S rRNA transcript and calculated using the ΔΔCq method ...
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bioRxiv - Biochemistry 2024Quote: ... or 6 µM blasticidin-S (Invitrogen, R21001), respectively ...
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bioRxiv - Microbiology 2024Quote: ... 6’-diamidino-2-phenylindole (DAPI; Life Technologies) diluted 1:10000 in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... 6 μl Lipofectamine 2000 (Life Technologies, # 11668) were mixed with 0.15 ml Opti-MEM ...
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bioRxiv - Neuroscience 2024Quote: ... IL-6 (cat#: 200-06; Fisher Scientific). All ligands were used at a concentration of 100 ng/mL.
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bioRxiv - Plant Biology 2024Quote: ... on a QuantStudio 6 Flex (Applied Biosystems).
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bioRxiv - Microbiology 2024Quote: ... Novex TBE 6 or 10% gels (Invitrogen) were pre-run in 0.5x TBE running buffer (pH ∼8.4 +/-0.5) ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then incubated in the NGS for at least 4 hrs and incubated overnight at 4°C with secondary antibodies: goat anti-rabbit Alexa Fluor 488 (Invitrogen Cat# A11070; 1/400) and goat anti-mouse HRP-conjugated (Thermo Fisher Scientific Cat# G-21040 ...
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bioRxiv - Bioengineering 2023Quote: ... in 2.5 μl NuPAGE® LDS Sample Buffer (4×) and 1 μl NuPAGE® Sample Reducing Agent (10×)] were loaded in Bolt 4-12% Bis-Tris Plus Gels (Thermo Fisher Scientific, USA). Samples were denatured at 95°C for 3 mins ...
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bioRxiv - Molecular Biology 2023Quote: ... and brain tissues were weighed and homogenized at a density of 140 mg of dry tissue per milliliter in ethyl acetate / isopropanol (4:1) using a Bead Mill 4 homogenizer (Thermo Fisher Scientific, Waltham MA) and 2-mL pre-filled polypropylene microtubes (2.8 mm ceramic beads ...
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bioRxiv - Cancer Biology 2022Quote: ... PANC-1 and HEK293T cells were maintained in DMEM with 4.5 g/l D-Glucose and GlutaMAX (Gibco), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 1% BSA in PBS with primary mouse anti-HuC/D antibody (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Spinal cords of P3 mice were dissected under a stereomicroscope in a solution of D-glucose 6.5 mg·ml−1 in DPBS (#14190-094, Gibco), sliced (300 μm thickness ...
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bioRxiv - Cancer Biology 2023Quote: ... EGFP-labeled MDA-MB-231 cells were cultured in low glucose DMEM (1 g/ml D-Glucose, Gibco) with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were permeabilized by incubation in 1% Triton X-100 (Cat.No. T8787, SigmaAldrich) in D-PBS +/+ (Cat.No. 14040-091, Gibco) for 15 min at RT ...
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bioRxiv - Biophysics 2023Quote: ... 200 μL of the virus dilution containing 1µg-3µg p24 antigen was transferred to a poly-D-lysine coated glass coverslip (#1 chambered coverglass, Nunc™ Lab-Tek™ ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were checked on 1% agarose gel and directionally cloned using pENTR/D-TOPO Cloning Kit (Invitrogen) and then transformed into 5-alpha competent Escherichia coli cells (NEB) ...
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bioRxiv - Immunology 2023Quote: ... After the indicated time cells were lysed in 1% n-Dodecyl-β-D-Maltoside (DDM, Thermo Scientific #89903) in lysis buffer (30 mM Tris pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... hippocampi were dissected from 1-to 2-d-old mouse neonates in cold Hank’s buffer solution (HBSS) (Gibco) supplemented with 0.08% D-glucose (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... hippocampi were dissected from 1 to 2-d-old mouse neonates in cold Hank’s buffer solution (HBSS) (Gibco), supplemented with 0.08% D-glucose (Sigma) ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM PMSF) and precleared for 1 hour at 4°C with 25 μl of protein G Dynabeads (Invitrogen). The precleared chromatin solution (1.5 × 106 cells ...
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Developmental Biology 2019Quote: ... prior to overnight incubation at 4°C in mouse anti-ZO-1 (Life Technologies, Carlsbad, CA; 1A12; 1:100). Following further buffer washes ...
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bioRxiv - Cancer Biology 2020Quote: ... new organoids were fixed with 4% paraformaldehyde for 1 day and then incubated with the phalloidin (1:40; Invitrogen) and Hoechst 33342 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Primary staining’s were performed overnight at 4 °C with antibodies to TRF2 and POT-1 (1:300; Abcam and ThermoFisher,); CD3 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... p21) and pAAV target plasmid in a 4:1:1:2 molar ratio by use of Lipofectamine 2000 (ThermoFisher) according to company protocol ...
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bioRxiv - Genomics 2024Quote: ... was coupled for 4 hours to 40µl of 1:1 mix Protein G and Protein A Dynabeads (Thermo Scientific) and used for overnight chromatin immunoprecipitation in IP buffer (1% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... The relative expression of target cDNAs within the sample set was calculated from a serial dilution (1:4–1:256) (standard curve) of a cDNA pool using StepOneTM software V2.0.2 (Applied Biosystems). Real-time PCR data were then normalized to elongation factor-1 alpha (eF1α ...
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bioRxiv - Bioengineering 2024Quote: ... Plasma samples were thawed from -80°C storage to room temperature and diluted 1:4 with 1× PBS (Gibco). Samples were then transferred to a 96-well clear bottom black plate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged with 80-90% confluence at a split ratio of 1:2-1:4 using TrypLE (Gibco) for 8 min at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... A 1 mL aliquot of 2:2:1 (v/v) mixture of ≥ 99.9% purity acetonitrile (Fisher Scientific; Cat.A998-4) ≥ 99.8% purity methanol (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The following primary antibodies and dilutions were used overnight at 4 °C in PBST with 1% milk: mouse anti- V5 conjugated to horseradish peroxidase (HRP; 1:5,000; Invitrogen), mouse anti-V5 (1:5,000 ...
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bioRxiv - Microbiology 2023Quote: ... 5′-labelled Aar RNA (4 nM) was incubated for 1 h at 37°C with 1× structure buffer (Ambion), 1 µg yeast RNA and putative mRNA interaction partners at the indicated concentrations (0 nM ...
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bioRxiv - Cell Biology 2023Quote: ... This was followed by overnight incubation at 4°C with 1:200 anti-LAMP-1 (D401sm #15665, Life Technologies). The next day ...
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bioRxiv - Immunology 2019Quote: ... 5’-CCCTACTGTATCCTCATG-3’/5’-CTTACCTCCTCTTCAATAGC-3’ PRKDC: 5’-GGGGCATTTCCGGGTCCGGG-3’/5’-TGCCCTGCCCCCCACTCTGC-3’ Amplicons were cloned using the Zero Blunt TOPO PCR Cloning kit (ThermoFisher), prepared as plasmids ...