Labshake search
Citations for Thermo Fisher :
3351 - 3400 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... Genes encoding pep86 (trademarked as ‘SmBiT’ (Dixon et al., 2015)) -tagged mitochondrial PCP proteins (from MGW Eurofins or Thermo Fisher Scientific) were cloned into either pBAD ...
-
bioRxiv - Microbiology 2021Quote: Tagged diguanylate cyclases were generated by PCR amplification of each diguanylate cyclase followed by cloning into pENTR D-TOPO (ThermoFisher Scientific) and then transferred to either pRH016 (HA tag ...
-
bioRxiv - Molecular Biology 2020Quote: ... Tagged amplicon pools were quantified using the Qubit™ 3.0 fluorometer and a Qubit™ dsDNA HS Assay Kit (Invitrogen, UK) and pooled with equimolar concentrations into a unique library ...
-
bioRxiv - Molecular Biology 2020Quote: ... mESCs were transfected with 10ug pCAG vectors encoding 2xFlag-HA-tagged BAP1 wild-type or BAP1 C91S using Lipofectamine 2000 (ThermoFisher Scientific), according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: GST pull-downs were performed by incubating purified GST tagged proteins (5 µg) with 20 μl dry volume of glutathione agarose 4B beads (Thermo Scientific) for 1 h under rotation at 4 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were grown on coverslips overnight and transfected with an mGFP-tagged donor construct (GFP-SHANK3 SPN WT or GFP SHANK3 SPN R12E/K22D) and mCherry-tagged acceptor construct (mCherry-KRASG12V) using Lipofectamine® 3000 (Invitrogen) or jet PRIME (Polyplus ...
-
bioRxiv - Cancer Biology 2022Quote: ... MIA PaCa-2 cells were grown on coverslips overnight and transfected with GFP-tagged SPN WT or SPN R12E/K22D using Lipofectamine® 3000 (Invitrogen) for 24 h as described above ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were incubated at RT for 1 hr with an anti-mouse secondary antibody tagged with AlexaFluor488 (Thermo Fisher Scientific, A11001) used at 2 μg/mL in blocking buffer ...
-
bioRxiv - Genomics 2022Quote: ... The human urothelial bladder tumor cells were transfected with the doxycycline-inducible GFP-tagged APOBEC3G vector and the PiggyBac transposase vectors with the Lipofectamine2000 transfection agent (ThermoFisher Scientific). G418 was used for the selection of the clones with stable genomic insertion of the GFP-tagged APOBEC3G component ...
-
bioRxiv - Systems Biology 2022Quote: ... Individual wells were transfected with 25ng of plasmid expressing one tagged Parkin variant using a Lipofectamine 3000 kit (Invitrogen, L3000-015) via manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... HEK 293T cells were transiently transfected with 5 µg of FLAG tagged full-length HRI construct and 5 µg of GFP tagged DELE1 constructs (DELE1CTD or mutants) using Lipofectamine 3000 transfection reagent (L3000008, ThermoFisher Scientific). GFP empty vector was used as a negative control ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no. A-11081, Thermo Fisher Scientific, USA). The detailed immunostaining protocol is available upon request.
-
bioRxiv - Microbiology 2024Quote: ... Western blotting and immunodetection of HA-tagged proteins with anti-HA and horse radish peroxidase-coupled secondary antibodies using an iBright™ FL1500 (ThermoFisher). The remaining 25 µl bead suspension was used as input for on-bead digestion (Klaus et al. ...
-
bioRxiv - Biochemistry 2023Quote: ... 24 hours later cells were transfected with 2 ug DNA (mCherry tagged PPP1R15A DNA and empty carrier DNA at a ratio of 1:19) using the lipofectamine LTX system (Life Technologies) at a ratio of 3 μl lipofectamine LTX to 1 μl Plus reagent for every nanogram of DNA in 200 μl of Opti-MEM (Thermo Fisher Scientific) ...
-
Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins (5 µg) along with GST alone were incubated with 10 µl of magnetic glutathione beads (Thermo Fisher Scientific) for one hour at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... H10,14,243F GPR65 or B2AR N-terminally tagged with FLAG were cultured in DMEM high glucose (Cytiva, SH3024301) supplemented with 10% fetal bovine serum (FBS; Gibco, 26140079). Stable cell lines expressing one of the constructs were generated using Geneticin (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... HA-tagged CDPK1 in the cWT and cMut lines was visualized with secondary goat antibodies (1:2000, 1 hr; Life Technologies) conjugated to Alexa Fluor 594 and 488 ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice with filtered PBS and then incubated with AlexaFluor 555-tagged donkey-anti-rabbit (Molecular Probes, 1:1000) and AlexaFluor 488-tagged donkey-anti-mouse (Molecular Probes ...
-
bioRxiv - Biophysics 2022Quote: ... incubated for 5 min with 25 pM (single-label) or 5 pM (dual-label) biotin-tagged α-FLAG monoclonal antibody (ThermoFisher Scientific #MA1-91878-BTIN ...
-
bioRxiv - Genetics 2023Quote: ... We used Gibson Assembly to add a 3x FLAG tag to the appropriate end and insert the tagged coding sequence into a pcDNA5/FRT vector (Thermo Fisher). We note that we overexpressed the propeptide of PSMB4 (removing amino acids 2-45).
-
bioRxiv - Molecular Biology 2023Quote: ... or 90 pmol of SBP-tagged eIF4A1 protein was incubated with 30 μl of Dynabeads M-270 Streptavidin (Thermo Fisher Scientific), which had been preequilibrated with equilibration buffer (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biophysics 2023Quote: Dextran-tagged Rhodamine-B (Rhod-Dex) with 10,000 Da and 70,000 Da molecular weights were from Invitrogen (Invitrogen 11466337 and 11590226). Mouse pre-osteoblast cells were cultured on 400 nm porous and non-porous surfaces in a 96-well plate 24 hours before the incubation with Rhod-Dex ...
-
bioRxiv - Developmental Biology 2023Quote: Fifty wildtype or myc-tagged Sox3 expressing animal pole cell explants were crosslinked for 15 minutes with 1% methanol-free formaldehyde (Life Technologies) and the reaction was quenched with 125 mM glycine in 0.5% PBST (Triton X-100) ...
-
bioRxiv - Biochemistry 2024Quote: FL α-syn was covalently tagged at its lysine residues using NHS-Rhodamine (ex. 552 nm, em. 575 nm, Thermo Scientific). The probe was added to 100 µM α-syn in ∼ 8-fold molar excess ...
-
bioRxiv - Microbiology 2024Quote: ... N-terminal FLAG-tagged CBP/p300 cDNA was amplified by PCR and was then inserted into pcDNA3.1 (Invitrogen Thermo, Carlsbad, CA).
-
bioRxiv - Biophysics 2020Quote: MDA-MB-231 breast cancer cells were cultured in modified Eagle’s medium (MEM-F10, c.c.pro) supplemented with 10% fetal calf serum (FCS, Invitrogen) and 2.5 mM L-glutamin (c.c.pro ...
-
bioRxiv - Biophysics 2020Quote: ... cell medium was replaced by 1 ml Leibovitz’s L-15 Medium (c.c.pro) containing 10% FCS and 25 nM Hoechst 33342 nucleic acid stain (Invitrogen) and incubated for 1 h at 37 °C before imaging.
-
bioRxiv - Cancer Biology 2021Quote: ... After washing three times with MACS buffer consisting of PBS supplemented with 5 % FCS (Thermo Fisher Scientic) and 2 mM EDTA to remove unbounded cells ...
-
bioRxiv - Immunology 2021Quote: ... and 10% fetal calf serum (FCS, Biowest) and minced into pieces of 1 mm3 in PBS (Gibco). The biopsies were enzymatically digested with 1 mg/mL collagenase IV (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: ... USA) supplemented with 10% fetal calf serum (FCS) and 1% penicillin-streptomycin solution (Invitrogen, Carlsbad, CA, USA) and were incubated at 37□ in a humidified atmosphere containing 5% CO2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were pre-incubated with anti-Fc receptor antibody (Thermal Fisher Scientific, Cat. No. 14-9161-73) at 1:20 ratio to block Fc receptor before staining ...
-
bioRxiv - Immunology 2021Quote: ... and the absorbance (OD492nm) was measured using a Multiskan-FC microplate reader (Thermo Fisher Scientific, Helsinki, Finland). Ligation of the recombinant proteins was compared to the SUMO control.
-
bioRxiv - Immunology 2019Quote: ... resuspended in William’s E medium containing 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific) and plated ...
-
bioRxiv - Microbiology 2019Quote: ... in Dulbecco’s Modified Eagle medium (DME) supplemented with 1% v/v fetal calf serum (FCS) (Invitrogen, Australia) and 1% v/v Glutamax (Invitrogen ...
-
bioRxiv - Developmental Biology 2020Quote: ... 1 % non-essential amino acids and sodium pyruvate and 30 % fetal calf serum (FCS) (Gibco, 10270-106). When cells reached confluency ...
-
bioRxiv - Neuroscience 2020Quote: ... The absorbance at 450 nm wavelength was read on a microplate reader (Multiskan FC, Thermo Fisher Scientific). Mean values were calculated for triplicate sets of standards and samples ...
-
bioRxiv - Biophysics 2020Quote: ... the substrate was washed with cell culture medium (DMEM w/glutamax, 10% FCS, 1% P/S; Gibco). 3T3 fibroblasts (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... Absorbance was then measured at 595 nm using a Multiskan™ FC microplate photometer (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2020Quote: ... supplemented with 10% FCS and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). Mammalian cells were incubated at 35°C in a humidified 5% CO2 incubator and insect cells at 28°C ...
-
bioRxiv - Microbiology 2020Quote: ... supplemented with 10% FCS and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). Mammalian cells were incubated at 35°C in a humidified 5% CO2 incubator and insect cells at 28°C ...
-
bioRxiv - Microbiology 2020Quote: ... the absorbance at 492 nm was measured with a microplate photometer (Multiskan FC, Thermo Fisher Scientific, USA).
-
bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS) (Cytiva) and 2mM L-glutamine (Thermo Fisher Scientific) at 37°C in humidified 5% CO2 ...
-
bioRxiv - Immunology 2022Quote: ... the enzymatic dissociation of the lamina propria was quenched by addition of 1ml of 100% FCS (ThermoFisher) and 80 μL of 0.5M EDTA and placed on ice for five minutes ...
-
bioRxiv - Immunology 2022Quote: ... After another round of Fc blocking on ice for 15 min with sera from mouse (Thermo Fisher) and rat (Thermo Fisher) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were recovered in Hanks’ balanced-salt solution (HBSS) supplemented with 1% fetal calf serum (FCS) (Gibco) or RPMI medium (Gibco ...
-
bioRxiv - Microbiology 2021Quote: Monocytic cell line U937 (ATCC® CRL1593.2™) cultured in RPMI (Thermoscientific) supplemented with 10% FCS (Invitrogen) was used in all experiments ...
-
bioRxiv - Cell Biology 2021Quote: ... Apical washes collected from ALI infections were thawed and serially diluted in DMEM with 1% FCS (Gibco), then added to cells and incubated for 2 hours before discarding and adding a 2.4% (w/v ...
-
bioRxiv - Molecular Biology 2022Quote: ... were purchased from DSMZ (www.cell-lines.de) and cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) with 10% fetal calf serum (FCS, Gibco) and Glutamax (ThermoFisher Scientific) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and immediately measured the mixture’s absorbance at 575nm using a microplate reader (Thermo Scientific Multiskan FC, USA).