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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: We subsequently rinsed with PBS-d (0.1M PBS [BR0014G, ThermoFisher Scientific, MA, USA] with 1% DMSO), performed a 30 minute permeabilization wash with permeabilization buffer (PBS-d and 2% Triton-X-100) ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed gradually into a gradient of (1/4) Neurobasal media (NB: based medium contained 1% Lglutamine, 1% N2 and 2% B27 (all from Invitrogen)) plus reduced gradient of (3/4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C (for P. miniata) or 20C (for P. regularis) in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Developmental Biology 2022Quote: ... for 5 minutes at RT and then split with 1:8 ratio into 6 well plates pre-coated with 1:200 diluted geltrex in DMEM/F12 medium (Thermo Scientific A1413201) for 30 minutes at 37C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: HEK293T cells were seeded at the density of 106 cells/well in a 6-well plate and were grown overnight in complete DMEM/F12 1:1 Glutamax ™ (Thermofisher, UK). On the next day ...
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bioRxiv - Developmental Biology 2024Quote: ... hESC were diluted at ratios between 1:6 and 1:50 using 0.5 mM EDTA-PBS (Thermo Fisher Scientific, catalog no. 15575020).
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bioRxiv - Cancer Biology 2019Quote: ... and 5-6 pieces per well were cultured in a 6-well culture plate (Fisher Scientific). For up to two weeks tissue pieces were cultured in Advanced Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2021Quote: ... using 6 mL of cell suspension and 6 mL of Prolong Gold Antifade mountant (ThermoFisher Scientific) that were mixed on an object glass and then covered with a special STED cover glass ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Plasma IL-6 levels were measured by mouse IL-6 ELISA kit (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2023Quote: ... Serum was run through an interleukin-6 (IL-6) enzyme-linked immunoabsorbent assay (ELISA) kit (Invitrogen). Successful maternal immune activation in offspring of Poly(I:C ...
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bioRxiv - Immunology 2022Quote: ... IL-6 measurement was performed using IL-6 Mouse Uncoated ELISA Kit (88-7064-22; Invitrogen).
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bioRxiv - Evolutionary Biology 2023Quote: ... into the packaging 293T cells (with molar ratio for Luc-T20: N: M/E: S as 3: 1: 1: 1) with LipofectamineTM 2000 (Thermo Fisher Scientific). At 24 and 48 hours post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... 4 µg of cDNA was transfected using a 1:1 ratio of cDNA: Lipofectamine 2000 (52887, Invitrogen) in OPTI-MEM transfection medium ...
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bioRxiv - Neuroscience 2020Quote: ... 4 μg of cDNA was transfected using a 1:1 ratio of cDNA: Lipofectamine 2000 (52887, Invitrogen) in OPTI-MEM transfection medium ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and washed once with 1 mL of EB and 4 times with 1 mL of PBS (Gibco). The sRNA were extracted from total/inputs and immunoprecipitated fractions using respectively Trizol and Trizol-LS ...
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bioRxiv - Microbiology 2021Quote: One-step RT-PCR was performed on 2ul total RNA using the SuperScript IV One-Step PCR kit (ThermoFisher, Catalog No. 12594025) either to amplify nucleotides 1232-1583 of the PPRV N gene using published primers NP3 and NP4 (Couacy-Hymann et al 2002 ...
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bioRxiv - Microbiology 2021Quote: ... Amplification of RNA samples was performed with one-step TaqMan RT-qPCR using the RNA UltraSense One-Step quantitative system (Thermo Fisher Scientific). The standard curve was generated by serial end-point dilution ...
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bioRxiv - Systems Biology 2020Quote: ... The samples corresponding to either one TMT11plex or one TMTPro set were then mixed in a single glass HPLC insert (ThermoFisher C4010-630) and dried down to dryness in a speed-vacuum (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR were performed with SuperScript III Platinum One-Step qRT-PCR kit (SuperScript™ III Platinum™ One-Step qRT-PCR kit, universal Invitrogen) using 5μL of nucleic acid extract and 20μL of RT-qPCR reagent mix using cycling parameters ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR were performed with SuperScript III Platinum One-Step qRT-PCR kit (SuperScript™ III Platinum™ One-Step qRT-PCR kit, universal Invitrogen) using 2.5μL of nucleic acid extract and 7.5μL of RT-qPCR reagent mix ...
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bioRxiv - Genomics 2022Quote: ... Plasma protein absorbance was measured at 280 nm using NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific, ND-ONE-W).
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bioRxiv - Developmental Biology 2024Quote: ... Triplicate reactions for each sample were prepared and the samples were run in a Step One Real-Time PCR Step One Real-Time PCR System Thermal Cycling Block (Applied Biosystems #4376357). The analysis of real-time data was performed using the included StepOne Software version 2.0 and graphs were generated using GraphPad Prism (GraphPad Software) ...
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bioRxiv - Microbiology 2023Quote: ... An 800 bp fragment covering the ORF10 region was amplified by one-step RT-PCR using the SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThermoFisher) and the following primers (OL11F ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μl of extracted RNA samples were determined in one-step RT-qPCR using AgPath-ID one-step RT-PCR (Applied Biosystems, UK) with the following cycle conditions ...
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bioRxiv - Genomics 2024Quote: ... Emulsion PCR for sequence template synthesis was performed using the Ion PITM Hi-QTM OT2 200 Kit v2 with the Ion One TouchTM II System and the Ion One TouchTM II ES (Thermo Fisher Scientific). Sequencing was performed using the Ion ProtonTM System with Ion PITM Chip kit v3 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... the extracted vRNA was converted to double-stranded cDNA and amplified using a one-step RT-PCR using SuperScript III One-Step RT-PCR kit (Thermo Fisher Scientific). The primers used were as follows ...
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bioRxiv - Genomics 2024Quote: ... Emulsion PCR for sequence template synthesis was performed with the Ion PITM Hi-QTM OT2 200 Kit v2 using the Ion One TouchTM II System and the Ion One TouchTM II ES (Thermo Fisher Scientific). Sequencing was performed using the Ion PITM Chip kit v3 and the Ion ProtonTM System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... D-glucose free medium was prepared using D-glucose free DMEM (Thermo-Fisher Scientific, USA) supplemented with 10 % dialyzed FBS (Thermo-Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5-1×104 cells per well were seeded in 6-well Falcon plates (Fisher Scientific, Cat #087721B) in triplicate ...
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bioRxiv - Genomics 2022Quote: ... Cells were routinely passaged 1:6 once they reached 75% confluency using 0.25% trypsin-EDTA (Gibco, 25200056) for HEK293T cells ...
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bioRxiv - Neuroscience 2022Quote: ... Huh7 or SH-SY5Y cells in 6-well plates were incubated with 1 μM puromycin (Thermo Fisher) for 10 min at 32°C (STHdh ...
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bioRxiv - Genetics 2022Quote: ... A mix of 6 ul of nick translated RNA probe with 12ul of Human Cot-1 (ThermoFisher) and 2ul of salmon testes (ThermoFisher ...
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bioRxiv - Biophysics 2024Quote: ... 1 filter paper for ~6 seconds with blot force 0 on a Vitrobot Mark IV (ThermoFisher Scientific) at 100% humidity and 22°C.
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bioRxiv - Cell Biology 2024Quote: ... Overexpressed SAS-6 was detected with mouse anti-c-Myc (1:250; 9E10; Invitrogen, UK; 13-2500) antibody ...
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bioRxiv - Cell Biology 2023Quote: ... Hepa 1-6 and HuH-7 were grown in Dulbecco’s modified Eagle’s medium–high glucose (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... with 4% sucrose/4% formaldehyde (ThermoFisher Scientific) in 1X PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Detection of SARS-CoV-2 N1 gene sequences and host RNase P mRNA from de-identified SARS-CoV-2 positive samples was carried out with the one-step TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher), using 2 μL of undiluted PEARL extract ...
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bioRxiv - Biochemistry 2020Quote: ... one µg of small RNAs was mixed with 2 µg of HPDP-Biotin (ThermoFisher Scientific, 1 mg/ml in DMSO). The reaction was incubated for three hours at room temperature protected from light and mixed every 15 minutes ...
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bioRxiv - Genetics 2019Quote: ... BbsI-linearized piggyBac-U6 backbone was mixed with purified PCE product in a vector:insert 2:1 ratio and was directly used for transformation of One Shot MAX Efficiency DH5α-T1 Competent Cells (Invitrogen).
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bioRxiv - Microbiology 2019Quote: ... The concentration of ribosomes was inferred by measuring the absorbance of the sample at 260 nm (1 A260 = 60 μg/ml or 24 nM) with a NanoDrop One (ThermoFisher). For analytical purposes ...
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bioRxiv - Developmental Biology 2020Quote: ... The following secondary antibodies from Thermo Fisher Scientific were incubated at room temperature for one hour: Donkey anti-rat Alexa488 (1:100, Thermo Fisher, Cat # A-21208), Donkey anti-rat Alexa594 (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... dried peptide sample was resuspended in 200 mM HEPES (pH 8) and incubated for 1 h at room temperature with one of the TMT10-plex reagents (ThermoFisher) solubilized in 100% anhydrous ACN ...
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bioRxiv - Systems Biology 2020Quote: ... One bottle of the Express Five medium (1 liter) was supplemented with 90 ml of L-Glutamine (200 mM, Invitrogen). During cultivation ...
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bioRxiv - Microbiology 2021Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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Genomic and phylogenetic analyses of SARS-CoV-2 strains isolated in the city of Gwangju, South KoreabioRxiv - Microbiology 2020Quote: ... 1 μL of 25X RT-PCR enzyme mixture included in AgPath-ID One-step RT-PCR Reagents (Thermo Fisher Scientific), 5 μL of RNA ...
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bioRxiv - Microbiology 2020Quote: ... consisting of lysis enhancer and resuspension buffer at a 1:10 ratio (both from the CellsDirect One-Step RT-qPCR kit; Invitrogen). Next ...
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bioRxiv - Plant Biology 2021Quote: ... The integrity of the RNA was verified on 1% agarose gel and quantified on the NanoDrop One spectrophotometer (Thermo Fisher). All samples used showed a ratio reading 1.8-2.0 of absorbance at 260/280 nm and 260/230 nm for high-quality RNA.
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bioRxiv - Microbiology 2022Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...