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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and then normalized using the high-throughput Invitrogen SequalPrep 96-well Plate Kit (Thermo Fisher Scientific Corp.). Samples were then pooled to make one library ...
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bioRxiv - Systems Biology 2024Quote: ... Amplicons from each 96-well plate were pooled and purified using the PCR Purification Kit (ThermoFisher K310002). Each pooled sample was prepared as a single sequencing library by the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Indexed PCR products were purified and normalized using the SequalPrep Normalization Plate Kit (Invitrogen, Carlsbad CA, USA), subsequently quantified using the Qubit dsDNA HS Kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 4 wells of each plate were transduced with the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher) using the recommended MOIs for each factor (KOS=5 ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: RNA was extracted from cells cultured in 12-well plates using the Purelink RNA Mini Kit (Invitrogen). DNA was removed by incubating the preparation on the column with Purelink DNase (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... and GST-fusions were purified on glutathione-agarose and conjugated to Alexa Fluor 594 succinimidyl esters (red) (Molecular Probes, Thermo Fisher Scientific). Approximately 106 mature cysts or cyst walls were washed in PBS and fixed in 1% paraformaldehyde buffered with phosphate for 15 min at RT ...
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bioRxiv - Microbiology 2019Quote: ... and GST-fusions were purified on glutathione-agarose and conjugated to Alexa Fluor 594 succinimidyl esters (red) (Molecular Probes, Thermo Fisher Scientific). Approximately 106 mature cysts or cyst walls were washed in PBS and fixed in 1% paraformaldehyde buffered with phosphate for 15 min at RT ...
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bioRxiv - Immunology 2023Quote: Removal of the hexa-glutamate tag was carried out via 3C protease-based cleavage (ACRO Biosystems) for 24 hours at 4 °C followed by subtraction of the GST-tagged 3C protease via glutathione agarose (Thermo Fisher Scientific) and gel filtration (Superdex 200 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysates were obtained via centrifugation at 20,000 ×g for 10 min at 4°C and were incubated with Purified GST-RhoA (G17A)-glutathione magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides were incubated with the indicated primary antibodies as described below and developed with Ultravision ONE Detection System kit (Thermo Scientific. TL-015-HAJ) using Permanent Mounting Medium (Prolong ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were azide-labeled using Click-iT O-GlcNAc Enzymatic Labeling System and reacted with biotin alkyne using Click-IT Biotin Protein Analysis Detection Kit (Thermo Fisher Scientific, Waltham, MA) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Labeling and detection of apoptotic cells was completed using the Invitrogen Click-iT Plus TUNEL Assay for In Situ Apoptosis Detection 488 kit (Invitrogen, Waltham, MA, catalog #: C10617) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The Click-iT™ Plus TUNEL Assay kit for in situ apoptosis detection with Alexa Fluor488 dyes was purchased from Invitrogen (Catalog Nos. C10617). The labeling protocol was as instructed by the manufacture’s manual.
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bioRxiv - Immunology 2022Quote: We estimated hemocyte differentiation by visualizing and quantifying the synthesis of new DNA in vivo based on 5-ethynyl-2′-deoxyuridine (EdU) incorporation and subsequent detection using the Click-iT EdU Alexa Fluor 647 kit (Invitrogen, Grand Island, NY, USA) as previously described (36,37) ...
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bioRxiv - Molecular Biology 2022Quote: ... of EdU-labeled adult worms and schistosomula were subjected to EdU detection using a Click-iT™ EdU Cell Proliferation Kit (Alexa Fluor™ 488 dye) (Thermo Fisher Scientific) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2024Quote: Flow cytometry-based detection of early and late apoptosis of cancer cells was conducted using the Annexin-V apoptosis assay kit (Thermofisher Scientific Cat. No. V13241). Staining of cells with Annexin-V and propidium iodide was conducted according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Cell death was assessed using the Click-iT™ Plus TUNEL Assay for In Situ Apoptosis Detection Alexa Fluor™ 647 Imaging Kit (Life Technologies, C10617). Unless otherwise stated ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RAS activity was determined by the Active Ras Pull-Down and Detection Kit according to the manufacturer’s instructions (16117, Thermo Fisher Scientific, Lafayette, CO, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Labeling and detection of apoptotic cells were completed using the Invitrogen Click-iT Plus TUNEL Assay for In Situ Apoptosis Detection 488 kit (Invitrogen, Waltham, MA, catalog #: C10617) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was performed to assess cell apoptosis using commercial apoptosis detection kit (Thermo Fisher Scientific, United States). All procedures were done according to the directions of the manufacturers ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were then rinsed in PBSTx then incubated at 37°C in TdT reaction buffer from Click-iT™ Plus TUNEL Assay for In Situ Apoptosis Detection kit (Thermo Fisher Scientific C10617) for one hour ...
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bioRxiv - Cell Biology 2020Quote: The calibration was verified using a phase retrieval algorithm [20] in combination with through-focus scans of 175 nm green fluorescent beads (PS-speck, ThermoFisher, P7220, dilution 1:500) mounted in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... we added 1:20-1:100 (volume) of 1 mg/ml fluorescent tag (Alexa Fluor® succinimidyl esters of the desired excitation/emission spectrum, Invitrogen™, Molecular Probes®) to the gel premix immediately before polymerization.
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... The fluorescent secondary antibodies used in this study were Alexa Fluor 568 goat anti-human IgG(H+L) (Molecular Probes, A-21090, 1:1000), Alexa Fluor 488 goat anti-mouse IgG(H+L ...
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bioRxiv - Neuroscience 2020Quote: Each organotypic slice was incubated for 1 h prior to imaging with the fluorescent Ca2+-indicator dye Fluo-4-AM (Molecular Probes, Invitrogen, Carlsbad, CA, USA). The conditions for incubating the dye were standardized across different slices to avoid uneven dye loading ...
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bioRxiv - Bioengineering 2021Quote: ... three different volumes (20 µL, 40 µL, and 60 µL) of a suspension of 1-µm diameter fluorescent particles (FluoroMax, Thermo Fisher Scientific Inc., USA) at a concentration of 2×107 particles/mL was added in the inlet reservoirs ...
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bioRxiv - Neuroscience 2019Quote: ... immunofluorescence analysis of RYR1 and AR were performed in 10 μm quadriceps cryosections using the same antibodies listed above supplemented with fluorescent WGA (Thermo Scientific W11261, 1:500). Images were collected with an epifluorescence microscope (Leica CTR6000 ...
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bioRxiv - Neuroscience 2020Quote: ... incubated with fluorescent-conjugated secondary antibodies [Alexa Fluor 488 Goat anti-Rabbit IgG (1:500 dilution; catalog # A-11034; Thermo Fisher Scientific, Tokyo, Japan)] diluted with the blocking solution for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The cultures were then washed three times with PBS and then incubated for two hours with the following fluorescent secondary antibodies: Alexa Fluor 568-conjugated donkey anti mouse (1:2000, Life Technologies-Invitrogen, Carlsbad, CA, USA), Alexa Fluor 488-conjugated donkey anti rabbit (1:2000 ...
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CREB5 reprograms nuclear interactions to promote resistance to androgen receptor targeting therapiesbioRxiv - Cancer Biology 2021Quote: ... 0.1% Tween (PBST) and incubated with fluorescent anti-rabbit or anti-mouse secondary antibodies at a dilution of 1:5000 (Thermo Fisher Scientific, NC9401842 (Rabbit) and NC0046410 (mouse) ...
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bioRxiv - Microbiology 2020Quote: ... 500 nm excitation/550 nm emission) and MV RNA was determined by using the membrane permeable SYTO RNASelect green fluorescent cell stain (Invitrogen, 1 μM final concentration) to stain 10 μL of each fraction ...
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bioRxiv - Neuroscience 2021Quote: ... for at least 20 min at room temperature before staining with a fluorescent myelin stain (FluoroMyelinTM Green; 1:300 in PBS; Molecular probes, Eugene, OR, USA) for 20 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... the planktonic cells were aspirated and biofilms were gently washed twice with PBS and stained with fluorescent dye FUN-1 (Invitrogen, Thermo Fisher Scientific, ZA) and concanavalin A (ConA)-Alexa Fluor 488 conjugate (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Some of the sections were counterstained with NeuroTrace™ 435/455 Blue or NeuroTrace™ 530/615 Red Fluorescent Nissl Stain (1:100, N21479 or N21482, Thermo Fisher Scientific) in PBS-X ...
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bioRxiv - Neuroscience 2023Quote: ... specific fluorescent immunostainings were visualized using Alexa488-conjugated anti-sheep IgG and Alexa546-conjugated anti-rabbit IgG (1:300; Molecular Probes, Eugene, OR, USA).
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bioRxiv - Neuroscience 2023Quote: Sections were washed in PBS three times for 5 min each and incubated in secondary antibodies in PBS (goat or donkey anti-rabbit, anti-mouse, or anti-rat fluorescent antibodies; Invitrogen Alexa Fluor; 1:500) for 1 h at room temperature in the dark ...
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bioRxiv - Neuroscience 2023Quote: Slices were stained for green fluorescent protein (GFP) with a rabbit monoclonal anti-GFP antibody (G10362, Molecular Probes, 1:1000 dilution in blocking buffer) and for tyrosine hydroxylase (TH ...
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bioRxiv - Immunology 2020Quote: ... – 80 wells per plate and 2 plates (AK1,2-3,4)– 96 wells per plate) 21 ml of Opti-Mem (Gibco) was mixed with DNA ...
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bioRxiv - Microbiology 2022Quote: ... RNase-free (1 U/µl) kit (ThermoFisher) prior to reverse transcription reaction ...
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bioRxiv - Bioengineering 2023Quote: ... Commercial calcium Calibration Buffer Kit #1 (Invitrogen Life Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in precoated collagen plates using DMEM / Ham’s F12 (1:1) medium supplemented with 2mM Glutamine (Life Technologies), 250ng/ml insulin solution from bovine pancreas (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... and cultured in a 24-well plate overlaid with basal media (Advanced DMEM with 1% glutamax and 1% pen/strep) containing B-27 (50X, ThermoFisher), N-2 ...
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bioRxiv - Biochemistry 2020Quote: HEK293F cells were seeded in 24 well plates (1 million cells in each well) in a final volume of 1 mL of FreeStyleTM 293 medium (GIBCO). The cells were transfected with 1 ug DNA as described before and incubated at 37°C and in 8% CO2 ...
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bioRxiv - Cell Biology 2021Quote: E14 mES cells were cultured on 0.1% gelatin-coated plates with MEF feeder cells in N2/B27/LIF/2i medium (1:1 mix of DMEM/F12 (11320-033, Gibco) and Neurobasal medium (21103-049 ...
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bioRxiv - Plant Biology 2020Quote: ... Seven day wild type and nip2;1 seedlings were transferred from MS media to 1.5% agarose plates containing 60 mg L-1 Bromocresol Purple (Acros Organics) and 1mM CaSO4 in sterile distilled water with the pH adjusted to 5.7 using KOH ...
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bioRxiv - Biochemistry 2022Quote: ... expression clone cDNA or control cDNA (1 µg) was mixed with packaging plasmids MD2G (1 µg) and PSPAX2 (1 ug) in 600 µL per plate OPTIMEM (Gibco) and Lipofectamine™ 2000 transfection reagent (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: HEK293F cells were seeded in 24-well plates (1 million cells in each well) in a final volume of 1 ml of FreeStyleTM 293 medium (GIBCO). The cells were transfected with 1 µg DNA of different hSARM1 constructs and incubated at 37°C and in 8% CO2 ...
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bioRxiv - Immunology 2022Quote: ... and HFs were placed individually into a 96-well plate slot with 100 µl supplemented medium (William’s E plus 1% penicillin-streptomycin antibiotics, 1% L-glutamine (Invitrogen-Gibco), 0.01% hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... mESCs were maintained on gelatin-coated plates in 2i/LIF medium containing a 1:1 mix of DMEM/F-12 (Gibco) and Neurobasal medium (Gibco ...
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bioRxiv - Immunology 2021Quote: ... 1.75x103 cells were loaded into individual well of a 96 well v-bottom plate in RPMI/5% (v/v) FBS/2mM EDTA/1% (v/v) Penicillin/Streptomycin/1% (v/v) L-Glutamine (ThermoFisher)/0.1% (v/v ...